##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	8016-7-12.h.sapiens.aligned.bam	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	8009086	
Filtered Sequences	0	
Sequence length	1-100	
%GC	46	
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.836090535174677	24.0	24.0	32.0	24.0	32.0
2	31.011418922057352	31.0	31.0	32.0	30.0	32.0
3	32.711203359499514	34.0	33.0	34.0	30.0	34.0
4	34.27632325493469	35.0	35.0	35.0	29.0	37.0
5	35.39979515018116	36.0	36.0	36.0	34.0	36.0
6	35.58014225380532	36.0	36.0	37.0	34.0	37.0
7	35.934830496559634	37.0	35.0	37.0	33.0	38.0
8	36.276163584161615	37.0	36.0	37.0	34.0	38.0
9	36.511947066855925	37.0	37.0	38.0	35.0	38.0
10-14	36.78338444205568	37.6	36.8	38.0	34.8	38.2
15-19	37.28604427059984	38.0	37.0	38.8	34.8	39.2
20-24	36.907295150917506	37.8	36.6	38.2	35.6	38.6
25-29	37.032388865149514	38.0	36.8	38.6	34.2	39.0
30-34	37.02404746648191	37.8	36.8	38.8	35.4	39.2
35-39	36.929246351804665	38.0	37.2	38.6	33.6	39.2
40-44	36.803163102361594	38.0	36.8	38.2	33.6	39.2
45-49	36.66751390976502	37.2	37.0	38.4	33.8	39.2
50-54	36.91301596196164	38.2	37.2	38.2	34.0	39.0
55-59	36.96908764646862	38.0	37.2	38.4	33.0	38.8
60-64	36.462091622176914	37.4	36.4	38.0	33.4	38.4
65-69	36.552681173979295	37.8	36.4	38.4	34.0	38.8
70-74	36.60158473114608	37.6	36.8	38.6	34.2	38.6
75-79	36.26144972772128	37.2	36.8	37.8	34.2	38.6
80-84	36.50617840492693	37.8	36.4	38.0	34.0	38.8
85-89	36.42503420277832	38.0	36.2	38.6	33.0	39.2
90-94	36.22200662818943	37.6	36.4	38.4	33.2	39.6
95-99	35.9096866266715	37.2	36.2	38.0	32.4	38.6
100	34.89377155581344	37.0	35.0	37.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
1	1.0
2	0.0
3	19.0
4	1.0
5	17.0
6	96.0
7	204.0
8	311.0
9	688.0
10	656.0
11	904.0
12	1215.0
13	1507.0
14	1893.0
15	2299.0
16	2801.0
17	3513.0
18	4612.0
19	4797.0
20	4433.0
21	5355.0
22	6594.0
23	8348.0
24	11220.0
25	15267.0
26	20927.0
27	29234.0
28	39615.0
29	52481.0
30	69348.0
31	93832.0
32	125430.0
33	172672.0
34	264134.0
35	513312.0
36	1707513.0
37	4841317.0
38	2520.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.251800814946286	15.750665058974993	20.77674591330831	40.22078821277041
2	19.6922098276919	46.987702054577696	16.692205956525804	16.627882161204596
3	22.104073773978847	31.063502106306384	29.165547552877424	17.66687656683735
4	35.11561713145147	22.75786675357384	20.006705337860996	22.119810777113695
5	22.986717318256062	25.776121689677687	24.24976890753417	26.98739208453208
6	22.764535645622672	27.38768252163838	24.344880004185516	25.50290182855343
7	23.32050234156859	25.874876104013445	25.668301631693595	25.136319922724365
8	23.22059764424539	25.444881805209473	26.836128563087584	24.498391987457552
9	23.432824639081804	27.452470675786707	25.334122471200217	23.780582213931282
10-14	23.75905895590303	25.757109976490078	25.336832259659875	25.146998807947014
15-19	22.908567598716395	25.72463745492967	26.274516366181167	25.092278580172767
20-24	23.23434621806507	24.937469299789793	26.114713782556898	25.713470699588242
25-29	23.041614650414562	25.898885052339004	27.19676454790089	23.86273574934554
30-34	23.07063099938721	26.736099773662968	26.48943465306684	23.703834573882983
