The exon/intron structure of the gene in the EM:D55676 EMBL record has
been experimentally determined. BlastX homology to another
gamma-glutamylcysteine synthetase from Rattus norvegicus
confirms this structure (click on the BlastX boxes to see this).
To predict this gene with GeneFinder it would be possible to modify
AutoFind Parameters until it was found. However, GeneFinder can be
forced to use or disregard selected features. To do this, press the
Clear button to remove all highlighting of features. Select the ATG
(base 684-686)(see image below) with the right mouse button and a menu
appears. Choose [Select] from the menu and the ATG is highlighted in
green. Now select the splice donor (base 1034-1035) with the right
mouse button and choose [Antiselect]. The donor is hightlighted in
pale green. From the GeneFind.. menu choose [AutoFind gene]. The gene
prediction is the same as that in the EMBL record.
Note that Unselect is also available from the feature selection menu.