##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	8016-7-14.h.sapiens.aligned.bam	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	7507752	
Filtered Sequences	0	
Sequence length	1-100	
%GC	46	
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.800868888583427	24.0	24.0	32.0	24.0	32.0
2	30.993460382367346	31.0	31.0	32.0	30.0	32.0
3	32.68207790713025	34.0	33.0	34.0	30.0	34.0
4	34.243311412930176	35.0	35.0	35.0	29.0	37.0
5	35.37426623560096	36.0	36.0	36.0	34.0	36.0
6	35.55077390026872	36.0	36.0	37.0	34.0	37.0
7	35.899040339370146	36.0	35.0	37.0	33.0	38.0
8	36.244114051010534	37.0	36.0	37.0	34.0	38.0
9	36.4800133322605	37.0	37.0	38.0	35.0	38.0
10-14	36.751367908755476	37.6	36.8	38.0	34.4	38.2
15-19	37.25066219478602	38.0	37.0	38.8	34.6	39.2
20-24	36.87634209554447	37.8	36.6	38.2	34.8	38.6
25-29	36.998549192852735	38.0	36.8	38.6	34.2	39.0
30-34	36.99302809833735	37.8	36.8	38.6	35.2	39.2
35-39	36.905853484149546	38.0	37.2	38.6	33.6	39.2
40-44	36.77715973737417	38.0	36.6	38.2	33.6	39.2
45-49	36.6350725245208	37.2	36.8	38.0	33.8	39.2
50-54	36.879878030526555	38.2	37.0	38.2	34.0	39.0
55-59	36.934325933841436	38.0	37.2	38.4	33.0	38.8
60-64	36.42861967574255	37.4	36.4	38.0	33.2	38.4
65-69	36.51498569998792	37.8	36.4	38.4	33.4	38.8
70-74	36.56427648493103	37.6	36.8	38.6	34.2	38.6
75-79	36.22974237243754	37.2	36.6	37.8	34.2	38.6
80-84	36.47352836473955	37.8	36.4	38.0	33.4	38.8
85-89	36.39021518739355	37.8	36.2	38.6	33.0	39.2
90-94	36.18671796910758	37.6	36.4	38.4	33.2	39.6
95-99	35.87709550265545	37.2	36.2	38.0	32.4	38.6
100	34.87805793087883	37.0	35.0	37.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	1.0
1	18.0
2	0.0
3	73.0
4	6.0
5	28.0
6	142.0
7	263.0
8	382.0
9	1336.0
10	706.0
11	1012.0
12	1158.0
13	1560.0
14	1879.0
15	2168.0
16	2652.0
17	3199.0
18	4187.0
19	4357.0
20	4301.0
21	5280.0
22	6526.0
23	8342.0
24	11090.0
25	14898.0
26	20612.0
27	28196.0
28	38224.0
29	51318.0
30	68123.0
31	91464.0
32	122470.0
33	167973.0
34	252902.0
35	488534.0
36	1617079.0
37	4483277.0
38	2016.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.90881946070388	15.746978121153399	20.56507372082446	39.77912869731826
2	20.2840180872012	45.52752116346428	18.583160357374105	15.605300391960414
3	21.570724722786327	31.22633712401172	29.957780500164926	17.245157653037026
4	34.96714577195313	23.02991863576222	20.244542990254303	21.758392602030348
5	22.732458490340324	26.04614413568068	24.732987094231902	26.488410279747093
6	22.618104464296064	27.44752559425743	25.02521491795943	24.909155023487077
7	23.07534395394134	26.26690828512132	26.03608477392098	24.62166298701636
8	23.141908746638084	25.87616317416343	27.045495328253207	23.93643275094528
9	22.88901349040051	27.62506099634761	25.840910335269097	23.645015177982778
10-14	23.416253151073413	25.999811631652225	25.80743346261121	24.77650175466315
15-19	22.58490422146502	26.23374608622506	26.66354653626355	24.517803156046366
20-24	22.950842956864467	25.208440443925724	26.612188463903724	25.22852813530608
25-29	22.791675519363235	26.25522353516344	27.428502104552045	23.52459884092128
30-34	22.65497500570575	27.17135774057646	26.750879277344218	23.422787976373566
35-39	22.99719992105246	26.29576515212394	27.676153031890728	23.03088189493287
40-44	22.64521247282911	27.203020268669842	27.035064964628603	23.116702293872443
45-49	23.050169760699337	27.29267844972918	27.24505812510593	22.41209366446555
50-54	22.98256053389412	27.092370386723623	27.21547089889639	22.709598180485866
55-59	22.714994594241357	27.595269823033096	26.985345407079564	22.704390175645976
60-64	22.64538244952473	27.147923782322703	27.741683791886402	22.465009976266167
65-69	23.094579203671994	27.54661039671929	27.44195719685486	21.916853202753856
70-74	22.640433140991874	27.60457781561943	27.045442842744123	22.70954620064457
