/*************************************************************************** ** ** 454 Life Sciences Corporation ** Newbler Metrics Results ** ** Date of Assembly: 2009/04/01 14:11:14 ** Project Directory: /lustre/pathogen/work/team81/jit/LEISH/Leishmania.010409 ** Software Release: 2.0.00.22 ** ***************************************************************************/ /* ** Input information. */ runData { file { path = "/lustre/pathogen/work/team81/jit/LEISH/454_READS_SFF/FSGO63H01.sff"; numberOfReads = 33365, 33364; numberOfBases = 5654617, 5618382; } file { path = "/lustre/pathogen/work/team81/jit/LEISH/454_READS_SFF/FSGO63H02.sff"; numberOfReads = 33598, 33594; numberOfBases = 5719527, 5678460; } file { path = "/lustre/pathogen/work/team81/jit/LEISH/454_READS_SFF/FSGO63H03.sff"; numberOfReads = 35399, 35394; numberOfBases = 5877014, 5832651; } file { path = "/lustre/pathogen/work/team81/jit/LEISH/454_READS_SFF/FSGO63H04.sff"; numberOfReads = 32537, 32532; numberOfBases = 5470105, 5430327; } } pairedReadData { file { path = "/lustre/pathogen/work/team81/jit/LEISH/454_READS_SFF/FLVWW5T02.sff"; numberOfReads = 264545, 376384; numberOfBases = 47295221, 37620112; numWithPairedRead = 120206; } file { path = "/lustre/pathogen/work/team81/jit/LEISH/454_READS_SFF/FP8LRLE01.sff"; numberOfReads = 87450, 134075; numberOfBases = 28604420, 23989998; numWithPairedRead = 51145; } file { path = "/lustre/pathogen/work/team81/jit/LEISH/454_READS_SFF/FPJBGA001.sff"; numberOfReads = 232477, 275412; numberOfBases = 58977049, 36185772; numWithPairedRead = 104252; } file { path = "/lustre/pathogen/work/team81/jit/LEISH/454_READS_SFF/FQRGO9E01.sff"; numberOfReads = 682686, 1059770; numberOfBases = 239566367, 202400321; numWithPairedRead = 412339; } file { path = "/lustre/pathogen/work/team81/jit/LEISH/454_READS_SFF/FQRGO9E02.sff"; numberOfReads = 497205, 768448; numberOfBases = 179962941, 151880807; numWithPairedRead = 298861; } file { path = "/lustre/pathogen/work/team81/jit/LEISH/454_READS_SFF/FLVWW5T01.sff"; numberOfReads = 430207, 658207; numberOfBases = 89519334, 71822221; numWithPairedRead = 239358; } } /* ** Operation metrics. */ runMetrics { totalNumberOfReads = 3407180; totalNumberOfBases = 546459051; numberSearches = 227503; seedHitsFound = 95314903, 418.96; overlapsFound = 9317866, 40.96, 9.78%; overlapsReported = 8153860, 35.84, 8.55%; overlapsUsed = 4150408, 18.24, 50.90%; } readAlignmentResults { file { path = "/lustre/pathogen/work/team81/jit/LEISH/454_READS_SFF/FSGO63H01.sff"; numAlignedReads = 33016, 98.96%; numAlignedBases = 5112247, 90.99%; inferredReadError = 1.51%, 77450; } file { path = "/lustre/pathogen/work/team81/jit/LEISH/454_READS_SFF/FSGO63H02.sff"; numAlignedReads = 33228, 98.91%; numAlignedBases = 5163779, 90.94%; inferredReadError = 1.51%, 78135; } file { path = "/lustre/pathogen/work/team81/jit/LEISH/454_READS_SFF/FSGO63H03.sff"; numAlignedReads = 35012, 98.92%; numAlignedBases = 5292657, 90.74%; inferredReadError = 1.54%, 81771; } file { path = "/lustre/pathogen/work/team81/jit/LEISH/454_READS_SFF/FSGO63H04.sff"; numAlignedReads = 32161, 98.86%; numAlignedBases = 4925853, 90.71%; inferredReadError = 1.55%, 76135; } } pairedReadResults { file { path = "/lustre/pathogen/work/team81/jit/LEISH/454_READS_SFF/FLVWW5T02.sff"; numAlignedReads = 366905, 97.48%; numAlignedBases = 34996272, 93.03%; inferredReadError = 1.52%, 532262; numberWithBothMapped = 99533; numWithOneUnmapped = 4498; numWithMultiplyMapped = 16054; numWithBothUnmapped = 121; } file { path = "/lustre/pathogen/work/team81/jit/LEISH/454_READS_SFF/FP8LRLE01.sff"; numAlignedReads = 132854, 99.09%; numAlignedBases = 22804630, 