##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	8016-7-2.h.sapiens.aligned.bam	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	7433264	
Filtered Sequences	0	
Sequence length	1-100	
%GC	47	
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.831488831824082	24.0	24.0	32.0	24.0	32.0
2	31.010134941973135	31.0	31.0	32.0	30.0	32.0
3	32.7055118290318	34.0	33.0	34.0	30.0	34.0
4	34.27199168915927	35.0	35.0	35.0	29.0	37.0
5	35.39409665045817	36.0	36.0	36.0	34.0	36.0
6	35.57281659196246	36.0	36.0	37.0	34.0	37.0
7	35.92595203061792	36.0	35.0	37.0	33.0	38.0
8	36.2666157946561	37.0	36.0	37.0	34.0	38.0
9	36.50624892960922	37.0	37.0	38.0	35.0	38.0
10-14	36.77359295303013	37.6	36.8	38.0	34.4	38.2
15-19	37.27335282708574	38.0	37.0	38.8	34.6	39.2
20-24	36.89187589275626	37.8	36.6	38.2	35.2	38.6
25-29	37.012666496327704	38.0	36.8	38.6	34.2	39.0
30-34	37.00395821638074	37.8	36.8	38.6	35.4	39.2
35-39	36.90461385302309	38.0	37.2	38.4	33.6	39.2
40-44	36.773467916489366	38.0	36.6	38.2	33.6	39.2
45-49	36.640974949059874	37.2	36.8	38.0	33.8	39.2
50-54	36.885884721305175	38.2	37.0	38.2	34.0	39.0
55-59	36.94247290891214	38.0	37.2	38.4	33.0	38.8
60-64	36.437927805641216	37.4	36.4	38.0	33.4	38.4
65-69	36.52707095260121	37.8	36.4	38.4	33.8	38.8
70-74	36.57330996021841	37.6	36.8	38.6	34.2	38.6
75-79	36.23071748915246	37.2	36.6	37.8	34.2	38.6
80-84	36.47544930685131	37.8	36.4	38.0	33.4	38.8
85-89	36.39070719216106	37.8	36.2	38.6	33.0	39.2
90-94	36.18723954868721	37.6	36.4	38.4	33.2	39.6
95-99	35.872412789115515	37.2	36.2	38.0	32.4	38.6
100	34.85856696748052	37.0	35.0	37.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
1	1.0
2	0.0
3	2.0
4	0.0
5	27.0
6	121.0
7	206.0
8	303.0
9	515.0
10	608.0
11	893.0
12	1184.0
13	1400.0
14	1701.0
15	2161.0
16	2676.0
17	3272.0
18	4529.0
19	4552.0
20	4283.0
21	5032.0
22	6337.0
23	8030.0
24	10817.0
25	14508.0
26	20142.0
27	27522.0
28	37382.0
29	49643.0
30	65679.0
31	88867.0
32	120017.0
33	165199.0
34	248276.0
35	484386.0
36	1612807.0
37	4438183.0
38	2003.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.3373632338222	15.807267844556828	21.30041743990915	39.55495148171182
2	20.208245625861014	46.110268626039826	17.02938373551288	16.652102012586283
3	22.237891185374103	31.027089771723297	28.73642548257738	17.998593560325215
4	34.954873918195624	22.769002140737623	19.84677151150714	22.429352429559614
5	22.91107423971582	25.482228476278152	23.989555825252186	27.617141458753835
6	22.777502052022967	27.188883921490227	24.088038747682774	25.945575278804036
7	23.31384820705447	25.688697191720188	25.394604077481294	25.602850523744053
8	23.162488085139874	25.251579286541205	26.733441455573796	24.852491172745122
9	23.490194099979483	27.542339760729984	25.356015888551433	23.6114502507391
10-14	23.86027448044569	25.584502550102346	25.188019075700275	25.367203893751693
15-19	23.070214991829534	25.561009828994464	26.105329968772363	25.263445210403646
20-24	23.33355311422345	24.737570852425115	26.016480337325092	25.91239569602634
25-29	23.311261322410868	25.658306302225142	26.91991561191428	24.110516763449716
30-34	23.1251201907711	26.6424999949535	26.207787890960642	24.024591923314755