75-79	23.089032568818315	27.717675244533034	26.896773744259363	22.296518442389285
80-84	22.425934682826714	27.64306882609539	26.972081184984326	22.95891530609357
85-89	22.851215528767625	27.590680210561608	27.075671031660402	22.482433229010365
90-94	22.748742467774623	26.98857098546345	27.38211389884181	22.880572647920122
95-99	22.77064732872794	26.740780316579393	27.37428003627762	23.114292318415046
100	23.417159714828895	26.153329416403594	26.623546238842554	23.805964629924954
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	63.68794815802213
2	35.889318479161616
3	38.81588237582336
4	56.725538373983476
5	49.220868770087414
6	47.527259487783134
7	47.6970069409577
8	47.07834149758337
9	46.53402866838329
10-14	48.192754905736564
15-19	47.102707377511386
20-24	48.179371092170555
25-29	46.31627436028452
30-34	46.07776298207932
35-39	46.02808181598533
40-44	45.76191476670156
45-49	45.46226342516489
50-54	45.692158714379985
55-59	45.41938476988733
60-64	45.1103924257909
65-69	45.01143240642585
70-74	45.34997934163644
75-79	45.3855510112076
80-84	45.38484998892029
85-89	45.333648757777986
90-94	45.62931511569474
95-99	45.884939647142986
100	47.22312434475385
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1036.0
1	1034.0
2	1034.5
3	1034.0
4	1034.5
5	1046.0
6	1053.5
7	1065.5
8	1090.5
9	1107.5
10	1129.0
11	1175.0
12	1204.5
13	1251.0
14	1308.0
15	1482.5
16	1634.5
17	1722.5
18	2128.5
19	2900.5
20	4104.5
21	5154.0
22	7543.5
23	12052.5
24	21168.0
25	32730.5
26	43479.0
27	52965.5
28	62137.0
29	79422.5
30	103893.5
31	120085.0
32	133994.0
33	151398.5
34	179491.0
35	196027.0
36	186393.5
37	185810.0
38	210875.0
39	246190.5
40	281829.5
41	280253.5
42	254816.0
43	289648.0
44	323934.0
45	313320.0
46	281027.5
47	241074.0
48	243757.5
49	260781.5
50	273415.0
51	293208.5
52	250169.0
53	184448.0
54	165827.0
55	152192.5
56	123240.0
57	102183.5
58	127237.0
59	132400.0
60	120795.5
61	128001.5
62	113763.5
63	93614.5
64	74268.5
65	71796.0
66	93220.5
67	87424.5
68	54823.5
69	41496.5
70	35298.5
71	29615.5
72	27062.0
73	17360.5
74	10822.5
75	12400.0
76	12227.0
77	11364.5
78	8666.5
79	7313.5
80	6728.0
81	5875.5
82	3966.5
83	2783.0
84	2708.0
85	1748.5
86	2917.5
87	2105.5
88	623.5
89	458.0
90	308.5
91	247.0
92	175.0
93	126.0
94	101.5
95	92.5
96	84.0
97	79.0
98	77.5
99	74.0
100	67.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03770769199621937
2	0.011296563020700952
3	0.0029706767561328822
4	0.002837463307802824
5	0.0013454706329719967
6	0.002437837800393571
7	1.4653747922498193E-4
8	0.0013188383671662
9	9.325122252352729E-5
10-14	3.863268021012981E-4
15-19	0.012112042951553
20-24	0.012003739304681602
25-29	0.008322529989422687
30-34	0.00949557875938971
35-39	0.00610326918015638
40-44	0.010362687119398466
45-49	0.00986750147186232
50-54	0.009877707522067776
55-59	0.00972625396520544
60-64	0.038623751807824576
65-69	0.029978987781318543
70-74	0.018373300804954754
75-79	0.03824732717382999
80-84	0.04477927205475061
85-89	0.012279876360232457
90-94	0.0023809937906904754
95-99	0.006252916550989116
100	0.01175510734479055
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
0-1	1044.0
2-3	3.0
4-5	53.0
6-7	46.0
8-9	2.0
10-11	8.0
12-13	0.0
14-15	6.0
16-17	11.0
18-19	57.0
20-21	677.0
22-23	168.0
24-25	670.0
26-27	147.0
28-29	139.0
30-31	223.0
32-33	107.0
34-35	126.0
36-37	151.0
38-39	135.0
40-41	156.0
42-43	102.0
44-45	208.0
46-47	200.0
48-49	157.0
50-51	12731.0
52-53	451.0
54-55	15646.0
56-57	24793.0
58-59	53112.0
60-61	1192.0
62-63	23401.0
64-65	13746.0
66-67	10509.0
68-69	13761.0
70-71	24175.0
72-73	27545.0
74-75	12503.0
76-77	9825.0
78-79	4143.0
80-81	8998.0
82-83	14106.0
84-85	9120.0
86-87	5073.0
88-89	7107.0
90-91	19483.0
92-93	2223.0
94-95	8039.0
96-97	163248.0
