##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	8016-7-11.h.sapiens.aligned.bam	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	7538824	
Filtered Sequences	0	
Sequence length	1-100	
%GC	46	
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.840591450337612	24.0	24.0	32.0	24.0	32.0
2	31.018768188484934	31.0	31.0	32.0	30.0	32.0
3	32.71679652654075	34.0	33.0	34.0	30.0	34.0
4	34.28241209736287	35.0	35.0	35.0	29.0	37.0
5	35.400677564561306	36.0	36.0	36.0	34.0	36.0
6	35.58107302721334	36.0	36.0	37.0	34.0	37.0
7	35.9362062591552	37.0	35.0	37.0	33.0	38.0
8	36.28013454221649	37.0	36.0	37.0	34.0	38.0
9	36.522289838804824	37.0	37.0	38.0	35.0	38.0
10-14	36.79459807797237	37.6	36.8	38.0	34.8	38.2
15-19	37.29620315938843	38.0	37.0	38.8	34.8	39.2
20-24	36.920873702540064	37.8	36.8	38.2	35.6	38.6
25-29	37.05005441581638	38.0	36.8	38.6	34.2	39.0
30-34	37.04833487202658	37.8	36.8	38.8	35.4	39.2
35-39	36.96155348556781	38.0	37.2	38.6	33.8	39.2
40-44	36.83151616825147	38.0	36.8	38.2	33.6	39.2
45-49	36.693158782623684	37.4	37.0	38.4	33.8	39.2
50-54	36.94027433868077	38.2	37.2	38.2	34.0	39.0
55-59	36.99766248983953	38.0	37.2	38.4	33.4	38.8
60-64	36.48263812662348	37.4	36.4	38.0	33.4	38.4
65-69	36.57906923488709	37.8	36.4	38.4	34.0	38.8
70-74	36.626712541510095	37.6	36.8	38.6	34.2	38.6
75-79	36.290464041472795	37.2	36.8	37.8	34.4	38.6
80-84	36.534150504847446	37.8	36.4	38.0	34.0	38.8
85-89	36.46394275522287	38.0	36.2	38.6	33.0	39.2
90-94	36.26239075738757	37.6	36.4	38.4	33.2	39.6
95-99	35.95276334209787	37.2	36.4	38.0	32.6	38.6
100	34.95042096415598	37.0	35.0	37.0	32.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
1	1.0
2	0.0
3	2.0
4	0.0
5	13.0
6	78.0
7	146.0
8	260.0
9	455.0
10	545.0
11	748.0
12	1025.0
13	1237.0
14	1637.0
15	2045.0
16	2500.0
17	3055.0
18	3952.0
19	4098.0
20	4050.0
21	4936.0
22	6051.0
23	7713.0
24	10242.0
25	14105.0
26	19254.0
27	26523.0
28	36646.0
29	48522.0
30	64390.0
31	86331.0
32	116549.0
33	160254.0
34	241984.0
35	469317.0
36	1585776.0
37	4612085.0
38	2299.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.306370292166626	15.797019349190029	21.073662221659582	39.82294813698377
2	19.885135833403975	46.803552744533754	17.223681726602745	16.087629695459523
3	21.80433174924803	31.602680895798578	29.343061142597527	17.249926212355867
4	34.63554662368758	23.15384258245949	20.14921967752419	22.06139111632874
5	22.49275900605876	26.07052300304762	24.733152709196244	26.70356528169738
6	22.396758643844134	27.66731846995446	24.921347846601737	25.014575039599666
7	23.092835743880446	26.136936585598797	25.925270054896405	24.844957615624352
8	22.78261838484451	25.755867591765373	27.121986614323113	24.339527409067003
9	22.93650694691133	28.003198409921225	25.8512622656341	23.209032377533344
10-14	23.493324966925417	26.04059320845907	25.67418879323692	24.791893031378592
15-19	22.54824549964137	26.250442335373698	26.705904824683955	24.495407340300975
20-24	22.87336223731439	25.25567467360965	26.640471803653316	25.230491285422644
25-29	22.911651782031335	26.212978392961432	27.411792936259772	23.46357688874746
30-34	22.607651823474946	27.27507164776064	26.68933251293234	23.42794401583207
35-39	23.005706410698373	26.452023631279943	27.56104578813245	22.981224169889238
40-44	22.574917594467543	27.194489157881907	26.969606869455966	23.26098637819458
45-49	22.82701647310385	27.386955457538846	27.248192763666303	22.537835305691
50-54	22.95625748020214	27.170510110616693	27.228319842313997	22.64491256686717
55-59	22.720908119179256	27.696171917782387	27.068707014066362	22.51421294897199
60-64	22.575338022367834	27.15967755702731	27.773227087615464	22.491757332989387
65-69	23.040389193347085	27.542980215981324	27.569427950438246	21.84720264023334
70-74	22.59647761902834	27.596534069982287	27.166454344559725	22.640533966429643