35-39	23.434455583816153	25.880513472908156	27.075980553578304	23.609050389697384
40-44	23.18036492790129	26.60315038865553	26.433213606430506	23.783271077012678
45-49	23.24996085534826	26.77221505755388	26.708703415273625	23.269120671824243
50-54	23.55103868969332	26.479876266836467	26.688372924214082	23.28071211925613
55-59	23.1693387466	27.102301950522513	26.48814338492702	23.24021591795046
60-64	23.254260524835637	26.474730245671196	27.221801669138472	23.049207560354695
65-69	23.77165271969986	26.89312397985147	26.89503077378028	22.440192526668387
70-74	23.214613057607732	27.060581387495464	26.496337984649642	23.22846757024716
75-79	23.61088389019307	27.24886253154153	26.30734588813537	22.832907690130035
80-84	23.01318527785223	27.20843681731336	26.324935819563866	23.45344208527055
85-89	23.479642541887014	26.98912506146992	26.552814026183384	22.97841837045968
90-94	23.369317763648194	26.506531202148846	26.63346012789802	23.490690906304945
95-99	23.361787639190716	26.096333246860908	26.782635002182637	23.75924411176574
100	24.45537631153384	25.51266669046161	25.804043994447014	24.227913003557536
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	62.89231471553403
2	36.860347638447294
3	40.23648474569932
4	57.38422634775524
5	50.52821569846966
6	48.723077330827
7	48.91669873079852
8	48.014979257884995
9	47.10164435071859
10-14	49.22747837419739
15-19	48.33366020223318
20-24	49.24594881024979
25-29	47.42177808586058
30-34	47.14971211408585
35-39	47.04350597351353
40-44	46.963636004913965
45-49	46.5190815271725
50-54	46.83175080894945
55-59	46.40955466455046
60-64	46.30346808519033
65-69	46.21184524636824
70-74	46.4430806278549
75-79	46.44379158032311
80-84	46.466627363122775
85-89	46.458060912346696
90-94	46.86000866995314
95-99	47.121031750956455
100	48.68328931509138
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	71.0
1	74.0
2	72.0
3	70.5
4	75.0
5	76.0
6	79.0
7	92.0
8	116.5
9	135.5
10	155.5
11	178.5
12	207.5
13	244.5
14	281.0
15	544.0
16	737.0
17	727.0
18	1053.0
19	2017.5
20	2919.0
21	3481.5
22	5583.0
23	10238.5
24	19962.5
25	29387.0
26	36865.0
27	45120.0
28	53230.0
29	70201.0
30	93821.5
31	106804.0
32	117133.5
33	132913.5
34	156040.5
35	169541.0
36	163064.5
37	165193.0
38	188986.0
39	221167.0
40	253707.0
41	258270.0
42	239269.5
43	261313.5
44	296623.0
45	297552.5
46	270436.0
47	236817.5
48	235691.5
49	257319.0
50	282184.0
51	301168.0
52	261863.5
53	198379.0
54	178230.0
55	169527.5
56	137104.5
57	110582.0
58	141920.5
59	150741.5
60	139872.5
61	150739.5
62	132487.0
63	109540.0
64	89034.0
65	87281.5
66	109981.0
67	100411.5
68	63746.5
69	47832.5
70	44251.5
71	38699.0
72	30892.0
73	18769.5
74	13423.5
75	16024.5
76	14582.0
77	13446.0
78	10767.5
79	8517.0
80	7966.0
81	6799.5
82	4205.0
83	3356.5
84	3166.5
85	1720.5
86	3134.0
87	2244.5
88	584.5
89	459.0
90	358.5
91	310.0
92	228.0
93	173.5
94	145.0
95	138.0
96	129.0
97	120.0
98	119.5
99	113.0
100	107.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03917525329384239
2	0.011004689100716449
3	0.003255665968671614
4	0.0031076861688996487
5	0.0012511542907327406
6	0.002475416357632011
7	1.748949587604414E-4
8	0.0011839043362245262
9	1.076276669295024E-4
10-14	3.6593455986884475E-4
15-19	0.01170186468635276
