##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	8016-7-25.h.sapiens.aligned.bam	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	12017720	
Filtered Sequences	0	
Sequence length	1-100	
%GC	44	
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.816265481305937	24.0	24.0	32.0	24.0	32.0
2	31.011648899415658	31.0	31.0	32.0	30.0	32.0
3	32.68741437569648	34.0	33.0	34.0	30.0	34.0
4	34.25831820554093	35.0	35.0	35.0	29.0	37.0
5	35.38322774350759	36.0	36.0	36.0	34.0	36.0
6	35.571007936887014	36.0	36.0	37.0	34.0	37.0
7	35.9174131105234	37.0	35.0	37.0	33.0	38.0
8	36.267556322065	37.0	36.0	37.0	34.0	38.0
9	36.50625203036629	37.0	37.0	38.0	35.0	38.0
10-14	36.780945569211475	37.6	36.8	38.0	34.6	38.2
15-19	37.282862431962414	38.0	37.0	38.8	34.8	39.2
20-24	36.91477750496371	37.8	36.8	38.2	35.6	38.6
25-29	37.0497154486215	38.0	36.8	38.6	34.2	39.0
30-34	37.05085603973057	37.8	36.8	38.8	35.4	39.2
35-39	36.96989584104115	38.0	37.2	38.6	33.8	39.2
40-44	36.84881343163296	38.0	36.8	38.2	33.6	39.2
45-49	36.701796309409985	37.4	37.0	38.4	33.8	39.2
50-54	36.9457778653192	38.2	37.2	38.2	34.0	39.0
55-59	37.005408051157325	38.0	37.2	38.4	34.0	38.8
60-64	36.48412373708835	37.4	36.4	38.0	33.4	38.4
65-69	36.58047787452364	37.8	36.4	38.4	34.0	38.8
70-74	36.63398677391619	37.6	36.8	38.6	34.2	38.6
75-79	36.297236465053615	37.2	36.8	37.8	34.4	38.6
80-84	36.54708177419785	37.8	36.4	38.0	34.0	38.8
85-89	36.48695580060029	38.0	36.2	38.6	33.0	39.2
90-94	36.29337716770747	37.6	36.4	38.4	33.2	39.6
95-99	35.99638352575879	37.2	36.4	38.0	32.6	38.6
100	35.01665613548907	37.0	35.0	37.0	33.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	4.0
4	0.0
5	30.0
6	195.0
7	359.0
8	501.0
9	816.0
10	1015.0
11	1333.0
12	1789.0
13	2317.0
14	2764.0
15	3376.0
16	4077.0
17	5023.0
18	6294.0
19	6796.0
20	7078.0
21	8149.0
22	10305.0
23	12896.0
24	16784.0
25	22600.0
26	31198.0
27	42759.0
28	58020.0
29	77679.0
30	103611.0
31	137666.0
32	183242.0
33	251312.0
34	378822.0
35	724429.0
36	2451009.0
37	7459548.0
38	3924.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.469614749309144	17.494156114264044	21.81036133989368	38.22586779653314
2	19.418631755644817	47.50247932467499	18.651695099113486	14.427193820566714
3	19.890534333714672	33.967866616117256	30.285537064781025	15.856061985387049
4	33.31101541534045	24.58432669703967	21.097563918533773	21.007093969086103
5	21.684451527119233	27.46652757981302	26.102092868267768	24.746928024799978
6	21.696185714075302	28.72083499171068	25.962429566983978	23.62054972723004
7	22.054439365469587	27.867578635420276	26.89394156879495	23.184040430315182
8	22.07672673344871	26.947696349011206	28.117042269202614	22.858534648337468
9	21.504565163629273	28.856465703607032	26.9152851017996	22.723684030964094
10-14	22.29103054435566	27.291231085667423	26.983257879318067	23.434480490658853
15-19	21.464635805363656	27.618177311423597	27.77811381419023	23.139073069022515
20-24	21.765263453932164	26.532610607667213	28.066790847966484	23.635335090434143
25-29	21.796455207049238	27.263601406039285	28.40915090687137	22.530792480040105
30-34	22.024088274625214	27.86262820236891	27.65341730332782	22.45986621967805
