Predicted Targets of mmu-miR-25 Based on Experimental Evidence
Illumina Probe IDs Gene Symbols Gene Name Entrez ID Log Fold Change upon Dgcr8 Depletion P-value of change upon Dgcr8 Depletion Log Fold Change upon miR-25 Reintroduction P-value of change upon miR-25 Reintroduction GO Terms KEGG Pathways Transcript used for 3'UTR seed counts 6mer(1A)-GCAATA 6mer(3)-GTGCAA 6mer(2)-TGCAAT 7mer(2)(m8)-GTGCAAT 7mer(1A)-TGCAATA 8mer(1A)-GTGCAATA Target Scan Conserved Target Context Score Target Scan Non-conserved Target Context Score miRBase Targets P-value
iXuHlOb1FICWdz.rd4Tmem184btransmembrane protein 184b2236931.231.0e-180.436.4e-05 ENSMUST00000074991 1 1 1 1 1 1-0.1188Not FoundNot Found
Bp5e4GiPe018Rk3.L4Adam23a disintegrin and metallopeptidase domain 23237921.264.2e-160.711.2e-05cell adhesion(GO:0007155) | proteolysis(GO:0006508) | integrin-mediated signaling pathway(GO:0007229) OTTMUST00000004901 2 2 5 2 2 1-0.3949Not FoundNot Found
lkuSKIB557us.i4.5IPlekhm1pleckstrin homology domain containing, family M (with RUN domain) member 13530470.945.5e-160.983.2e-05intracellular signaling cascade(GO:0007242) OTTMUST00000005762 2 2 2 1 2 1-0.1376-0.3862Not Found
Ej5ewT_J98eO7nrqEoWhsc1l1Wolf-Hirschhorn syndrome candidate 1-like 1 (human)2341350.663.9e-150.562.0e-05transcription(GO:0006350) | regulation of transcription, DNA-dependent(GO:0006355) | chromatin modification(GO:0016568) ENSMUST00000084026 1 3 3 1 1 0Not FoundNot FoundNot Found
0Xul8aOr_34e.kHjnk uLp8e6gnXujLOheU7oRab8bRAB8B, member RAS oncogene family2354420.614.7e-150.897.3e-08transport(GO:0006810) | small GTPase mediated signal transduction(GO:0007264) | protein transport(GO:0015031) ENSMUST00000041139 1 2 1 1 1 1Not Found-0.342Not Found
oKJsk.kySo_5ezce7UGcnt2glucosaminyl (N-acetyl) transferase 2, I-branching enzyme145380.761.1e-130.451.2e-04 Glycosphingolipid biosynthesis - neo-lactoseries(00602) | Glycan structures - biosynthesis 2(01031)ENSMUST00000110191 1 0 2 0 1 0Not Found-0.046Not Found
3uKIKTKCuIcniH3tOkBicd2bicaudal D homolog 2 (Drosophila)768950.639.8e-120.391.6e-04microtubule-based movement(GO:0007018) ENSMUST00000048544 0 1 2 0 0 0Not FoundNot FoundNot Found
EeMSH_Qure_2ep49104931407G18RikRIKEN cDNA 4931407G18 gene709770.759.2e-110.573.3e-05 OTTMUST00000052375 0 0 0 0 0 0Not FoundNot FoundNot Found
um2NuXFnc0Od13BQqg 0.551.2e-100.776.5e-06
ldWImLHEDmUk16IL30Pcaf * 18519*0.551.5e-100.481.8e-04 ENSMUST00000000724 2 1 1 1 1 1-0.5192Not FoundNot Found
cuzncl0lPHj0yUv0XwCcnccyclin C518130.401.6e-100.931.2e-09transcription(GO:0006350) | regulation of transcription, DNA-dependent(GO:0006355) OTTMUST00000010608 1 2 1 1 1 1-0.4342Not FoundNot Found
c0rHdgr7fnpXuuvlDoPlekhg3pleckstrin homology domain containing, family G (with RhoGef domain) member 32634060.621.0e-090.481.1e-04intracellular signaling cascade(GO:0007242) | regulation of Rho protein signal transduction(GO:0035023) ENSMUST00000101279 1 0 0 0 0 0Not FoundNot FoundNot Found
