##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	8016-7-10.h.sapiens.aligned.bam	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	9053076	
Filtered Sequences	0	
Sequence length	1-100	
%GC	45	
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.851539741851276	24.0	24.0	32.0	24.0	32.0
2	31.024918501825493	31.0	31.0	32.0	30.0	32.0
3	32.72745585400476	34.0	33.0	34.0	30.0	34.0
4	34.2905990157252	35.0	35.0	35.0	29.0	37.0
5	35.41138030662188	36.0	36.0	36.0	34.0	36.0
6	35.5934619724353	36.0	36.0	37.0	34.0	37.0
7	35.9471005381415	37.0	35.0	37.0	33.0	38.0
8	36.29157790430235	37.0	36.0	37.0	34.0	38.0
9	36.52941741165574	37.0	37.0	38.0	35.0	38.0
10-14	36.80196309435709	37.6	36.8	38.0	34.8	38.2
15-19	37.30561659562348	38.0	37.0	38.8	34.8	39.2
20-24	36.9322601346152	37.8	37.0	38.2	35.6	38.6
25-29	37.06543873183561	38.0	37.0	38.6	34.2	39.0
30-34	37.06489759866572	37.8	36.8	38.8	35.4	39.2
35-39	36.97855736229971	38.0	37.2	38.6	33.8	39.2
40-44	36.85686986180396	38.0	36.8	38.2	33.6	39.2
45-49	36.71575428393357	37.4	37.0	38.4	33.8	39.2
50-54	36.9653972623082	38.2	37.2	38.2	34.6	39.0
55-59	37.02334556350196	38.0	37.2	38.4	34.0	38.8
60-64	36.50839233910567	37.4	36.4	38.0	34.0	38.4
65-69	36.60955080110418	37.8	36.4	38.4	34.2	38.8
70-74	36.65770914724173	37.6	36.8	38.6	34.2	38.6
75-79	36.32201529220955	37.2	36.8	37.8	34.4	38.6
80-84	36.56920807631617	37.8	36.4	38.0	34.4	38.8
85-89	36.50266656274995	38.0	36.2	38.6	33.0	39.2
90-94	36.303737630253394	37.6	36.4	38.4	33.2	39.6
95-99	35.99727129106621	37.2	36.4	38.0	32.6	38.6
100	34.99034124896572	37.0	35.0	37.0	33.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
1	14.0
2	0.0
3	43.0
4	6.0
5	15.0
6	94.0
7	244.0
8	290.0
9	1078.0
10	689.0
11	937.0
12	1213.0
13	1618.0
14	2018.0
15	2484.0
16	2850.0
17	3597.0
18	4765.0
19	4895.0
20	4923.0
21	6057.0
22	7192.0
23	8884.0
24	12007.0
25	16107.0
26	22455.0
27	31001.0
28	42098.0
29	56060.0
30	75321.0
31	101528.0
32	135899.0
33	188098.0
34	285248.0
35	553267.0
36	1865463.0
37	5611719.0
38	2899.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.236234687127517	16.283125168663442	20.818743462046886	40.66189668216215
2	19.047882623372313	47.58755110568135	16.927699781977083	16.436866488969258
3	21.42179240331588	32.0127935222851	29.481220947978702	17.08419312642032
4	34.15314168298429	23.507737176282788	20.4783102216895	21.860810919043423
5	22.386936036205128	26.300548629123156	25.08867734790187	26.223837986769844
6	22.18069213111563	27.97490209698974	25.425750637616517	24.41865513427811
7	22.895735894520065	26.341460289152035	26.41494468454404	24.347859131783856
8	22.621718394489516	25.99542383643883	27.45371141623778	23.92914635283388
9	22.795988402889666	28.080815222284393	25.915951329254423	23.20724504557152
10-14	23.251171222301984	26.271138788674563	25.8875749874909	24.590115001532553
15-19	22.38269732626697	26.500527307925932	26.80341973337467	24.313355632432422