95.06%; inferredReadError = 0.85%, 193733; numberWithBothMapped = 45795; numWithOneUnmapped = 884; numWithMultiplyMapped = 4450; numWithBothUnmapped = 16; } file { path = "/lustre/pathogen/work/team81/jit/LEISH/454_READS_SFF/FPJBGA001.sff"; numAlignedReads = 270553, 98.24%; numAlignedBases = 34084603, 94.19%; inferredReadError = 1.11%, 379699; numberWithBothMapped = 90031; numWithOneUnmapped = 2550; numWithMultiplyMapped = 11423; numWithBothUnmapped = 248; } file { path = "/lustre/pathogen/work/team81/jit/LEISH/454_READS_SFF/FQRGO9E01.sff"; numAlignedReads = 1050035, 99.08%; numAlignedBases = 192760591, 95.24%; inferredReadError = 0.77%, 1482136; numberWithBothMapped = 371254; numWithOneUnmapped = 6756; numWithMultiplyMapped = 34181; numWithBothUnmapped = 148; } file { path = "/lustre/pathogen/work/team81/jit/LEISH/454_READS_SFF/FQRGO9E02.sff"; numAlignedReads = 759857, 98.88%; numAlignedBases = 144632907, 95.23%; inferredReadError = 0.72%, 1046480; numberWithBothMapped = 269165; numWithOneUnmapped = 5016; numWithMultiplyMapped = 24069; numWithBothUnmapped = 611; } file { path = "/lustre/pathogen/work/team81/jit/LEISH/454_READS_SFF/FLVWW5T01.sff"; numAlignedReads = 643520, 97.77%; numAlignedBases = 67118519, 93.45%; inferredReadError = 1.24%, 830577; numberWithBothMapped = 201736; numWithOneUnmapped = 7791; numWithMultiplyMapped = 29611; numWithBothUnmapped = 220; } } /* ** Consensus distribution information. */ consensusDistribution { fullDistribution { signalBin = 0.0, 247134; signalBin = 0.1, 11; signalBin = 0.2, 7; signalBin = 0.3, 6; signalBin = 0.4, 8; signalBin = 0.5, 161131; signalBin = 0.6, 110669; signalBin = 0.7, 80650; signalBin = 0.8, 888853; signalBin = 0.9, 14697460; signalBin = 1.0, 7267417; signalBin = 1.1, 140475; signalBin = 1.2, 8124; signalBin = 1.3, 1872; signalBin = 1.4, 765; signalBin = 1.5, 955; signalBin = 1.6, 2692; signalBin = 1.7, 35166; signalBin = 1.8, 656673; signalBin = 1.9, 3006912; signalBin = 2.0, 1180682; signalBin = 2.1, 61514; signalBin = 2.2, 3541; signalBin = 2.3, 742; signalBin = 2.4, 494; signalBin = 2.5, 1291; signalBin = 2.6, 5941; signalBin = 2.7, 39327; signalBin = 2.8, 222582; signalBin = 2.9, 502371; signalBin = 3.0, 244633; signalBin = 3.1, 32253; signalBin = 3.2, 3496; signalBin = 3.3, 934; signalBin = 3.4, 1066; signalBin = 3.5, 3197; signalBin = 3.6, 9847; signalBin = 3.7, 30484; signalBin = 3.8, 72673; signalBin = 3.9, 98048; signalBin = 4.0, 67475; signalBin = 4.1, 20680; signalBin = 4.2, 4419; signalBin = 4.3, 1418; signalBin = 4.4, 1348; signalBin = 4.5, 2476; signalBin = 4.6, 4688; signalBin = 4.7, 9708; signalBin = 4.8, 19619; signalBin = 4.9, 29730; signalBin = 5.0, 21545; signalBin = 5.1, 6781; signalBin = 5.2, 1815; signalBin = 5.3, 1010; signalBin = 5.4, 1108; signalBin = 5.5, 1606; signalBin = 5.6, 2204; signalBin = 5.7, 3294; signalBin = 5.8, 4421; signalBin = 5.9, 5102; signalBin = 6.0, 4353; signalBin = 6.1, 2236; signalBin = 6.2, 997; signalBin = 6.3, 641; signalBin = 6.4, 701; signalBin = 6.5, 793; signalBin = 6.6, 1031; signalBin = 6.7, 1156; signalBin = 6.8, 1293; signalBin = 6.9, 1125; signalBin = 7.0, 843; signalBin = 7.1, 652; signalBin = 7.2, 520; signalBin = 7.3, 554; signalBin = 7.4, 586; signalBin = 7.5, 676; signalBin = 7.6, 744; signalBin = 7.7, 796; signalBin = 7.8, 767; signalBin = 7.9, 746; signalBin = 8.0, 753; signalBin = 8.1, 743; signalBin = 8.2, 730; signalBin = 8.3, 696; signalBin = 8.4, 724; signalBin = 8.5, 762; signalBin = 8.6, 722; signalBin = 8.7, 728; signalBin = 8.8, 674; signalBin = 8.9, 633; signalBin = 9.0, 679; signalBin = 9.1, 589; signalBin = 9.2, 588; signalBin = 9.3, 525; signalBin = 9.4, 