FastQCFastQC Report
Wed 20 Mar 2013
8016-7-12.h.sapiens.aligned.bam

Summary

[OK] Basic Statistics

Measure Value
Filename 8016-7-12.h.sapiens.aligned.bam
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 8009086
Filtered Sequences 0
Sequence length 1-100
%GC 46

[WARN] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
TGACTGGCAATTGTGTCAACAGGTGAAAAGCCCTTCAGCTGTCGGTGGCCAAGTTGTC 12760 0.15931905338511784 No Hit
GACAACTTGGCCACCGACAGCTGAAGGGCTTTTCACCTGTTGACACAATTGCCAGTCA 12758 0.1592940817466562 No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG 12707 0.15865730496588498 No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA 12522 0.15634742840818538 No Hit
CATGCACTATCCAGGCGCCTTCACCTACTCCCCGACGCCGGTCACCTCGGGCATCGGCATCGG 12419 0.15506138902741212 No Hit
CCGATGCCGATGCCCGAGGTGACCGGCGTCGGGGAGTAGGTGAAGGCGCCTGGATAGTGCATG 11972 0.14948022783124068 No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG 11924 0.14888090850816185 No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC 11920 0.14883096523123862 No Hit
GAGCTACCATCTGATCTGTCTGTCTTGACCACCCGGAGTCCCACTGTCCCCAGCCAGAATCCCAGTAGACTA 11538 0.14406138228506973 No Hit
TAGTCTACTGGGATTCTGGCTGGGGACAGTGGGACTCCGGGTGGTCAAGACAGACAGATCAGATGGTAGCTC 11333 0.14150178934275395 No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT 10808 0.13494673424657944 No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT 10787 0.13468453204273245 No Hit
ATTCAGCTGCCAAGTAACTTAGCACACCCATTCTTATAAGATAATGGCAA 9949 0.12422141552731485 No Hit
TAATGAGCATGAAGTCACCACACGGAGGAAAATGTAAATGTGTAAACCTC 9879 0.12334740818115825 No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT 9762 0.12188656733115365 No Hit
GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG 9681 0.12087521597345814 No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA 9617 0.1200761235426864 No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC 9614 0.12003866608499397 No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA 9253 0.11553128534267207 No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG 9240 0.11536896969267155 No Hit
CATGTCATCTCAAAGGTCCCCAGGATTCGAACACCCAGTTATTCTCCAAC 8985 0.11218508578881535 No Hit
AGCCACCTCACCCACCCGGAATCTACATTTTCAATCAGTAAAAGTCACCA 8970 0.11199779850035321 No Hit
ACCTATGTTACACCATCTTCAGTGCCAGTCTTGGGCAAAATTGTGCAAGA 8780 0.1096254928464996 No Hit
GCCTCAGCCTTCCTTCGCAGAAAGTTGAGCAGGTCGCCATAGCAACAGTA 8751 0.10926340408880615 No Hit
CATGGAGGGGAGCACTCAGCACCTCCCTCACCCCACACCCTTGGCTGCTC 8750 0.10925091826957532 No Hit
TAAAACTGCATCAAGTCATGGGGCATGTGGAAGGTAGGGAGGCAAGATGA 8729 0.10898871606572835 No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA 8583 0.1071657864580303 No Hit
GAAGGGCAGGAGTTCCTTTGGTGACTTCACAGTGAAGTCTTGCCCTCTCT 8554 0.10680369770033685 No Hit
TAGCTGCTCCATTTTCATTTCACCACTTTGAAATCTTGGAACCACTGATG 8543 0.10666635368879794 No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC 8526 0.1064540947618742 No Hit
GACCCCCTCCACTAACTCCCGAGGACGTTGGCTTTGCATCTGGTTTTTCT 8468 0.1057299172464873 No Hit
AGAAGAGGGCTCCTTTTGTGCATATCAAACCTTGTTCCAGAATGGAGTGG 8464 0.10567997396956406 No Hit
GATCCAAAACTTCTCTCCGACACTGAATACCTTCTGCTTCTCCTATCATT 8421 0.10514308374263928 No Hit
CATGTGCCTTCACTGTGTCCCAGGAAATCTGGGTTGGTTCCAGTGGGAAA 8103 0.10117259322724216 No Hit
CGGGGCTTGCTCTCCCCATCCCTATTTCCCCATCTCCTACTCTTTTGCTA 8048 0.1004858731695477 No Hit
CATGGCTCTGCTGTGCCTTCCATCCTGGGCTCCCTTCTCTCCTGTGACCT 8033 0.10029858588108556 No Hit
ACAGTGAAAACAGTGACAATGTTCCAGCAAAGCCACCAGACAAAGTTCTT 8024 0.10018621350800828 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 3158280 3.2236416 5.150065 75-79
AAAAA 3117720 3.03405 4.5530796 2
CCCAG 1780695 2.8912914 5.4751024 2
CCCCA 1560530 2.4961474 6.0827565 1
GGAGG 1436470 2.4395602 5.0465226 4
CCAGC 1500995 2.437146 5.156392 5
CCTCC 1462040 2.3610191 6.0015244 6
TGGGG 1363635 2.3380578 8.131808 3
GGGGG 1166075 2.2761393 5.8896227 4
CTGCC 1386345 2.272562 5.4399557 7
CTCCA 1479750 2.1104517 5.239836 6
GGAAG 1403505 2.0738227 5.1011176 4
CCACC 1286785 2.0582783 5.989129 1
TGGCT 1336890 1.983505 6.858871 3
TGCAG 1331595 1.9568956 5.2974443 3
CCCTG 1190530 1.9515729 5.5465803 1
GCCCA 1116530 1.8128952 5.9424706 2
GGCTC 1083185 1.8024006 7.651298 1
GCTCC 999375 1.6382226 7.548778 2
TAAAA 1616420 1.5881151 6.6176558 1
TGCCC 963925 1.5801113 9.408797 3
GATGG 1049220 1.5651871 5.1207843 1
ATGGA 1135075 1.4732178 10.434892 2
CATGG 998580 1.4675009 37.069817 1
GGCCC 762050 1.4221426 5.9762454 1
TGGGT 883430 1.3304973 5.5809784 3
TGCTC 901195 1.3172014 5.1069946 1
ATGTG 990815 1.298306 10.073626 2
ATGGG 869940 1.2977439 11.647854 2
ATGGC 861150 1.2655355 11.416458 2
TGAGA 950595 1.2337806 5.075343 1
ATGAA 1070645 1.2090118 8.760728 2
ATGCC 805810 1.1666052 8.971169 2
TGAGC 783110 1.1508489 5.1494145 1
ATGTT 958625 1.1033618 6.4957204 2
CATGA 851810 1.0891329 24.593292 1
ATGAG 817615 1.0611852 5.6682067 2
CATGT 815565 1.052783 28.134064 1
ATGCT 815445 1.052628 7.7754946 2
ATGGT 748930 0.98135406 6.7662463 2
CATGC 652745 0.9450066 27.223873 1
ATGAC 729580 0.9328484 6.6922927 2
ATGTA 745975 0.85045516 6.748772 2
TAATG 717375 0.81784946 5.490291 1
ATGCA 616310 0.7880202 9.409755 2
ATGTC 585360 0.7556197 5.1873174 2
TAAGT 652225 0.7435748 6.2092714 1
Produced by FastQC (version 0.10.0)