Select and Antiselect

The exon/intron structure of the gene in the EM:D55676 EMBL record has been experimentally determined. BlastX homology to another gamma-glutamylcysteine synthetase from Rattus norvegicus confirms this structure (click on the BlastX boxes to see this).

To predict this gene with GeneFinder it would be possible to modify AutoFind Parameters until it was found. However, GeneFinder can be forced to use or disregard selected features. To do this, press the Clear button to remove all highlighting of features. Select the ATG (base 684-686)(see image below) with the right mouse button and a menu appears. Choose [Select] from the menu and the ATG is highlighted in green. Now select the splice donor (base 1034-1035) with the right mouse button and choose [Antiselect]. The donor is hightlighted in pale green. From the GeneFind.. menu choose [AutoFind gene]. The gene prediction is the same as that in the EMBL record.

Note that Unselect is also available from the feature selection menu.

Go to Step 6


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