20-24	22.660186403473155	25.58014772360668	26.812559655566304	24.947106217353863
25-29	22.555373409598612	26.40256254300331	27.70939679986217	23.332667247535912
30-34	22.56211836357679	27.333463493188464	26.949505917816385	23.154912225418364
35-39	22.771220787206918	26.6816471838465	27.81017633483467	22.736955694111906
40-44	22.469971409446543	27.299839879197147	27.293887704139102	22.936301007217207
45-49	22.76588399759214	27.567214641557353	27.440084573015067	22.22681678783544
50-54	22.776712302376826	27.30080573855382	27.368558459391217	22.553923499678145
55-59	22.49851227840661	27.813443367962726	27.26731757867225	22.420726774958414
60-64	22.47742855552409	27.33923555040059	27.94349246893751	22.239843425137813
65-69	22.739954719883027	27.854609604631957	27.727038203492448	21.67839747199257
70-74	22.373968437863088	27.883981874353054	27.29131144050238	22.45073824728148
75-79	22.768355705180507	27.933164114247035	27.202542673774634	22.09593750679783
80-84	22.301386244027952	27.699209073649396	27.3709335670092	22.62847111531345
85-89	22.640194295753318	27.789375234253793	27.311468485539798	22.25896198445309
90-94	22.50312860634859	27.23360794665081	27.601406610984085	22.66185683601651
95-99	22.61399865660665	26.846911246737115	27.63514347556021	22.903946621096026
100	23.370059284466688	26.385176005499638	26.741704360053873	23.5030603499798
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	62.898131369289665
2	35.484749112341575
3	38.5059855297362
4	56.013952602027715
5	48.610774022974965
6	46.599347265393746
7	47.24359502630392
8	46.55086474732339
9	46.00323344846118
10-14	47.84128622383454
15-19	46.6960529586994
20-24	47.60729262082702
25-29	45.888040657134525
30-34	45.71703058899515
35-39	45.50817648131883
40-44	45.40627241666375
45-49	44.99270078542758
50-54	45.33063580205497
55-59	44.919239053365025
60-64	44.717271980661906
65-69	44.4183521918756
70-74	44.82470668514457
75-79	44.864293211978335
80-84	44.929857359341405
85-89	44.89915628020641
90-94	45.1649854423651
95-99	45.51794527770268
100	46.87311963444649
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	710.0
1	710.0
2	710.5
3	712.0
4	720.5
5	730.0
6	732.0
7	740.5
8	770.0
9	807.0
10	842.0
11	876.0
12	918.0
13	977.5
14	1037.0
15	1275.5
16	1489.5
17	1680.5
18	2154.0
19	2940.0
20	4359.5
21	5666.5
22	8587.5
23	14204.0
24	27331.5
25	41194.5
26	54607.0
27	69010.0
28	78495.5
29	98824.5
30	130906.5
31	152093.0
32	169296.5
33	193883.5
34	229794.5
35	246471.0
36	232778.0
37	230569.0
38	261907.5
39	309258.5
40	349668.5
41	347892.5
42	325554.5
43	360226.5
44	397301.0
45	386830.0
46	342763.5
47	282798.0
48	280312.5
49	309767.0
50	327791.5
51	352673.5
52	300273.5
53	214414.0
54	196048.5
55	179920.0
56	141045.5
57	116049.5
58	145636.0
59	155851.0
60	139947.5
61	148529.0
62	128546.5
63	103157.5
64	86714.0
65	82118.0
66	107169.5
67	106023.5
68	66155.0
69	44890.5
70	36819.5
71	31551.5
72	28411.0
73	17174.5
74	10948.0
75	13418.0
76	13836.5
77	12450.5
78	8636.0
79	7016.0
