FastQCFastQC Report
Wed 20 Mar 2013
8016-7-1.h.sapiens.aligned.bam

Summary

[OK] Basic Statistics

Measure Value
Filename 8016-7-1.h.sapiens.aligned.bam
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 10142212
Filtered Sequences 0
Sequence length 1-100
%GC 46

[WARN] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
CATGCACTATCCAGGCGCCTTCACCTACTCCCCGACGCCGGTCACCTCGGGCATCGGCATCGG 15953 0.15729310331907873 No Hit
CCGATGCCGATGCCCGAGGTGACCGGCGTCGGGGAGTAGGTGAAGGCGCCTGGATAGTGCATG 15313 0.1509828427960291 No Hit
CATGCTTTCCCTGGGTCTTCACGGATTGCTTTCCAAGCTGCCTTGTTGCG 13985 0.13788905221070116 No Hit
GCGACAGCTGGAAAGGTCCAGTTGCTGCTCTGCAGCAACCCCGCAACAAG 13910 0.1371495685556563 No Hit
AGGACAACAGCTGATCCATACATTTGCTGACAGGTGTACCTGCGTTTCCC 13739 0.13546354582215397 No Hit
CTTGGGGGAGTGTGCCTCCAGCAGAGAAGTAATTCTGGCCTGGCAACCTA 13686 0.13494097737258892 No Hit
CATGGGGTGGGTGCTTGCAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTT 13016 0.12833492338752137 No Hit
GGCTGCTTGTTGTACGTGGCCTGGTGGAACGCACTGCAAAACGAGCTCAG 12933 0.12751656147593837 No Hit
CATGTAGACTTACCTTTCCTCATAGAGCTATCCTGGTTAATAACAGGCCA 12643 0.12465722467643155 No Hit
ACACTTACACCAAGGAATCTCCCAGGCATTATCAAAGCTACAGGAGACAA 12584 0.1240754975344629 No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA 12573 0.12396703993172299 No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG 12570 0.12393746058552119 No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC 12444 0.1226951280450458 No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG 12418 0.12243877371129691 No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT 11835 0.11669052076608141 No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT 11817 0.11651304468887062 No Hit
CATGGGGTTGTGCCAATTAATTACCAAACATTGAGCCTGCAGGCTTTGAG 11709 0.115448188225606 No Hit
AAGACAGGGGAAAGGAAGAAAAGGGAGTGAAGAGGGAATGGGAGAGGGAA 11532 0.11370300679970011 No Hit
ATCCTTCACAAAGTCTTTGAGATATATTTTTATCAAATATTTAGCATGGATCCCGGTACACTTTCAATACTTA 11509 0.11347623181215301 No Hit
TAAGTATTGAAAGTGTACCGGGATCCATGCTAAATATTTGATAAAAATATATCTCAAAGACTTTGTGAAGGAT 11442 0.11281562641364624 No Hit
CATGTTTGGGCCCCTGTTCCCGGTCTTTGGTAAACAGACGCTTAAGTTGA 11318 0.11159301343730539 No Hit
AGTGACAAGTTGGGAATGATGTGGTGATTTAGAATGCAGTATTGGCCAAG 11256 0.11098170694913496 No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG 10963 0.10809279080342632 No Hit
CATGGCTCTGCTGTGCCTTCCATCCTGGGCTCCCTTCTCTCCTGTGACCT 10936 0.10782657668761016 No Hit
ACAGTGAAAACAGTGACAATGTTCCAGCAAAGCCACCAGACAAAGTTCTT 10930 0.10776741799520656 No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA 10719 0.10568700397901366 No Hit
CATGGAGGGGAGCACTCAGCACCTCCCTCACCCCACACCCTTGGCTGCTC 10576 0.10427705514339475 No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA 10550 0.10402070080964586 No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC 10534 0.10386294429656961 No Hit
CATGGAATATATATTCTCCTGGCTGGTGCTTATGATTGCAAAACTTGGAT 10527 0.10379392582209876 No Hit
GCCTCAGCCTTCCTTCGCAGAAAGTTGAGCAGGTCGCCATAGCAACAGTA 10520 0.10372490734762792 No Hit
CACACTGTCATTAGTTGCAGCATCTCCTGTAGCAATTGGGCTAAATCTGT 10474 0.10327135737253372 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 4070695 3.1362228 4.335498 65-69
AAAAA 4198285 3.0608711 5.9761105 2
CCCAG 2176015 2.89611 5.192953 2
GGAGG 1859390 2.573778 5.294524 4
CCCCA 1928060 2.5327437 6.1380296 1
GGGGG 1505860 2.446764 7.820716 4
CCTCC 1823285 2.4216888 5.7320366 6
TGGGG 1693095 2.3695998 10.706645 3
AGGAG 1931405 2.2775457 5.0879636 6
CTCCC 1691970 2.2472758 5.0621867 3
CCACC 1593010 2.0926144 5.6044765 1
GAGGG 1509330 2.089223 5.255016 8
CAGGG 1496775 2.044912 5.194056 2
TCCAG 1748975 2.0314465 5.154897 7
AGAAG 2019400 2.0286598 5.254102 3
TGGCT 1632280 1.9421912 6.5967894 3
TGCAG 1635060 1.9241458 6.876739 3
GCCCA 1394745 1.8562993 8.694463 2
GGCTC 1333540 1.8181709 9.387211 1
AGCCC 1335530 1.7774887 5.1935887 1
GCTCC 1202415 1.6180823 8.994652 2
TGCCC 1179920 1.5878109 9.533723 3
TAAAA 2128965 1.5694039 8.319625 1
CAGCT 1345700 1.56304 5.218418 8
GGCCC 991955 1.5497156 8.529229 1
CATGG 1289740 1.5177715 39.283318 1
ATGGA 1421255 1.4436167 11.142244 2
TCAGG 1216835 1.4319769 6.037225 1
ACAGC 1236930 1.420934 5.106443 7
TGGGT 1118060 1.3478606 6.231197 3
GCTCT 1139755 1.3385247 6.5670757 2
ATGGG 1115285 1.329758 13.000435 2
TGCTC 1126935 1.3234689 6.792179 1
ATGTG 1287950 1.3227323 10.683277 2
ATGGC 1089700 1.2823638 10.510105 2
GGCAC 934795 1.2605265 5.203466 1
ATGAA 1394695 1.2068472 8.272092 2
TGAGA 1182345 1.2009478 5.604775 1
ATGCC 951685 1.1053888 7.594842 2
CATGT 1072355 1.0869987 30.383753 1
ATGTT 1223705 1.0825198 7.177274 2
CATGA 1036040 1.0386612 23.86523 1
ATGCT 1015620 1.029489 9.168215 2
ATGGT 967840 0.9939774 6.726992 2
TAAAG 1125215 0.97366273 5.3891044 1
ATGAC 957215 0.9596368 6.262214 2
CATGC 818165 0.95030427 30.403576 1
ATGTA 1011500 0.8849769 7.4215508 2
ATGCA 866315 0.86850685 11.086757 2
TAAGT 862815 0.75489014 8.402019 1
TAACT 815450 0.7041756 5.3960023 1
TAAGG 665575 0.676047 5.2592983 1
Produced by FastQC (version 0.10.0)