The files in this directory contain off-target counts for sets of crispr sites from the WGE database, http://www.sanger.ac.uk/htgt/wge/.

All crispr sites overlapping an exon:
crisprs_human_grch38_exonic_off_target_counts.csv.gz
crisprs_mouse_grcm38_exonic_off_target_counts.csv.gz
crisprs_human_grch38_exonic_off_target_lists.csv.gz
crisprs_mouse_grcm38_exonic_off_target_lists.csv.gz

The columns are as follows:
id - unique crispr ID assigned to this site by WGE
chr_name - name of the chromosome the crispr site is located on
chr_start - start position of the crispr site (note that all crispr sites in WGE are stored on the positive strand)
seq - crispr site sequence including the PAM site (again note that this is the sequence on the positive strand)
pam_right - boolean flag indicating at which end of the crispr site the PAM is
off_target_summary - a string containing the number of off-targets identified for this crispr with 0,1,2,3 and 4 mismatches, see: http://www.sanger.ac.uk/htgt/wge/crispr_help#summary_explanation
off_target_ids - the WGE IDs  for all crispr sites identified as off targets for this one with up to 4 mismatches (only included in the *_off_target_lists.csv.gz file)
exonic - boolean flag indicating if the crispr site overlaps an exon
genic - boolean flag indicating if the crispr site overlaps a gene

Please contact wge@sanger.ac.uk for support.