20-24	0.011301739768292808
25-29	0.008808040661984672
30-34	0.009852485603691208
35-39	0.006504828051854325
40-44	0.010143767517444105
45-49	0.009918083894016836
50-54	0.010398272156023364
55-59	0.010225351068805363
60-64	0.03818590493665254
65-69	0.02952178861986148
70-74	0.018186460471362107
75-79	0.03948711285113835
80-84	0.04519907518623105
85-89	0.012236984905826637
90-94	0.0025368229004753356
95-99	0.0066839771118714615
100	0.012656923821356478
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
0-1	69.0
2-3	12.0
4-5	90.0
6-7	60.0
8-9	2.0
10-11	10.0
12-13	0.0
14-15	6.0
16-17	17.0
18-19	30.0
20-21	650.0
22-23	134.0
24-25	281.0
26-27	106.0
28-29	91.0
30-31	87.0
32-33	64.0
34-35	101.0
36-37	84.0
38-39	88.0
40-41	138.0
42-43	86.0
44-45	106.0
46-47	173.0
48-49	88.0
50-51	16288.0
52-53	592.0
54-55	14591.0
56-57	27433.0
58-59	63188.0
60-61	847.0
62-63	28757.0
64-65	13332.0
66-67	10345.0
68-69	14448.0
70-71	23942.0
72-73	34623.0
74-75	10271.0
76-77	9924.0
78-79	4529.0
80-81	10490.0
82-83	16294.0
84-85	9191.0
86-87	4725.0
88-89	9876.0
90-91	18822.0
92-93	480.0
94-95	7811.0
96-97	158779.0
98-99	0.0
100-101	6921113.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	98.40085055501864
#Duplication Level	Relative count
1	100.0
2	13.435799072929022
3	4.473832538340526
4	2.0770764454988266
5	1.0305462315658414
6	0.6166452144798055
7	0.4214723771546829
8	0.2910767315279846
9	0.20442672185346894
10++	7.505741614718724
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG	11740	0.1579386928810816	No Hit
CATGCACTATCCAGGCGCCTTCACCTACTCCCCGACGCCGGTCACCTCGGGCATCGGCATCGG	11634	0.15651267061145682	No Hit
TGACTGGCAATTGTGTCAACAGGTGAAAAGCCCTTCAGCTGTCGGTGGCCAAGTTGTC	11626	0.156405046289221	No Hit
GACAACTTGGCCACCGACAGCTGAAGGGCTTTTCACCTGTTGACACAATTGCCAGTCA	11624	0.15637814020866203	No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA	11582	0.15581311251692392	No Hit
CCGATGCCGATGCCCGAGGTGACCGGCGTCGGGGAGTAGGTGAAGGCGCCTGGATAGTGCATG	11172	0.1502973660023376	No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC	10983	0.1477547413895161	No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG	10931	0.1470551832949832	No Hit
GAGCTACCATCTGATCTGTCTGTCTTGACCACCCGGAGTCCCACTGTCCCCAGCCAGAATCCCAGTAGACTA	10685	0.14374573538623142	No Hit
TAGTCTACTGGGATTCTGGCTGGGGACAGTGGGACTCCGGGTGGTCAAGACAGACAGATCAGATGGTAGCTC	10465	0.14078606652474607	No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT	10073	0.13551247473519035	No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT	10007	0.13462457407674475	No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT	9608	0.12925681100523267	No Hit
GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG	9563	0.12865142419265616	No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA	9106	0.12250338478493432	No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG	9095	0.12235540134186004	No Hit
ATTCAGCTGCCAAGTAACTTAGCACACCCATTCTTATAAGATAATGGCAA	8956	0.12048542874301249	No Hit
TAATGAGCATGAAGTCACCACACGGAGGAAAATGTAAATGTGTAAACCTC	8901	0.11974551152764115	No Hit
CATGGAGGGGAGCACTCAGCACCTCCCTCACCCCACACCCTTGGCTGCTC	8679	0.11675893658559684	No Hit
