##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	8016-7-1.h.sapiens.aligned.bam	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	10142212	
Filtered Sequences	0	
Sequence length	1-100	
%GC	46	
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.818505962998998	24.0	24.0	32.0	24.0	32.0
2	31.007281905194237	31.0	31.0	32.0	30.0	32.0
3	32.69452565414053	34.0	33.0	34.0	30.0	34.0
4	34.2768169392509	35.0	35.0	35.0	29.0	37.0
5	35.39334135862803	36.0	36.0	36.0	34.0	36.0
6	35.57256770084956	36.0	36.0	37.0	34.0	37.0
7	35.9253854445292	37.0	35.0	37.0	33.0	38.0
8	36.265933705342135	37.0	36.0	37.0	34.0	38.0
9	36.506527475899354	37.0	37.0	38.0	35.0	38.0
10-14	36.77844663838828	37.6	36.8	38.0	34.4	38.2
15-19	37.27172657475408	38.0	37.0	38.8	34.6	39.2
20-24	36.89219836185616	37.8	36.6	38.2	35.2	38.6
25-29	37.02228885966191	38.0	36.8	38.6	34.2	39.0
30-34	37.0096976644155	37.8	36.8	38.8	35.4	39.2
35-39	36.923063687205804	38.0	37.2	38.6	33.6	39.2
40-44	36.79360875394428	38.0	36.8	38.2	33.6	39.2
45-49	36.659923196330475	37.4	37.0	38.0	33.8	39.2
50-54	36.88972774300127	38.2	37.2	38.2	34.0	39.0
55-59	36.95897492396354	38.0	37.2	38.4	33.0	38.8
60-64	36.43820725912616	37.4	36.4	38.0	33.4	38.4
65-69	36.534632131637	37.8	36.4	38.4	34.0	38.8
70-74	36.57129578401596	37.6	36.8	38.6	34.2	38.6
75-79	36.23728118642532	37.2	36.8	37.8	34.2	38.6
80-84	36.48312629698025	37.8	36.4	38.0	33.6	38.8
85-89	36.42095854651711	38.0	36.2	38.6	33.0	39.2
90-94	36.233628982034865	37.6	36.4	38.4	33.2	39.6
95-99	35.92424703791913	37.2	36.4	38.0	32.4	38.6
100	34.930437214302394	37.0	35.0	37.0	32.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	17.0
6	122.0
7	257.0
8	451.0
9	660.0
10	873.0
11	1236.0
12	1504.0
13	2047.0
14	2465.0
15	2937.0
16	3744.0
17	4653.0
18	6565.0
19	6894.0
20	6568.0
21	7616.0
22	9285.0
23	11451.0
24	14944.0
25	20335.0
26	27571.0
27	37954.0
28	50914.0
29	68461.0
30	89884.0
31	118979.0
32	160320.0
33	219158.0
34	328694.0
35	635898.0
36	2128506.0
37	6167948.0
38	3301.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.667275418616015	18.05794680708041	21.599028785007718	38.67574898929586
2	20.580592484779636	45.995905737292695	17.124687654693513	16.298814123234152
3	18.933356892598642	33.696999910962546	29.743391680312335	17.62625151612648
4	34.99881283682304	23.17097003458155	20.241595594638596	21.58862153395681
5	22.17035297142937	26.049092709353644	24.928805632524067	26.85174868669292
6	22.301105858702428	28.160419617736572	24.748363259310327	24.790111264250672
7	23.238122676933635	26.684964765953822	26.02611290019944	24.050799656913103
8	23.057516942557992	25.951289677414014	27.136075668542315	23.85511771148568
9	22.467887143853844	27.99673320289283	25.360361284175063	24.175018369078266
10-14	22.96009053983118	26.538605547219163	26.20417878844033	24.297125124509325
15-19	22.357029499587004	26.84609408563591	26.673114459057086	24.12376195572
20-24	22.599975776321845	25.850788651129864	27.11375308695592	24.435482485592363
25-29	22.77929140051057	26.25653515909231	27.32751421374483	23.636659226652288
30-34	22.743154075981415	26.96361823688409	26.83550579788912	23.457721889245374