35-39	23.22164132663373	26.16177632635767	27.265784672467408	23.350797674541194
40-44	22.970171361430914	26.891418426969032	26.711062168643345	23.42734804295671
45-49	23.22090793246241	26.995830532292597	26.909337026201452	22.87392450904354
50-54	23.288577033952595	26.765119514734142	26.925110606181935	23.021192845131324
55-59	23.031836886804932	27.346985996911915	26.59998332941736	23.021193786865794
60-64	23.07730022476093	26.687540171773755	27.471101893403098	22.764057710062215
65-69	23.475266734729672	27.211814124268756	27.127747585707134	22.185171555294435
70-74	22.890509751464425	27.339116838495325	26.7294842067549	23.04088920328535
75-79	23.35771804690851	27.430356974135083	26.636911577670457	22.575013401285947
80-84	22.719712568175837	27.2876273381362	26.70393758583989	23.288722507848078
85-89	23.212584747112682	27.254439022555882	26.85342673164576	22.679549498685674
90-94	23.124064221040346	26.67448089951488	26.996009351097744	23.205445528347028
95-99	23.152657275062	26.330052940558417	27.130705598098537	23.386584186281045
100	24.18204411166107	25.726165636881014	26.08060390590704	24.011186345550875
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	63.472589027716694
2	36.32009198889649
3	39.77095034081619
4	57.235427908565164
5	49.974109402788145
6	48.267437474176106
7	48.45682226429296
8	47.71898963170295
9	47.21340685301308
10-14	48.90605776385004
15-19	48.00084617888916
20-24	48.947816917653306
25-29	46.904350399760105
30-34	46.77446557327019
35-39	46.57243900117492
40-44	46.39751940438762
45-49	46.09483244150595
50-54	46.30976987908392
55-59	46.053030673670726
60-64	45.841357934823144
65-69	45.66043829002411
70-74	45.931398954749774
75-79	45.93273144819446
80-84	46.008435076023915
85-89	45.89213424579835
90-94	46.32950974938738
95-99	46.539241461343046
100	48.193230457211946
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	273.0
1	272.0
2	272.0
3	276.5
4	281.0
5	283.0
6	287.5
7	293.5
8	318.0
9	343.0
10	360.5
11	398.0
12	429.0
13	482.0
14	525.5
15	766.0
16	963.5
17	1026.0
18	1330.5
19	2037.5
20	3163.5
21	4038.0
22	6519.0
23	11343.0
24	21919.0
25	32840.0
26	42749.0
27	54286.5
28	61984.0
29	76229.0
30	100512.5
31	118167.5
32	131227.0
33	148655.0
34	173946.0
35	187021.5
36	180912.5
37	182616.5
38	208451.0
39	248545.5
40	286171.0
41	289752.0
42	266683.5
43	295129.0
44	335840.0
45	332283.0
46	298860.5
47	254313.5
48	255805.0
49	281087.0
50	304281.0
51	329260.5
52	281805.5
53	206864.0
54	188429.0
55	175582.0
56	141875.0
57	114811.0
58	141756.0
59	154168.5
60	141400.5
61	148927.5
62	131809.0
63	107274.5
64	87825.5
65	84955.5
66	109023.5
67	105709.5
68	67858.5
69	48807.5
70	41910.5
71	36251.0
72	32820.5
73	20654.5
74	12516.0
75	14935.5
76	14778.5
77	13359.0
78	9861.5
79	8243.0
80	7686.0
81	6483.0
82	4212.0
83	3234.0
84	3052.5
85	1812.5
86	3413.0
87	2447.5
88	564.0
89	407.5
90	291.0
91	233.5
92	150.5
93	105.0
94	83.0
95	73.5
96	65.5
97	58.0
98	53.0
99	48.5
100	44.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.039892192442433506
2	0.011100273049236267
3	0.0030341578751005766
4	0.003333827788690757
5	0.0011861960739656932
6	0.002659579781400023
7	1.4983619158355128E-4
8	0.0012736076284601858
9	4.994540966723371E-5
10-14	3.845800770139085E-4