Hg.3.j_oj70SgstdV0Adam19 * 11492*0.651.6e-090.581.2e-04 OTTMUST00000012216 3 1 3 1 3 1-0.3185-0.0716Not Found
WfXy3tUW5N58XTpnv4Rnf38ring finger protein 38734690.622.2e-090.943.2e-05 OTTMUST00000015974 2 3 3 2 1 1-0.3052Not FoundNot Found
0Xgh4_noK_vkoCFolUAcbd4acyl-Coenzyme A binding domain containing 4671310.433.9e-090.466.7e-05biological_process(GO:0008150) OTTMUST00000006883 0 1 1 1 0 0Not Found-0.20640.0318833
KrnNSu8OCCeiikq5ag4930519N13RikRIKEN cDNA 4930519N13 gene781770.454.8e-090.401.2e-04 OTTMUST00000037530 0 1 1 1 0 0Not Found-0.20480.00937581
ZZw4u1Ikd1fin5rUKILeprotleptin receptor overlapping transcript2305140.474.9e-090.879.3e-07 OTTMUST00000019180 0 2 1 1 0 0Not FoundNot Found0.0246051
ohJKUK40unAvgCq_v0Ric8bresistance to inhibitors of cholinesterase 8 homolog B (C. elegans)2374220.476.7e-090.701.7e-05 ENSMUST00000038523 0 1 1 1 0 0Not Found-0.1647Not Found
W3K5fkivgpSUZR59RQ 0.387.4e-090.511.2e-05
E8S57gidW_K_urHtf4 imb8_k2n0aNqiOX2H8Necap1NECAP endocytosis associated 1676020.671.1e-081.276.4e-07transport(GO:0006810) | endocytosis(GO:0006897) | protein transport(GO:0015031) ENSMUST00000032477 1 1 1 1 1 1-0.4297Not FoundNot Found
9nJyRQvr4SPv_45_V4Wwp2WW domain containing E3 ubiquitin protein ligase 2668940.311.5e-080.622.0e-04protein modification process(GO:0006464)Ubiquitin mediated proteolysis(04120) | Dentatorubropallidoluysian atrophy (DRPLA)(05050) -0.4071Not FoundNot Found
6B6Xp7uTy0O1T6.53sD16Ertd472eDNA segment, Chr 16, ERATO Doi 472, expressed671020.468.0e-080.704.5e-06 ENSMUST00000023571 2 1 0 0 0 0Not FoundNot FoundNot Found
oronqodncvXoVAxvxELmbr1llimb region 1 like747750.292.7e-070.752.3e-06endocytosis(GO:0006897) OTTMUST00000058993 2 1 1 1 1 1-0.3165Not Found0.00104278
lW8eG7B8kk8i4bfS_sSnap29synaptosomal-associated protein 29674740.492.9e-070.661.1e-04transport(GO:0006810) | protein transport(GO:0015031)SNARE interactions in vesicular transport(04130)OTTMUST00000057883 1 1 1 1 0 0Not FoundNot FoundNot Found
HQhFkIdI9XSOFUmVf4 cSdHs4EaE_eHrDgud0Gnaqguanine nucleotide binding protein, alpha q polypeptide146820.333.2e-070.982.6e-06skeletal development(GO:0001501) | regulation of action potential(GO:0001508) | signal transduction(GO:0007165) | protein amino acid ADP-ribosylation(GO:0006471) | G-protein coupled receptor protein signaling pathway(GO:0007186) | G-protein signaling, adenylate cyclase activating pathway(GO:0007189) | glutamate signaling pathway(GO:0007215) | heart development(GO:0007507) | behavior(GO:0007610) | post-embryonic development(GO:0009791) | neuron remodeling(GO:0016322) | forebrain neuron development(GO:0021884) | embryonic digit morphogenesis(GO:0042733) | regulation of melanocyte differentiation(GO:0045634) | pigmentation during development(GO:0048066) | dopamine receptor, phospholipase C activating pathway(GO:0060158)Calcium signaling pathway(04020) | Gap junction(04540) | Long-term potentiation(04720) | Long-term depression(04730) | GnRH signaling pathway(04912) | Melanogenesis(04916)ENSMUST00000025541 0 2 2 2 0 0Not FoundNot FoundNot Found