98-99	0.0
100-101	7018226.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	98.37388075684972
#Duplication Level	Relative count
1	100.0
2	13.020436581070566
3	4.650038907318671
4	2.252528975713642
5	1.1369128066511038
6	0.6487283450055289
7	0.4185608387598804
8	0.3088012450341975
9	0.19904165130851456
10++	7.925625588729164
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG	10347	0.13781755177848176	No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA	10237	0.13635239949321715	No Hit
GACAACTTGGCCACCGACAGCTGAAGGGCTTTTCACCTGTTGACACAATTGCCAGTCA	9250	0.12320598762452463	No Hit
TGACTGGCAATTGTGTCAACAGGTGAAAAGCCCTTCAGCTGTCGGTGGCCAAGTTGTC	9236	0.12301951369730912	No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC	8646	0.11516096962179892	No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG	8639	0.11506773265819116	No Hit
CATGCACTATCCAGGCGCCTTCACCTACTCCCCGACGCCGGTCACCTCGGGCATCGGCATCGG	8610	0.11468146523753049	No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT	8387	0.11171120196831223	No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT	8354	0.11127165628273283	No Hit
CCGATGCCGATGCCCGAGGTGACCGGCGTCGGGGAGTAGGTGAAGGCGCCTGGATAGTGCATG	8296	0.1104991214414115	No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA	8169	0.10880753653024235	No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG	8143	0.10846122780827071	No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT	8075	0.10755549730465258	No Hit
GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG	7980	0.10629013851283313	No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA	7873	0.10486494492625756	No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC	7851	0.10457191446920464	No Hit
GAGCTACCATCTGATCTGTCTGTCTTGACCACCCGGAGTCCCACTGTCCCCAGCCAGAATCCCAGTAGACTA	7718	0.10280041216065741	No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA	7666	0.10210779471671413	No Hit
CATGTCATCTCAAAGGTCCCCAGGATTCGAACACCCAGTTATTCTCCAAC	7635	0.10169488816359411	No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC	7634	0.10168156859736444	No Hit
AGCCACCTCACCCACCCGGAATCTACATTTTCAATCAGTAAAAGTCACCA	7626	0.101575012067527	No Hit
TAGTCTACTGGGATTCTGGCTGGGGACAGTGGGACTCCGGGTGGTCAAGACAGACAGATCAGATGGTAGCTC	7595	0.10116210551440698	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	3103535	3.1576824	4.746329	75-79
AAAAA	3071085	3.0091963	4.271723	2
CCCCA	1403880	2.51042	5.703453	1
CCAGC	1346990	2.4401426	5.415353	5
CCTCC	1316265	2.3715396	5.9131656	6
GGGGG	1061700	2.3545332	5.67865	4
TGGGG	1232205	2.3383403	7.8389425	3
CTGCC	1253565	2.2880647	5.2895684	7
CCACC	1173215	2.0979447	5.4835362	1
GGAAG	1295340	2.0719967	5.047251	4
CCCTG	1088030	1.9859227	5.0523577	1
TGGCT	1225155	1.9638264	6.235578	3
TGCAG	1222565	1.9449722	5.951864	3
GCCCA	1001435	1.8141519	5.622545	2
GGCTC	969505	1.7926931	7.171089	1
GCTCC	894325	1.6323633	6.848286	2
TGCCC	861490	1.5724313	9.325171	3
TAAAA	1588125	1.5678841	5.9693956	1
TGAGG	931905	1.5019226	5.0039477	1
GGCCC	696765	1.4862301	5.9447117	1
CATGG	929180	1.4782275	36.672607	1
ATGGA	1067405	1.4610204	10.108017	2
TGGCC	742855	1.3735989	5.103761	3
TGGGT	823555	1.3373317	5.8617315	3
ATGTG	948790	1.3084819	9.98032	2
ATGGG	797715	1.2856526	11.691531	2
ATGGC	792340	1.2605296	10.640399	2
TGAGA	898350	1.2296247	5.2908287	1
ATGAA	1032175	1.1998625	8.318507	2
ATGCC	737570	1.1582712	8.811735	2
TGAGC	705790	1.1228377	5.115534	1
ATGTT	930175	1.097701	6.465527	2
CATGA	805175	1.0878845	24.03675	1
CATGT	782290	1.0649544	27.775463	1
ATGCT	773785	1.0533762	8.322267	2
ATGAG	766605	1.0492976	5.487634	2
ATGGT	721595	0.995156	6.63181	2
CATGC	616600	0.96830136	28.487347	1
ATGAC	697185	0.9419777	6.508712	2
ATGTA	735335	0.86125946	6.5233502	2
ATGCA	612010	0.8268964	9.515828	2
TAAGT	632750	0.74110705	6.038148	1
>>END_MODULE