476; signalBin = 9.5, 468; signalBin = 9.6, 438; signalBin = 9.7, 426; signalBin = 9.8, 431; signalBin = 9.9, 374; signalBin = 10.0, 295; signalBin = 10.1, 275; signalBin = 10.2, 292; signalBin = 10.3, 253; signalBin = 10.4, 238; signalBin = 10.5, 219; signalBin = 10.6, 191; signalBin = 10.7, 178; signalBin = 10.8, 165; signalBin = 10.9, 150; signalBin = 11.0, 122; signalBin = 11.1, 127; signalBin = 11.2, 109; signalBin = 11.3, 104; signalBin = 11.4, 84; signalBin = 11.5, 92; signalBin = 11.6, 63; signalBin = 11.7, 58; signalBin = 11.8, 54; signalBin = 11.9, 60; signalBin = 12.0, 43; signalBin = 12.1, 39; signalBin = 12.2, 43; signalBin = 12.3, 30; signalBin = 12.4, 47; signalBin = 12.5, 28; signalBin = 12.6, 28; signalBin = 12.7, 18; signalBin = 12.8, 23; signalBin = 12.9, 16; signalBin = 13.0, 19; signalBin = 13.1, 14; signalBin = 13.2, 11; signalBin = 13.3, 8; signalBin = 13.4, 15; signalBin = 13.5, 19; signalBin = 13.6, 7; signalBin = 13.7, 6; signalBin = 13.8, 3; signalBin = 13.9, 5; signalBin = 14.0, 6; signalBin = 14.1, 10; signalBin = 14.2, 6; signalBin = 14.3, 3; signalBin = 14.4, 6; signalBin = 14.5, 7; signalBin = 14.6, 4; signalBin = 14.7, 1; signalBin = 14.8, 3; signalBin = 15.0, 3; signalBin = 15.1, 2; signalBin = 15.2, 5; signalBin = 15.3, 1; signalBin = 15.4, 1; signalBin = 15.5, 2; signalBin = 15.6, 1; signalBin = 15.7, 1; signalBin = 16.0, 5; signalBin = 16.3, 1; signalBin = 16.4, 1; signalBin = 16.5, 1; signalBin = 16.6, 1; signalBin = 16.7, 1; signalBin = 16.9, 1; signalBin = 17.4, 3; signalBin = 18.4, 1; signalBin = 19.4, 1; } distributionPeaks { signalPeak = 1, 0.98; signalPeak = 2, 1.96; signalPeak = 3, 2.94; signalPeak = 4, 3.92; signalPeak = 5, 4.94; signalPeak = 6, 5.90; signalPeak = 7, 6.84; signalPeak = 8, 7.84; signalPeak = 9, 8.78; signalPeak = 10, 9.68; signalPeak = 11, 10.54; signalPeak = 12, 11.42; } thresholdsUsed { threshold = 0, 1, 0.28; threshold = 1, 2, 1.48; threshold = 2, 3, 2.40; threshold = 3, 4, 3.38; threshold = 4, 5, 4.38; threshold = 5, 6, 5.34; threshold = 6, 7, 6.32; threshold = 7, 8, 7.08; threshold = 8, 9, 8.30; threshold = 9, 10, 9.66; threshold = 10, 11, 10.20; threshold = 11, 12, 11.06; interpolationAmount = 0.98; } } /* ** Consensus results. */ consensusResults { readStatus { numAlignedReads = 3357141, 98.53%; numAlignedBases = 516892058, 94.59%; inferredReadError = 0.92%, 4778378; numberAssembled = 3049214; numberPartial = 65827; numberSingleton = 50039; numberRepeat = 237959; numberOutlier = 4141; numberTooShort = 0; } pairedReadStatus { numberWithBothMapped = 1077514; numberWithOneUnmapped = 27495; numberMultiplyMapped = 119788; numberWithBothUnmapped = 1364; library { libraryName = "FLVWW5T02.sff"; pairDistanceAvg = 2915.3; pairDistanceDev = 728.8; } library { libraryName = "FP8LRLE01.sff"; pairDistanceAvg = 3151.3; pairDistanceDev = 787.8; } library { libraryName = "FPJBGA001.sff"; pairDistanceAvg = 3074.9; pairDistanceDev = 768.7; } library { libraryName = "FQRGO9E01.sff"; pairDistanceAvg = 3130.2; pairDistanceDev = 782.5; } library { libraryName = "FQRGO9E02.sff"; pairDistanceAvg = 3119.6; pairDistanceDev = 779.9; } library { libraryName = "FLVWW5T01.sff"; pairDistanceAvg = 2912.4; pairDistanceDev = 728.1; } } scaffoldMetrics { numberOfScaffolds = 119; numberOfBases = 30970487; avgScaffoldSize = 260256; N50ScaffoldSize = 561746; largestScaffoldSize = 2393801; } largeContigMetrics { numberOfContigs = 5138; numberOfBases = 29934682; avgContigSize = 5826; N50ContigSize = 10298; largestContigSize = 73993; Q40PlusBases = 29630522, 98.98%; Q39MinusBases = 304160, 1.02%; } allContigMetrics { numberOfContigs = 6765; numberOfBases = 30371395; } }