0uTv0tSkLxPa_7kMgoOsbpl2oxysterol binding protein-like 22289830.374.3e-070.644.3e-06transport(GO:0006810) | lipid transport(GO:0006869) | steroid metabolic process(GO:0008202) OTTMUST00000038983 1 1 1 1 1 1Not Found-0.23650.0127902
Bh9F3khPV5KzHUS_UABrms1l * 52592*0.359.5e-070.584.6e-05 ENSMUST00000012220 0 1 1 1 0 0-0.1796Not FoundNot Found
lenfuu6HT5eEyFcn1QGli2GLI-Kruppel family member GLI2146330.681.5e-060.476.3e-05negative regulation of transcription from RNA polymerase II promoter(GO:0000122) | skeletal development(GO:0001501) | kidney development(GO:0001822) | osteoblast development(GO:0002076) | pattern specification process(GO:0007389) | axon guidance(GO:0007411) | ventral midline development(GO:0007418) | hindgut morphogenesis(GO:0007442) | heart development(GO:0007507) | positive regulation of cell proliferation(GO:0008284) | dorsal/ventral pattern formation(GO:0009953) | proximal/distal pattern formation(GO:0009954) | floor plate formation(GO:0021508) | spinal cord dorsal/ventral patterning(GO:0021513) | ventral spinal cord development(GO:0021517) | cerebellar cortex morphogenesis(GO:0021696) | smoothened signaling pathway involved in ventral spinal cord interneuron specification(GO:0021775) | smoothened signaling pathway involved in spinal cord motor neuron cell fate specification(GO:0021776) | dorsoventral neural tube patterning(GO:0021904) | smoothened signaling pathway in regulation of granule cell precursor cell proliferation(GO:0021938) | spinal cord ventral commissure morphogenesis(GO:0021965) | pituitary gland development(GO:0021983) | cell differentiation(GO:0030154) | lung development(GO:0030324) | mammary gland development(GO:0030879) | hindbrain development(GO:0030902) | tube development(GO:0035295) | odontogenesis of dentine-containing tooth(GO:0042475) | positive regulation of neuron differentiation(GO:0045666) | positive regulation of transcription(GO:0045941) | positive regulation of transcription from RNA polymerase II promoter(GO:0045944) | embryonic gut development(GO:0048566) | developmental growth(GO:0048589) | anatomical structure formation(GO:0048646) | neuron development(GO:0048666) | branching morphogenesis of a tube(GO:0048754) | anatomical structure development(GO:0048856) | notochord regression(GO:0060032)Hedgehog signaling pathway(04340) | Basal cell carcinoma(05217)ENSMUST00000062483 0 0 0 0 0 0Not FoundNot FoundNot Found
fuf5P1KK.Vzv6sA78kZnrf2zinc and ring finger 23875240.293.3e-060.906.2e-05biological_process(GO:0008150) OTTMUST00000056945 1 1 1 1 1 1Not Found-0.2721Not Found
HLkRZF_ztElS698r3sTtc39btetratricopeptide repeat domain 39B698630.516.4e-060.681.8e-04 OTTMUST00000000091 2 3 3 1 2 0Not FoundNot FoundNot Found