75-79	22.985201015693097	27.723844267616283	26.941052839975367	22.349901876715258
80-84	22.50241025822674	27.625951096117134	26.99759012798838	22.87404851766774
85-89	22.86903635180327	27.602829670362382	27.00757421884075	22.520559758993596
90-94	22.6673029419929	27.114097542551814	27.385339480995093	22.833260034460192
95-99	22.795517278915224	26.624489224213022	27.410080951095583	23.169912545776178
100	23.606923597094365	26.087422899290114	26.599511643864986	23.706141859750534
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	63.129318429150395
2	35.97276552886349
3	39.054257961603895
4	56.69693774001632
5	49.19632428775614
6	47.4113336834438
7	47.9377933595048
8	47.122145793911514
9	46.14553932444467
10-14	48.285217998304006
15-19	47.04365283994235
20-24	48.10385352273703
25-29	46.375228670778796
30-34	46.03559583930702
35-39	45.98693058058761
40-44	45.83590397266213
45-49	45.36485177879485
50-54	45.60117004706931
55-59	45.235121068151244
60-64	45.067095355357225
65-69	44.88759183358042
70-74	45.23701158545798
75-79	45.33510289240836
80-84	45.376458775894484
85-89	45.389596110796866
90-94	45.50056297645309
95-99	45.9654298246914
100	47.313065456844896
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	59.0
1	61.0
2	63.0
3	64.0
4	65.5
5	68.0
6	71.0
7	80.0
8	99.5
9	126.5
10	155.0
11	176.0
12	203.0
13	261.5
14	300.5
15	548.5
16	777.0
17	892.5
18	1234.5
19	2182.5
20	3288.5
21	3846.5
22	5671.5
23	10833.5
24	20435.5
25	30730.5
26	40220.0
27	48601.0
28	59678.0
29	79664.0
30	106826.5
31	122550.0
32	134255.5
33	150872.0
34	178193.0
35	194327.0
36	183246.5
37	185653.5
38	212648.0
39	244388.0
40	281287.0
41	280764.5
42	255780.0
43	291555.5
44	325223.0
45	314344.0
46	281705.5
47	242553.0
48	237381.0
49	254469.0
50	278088.0
51	301841.5
52	257689.5
53	189146.5
54	170506.5
55	157869.0
56	125691.0
57	101014.5
58	126961.0
59	136297.0
60	125836.0
61	132491.0
62	114653.5
63	92876.0
64	75120.0
65	71709.0
66	91068.5
67	85625.5
68	54853.0
69	39807.5
70	34024.0
71	28628.5
72	24494.5
73	15191.5
74	10579.5
75	12275.0
76	11388.5
77	10068.0
78	7361.5
79	6751.0
80	6274.0
81	5409.5
82	3742.5
83	2251.5
84	2124.0
85	1270.0
86	2234.0
87	1608.5
88	388.0
89	269.5
90	178.5
91	129.5
92	76.5
93	54.5
94	44.5
95	36.5
96	29.5
97	25.5
98	29.0
99	19.0
100	9.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.038507332178069155
2	0.010877110275460166
3	0.0032100740081236097
4	0.0028784547924083606
5	0.0011938294932923362
6	0.002546836252356984
7	5.305920120168479E-5
8	0.0012468912282395926
9	3.979440090126359E-5
10-14	3.528438377641013E-4
15-19	0.012044452093231593
20-24	0.011946817955884325
25-29	0.00797055183574359
30-34	0.009711398722409777
35-39	0.0057845434293039505
40-44	0.01027177154518676
45-49	0.009966927367980471
50-54	0.00954057912590915
55-59	0.009530028234378938
60-64	0.03849567556429652
65-69	0.02975434922208162
70-74	0.018473680922058342
75-79	0.0385737733588186
80-84	0.04451905127240798
85-89	0.012384622004059433
90-94	0.0025969180221305744
95-99	0.006464466551039227
100	0.011280976214659885
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
0-1	57.0
2-3	0.0
4-5	2.0
6-7	16.0
8-9	0.0
10-11	4.0
12-13	0.0
14-15	0.0
16-17	4.0
18-19	34.0
20-21	381.0
22-23	116.0
24-25	430.0
26-27	80.0
28-29	63.0
30-31	126.0
32-33	71.0
34-35	76.0
36-37	62.0
38-39	54.0
40-41	99.0
42-43	82.0
44-45	86.0
46-47	106.0
48-49	132.0
50-51	14837.0
52-53	574.0
54-55	17273.0
56-57	27965.0
58-59	57882.0
60-61	1239.0
62-63	27388.0
64-65	16001.0
66-67	12823.0
68-69	13821.0
70-71	26902.0
72-73	31251.0
74-75	10761.0
76-77	9360.0
78-79	4156.0
80-81	10590.0
82-83	18119.0
84-85	8734.0
86-87	4647.0
88-89	8766.0
90-91	21032.0
92-93	406.0
94-95	8040.0
96-97	163507.0
98-99	0.0
100-101	7020669.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	98.43232802153972
