FastQCFastQC Report
Wed 20 Mar 2013
8016-7-11.h.sapiens.aligned.bam

Summary

[OK] Basic Statistics

Measure Value
Filename 8016-7-11.h.sapiens.aligned.bam
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 7538824
Filtered Sequences 0
Sequence length 1-100
%GC 46

[WARN] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
CATGCACTATCCAGGCGCCTTCACCTACTCCCCGACGCCGGTCACCTCGGGCATCGGCATCGG 11030 0.14630929174099302 No Hit
CCGATGCCGATGCCCGAGGTGACCGGCGTCGGGGAGTAGGTGAAGGCGCCTGGATAGTGCATG 10649 0.14125545310515272 No Hit
TGACTGGCAATTGTGTCAACAGGTGAAAAGCCCTTCAGCTGTCGGTGGCCAAGTTGTC 10202 0.1353261463591669 No Hit
GACAACTTGGCCACCGACAGCTGAAGGGCTTTTCACCTGTTGACACAATTGCCAGTCA 10193 0.13520676434414705 No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG 9960 0.1321160966219665 No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA 9830 0.13039168973834647 No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC 9129 0.12109315723513375 No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG 9123 0.12101356922512051 No Hit
GAGCTACCATCTGATCTGTCTGTCTTGACCACCCGGAGTCCCACTGTCCCCAGCCAGAATCCCAGTAGACTA 9104 0.12076154052674529 No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT 9026 0.11972689639657326 No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT 9000 0.11938201501984924 No Hit
TAGTCTACTGGGATTCTGGCTGGGGACAGTGGGACTCCGGGTGGTCAAGACAGACAGATCAGATGGTAGCTC 8963 0.11889122229143431 No Hit
ATTCAGCTGCCAAGTAACTTAGCACACCCATTCTTATAAGATAATGGCAA 8325 0.11042836389336057 No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA 8295 0.11003042384329438 No Hit
TAATGAGCATGAAGTCACCACACGGAGGAAAATGTAAATGTGTAAACCTC 8276 0.10977839514491915 No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC 8271 0.10971207180324145 No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT 7753 0.10284097360543235 No Hit
GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG 7731 0.10254915090205051 No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA 7626 0.10115636072681894 No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG 7623 0.10111656672181231 No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA 7612 0.1009706553701214 No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC 7582 0.10057271532005521 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 3114865 3.1677408 4.9201937 75-79
AAAAA 3111180 3.010305 4.102711 2
CCCAG 1605765 2.8989747 5.1954417 2
CCCCA 1404890 2.4989219 6.010461 1
CCAGC 1333460 2.4073677 5.194708 5
CCTCC 1304865 2.3442342 5.3431873 6
TGGGG 1224630 2.3347936 8.095843 3
CTGCC 1268920 2.3137777 5.8767433 7
GGGGG 1019590 2.2745867 5.6082087 4
CCACC 1191175 2.1187804 5.9491215 1
GGAAG 1321220 2.1102571 5.020512 4
TGGCT 1240485 1.9913601 6.201239 3
CCCTG 1083580 1.9758247 5.2010345 1
TGCAG 1208810 1.9212828 5.86736 3
GCCCA 1010890 1.8250145 5.678838 2
TGGCA 1131560 1.7985016 5.071086 3
GGCTC 969735 1.7947028 7.3981256 1
GCTCC 898815 1.63892 7.159906 2
TGCCC 863560 1.5746351 9.545005 3
TAAAA 1571640 1.5359017 5.739783 1
TGAGG 915195 1.4763825 5.047185 1
CATGG 927510 1.4741845 36.506924 1
ATGGA 1066210 1.4553577 10.250294 2
GGCCC 660145 1.4085104 5.717981 1
TGGCC 733405 1.3573235 5.213587 3
TGGGT 811820 1.3227265 5.6585717 3
ATGGG 811225 1.3086593 11.551987 2
ATGTG 944510 1.3021425 9.398456 2
ATGGC 812385 1.2912047 10.995841 2
ATGAA 1024480 1.183238 7.872505 2
ATGCC 738465 1.1564081 9.105476 2
TGAGC 710210 1.1288078 5.112411 1
ATGTT 937790 1.1049032 6.2615695 2
ATGCT 791615 1.0752611 8.014821 2
CATGA 797065 1.0719355 23.255438 1
CATGT 782230 1.0625134 27.746927 1
ATGAG 755585 1.0313599 5.4783826 2
ATGGT 721765 0.99505657 6.295585 2
CATGC 625770 0.979932 28.781073 1
ATGAC 692920 0.9318757 6.0510993 2
ATGTA 749035 0.87376726 7.1057076 2
ATGCA 604460 0.81291 9.89845 2
TAAGT 640935 0.747666 6.0705323 1
Produced by FastQC (version 0.10.0)