TbIXcNVESkpfvK1MuIDbtdihydrolipoamide branched chain transacylase E2131710.242.2e-050.752.4e-06metabolic process(GO:0008152)Valine, leucine and isoleucine degradation(00280)OTTMUST00000066974 0 1 1 1 0 0Not Found-0.23650.000120217
ECyrniJ06TKt568y6IDcbld1discoidin, CUB and LCCL domain containing 1666860.455.2e-051.044.5e-05 Not FoundNot Found0.00284702
xQMgji9qDrurjHh1KkSfxn3sideroflexin 3942800.496.6e-050.457.6e-05transport(GO:0006810) | ion transport(GO:0006811) | cation transport(GO:0006812) | iron ion transport(GO:0006826) ENSMUST00000062213 0 0 2 0 0 0Not FoundNot FoundNot Found
fi_CR.P_vu.jRvefoIRgmaRGM domain family, member A2440580.267.3e-050.413.4e-05neural tube closure(GO:0001843) | positive regulation of protein amino acid phosphorylation(GO:0001934) | regulation of BMP signaling pathway(GO:0030510) OTTMUST00000077776 0 0 0 0 0 0Not FoundNot FoundNot Found
Eeuq_mu5hHURiKdEM8Ndufa5NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5682020.248.0e-051.407.4e-08respiratory electron transport chain(GO:0022904) | transport(GO:0006810)Oxidative phosphorylation(00190)OTTMUST00000066417 0 1 1 1 0 0Not Found-0.31310.00396649
6X9TfVN8x7_f_OKfqo 0.421.1e-040.505.6e-06
ry1e1qOt947dcQ5riQPpm1b * 19043*0.224.3e-040.601.4e-04 ENSMUST00000112304 1 0 0 0 0 0Not FoundNot FoundNot Found
oUuo767U67hPAhSgl4Hus1Hus1 homolog (S. pombe)155740.224.6e-040.876.2e-07DNA damage checkpoint(GO:0000077) | double-strand break repair via homologous recombination(GO:0000724) | DNA repair(GO:0006281) | protein amino acid phosphorylation(GO:0006468) | response to DNA damage stimulus(GO:0006974) | embryonic development(GO:0009790) | negative regulation of DNA replication(GO:0008156) | response to UV(GO:0009411) | G1/S transition checkpoint(GO:0031575) OTTMUST00000011503 1 0 2 0 1 0Not FoundNot FoundNot Found
ld.9cvPeXlLBVKCLckAsah1N-acylsphingosine amidohydrolase 1118860.225.7e-040.601.4e-05 Sphingolipid metabolism(00600)OTTMUST00000054076 1 1 1 1 0 0Not Found-0.13420.0236953
3zeTwolFuqW9i4VkjsPolspolymerase (DNA directed) sigma2101060.221.4e-030.604.5e-05DNA replication(GO:0006260) | cell cycle(GO:0007049) | mitosis(GO:0007067) | cell division(GO:0051301) OTTMUST00000081019 2 1 1 1 1 1-0.4701Not FoundNot Found
9EKV3r68p3OAfM.lf0 0.171.7e-030.758.5e-05
re7f7od_kU_3ejq6mAPrkar1aprotein kinase, cAMP dependent regulatory, type I, alpha190840.162.9e-030.997.6e-09mesoderm formation(GO:0001707) | regulation of protein amino acid phosphorylation(GO:0001932) | signal transduction(GO:0007165) | protein amino acid phosphorylation(GO:0006468) | organ morphogenesis(GO:0009887) | cell proliferation(GO:0008283)Apoptosis(04210) | Insulin signaling pathway(04910)OTTMUST00000007044 0 2 1 1 0 0Not Found-0.2854Not Found
Kl70hUQdU3V_lEg7HwSpryd4SPRY domain containing 4667010.175.0e-031.257.7e-10 ENSMUST00000061995 1 1 1 1 1 1-0.4078Not FoundNot Found
TJDtEB5LLt2heVnRScFbxw7 * 50754*0.286.4e-030.752.6e-06 OTTMUST00000054321 1 3 2 2 1 1-0.5395Not FoundNot Found