#Duplication Level	Relative count
1	100.0
2	12.874839233737223
3	4.0056183578149325
4	1.681276653354092
5	0.8326000135382116
6	0.5423746023150342
7	0.35030122520815
8	0.25891829689298046
9	0.175150612604075
10++	7.477323495566235
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CATGCACTATCCAGGCGCCTTCACCTACTCCCCGACGCCGGTCACCTCGGGCATCGGCATCGG	11030	0.14630929174099302	No Hit
CCGATGCCGATGCCCGAGGTGACCGGCGTCGGGGAGTAGGTGAAGGCGCCTGGATAGTGCATG	10649	0.14125545310515272	No Hit
TGACTGGCAATTGTGTCAACAGGTGAAAAGCCCTTCAGCTGTCGGTGGCCAAGTTGTC	10202	0.1353261463591669	No Hit
GACAACTTGGCCACCGACAGCTGAAGGGCTTTTCACCTGTTGACACAATTGCCAGTCA	10193	0.13520676434414705	No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG	9960	0.1321160966219665	No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA	9830	0.13039168973834647	No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC	9129	0.12109315723513375	No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG	9123	0.12101356922512051	No Hit
GAGCTACCATCTGATCTGTCTGTCTTGACCACCCGGAGTCCCACTGTCCCCAGCCAGAATCCCAGTAGACTA	9104	0.12076154052674529	No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT	9026	0.11972689639657326	No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT	9000	0.11938201501984924	No Hit
TAGTCTACTGGGATTCTGGCTGGGGACAGTGGGACTCCGGGTGGTCAAGACAGACAGATCAGATGGTAGCTC	8963	0.11889122229143431	No Hit
ATTCAGCTGCCAAGTAACTTAGCACACCCATTCTTATAAGATAATGGCAA	8325	0.11042836389336057	No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA	8295	0.11003042384329438	No Hit
TAATGAGCATGAAGTCACCACACGGAGGAAAATGTAAATGTGTAAACCTC	8276	0.10977839514491915	No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC	8271	0.10971207180324145	No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT	7753	0.10284097360543235	No Hit
GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG	7731	0.10254915090205051	No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA	7626	0.10115636072681894	No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG	7623	0.10111656672181231	No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA	7612	0.1009706553701214	No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC	7582	0.10057271532005521	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	3114865	3.1677408	4.9201937	75-79
AAAAA	3111180	3.010305	4.102711	2
CCCAG	1605765	2.8989747	5.1954417	2
CCCCA	1404890	2.4989219	6.010461	1
CCAGC	1333460	2.4073677	5.194708	5
CCTCC	1304865	2.3442342	5.3431873	6
TGGGG	1224630	2.3347936	8.095843	3
CTGCC	1268920	2.3137777	5.8767433	7
GGGGG	1019590	2.2745867	5.6082087	4
CCACC	1191175	2.1187804	5.9491215	1
GGAAG	1321220	2.1102571	5.020512	4
TGGCT	1240485	1.9913601	6.201239	3
CCCTG	1083580	1.9758247	5.2010345	1
TGCAG	1208810	1.9212828	5.86736	3
GCCCA	1010890	1.8250145	5.678838	2
TGGCA	1131560	1.7985016	5.071086	3
GGCTC	969735	1.7947028	7.3981256	1
GCTCC	898815	1.63892	7.159906	2
TGCCC	863560	1.5746351	9.545005	3
TAAAA	1571640	1.5359017	5.739783	1
TGAGG	915195	1.4763825	5.047185	1
CATGG	927510	1.4741845	36.506924	1
ATGGA	1066210	1.4553577	10.250294	2
GGCCC	660145	1.4085104	5.717981	1
TGGCC	733405	1.3573235	5.213587	3
TGGGT	811820	1.3227265	5.6585717	3
ATGGG	811225	1.3086593	11.551987	2
ATGTG	944510	1.3021425	9.398456	2
ATGGC	812385	1.2912047	10.995841	2
ATGAA	1024480	1.183238	7.872505	2
ATGCC	738465	1.1564081	9.105476	2
TGAGC	710210	1.1288078	5.112411	1
ATGTT	937790	1.1049032	6.2615695	2
ATGCT	791615	1.0752611	8.014821	2
CATGA	797065	1.0719355	23.255438	1
CATGT	782230	1.0625134	27.746927	1
ATGAG	755585	1.0313599	5.4783826	2
ATGGT	721765	0.99505657	6.295585	2
CATGC	625770	0.979932	28.781073	1
ATGAC	692920	0.9318757	6.0510993	2
ATGTA	749035	0.87376726	7.1057076	2
ATGCA	604460	0.81291	9.89845	2
TAAGT	640935	0.747666	6.0705323	1
>>END_MODULE