FastQCFastQC Report
Wed 20 Mar 2013
8016-7-25.h.sapiens.aligned.bam

Summary

[OK] Basic Statistics

Measure Value
Filename 8016-7-25.h.sapiens.aligned.bam
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 12017720
Filtered Sequences 0
Sequence length 1-100
%GC 44

[WARN] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
CATGCTTTCCCTGGGTCTTCACGGATTGCTTTCCAAGCTGCCTTGTTGCG 13443 0.11185982033197645 No Hit
GCGACAGCTGGAAAGGTCCAGTTGCTGCTCTGCAGCAACCCCGCAACAAG 13387 0.11139384176033391 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 5587200 2.8943539 5.1726093 2
CCCCA 1977340 2.5172427 5.6406527 1
GGGGG 1490640 2.4986365 6.221207 4
CCTCC 1895735 2.434464 6.283842 6
CCAGC 1859145 2.390727 5.2388415 5
TGGGG 1756850 2.3488343 8.316749 3
CCACC 1662955 2.1170168 5.075805 1
TGGCT 1812325 1.9132344 5.2348447 3
TGCAG 1796395 1.8799746 6.3977566 3
GCCCA 1412270 1.8160781 5.613486 2
GGCTC 1346050 1.7637334 6.8581696 1
GCTCC 1226070 1.590428 6.212158 2
TGCCC 1218700 1.5808678 8.33116 3
GGCCC 947285 1.5406076 5.9813175 1
CATGG 1450830 1.5183316 36.795895 1
TAAAA 2866850 1.4981122 7.0831246 1
ATGGA 1766600 1.476613 10.103628 2
TGGCC 1088305 1.4260093 5.391006 3
TGGGT 1230920 1.3126075 5.6024284 3
ATGTG 1550095 1.3069795 8.894675 2
ATGGC 1222525 1.2794043 9.343188 2
ATGGG 1185450 1.2531594 11.318693 2
ATGAA 1878040 1.2411915 7.7100706 2
TGAGA 1462190 1.2221721 5.3443384 1
ATGCC 1109425 1.1494102 7.235652 2
CATGT 1318120 1.1002527 27.584404 1
CATGA 1315655 1.0886734 23.646336 1
ATGCT 1274750 1.0640512 7.7841015 2
ATGTT 1578030 1.0612388 6.577054 2
ATGGT 1234655 1.0410129 6.3737864 2
ATGAC 1172430 0.9701579 6.186356 2
CATGC 932595 0.9662071 27.77939 1
ATGTA 1374140 0.9161084 6.327419 2
ATGCA 1047445 0.8667358 9.384981 2
TAAGT 1109690 0.7398056 6.3165793 1
TAAGG 824040 0.6887741 5.192908 1