35-39	23.153120828984935	26.06037688097408	27.710884411300047	23.07561787874094
40-44	23.075526757927342	26.583045026890638	26.901613923696722	23.439814291485295
45-49	23.025546202528638	27.29611106036389	26.942287775311907	22.736054961795563
50-54	23.167690245316322	26.874917437792494	26.728908218419296	23.22848409847189
55-59	23.099580083434347	27.08541409014822	26.774380105027078	23.040625721390356
60-64	23.113655828579976	26.794045561933526	27.447041707372666	22.645256902113832
65-69	23.185041819885384	27.40096580690467	27.221501823373867	22.192490549836073
70-74	23.07572734502373	27.48776690409549	26.71282862263034	22.72367712825044
75-79	23.399678264196904	27.38432840621944	26.763906419120175	22.45208691046348
80-84	22.582685188194375	27.59985927545714	26.729356172455443	23.08809936389304
85-89	22.960534367477692	27.481861464808706	26.97468478040625	22.582919387307353
90-94	22.936798676994375	26.607206521743414	27.349816634238206	23.106178167024
95-99	23.02442127229191	26.54771321919266	27.13726113888388	23.29060436963156
100	24.434087136298036	25.689077501296147	26.069266292870953	23.807569069534868
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	60.34302440791187
2	36.87940660801379
3	36.55960840872512
4	56.58743437077985
5	49.02210165812229
6	47.0912171229531
7	47.28892233384674
8	46.91263465404367
9	46.64290551293211
10-14	47.25721566434051
15-19	46.480791455307
20-24	47.03545826191421
25-29	46.41595062716286
30-34	46.20087596522679
35-39	46.228738707725874
40-44	46.51534104941264
45-49	45.7616011643242
50-54	46.39617434378821
55-59	46.1402058048247
60-64	45.758912730693815
65-69	45.37753236972146
70-74	45.799404473274166
75-79	45.851765174660386
80-84	45.67078455208742
85-89	45.54345375478505
90-94	46.042976844018376
95-99	46.31502564192347
100	48.2416562058329
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	88.0
1	88.0
2	91.5
3	93.5
4	95.0
5	107.5
6	123.5
7	140.5
8	169.0
9	202.0
10	246.0
11	286.5
12	323.5
13	421.0
14	463.0
15	662.5
16	837.5
17	855.0
18	1402.5
19	1858.0
20	3293.0
21	4919.5
22	8539.0
23	14826.0
24	26527.5
25	42646.5
26	56044.5
27	70670.0
28	87043.0
29	124000.0
30	171112.5
31	179942.5
32	184373.0
33	212995.0
34	266402.5
35	287229.5
36	267630.5
37	255965.0
38	285267.5
39	322997.0
40	348460.5
41	359937.0
42	335042.5
43	358104.5
44	399398.0
45	388744.5
46	351023.0
47	316196.0
48	323461.0
49	349089.5
50	362272.0
51	365996.5
52	306273.5
53	244614.0
54	233541.0
55	203615.0
56	169708.0
57	150387.0
58	186561.5
59	198288.0
60	180332.5
61	197800.0
62	177806.5
63	146187.5
64	113530.5
65	105946.0
66	134872.5
67	123771.0
68	74949.5
69	55462.0
70	46296.5
71	38185.0
72	35542.5
73	21459.5
74	13185.0
75	16999.5
76	17630.5
77	14636.5
78	11014.5
79	9797.5
80	8484.0
81	8743.0
82	6274.0
83	3106.5
84	2862.5
85	1080.0
86	3073.5
87	2807.0
88	427.0
89	317.5
90	240.0
91	194.5
92	144.5
93	101.5
94	76.0
95	75.0
96	61.5
97	49.5
98	44.5
99	38.0
100	32.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03854188810093893
2	0.01090501143448622
3	0.003214316526368981
4	0.0029678198602363904
5	0.0014296843286581997
6	0.0027509147284467557
7	1.8733874078391994E-4