15-19	0.011764698243606219
20-24	0.011581216420841174
25-29	0.008782474736522012
30-34	0.009981925869712137
35-39	0.006412562807580374
40-44	0.010267469837621088
45-49	0.010115453827749936
50-54	0.01015371747413742
55-59	0.01032241413837561
60-64	0.03869189993353478
65-69	0.02936488389728221
70-74	0.01788653386009777
75-79	0.039252294120459254
80-84	0.045538458785236706
85-89	0.012444479630402612
90-94	0.0028469027622944885
95-99	0.006914884241948857
100	0.012600363437958843
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
0-1	274.0
2-3	0.0
4-5	28.0
6-7	38.0
8-9	2.0
10-11	12.0
12-13	0.0
14-15	10.0
16-17	8.0
18-19	119.0
20-21	1087.0
22-23	177.0
24-25	408.0
26-27	138.0
28-29	125.0
30-31	240.0
32-33	101.0
34-35	125.0
36-37	74.0
38-39	96.0
40-41	178.0
42-43	130.0
44-45	112.0
46-47	73.0
48-49	165.0
50-51	16324.0
52-53	588.0
54-55	16563.0
56-57	31427.0
58-59	69950.0
60-61	1079.0
62-63	29867.0
64-65	16824.0
66-67	12444.0
68-69	18134.0
70-71	26584.0
72-73	38253.0
74-75	13216.0
76-77	10297.0
78-79	4686.0
80-81	10121.0
82-83	14838.0
84-85	10441.0
86-87	5873.0
88-89	8817.0
90-91	19648.0
92-93	2171.0
94-95	8688.0
96-97	166367.0
98-99	0.0
100-101	7452166.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	98.4626327961182
#Duplication Level	Relative count
1	100.0
2	12.855622964533133
3	4.552136377295357
4	2.027954421785282
5	1.0817922666471749
6	0.630233332521177
7	0.4141997417342787
8	0.3069951026971713
9	0.20303908908543072
10++	7.6781261928546485
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TGACTGGCAATTGTGTCAACAGGTGAAAAGCCCTTCAGCTGTCGGTGGCCAAGTTGTC	12760	0.15931905338511784	No Hit
GACAACTTGGCCACCGACAGCTGAAGGGCTTTTCACCTGTTGACACAATTGCCAGTCA	12758	0.1592940817466562	No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG	12707	0.15865730496588498	No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA	12522	0.15634742840818538	No Hit
CATGCACTATCCAGGCGCCTTCACCTACTCCCCGACGCCGGTCACCTCGGGCATCGGCATCGG	12419	0.15506138902741212	No Hit
CCGATGCCGATGCCCGAGGTGACCGGCGTCGGGGAGTAGGTGAAGGCGCCTGGATAGTGCATG	11972	0.14948022783124068	No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG	11924	0.14888090850816185	No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC	11920	0.14883096523123862	No Hit
GAGCTACCATCTGATCTGTCTGTCTTGACCACCCGGAGTCCCACTGTCCCCAGCCAGAATCCCAGTAGACTA	11538	0.14406138228506973	No Hit
TAGTCTACTGGGATTCTGGCTGGGGACAGTGGGACTCCGGGTGGTCAAGACAGACAGATCAGATGGTAGCTC	11333	0.14150178934275395	No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT	10808	0.13494673424657944	No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT	10787	0.13468453204273245	No Hit
ATTCAGCTGCCAAGTAACTTAGCACACCCATTCTTATAAGATAATGGCAA	9949	0.12422141552731485	No Hit
TAATGAGCATGAAGTCACCACACGGAGGAAAATGTAAATGTGTAAACCTC	9879	0.12334740818115825	No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT	9762	0.12188656733115365	No Hit
GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG	9681	0.12087521597345814	No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA	9617	0.1200761235426864	No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC	9614	0.12003866608499397	No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA	9253	0.11553128534267207	No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG	9240	0.11536896969267155	No Hit