35-39	22.247308285612647	27.068728308640015	28.552286335173093	22.13167707057424
40-44	21.89514541968141	27.65119850772989	28.03131223739559	22.42234383519311
45-49	22.222142875168352	28.23729321297706	27.96192286920367	21.57864104265092
50-54	22.205423863916995	27.85777349712391	27.778580919011326	22.158221719947772
55-59	22.216724774366593	28.195971662990654	27.63297693352079	21.954326629121958
60-64	22.001246336625094	27.94889530044416	28.43382722508641	21.616031137844335
65-69	22.19265808997055	28.316740168801367	28.203717220676687	21.286884520551396
70-74	21.977441091182875	28.332767812331955	27.699877236120418	21.989913860364755
75-79	22.388544659557898	28.270312051069002	27.692012621400142	21.64913066797296
80-84	21.72345096657012	28.463027893519495	27.835182699424443	21.97833844048594
85-89	21.963690951128104	28.37219535119483	27.853648359061577	21.810465338615487
90-94	21.992788322334125	27.727325979926583	28.27838409452167	22.001501603217623
95-99	21.88378727883921	27.485604577461594	28.30221558894123	22.328392554757965
100	22.64269808122611	26.985259200297463	27.44663360453709	22.92540911393933
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	60.695482545842275
2	33.84582557621153
3	35.74659631910172
4	54.31810938442655
5	46.431379551919214
6	45.31673544130534
7	45.23847979578477
8	44.93526138178618
9	44.22824919459337
10-14	45.72551103501451
15-19	44.60370887438617
20-24	45.400598544366304
25-29	44.32724768708935
30-34	44.48395449430326
35-39	44.37898535618688
40-44	44.31748925487452
45-49	43.80078391781927
50-54	44.363645583864766
55-59	44.171051403488555
60-64	43.61727747446943
65-69	43.479542610521946
70-74	43.96735495154763
75-79	44.037675327530856
80-84	43.70178940705606
85-89	43.774156289743594
90-94	43.99428992555175
95-99	44.212179833597176
100	45.568107195165446
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	116.0
1	115.0
2	116.5
3	119.0
4	125.0
5	141.0
6	158.5
7	180.0
8	230.0
9	269.5
10	299.0
11	370.5
12	455.5
13	588.5
14	657.5
15	1016.5
16	1241.5
17	1254.0
18	2190.0
19	3071.0
20	5718.0
21	8080.5
22	13319.0
23	21897.5
24	36044.0
25	61841.0
26	88977.5
27	109618.0
28	130122.5
29	176131.5
30	233044.5
31	248459.0
32	261746.0
33	302143.0
34	363293.5
35	391158.5
36	364090.5
37	352031.5
38	398322.5
39	450944.5
40	494869.5
41	489340.0
42	445394.0
43	481087.5
44	513089.5
45	490462.0
46	430289.5
47	363757.5
48	363673.5
49	375967.0
50	387047.5
51	411928.0
52	343795.0
53	253155.5
54	228570.5
55	204544.0
56	166650.0
57	140000.5
58	168847.5
59	173581.0
60	156654.0
61	167277.0
62	149436.5
63	120926.5
64	92718.0
65	86601.5
66	110597.0
67	102334.5
68	63125.5
69	49112.0
70	40644.0
71	32829.0
72	30750.0
73	18601.0
74	10448.5
75	12315.0
76	13246.0
77	12382.5
78	9037.5
79	7995.0
80	7240.5
81	7432.0
82	5430.0
83	3030.5
84	2945.5
85	1445.5
86	2922.5
87	2308.0
88	415.0
89	343.0
90	250.5
91	196.0
92	142.0
93	108.0
94	89.5
95	79.0
96	77.5
97	64.5
98	55.5
99	54.5
100	50.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.038709505630019674
2	0.010309865118006767
3	0.0027626111534933386
4	0.0030455291631884397
5	0.0011732819241690418