8	0.0013606711224373289
9	7.887950091362182E-5
10-14	3.8848158796443813E-4
15-19	0.012332894359333647
20-24	0.01207487963575395
25-29	0.00842287859715192
30-34	0.009795583893614635
35-39	0.006348385554671698
40-44	0.01043102148317095
45-49	0.010111820921801249
50-54	0.010200451017655576
55-59	0.010184701075443576
60-64	0.039134727481904705
65-69	0.03021967370425955
70-74	0.018735305104931533
75-79	0.03945152407547536
80-84	0.04537753379841414
85-89	0.012387997029610166
90-94	0.0025259127096040556
95-99	0.00647655665343735
100	0.012364591440478107
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
0-1	86.0
2-3	1.0
4-5	44.0
6-7	27.0
8-9	2.0
10-11	2.0
12-13	0.0
14-15	62.0
16-17	4.0
18-19	37.0
20-21	304.0
22-23	53.0
24-25	146.0
26-27	40.0
28-29	47.0
30-31	54.0
32-33	46.0
34-35	55.0
36-37	75.0
38-39	117.0
40-41	105.0
42-43	116.0
44-45	141.0
46-47	93.0
48-49	154.0
50-51	28491.0
52-53	766.0
54-55	28451.0
56-57	37687.0
58-59	70893.0
60-61	1341.0
62-63	47358.0
64-65	14157.0
66-67	14748.0
68-69	19045.0
70-71	41621.0
72-73	37844.0
74-75	24198.0
76-77	19378.0
78-79	13145.0
80-81	20435.0
82-83	27140.0
84-85	15596.0
86-87	8422.0
88-89	12670.0
90-91	30773.0
92-93	6763.0
94-95	10790.0
96-97	243236.0
98-99	0.0
100-101	9365453.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	98.48310147268415
#Duplication Level	Relative count
1	100.0
2	13.52773928262383
3	4.865353710824404
4	2.240743270938653
5	1.1958438246182475
6	0.675996590739042
7	0.4651949589782627
8	0.33572111724864834
9	0.2608995504199767
10++	6.556971743472632
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CATGCACTATCCAGGCGCCTTCACCTACTCCCCGACGCCGGTCACCTCGGGCATCGGCATCGG	15953	0.15729310331907873	No Hit
CCGATGCCGATGCCCGAGGTGACCGGCGTCGGGGAGTAGGTGAAGGCGCCTGGATAGTGCATG	15313	0.1509828427960291	No Hit
CATGCTTTCCCTGGGTCTTCACGGATTGCTTTCCAAGCTGCCTTGTTGCG	13985	0.13788905221070116	No Hit
GCGACAGCTGGAAAGGTCCAGTTGCTGCTCTGCAGCAACCCCGCAACAAG	13910	0.1371495685556563	No Hit
AGGACAACAGCTGATCCATACATTTGCTGACAGGTGTACCTGCGTTTCCC	13739	0.13546354582215397	No Hit
CTTGGGGGAGTGTGCCTCCAGCAGAGAAGTAATTCTGGCCTGGCAACCTA	13686	0.13494097737258892	No Hit
CATGGGGTGGGTGCTTGCAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTT	13016	0.12833492338752137	No Hit
GGCTGCTTGTTGTACGTGGCCTGGTGGAACGCACTGCAAAACGAGCTCAG	12933	0.12751656147593837	No Hit
CATGTAGACTTACCTTTCCTCATAGAGCTATCCTGGTTAATAACAGGCCA	12643	0.12465722467643155	No Hit
ACACTTACACCAAGGAATCTCCCAGGCATTATCAAAGCTACAGGAGACAA	12584	0.1240754975344629	No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA	12573	0.12396703993172299	No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG	12570	0.12393746058552119	No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC	12444	0.1226951280450458	No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG	12418	0.12243877371129691	No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT	11835	0.11669052076608141	No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT	11817	0.11651304468887062	No Hit
CATGGGGTTGTGCCAATTAATTACCAAACATTGAGCCTGCAGGCTTTGAG	11709	0.115448188225606	No Hit
AAGACAGGGGAAAGGAAGAAAAGGGAGTGAAGAGGGAATGGGAGAGGGAA	11532	0.11370300679970011	No Hit