GCCTCAGCCTTCCTTCGCAGAAAGTTGAGCAGGTCGCCATAGCAACAGTA	8678	0.11674548354531737	No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA	8328	0.11203691944749977	No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC	8308	0.1117678586419102	No Hit
CATGTGCCTTCACTGTGTCCCAGGAAATCTGGGTTGGTTCCAGTGGGAAA	8234	0.11077233366122877	No Hit
CGGGGCTTGCTCTCCCCATCCCTATTTCCCCATCTCCTACTCTTTTGCTA	8166	0.1098575269222242	No Hit
CATGTCATCTCAAAGGTCCCCAGGATTCGAACACCCAGTTATTCTCCAAC	8100	0.10896962626377861	No Hit
AGCCACCTCACCCACCCGGAATCTACATTTTCAATCAGTAAAAGTCACCA	8092	0.10886200194154277	No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA	7918	0.10652117293291345	No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC	7878	0.1059830513217343	No Hit
CATGGCTCTGCTGTGCCTTCCATCCTGGGCTCCCTTCTCTCCTGTGACCT	7836	0.10541802362999618	No Hit
ACAGTGAAAACAGTGACAATGTTCCAGCAAAGCCACCAGACAAAGTTCTT	7827	0.10529694626748087	No Hit
TAGCTAAAAGGGGAAGAAGAGGATCAGCCCAAGGAGGAGGAAGAGGAAAA	7747	0.10422070304512258	No Hit
GCTCCGCAGGGATCGGGACACTGGACACACGTTCCTCTCCTCTGCACTGG	7746	0.10420725000484309	No Hit
CATGGGGTGGGTGCTTGCAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTT	7661	0.10306374158108739	No Hit
GGCTGCTTGTTGTACGTGGCCTGGTGGAACGCACTGCAAAACGAGCTCAG	7569	0.10182606187537534	No Hit
ACCTATGTTACACCATCTTCAGTGCCAGTCTTGGGCAAAATTGTGCAAGA	7551	0.10158390715034472	No Hit
GAAGGGCAGGAGTTCCTTTGGTGACTTCACAGTGAAGTCTTGCCCTCTCT	7488	0.10073636561273756	No Hit
TAAAACTGCATCAAGTCATGGGGCATGTGGAAGGTAGGGAGGCAAGATGA	7451	0.10023860312239684	No Hit
TAGCTGCTCCATTTTCATTTCACCACTTTGAAATCTTGGAACCACTGATG	7449	0.10021169704183788	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	2800685	3.2153037	4.8964195	75-79
AAAAA	2803005	3.0590208	4.9179077	2
CCCAG	1725135	2.9201365	5.8121867	2
CCCCA	1521345	2.5297625	6.15929	1
CCAGC	1436210	2.4310732	5.081019	5
CCTCC	1399175	2.3503034	5.854129	6
TGGGG	1299205	2.3433747	8.30092	3
CTGCC	1331950	2.2775497	5.317426	7
GGGGG	1120005	2.261703	6.325815	4
GGAAG	1326245	2.0938065	5.005905	4
CCACC	1250405	2.079231	5.8914948	1
CTCCA	1368735	2.0694165	5.036469	6
TGGCT	1257175	1.9896661	6.6044827	3
TGCAG	1237430	1.9386761	5.040995	3
CCCTG	1129600	1.9315441	5.3178015	1
GCCCA	1071370	1.8135084	6.167807	2
GGCTC	1039270	1.8089908	8.338172	1
GCTCC	954100	1.6314502	8.378791	2
TAAAA	1444835	1.5928564	6.6353	1
GATGG	995240	1.5872321	5.088604	1
TGCCC	925125	1.5819049	10.504114	3
CATGG	941095	1.4744093	36.14182	1
TGAGG	914275	1.4581075	5.3228493	1
ATGGA	1026595	1.4476393	10.2438	2
GGCCC	744110	1.4245204	5.924723	1
ATGGG	846850	1.3505764	12.197577	2
TGGGT	828325	1.3344836	5.7214437	3
TGCTC	840095	1.3061252	5.579679	1
ATGTG	914705	1.302993	9.773773	2
ATGGC	818785	1.282787	11.2035885	2
TGAGA	869075	1.2255145	5.1200123	1
ATGAA	960735	1.1978803	7.8296075	2
ATGCC	755265	1.162401	10.095546	2
TGAGC	721860	1.130935	5.0166144	1
ATGTT	873650	1.1115961	6.045414	2
CATGA	778470	1.078388	21.541786	1
CATGT	755020	1.0565534	27.050901	1
ATGCT	746355	1.044428	8.292622	2
ATGGT	690660	0.9838421	6.1730866	2
CATGC	633440	0.9749044	28.511227	1
ATGAC	676355	0.93693155	5.8618197	2
ATGTA	685285	0.86313933	6.896658	2
TAATG	640635	0.80690116	5.5671835	1
ATGCA	571670	0.79191506	9.250859	2
ATGTC	542375	0.75898415	5.0844502	2
TAAGT	594870	0.7492586	6.5599847	1
>>END_MODULE