6	0.002404814688641403
7	1.6642345265922625E-4
8	0.0011233583054497773
9	3.328469330152204E-5
10-14	3.428329343823089E-4
15-19	0.011628244668453148
20-24	0.011408912102895172
25-29	0.008719572764568423
30-34	0.009916339251047757
35-39	0.006427889383713206
40-44	0.010159614636362942
45-49	0.009851935840478733
50-54	0.01014122269341607
55-59	0.009952383348719519
60-64	0.03771838371838129
65-69	0.029171180698788882
70-74	0.01787091239160392
75-79	0.03840808302017582
80-84	0.04442125653242861
85-89	0.01210072019294213
90-94	0.0025783001881898525
95-99	0.006789018776214574
100	0.012169559191423415
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
0-1	104.0
2-3	0.0
4-5	58.0
6-7	21.0
8-9	1.0
10-11	26.0
12-13	20.0
14-15	188.0
16-17	13.0
18-19	36.0
20-21	462.0
22-23	33.0
24-25	106.0
26-27	17.0
28-29	38.0
30-31	74.0
32-33	55.0
34-35	62.0
36-37	52.0
38-39	40.0
40-41	126.0
42-43	97.0
44-45	122.0
46-47	129.0
48-49	144.0
50-51	22506.0
52-53	1219.0
54-55	28809.0
56-57	36046.0
58-59	71794.0
60-61	2643.0
62-63	34793.0
64-65	19913.0
66-67	18145.0
68-69	20731.0
70-71	46734.0
72-73	41728.0
74-75	25716.0
76-77	24690.0
78-79	9864.0
80-81	21103.0
82-83	27567.0
84-85	12734.0
86-87	6524.0
88-89	7900.0
90-91	33688.0
92-93	8342.0
94-95	12066.0
96-97	280364.0
98-99	0.0
100-101	1.1200077E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	98.55255771740916
#Duplication Level	Relative count
1	100.0
2	12.840756078351667
3	4.076370010924519
4	1.7739640761159523
5	0.9666929193872107
6	0.592591651634867
7	0.37537156068189326
8	0.2769238586418028
9	0.21722009095297376
10++	5.909402708264526
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CATGCTTTCCCTGGGTCTTCACGGATTGCTTTCCAAGCTGCCTTGTTGCG	13443	0.11185982033197645	No Hit
GCGACAGCTGGAAAGGTCCAGTTGCTGCTCTGCAGCAACCCCGCAACAAG	13387	0.11139384176033391	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	5587200	2.8943539	5.1726093	2
CCCCA	1977340	2.5172427	5.6406527	1
GGGGG	1490640	2.4986365	6.221207	4
CCTCC	1895735	2.434464	6.283842	6
CCAGC	1859145	2.390727	5.2388415	5
TGGGG	1756850	2.3488343	8.316749	3
CCACC	1662955	2.1170168	5.075805	1
TGGCT	1812325	1.9132344	5.2348447	3
TGCAG	1796395	1.8799746	6.3977566	3
GCCCA	1412270	1.8160781	5.613486	2
GGCTC	1346050	1.7637334	6.8581696	1
GCTCC	1226070	1.590428	6.212158	2
TGCCC	1218700	1.5808678	8.33116	3
GGCCC	947285	1.5406076	5.9813175	1
CATGG	1450830	1.5183316	36.795895	1
TAAAA	2866850	1.4981122	7.0831246	1
ATGGA	1766600	1.476613	10.103628	2
TGGCC	1088305	1.4260093	5.391006	3
TGGGT	1230920	1.3126075	5.6024284	3
ATGTG	1550095	1.3069795	8.894675	2
ATGGC	1222525	1.2794043	9.343188	2
ATGGG	1185450	1.2531594	11.318693	2
ATGAA	1878040	1.2411915	7.7100706	2
TGAGA	1462190	1.2221721	5.3443384	1
ATGCC	1109425	1.1494102	7.235652	2
CATGT	1318120	1.1002527	27.584404	1
CATGA	1315655	1.0886734	23.646336	1
ATGCT	1274750	1.0640512	7.7841015	2
ATGTT	1578030	1.0612388	6.577054	2
ATGGT	1234655	1.0410129	6.3737864	2
ATGAC	1172430	0.9701579	6.186356	2
CATGC	932595	0.9662071	27.77939	1
ATGTA	1374140	0.9161084	6.327419	2
ATGCA	1047445	0.8667358	9.384981	2
TAAGT	1109690	0.7398056	6.3165793	1
TAAGG	824040	0.6887741	5.192908	1
>>END_MODULE