ATCCTTCACAAAGTCTTTGAGATATATTTTTATCAAATATTTAGCATGGATCCCGGTACACTTTCAATACTTA	11509	0.11347623181215301	No Hit
TAAGTATTGAAAGTGTACCGGGATCCATGCTAAATATTTGATAAAAATATATCTCAAAGACTTTGTGAAGGAT	11442	0.11281562641364624	No Hit
CATGTTTGGGCCCCTGTTCCCGGTCTTTGGTAAACAGACGCTTAAGTTGA	11318	0.11159301343730539	No Hit
AGTGACAAGTTGGGAATGATGTGGTGATTTAGAATGCAGTATTGGCCAAG	11256	0.11098170694913496	No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG	10963	0.10809279080342632	No Hit
CATGGCTCTGCTGTGCCTTCCATCCTGGGCTCCCTTCTCTCCTGTGACCT	10936	0.10782657668761016	No Hit
ACAGTGAAAACAGTGACAATGTTCCAGCAAAGCCACCAGACAAAGTTCTT	10930	0.10776741799520656	No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA	10719	0.10568700397901366	No Hit
CATGGAGGGGAGCACTCAGCACCTCCCTCACCCCACACCCTTGGCTGCTC	10576	0.10427705514339475	No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA	10550	0.10402070080964586	No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC	10534	0.10386294429656961	No Hit
CATGGAATATATATTCTCCTGGCTGGTGCTTATGATTGCAAAACTTGGAT	10527	0.10379392582209876	No Hit
GCCTCAGCCTTCCTTCGCAGAAAGTTGAGCAGGTCGCCATAGCAACAGTA	10520	0.10372490734762792	No Hit
CACACTGTCATTAGTTGCAGCATCTCCTGTAGCAATTGGGCTAAATCTGT	10474	0.10327135737253372	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	4070695	3.1362228	4.335498	65-69
AAAAA	4198285	3.0608711	5.9761105	2
CCCAG	2176015	2.89611	5.192953	2
GGAGG	1859390	2.573778	5.294524	4
CCCCA	1928060	2.5327437	6.1380296	1
GGGGG	1505860	2.446764	7.820716	4
CCTCC	1823285	2.4216888	5.7320366	6
TGGGG	1693095	2.3695998	10.706645	3
AGGAG	1931405	2.2775457	5.0879636	6
CTCCC	1691970	2.2472758	5.0621867	3
CCACC	1593010	2.0926144	5.6044765	1
GAGGG	1509330	2.089223	5.255016	8
CAGGG	1496775	2.044912	5.194056	2
TCCAG	1748975	2.0314465	5.154897	7
AGAAG	2019400	2.0286598	5.254102	3
TGGCT	1632280	1.9421912	6.5967894	3
TGCAG	1635060	1.9241458	6.876739	3
GCCCA	1394745	1.8562993	8.694463	2
GGCTC	1333540	1.8181709	9.387211	1
AGCCC	1335530	1.7774887	5.1935887	1
GCTCC	1202415	1.6180823	8.994652	2
TGCCC	1179920	1.5878109	9.533723	3
TAAAA	2128965	1.5694039	8.319625	1
CAGCT	1345700	1.56304	5.218418	8
GGCCC	991955	1.5497156	8.529229	1
CATGG	1289740	1.5177715	39.283318	1
ATGGA	1421255	1.4436167	11.142244	2
TCAGG	1216835	1.4319769	6.037225	1
ACAGC	1236930	1.420934	5.106443	7
TGGGT	1118060	1.3478606	6.231197	3
GCTCT	1139755	1.3385247	6.5670757	2
ATGGG	1115285	1.329758	13.000435	2
TGCTC	1126935	1.3234689	6.792179	1
ATGTG	1287950	1.3227323	10.683277	2
ATGGC	1089700	1.2823638	10.510105	2
GGCAC	934795	1.2605265	5.203466	1
ATGAA	1394695	1.2068472	8.272092	2
TGAGA	1182345	1.2009478	5.604775	1
ATGCC	951685	1.1053888	7.594842	2
CATGT	1072355	1.0869987	30.383753	1
ATGTT	1223705	1.0825198	7.177274	2
CATGA	1036040	1.0386612	23.86523	1
ATGCT	1015620	1.029489	9.168215	2
ATGGT	967840	0.9939774	6.726992	2
TAAAG	1125215	0.97366273	5.3891044	1
ATGAC	957215	0.9596368	6.262214	2
CATGC	818165	0.95030427	30.403576	1
ATGTA	1011500	0.8849769	7.4215508	2
ATGCA	866315	0.86850685	11.086757	2
TAAGT	862815	0.75489014	8.402019	1
TAACT	815450	0.7041756	5.3960023	1
TAAGG	665575	0.676047	5.2592983	1
>>END_MODULE