CATGTCATCTCAAAGGTCCCCAGGATTCGAACACCCAGTTATTCTCCAAC	8985	0.11218508578881535	No Hit
AGCCACCTCACCCACCCGGAATCTACATTTTCAATCAGTAAAAGTCACCA	8970	0.11199779850035321	No Hit
ACCTATGTTACACCATCTTCAGTGCCAGTCTTGGGCAAAATTGTGCAAGA	8780	0.1096254928464996	No Hit
GCCTCAGCCTTCCTTCGCAGAAAGTTGAGCAGGTCGCCATAGCAACAGTA	8751	0.10926340408880615	No Hit
CATGGAGGGGAGCACTCAGCACCTCCCTCACCCCACACCCTTGGCTGCTC	8750	0.10925091826957532	No Hit
TAAAACTGCATCAAGTCATGGGGCATGTGGAAGGTAGGGAGGCAAGATGA	8729	0.10898871606572835	No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA	8583	0.1071657864580303	No Hit
GAAGGGCAGGAGTTCCTTTGGTGACTTCACAGTGAAGTCTTGCCCTCTCT	8554	0.10680369770033685	No Hit
TAGCTGCTCCATTTTCATTTCACCACTTTGAAATCTTGGAACCACTGATG	8543	0.10666635368879794	No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC	8526	0.1064540947618742	No Hit
GACCCCCTCCACTAACTCCCGAGGACGTTGGCTTTGCATCTGGTTTTTCT	8468	0.1057299172464873	No Hit
AGAAGAGGGCTCCTTTTGTGCATATCAAACCTTGTTCCAGAATGGAGTGG	8464	0.10567997396956406	No Hit
GATCCAAAACTTCTCTCCGACACTGAATACCTTCTGCTTCTCCTATCATT	8421	0.10514308374263928	No Hit
CATGTGCCTTCACTGTGTCCCAGGAAATCTGGGTTGGTTCCAGTGGGAAA	8103	0.10117259322724216	No Hit
CGGGGCTTGCTCTCCCCATCCCTATTTCCCCATCTCCTACTCTTTTGCTA	8048	0.1004858731695477	No Hit
CATGGCTCTGCTGTGCCTTCCATCCTGGGCTCCCTTCTCTCCTGTGACCT	8033	0.10029858588108556	No Hit
ACAGTGAAAACAGTGACAATGTTCCAGCAAAGCCACCAGACAAAGTTCTT	8024	0.10018621350800828	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	3158280	3.2236416	5.150065	75-79
AAAAA	3117720	3.03405	4.5530796	2
CCCAG	1780695	2.8912914	5.4751024	2
CCCCA	1560530	2.4961474	6.0827565	1
GGAGG	1436470	2.4395602	5.0465226	4
CCAGC	1500995	2.437146	5.156392	5
CCTCC	1462040	2.3610191	6.0015244	6
TGGGG	1363635	2.3380578	8.131808	3
GGGGG	1166075	2.2761393	5.8896227	4
CTGCC	1386345	2.272562	5.4399557	7
CTCCA	1479750	2.1104517	5.239836	6
GGAAG	1403505	2.0738227	5.1011176	4
CCACC	1286785	2.0582783	5.989129	1
TGGCT	1336890	1.983505	6.858871	3
TGCAG	1331595	1.9568956	5.2974443	3
CCCTG	1190530	1.9515729	5.5465803	1
GCCCA	1116530	1.8128952	5.9424706	2
GGCTC	1083185	1.8024006	7.651298	1
GCTCC	999375	1.6382226	7.548778	2
TAAAA	1616420	1.5881151	6.6176558	1
TGCCC	963925	1.5801113	9.408797	3
GATGG	1049220	1.5651871	5.1207843	1
ATGGA	1135075	1.4732178	10.434892	2
CATGG	998580	1.4675009	37.069817	1
GGCCC	762050	1.4221426	5.9762454	1
TGGGT	883430	1.3304973	5.5809784	3
TGCTC	901195	1.3172014	5.1069946	1
ATGTG	990815	1.298306	10.073626	2
ATGGG	869940	1.2977439	11.647854	2
ATGGC	861150	1.2655355	11.416458	2
TGAGA	950595	1.2337806	5.075343	1
ATGAA	1070645	1.2090118	8.760728	2
ATGCC	805810	1.1666052	8.971169	2
TGAGC	783110	1.1508489	5.1494145	1
ATGTT	958625	1.1033618	6.4957204	2
CATGA	851810	1.0891329	24.593292	1
ATGAG	817615	1.0611852	5.6682067	2
CATGT	815565	1.052783	28.134064	1
ATGCT	815445	1.052628	7.7754946	2
ATGGT	748930	0.98135406	6.7662463	2
CATGC	652745	0.9450066	27.223873	1
ATGAC	729580	0.9328484	6.6922927	2
ATGTA	745975	0.85045516	6.748772	2
TAATG	717375	0.81784946	5.490291	1
ATGCA	616310	0.7880202	9.409755	2
ATGTC	585360	0.7556197	5.1873174	2
TAAGT	652225	0.7435748	6.2092714	1
>>END_MODULE