FastQCFastQC Report
Wed 20 Mar 2013
8016-7-2.h.sapiens.aligned.bam

Summary

[OK] Basic Statistics

Measure Value
Filename 8016-7-2.h.sapiens.aligned.bam
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 7433264
Filtered Sequences 0
Sequence length 1-100
%GC 47

[WARN] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG 11740 0.1579386928810816 No Hit
CATGCACTATCCAGGCGCCTTCACCTACTCCCCGACGCCGGTCACCTCGGGCATCGGCATCGG 11634 0.15651267061145682 No Hit
TGACTGGCAATTGTGTCAACAGGTGAAAAGCCCTTCAGCTGTCGGTGGCCAAGTTGTC 11626 0.156405046289221 No Hit
GACAACTTGGCCACCGACAGCTGAAGGGCTTTTCACCTGTTGACACAATTGCCAGTCA 11624 0.15637814020866203 No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA 11582 0.15581311251692392 No Hit
CCGATGCCGATGCCCGAGGTGACCGGCGTCGGGGAGTAGGTGAAGGCGCCTGGATAGTGCATG 11172 0.1502973660023376 No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC 10983 0.1477547413895161 No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG 10931 0.1470551832949832 No Hit
GAGCTACCATCTGATCTGTCTGTCTTGACCACCCGGAGTCCCACTGTCCCCAGCCAGAATCCCAGTAGACTA 10685 0.14374573538623142 No Hit
TAGTCTACTGGGATTCTGGCTGGGGACAGTGGGACTCCGGGTGGTCAAGACAGACAGATCAGATGGTAGCTC 10465 0.14078606652474607 No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT 10073 0.13551247473519035 No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT 10007 0.13462457407674475 No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT 9608 0.12925681100523267 No Hit
GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG 9563 0.12865142419265616 No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA 9106 0.12250338478493432 No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG 9095 0.12235540134186004 No Hit
ATTCAGCTGCCAAGTAACTTAGCACACCCATTCTTATAAGATAATGGCAA 8956 0.12048542874301249 No Hit
TAATGAGCATGAAGTCACCACACGGAGGAAAATGTAAATGTGTAAACCTC 8901 0.11974551152764115 No Hit
CATGGAGGGGAGCACTCAGCACCTCCCTCACCCCACACCCTTGGCTGCTC 8679 0.11675893658559684 No Hit
GCCTCAGCCTTCCTTCGCAGAAAGTTGAGCAGGTCGCCATAGCAACAGTA 8678 0.11674548354531737 No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA 8328 0.11203691944749977 No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC 8308 0.1117678586419102 No Hit
CATGTGCCTTCACTGTGTCCCAGGAAATCTGGGTTGGTTCCAGTGGGAAA 8234 0.11077233366122877 No Hit
CGGGGCTTGCTCTCCCCATCCCTATTTCCCCATCTCCTACTCTTTTGCTA 8166 0.1098575269222242 No Hit
CATGTCATCTCAAAGGTCCCCAGGATTCGAACACCCAGTTATTCTCCAAC 8100 0.10896962626377861 No Hit
AGCCACCTCACCCACCCGGAATCTACATTTTCAATCAGTAAAAGTCACCA 8092 0.10886200194154277 No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA 7918 0.10652117293291345 No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC 7878 0.1059830513217343 No Hit
CATGGCTCTGCTGTGCCTTCCATCCTGGGCTCCCTTCTCTCCTGTGACCT 7836 0.10541802362999618 No Hit
ACAGTGAAAACAGTGACAATGTTCCAGCAAAGCCACCAGACAAAGTTCTT 7827 0.10529694626748087 No Hit
TAGCTAAAAGGGGAAGAAGAGGATCAGCCCAAGGAGGAGGAAGAGGAAAA 7747 0.10422070304512258 No Hit
GCTCCGCAGGGATCGGGACACTGGACACACGTTCCTCTCCTCTGCACTGG 7746 0.10420725000484309 No Hit
CATGGGGTGGGTGCTTGCAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTT 7661 0.10306374158108739 No Hit
GGCTGCTTGTTGTACGTGGCCTGGTGGAACGCACTGCAAAACGAGCTCAG 7569 0.10182606187537534 No Hit
ACCTATGTTACACCATCTTCAGTGCCAGTCTTGGGCAAAATTGTGCAAGA 7551 0.10158390715034472 No Hit
GAAGGGCAGGAGTTCCTTTGGTGACTTCACAGTGAAGTCTTGCCCTCTCT 7488 0.10073636561273756 No Hit
TAAAACTGCATCAAGTCATGGGGCATGTGGAAGGTAGGGAGGCAAGATGA 7451 0.10023860312239684 No Hit
TAGCTGCTCCATTTTCATTTCACCACTTTGAAATCTTGGAACCACTGATG 7449 0.10021169704183788 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 2800685 3.2153037 4.8964195 75-79
AAAAA 2803005 3.0590208 4.9179077 2
CCCAG 1725135 2.9201365 5.8121867 2
CCCCA 1521345 2.5297625 6.15929 1
CCAGC 1436210 2.4310732 5.081019 5
CCTCC 1399175 2.3503034 5.854129 6
TGGGG 1299205 2.3433747 8.30092 3
CTGCC 1331950 2.2775497 5.317426 7
GGGGG 1120005 2.261703 6.325815 4
GGAAG 1326245 2.0938065 5.005905 4
CCACC 1250405 2.079231 5.8914948 1
CTCCA 1368735 2.0694165 5.036469 6
TGGCT 1257175 1.9896661 6.6044827 3
TGCAG 1237430 1.9386761 5.040995 3
CCCTG 1129600 1.9315441 5.3178015 1
GCCCA 1071370 1.8135084 6.167807 2
GGCTC 1039270 1.8089908 8.338172 1
GCTCC 954100 1.6314502 8.378791 2
TAAAA 1444835 1.5928564 6.6353 1
GATGG 995240 1.5872321 5.088604 1
TGCCC 925125 1.5819049 10.504114 3
CATGG 941095 1.4744093 36.14182 1
TGAGG 914275 1.4581075 5.3228493 1
ATGGA 1026595 1.4476393 10.2438 2
GGCCC 744110 1.4245204 5.924723 1
ATGGG 846850 1.3505764 12.197577 2
TGGGT 828325 1.3344836 5.7214437 3
TGCTC 840095 1.3061252 5.579679 1
ATGTG 914705 1.302993 9.773773 2
ATGGC 818785 1.282787 11.2035885 2
TGAGA 869075 1.2255145 5.1200123 1
ATGAA 960735 1.1978803 7.8296075 2
ATGCC 755265 1.162401 10.095546 2
TGAGC 721860 1.130935 5.0166144 1
ATGTT 873650 1.1115961 6.045414 2
CATGA 778470 1.078388 21.541786 1
CATGT 755020 1.0565534 27.050901 1
ATGCT 746355 1.044428 8.292622 2
ATGGT 690660 0.9838421 6.1730866 2
CATGC 633440 0.9749044 28.511227 1
ATGAC 676355 0.93693155 5.8618197 2
ATGTA 685285 0.86313933 6.896658 2
TAATG 640635 0.80690116 5.5671835 1
ATGCA 571670 0.79191506 9.250859 2
ATGTC 542375 0.75898415 5.0844502 2
TAAGT 594870 0.7492586 6.5599847 1
Produced by FastQC (version 0.10.0)