80	6694.5
81	6238.5
82	4300.5
83	3164.5
84	2858.0
85	1616.5
86	3243.0
87	2377.5
88	575.0
89	417.0
90	297.0
91	233.0
92	145.5
93	103.0
94	80.5
95	79.5
96	69.5
97	59.5
98	55.5
99	48.0
100	45.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03846206526930736
2	0.011135230078377438
3	0.002971604058614614
4	0.0030378851900335273
5	0.0013587673969176085
6	0.0025518370907201284
7	4.418783098066274E-5
8	0.0015023872491510407
9	3.314090252398904E-5
10-14	3.4024725326014865E-4
15-19	0.012518501853040964
20-24	0.01235580611857663
25-29	0.008666762070289253
30-34	0.01013888728673255
35-39	0.00665411055967125
40-44	0.011133946973373093
45-49	0.010491395806725902
50-54	0.010475031728984027
55-59	0.010241804889983734
60-64	0.03941281955784908
65-69	0.03086224734173418
70-74	0.019129872203668852
75-79	0.040226958917458075
80-84	0.04636934134760823
85-89	0.012799787332322437
90-94	0.0025981797655436106
95-99	0.0068763318472960236
100	0.012429242269930665
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
0-1	726.0
2-3	0.0
4-5	48.0
6-7	42.0
8-9	2.0
10-11	21.0
12-13	6.0
14-15	20.0
16-17	10.0
18-19	52.0
20-21	697.0
22-23	182.0
24-25	537.0
26-27	137.0
28-29	139.0
30-31	214.0
32-33	61.0
34-35	101.0
36-37	71.0
38-39	105.0
40-41	104.0
42-43	117.0
44-45	119.0
46-47	203.0
48-49	854.0
50-51	20747.0
52-53	748.0
54-55	24678.0
56-57	41571.0
58-59	79416.0
60-61	4259.0
62-63	35995.0
64-65	20623.0
66-67	17568.0
68-69	23245.0
70-71	34752.0
72-73	39407.0
74-75	17626.0
76-77	14042.0
78-79	4196.0
80-81	15374.0
82-83	19763.0
84-85	10946.0
86-87	6921.0
88-89	9390.0
90-91	25814.0
92-93	2855.0
94-95	9421.0
96-97	193741.0
98-99	0.0
100-101	8375410.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	98.5707950061167
#Duplication Level	Relative count
1	100.0
2	12.899286636215384
3	4.9286249777798385
4	2.575220076205492
5	1.4901033334106208
6	0.8872606985245813
7	0.6090256362694861
8	0.35474970437524633
9	0.2828723132926801
10++	7.5378515615942865
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CATGCACTATCCAGGCGCCTTCACCTACTCCCCGACGCCGGTCACCTCGGGCATCGGCATCGG	14726	0.16266294461683523	No Hit
CCGATGCCGATGCCCGAGGTGACCGGCGTCGGGGAGTAGGTGAAGGCGCCTGGATAGTGCATG	14247	0.1573719252992022	No Hit
GACAACTTGGCCACCGACAGCTGAAGGGCTTTTCACCTGTTGACACAATTGCCAGTCA	13331	0.1472538173765469	No Hit
TGACTGGCAATTGTGTCAACAGGTGAAAAGCCCTTCAGCTGTCGGTGGCCAAGTTGTC	13282	0.14671256487850096	No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG	12513	0.13821821444998364	No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA	12384	0.1367932844041075	No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG	11415	0.12608974010601479	No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC	11380	0.1257031311788391	No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT	11280	0.12459853424405141	No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT	11235	0.12410146562339697	No Hit
ATTCAGCTGCCAAGTAACTTAGCACACCCATTCTTATAAGATAATGGCAA	11130	0.12294163884186987	No Hit
