FastQCFastQC Report
Wed 20 Mar 2013
8016-7-10.h.sapiens.aligned.bam

Summary

[OK] Basic Statistics

Measure Value
Filename 8016-7-10.h.sapiens.aligned.bam
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 9053076
Filtered Sequences 0
Sequence length 1-100
%GC 45

[WARN] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
CATGCACTATCCAGGCGCCTTCACCTACTCCCCGACGCCGGTCACCTCGGGCATCGGCATCGG 14726 0.16266294461683523 No Hit
CCGATGCCGATGCCCGAGGTGACCGGCGTCGGGGAGTAGGTGAAGGCGCCTGGATAGTGCATG 14247 0.1573719252992022 No Hit
GACAACTTGGCCACCGACAGCTGAAGGGCTTTTCACCTGTTGACACAATTGCCAGTCA 13331 0.1472538173765469 No Hit
TGACTGGCAATTGTGTCAACAGGTGAAAAGCCCTTCAGCTGTCGGTGGCCAAGTTGTC 13282 0.14671256487850096 No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG 12513 0.13821821444998364 No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA 12384 0.1367932844041075 No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG 11415 0.12608974010601479 No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC 11380 0.1257031311788391 No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT 11280 0.12459853424405141 No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT 11235 0.12410146562339697 No Hit
ATTCAGCTGCCAAGTAACTTAGCACACCCATTCTTATAAGATAATGGCAA 11130 0.12294163884186987 No Hit
TAATGAGCATGAAGTCACCACACGGAGGAAAATGTAAATGTGTAAACCTC 11077 0.1223562024664324 No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA 10806 0.11936274477315775 No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC 10735 0.11857848094945851 No Hit
GAGCTACCATCTGATCTGTCTGTCTTGACCACCCGGAGTCCCACTGTCCCCAGCCAGAATCCCAGTAGACTA 10621 0.11731924044380054 No Hit
TAGTCTACTGGGATTCTGGCTGGGGACAGTGGGACTCCGGGTGGTCAAGACAGACAGATCAGATGGTAGCTC 10467 0.11561816116422749 No Hit
CATGTAATTCAGGCAGTTATGGGAGCCCTAGAGGGCCTGAGAGTTGCTATTGGAC 9960 0.11001785470485391 No Hit
GTCCAATAGCAACTCTCAGGCCCTCTAGGGCTCCCATAACTGCCTGAATTACATG 9915 0.10952078608419945 No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA 9759 0.10779761486593065 No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC 9686 0.10699125910353564 No Hit
CAACACCCAACACTTATAAGCGGAAGAACACAGAAACAGCTCTAGACAAC 9487 0.10479311120330814 No Hit
CATGCTTTCCCTGGGTCTTCACGGATTGCTTTCCAAGCTGCCTTGTTGCG 9465 0.10455009987765485 No Hit
GCGACAGCTGGAAAGGTCCAGTTGCTGCTCTGCAGCAACCCCGCAACAAG 9388 0.10369956023786832 No Hit
CATGGAATATATATTCTCCTGGCTGGTGCTTATGATTGCAAAACTTGGAT 9378 0.10358910054438955 No Hit
ATCCTTCGAGGGCTTTGTCTGACAACTGTCTTATCTTCTTTTGTAAGTGG 9329 0.10304784804634358 No Hit
CACACTGTCATTAGTTGCAGCATCTCCTGTAGCAATTGGGCTAAATCTGT 9310 0.10283797462873392 No Hit
TAATTCTGAACTGGAAAAGCCCCAGAAAGTCCGGAAAGACAAGGAAGGAA 9234 0.10199848095829528 No Hit
AGACTGCTGAAGTTCTTCTTTGTCCCACTGAGGTTGTACTCTTCATTCTC 9075 0.10024217183198286 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 3759725 3.0662794 4.678375 75-79
CCCAG 1844505 2.861482 5.1875157 2
GGAGG 1523845 2.472941 5.169821 4
CCCCA 1610380 2.4610415 6.391891 1
CCAGC 1564065 2.4264202 5.110786 5
CCTCC 1523580 2.3504424 5.337287 6
GGGGG 1190595 2.3219805 5.88038 4
TGGGG 1414035 2.316471 8.171983 3
CTGCC 1471910 2.3050811 5.989555 7
CCACC 1343335 2.0529337 6.0087376 1
GAGGG 1231375 1.998312 5.363626 8
CCCTG 1259590 1.9725779 5.6570177 1
TGGCT 1454190 1.9712402 6.4574814 3
TGCAG 1441055 1.9351078 5.9193277 3
GCCCA 1163995 1.8057694 5.72103 2
GGCTC 1128940 1.7947187 7.3398814 1
GCTCC 1026915 1.6081976 6.870792 2
TGCCC 987265 1.546104 8.792442 3
TAAAA 1934150 1.5190451 6.1648374 1
CATGG 1106660 1.4860685 37.218044 1
ATGGA 1309915 1.485822 10.571826 2
GGCCC 761320 1.4193842 6.2970996 1
TGGGT 964645 1.3274143 5.4927797 3
ATGTG 1140845 1.3063034 9.617377 2
ATGGG 939265 1.2803637 11.257433 2
ATGGC 946520 1.2710259 10.76388 2
ATGAA 1276320 1.2046522 8.3589 2
ATGCC 871040 1.1522378 8.089551 2
TGAGC 849555 1.1408173 5.1671515 1
ATGTT 1138535 1.0950552 6.451807 2
ATGCT 957025 1.0794936 7.8581176 2
CATGA 965575 1.0789194 24.33851 1
CATGT 941515 1.0619988 28.458042 1
ATGAG 910570 1.0328493 5.317975 2
ATGGT 865910 0.9914942 6.566939 2
CATGC 725395 0.9595742 28.069077 1
ATGAC 842400 0.94128567 6.3304787 2
ATGTA 906700 0.8638921 6.952797 2
ATGCA 726845 0.81216604 9.807872 2
TAAGT 785700 0.7486048 6.2755117 1
Produced by FastQC (version 0.10.0)