TAATGAGCATGAAGTCACCACACGGAGGAAAATGTAAATGTGTAAACCTC	11077	0.1223562024664324	No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA	10806	0.11936274477315775	No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC	10735	0.11857848094945851	No Hit
GAGCTACCATCTGATCTGTCTGTCTTGACCACCCGGAGTCCCACTGTCCCCAGCCAGAATCCCAGTAGACTA	10621	0.11731924044380054	No Hit
TAGTCTACTGGGATTCTGGCTGGGGACAGTGGGACTCCGGGTGGTCAAGACAGACAGATCAGATGGTAGCTC	10467	0.11561816116422749	No Hit
CATGTAATTCAGGCAGTTATGGGAGCCCTAGAGGGCCTGAGAGTTGCTATTGGAC	9960	0.11001785470485391	No Hit
GTCCAATAGCAACTCTCAGGCCCTCTAGGGCTCCCATAACTGCCTGAATTACATG	9915	0.10952078608419945	No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA	9759	0.10779761486593065	No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC	9686	0.10699125910353564	No Hit
CAACACCCAACACTTATAAGCGGAAGAACACAGAAACAGCTCTAGACAAC	9487	0.10479311120330814	No Hit
CATGCTTTCCCTGGGTCTTCACGGATTGCTTTCCAAGCTGCCTTGTTGCG	9465	0.10455009987765485	No Hit
GCGACAGCTGGAAAGGTCCAGTTGCTGCTCTGCAGCAACCCCGCAACAAG	9388	0.10369956023786832	No Hit
CATGGAATATATATTCTCCTGGCTGGTGCTTATGATTGCAAAACTTGGAT	9378	0.10358910054438955	No Hit
ATCCTTCGAGGGCTTTGTCTGACAACTGTCTTATCTTCTTTTGTAAGTGG	9329	0.10304784804634358	No Hit
CACACTGTCATTAGTTGCAGCATCTCCTGTAGCAATTGGGCTAAATCTGT	9310	0.10283797462873392	No Hit
TAATTCTGAACTGGAAAAGCCCCAGAAAGTCCGGAAAGACAAGGAAGGAA	9234	0.10199848095829528	No Hit
AGACTGCTGAAGTTCTTCTTTGTCCCACTGAGGTTGTACTCTTCATTCTC	9075	0.10024217183198286	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	3759725	3.0662794	4.678375	75-79
CCCAG	1844505	2.861482	5.1875157	2
GGAGG	1523845	2.472941	5.169821	4
CCCCA	1610380	2.4610415	6.391891	1
CCAGC	1564065	2.4264202	5.110786	5
CCTCC	1523580	2.3504424	5.337287	6
GGGGG	1190595	2.3219805	5.88038	4
TGGGG	1414035	2.316471	8.171983	3
CTGCC	1471910	2.3050811	5.989555	7
CCACC	1343335	2.0529337	6.0087376	1
GAGGG	1231375	1.998312	5.363626	8
CCCTG	1259590	1.9725779	5.6570177	1
TGGCT	1454190	1.9712402	6.4574814	3
TGCAG	1441055	1.9351078	5.9193277	3
GCCCA	1163995	1.8057694	5.72103	2
GGCTC	1128940	1.7947187	7.3398814	1
GCTCC	1026915	1.6081976	6.870792	2
TGCCC	987265	1.546104	8.792442	3
TAAAA	1934150	1.5190451	6.1648374	1
CATGG	1106660	1.4860685	37.218044	1
ATGGA	1309915	1.485822	10.571826	2
GGCCC	761320	1.4193842	6.2970996	1
TGGGT	964645	1.3274143	5.4927797	3
ATGTG	1140845	1.3063034	9.617377	2
ATGGG	939265	1.2803637	11.257433	2
ATGGC	946520	1.2710259	10.76388	2
ATGAA	1276320	1.2046522	8.3589	2
ATGCC	871040	1.1522378	8.089551	2
TGAGC	849555	1.1408173	5.1671515	1
ATGTT	1138535	1.0950552	6.451807	2
ATGCT	957025	1.0794936	7.8581176	2
CATGA	965575	1.0789194	24.33851	1
CATGT	941515	1.0619988	28.458042	1
ATGAG	910570	1.0328493	5.317975	2
ATGGT	865910	0.9914942	6.566939	2
CATGC	725395	0.9595742	28.069077	1
ATGAC	842400	0.94128567	6.3304787	2
ATGTA	906700	0.8638921	6.952797	2
ATGCA	726845	0.81216604	9.807872	2
TAAGT	785700	0.7486048	6.2755117	1
>>END_MODULE