##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	8016-7-3.h.sapiens.aligned.bam	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	8010440	
Filtered Sequences	0	
Sequence length	1-100	
%GC	46	
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.770739435037278	24.0	24.0	32.0	24.0	32.0
2	30.985673580297835	31.0	31.0	32.0	30.0	32.0
3	32.667847457842946	34.0	33.0	34.0	30.0	34.0
4	34.22232147115127	35.0	35.0	35.0	29.0	37.0
5	35.366962146128444	36.0	36.0	36.0	34.0	36.0
6	35.54087734362496	36.0	36.0	37.0	34.0	37.0
7	35.89780629236218	36.0	35.0	37.0	33.0	38.0
8	36.24039667469404	37.0	36.0	37.0	34.0	38.0
9	36.468819552953505	37.0	36.0	38.0	35.0	38.0
10-14	36.745692433668985	37.6	36.8	38.0	34.4	38.2
15-19	37.24741656444641	38.0	37.0	38.8	34.6	39.2
20-24	36.872532932612955	37.8	36.6	38.2	34.8	38.6
25-29	36.99076321794992	38.0	36.8	38.6	34.2	39.0
30-34	36.990499079208035	37.8	36.8	38.6	35.2	39.2
35-39	36.89716814145677	38.0	37.2	38.4	33.6	39.2
40-44	36.774389961420816	38.0	36.6	38.2	33.6	39.2
45-49	36.635684960788105	37.2	36.8	38.0	33.8	39.2
50-54	36.88464035528049	38.2	37.2	38.2	34.0	39.0
55-59	36.94062302684323	38.0	37.2	38.4	33.0	38.8
60-64	36.434849612232355	37.4	36.4	38.0	33.2	38.4
65-69	36.52372256911574	37.8	36.4	38.4	33.6	38.8
70-74	36.57604970185883	37.6	36.8	38.6	34.2	38.6
75-79	36.24477666409332	37.2	36.6	37.8	34.2	38.6
80-84	36.491253950317514	37.8	36.4	38.0	33.4	38.8
85-89	36.41307816999107	37.8	36.2	38.6	33.0	39.2
90-94	36.21406448088511	37.6	36.4	38.4	33.2	39.6
95-99	35.90361572517058	37.2	36.2	38.0	32.4	38.6
100	34.90954938358846	37.0	35.0	37.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
1	6.0
2	0.0
3	16.0
4	2.0
5	14.0
6	86.0
7	150.0
8	234.0
9	610.0
10	549.0
11	862.0
12	1068.0
13	1446.0
14	1806.0
15	2319.0
16	2757.0
17	3319.0
18	4640.0
19	4701.0
20	4505.0
21	5377.0
22	6664.0
23	8689.0
24	11426.0
25	15690.0
26	21747.0
27	29810.0
28	40785.0
29	54808.0
30	73033.0
31	97436.0
32	131307.0
33	178580.0
34	272622.0
35	527366.0
36	1740587.0
37	4763236.0
38	2187.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.17994451456237	16.24963501352417	21.494812258949693	39.07560821296377
2	19.918169922889447	46.6796141475888	17.08953863518319	16.312677294338567
3	21.325295428821548	31.814513622048363	29.25652337949548	17.60366756963461
4	35.05560798365924	22.847082820271037	20.18534288333541	21.91196631273431
5	22.572962467242686	25.768450930912607	24.723562657528806	26.935023944315905
6	22.453058467191457	27.78500285458852	24.890089290630073	24.87184938758995
7	23.196714537219005	26.00394203599118	26.130969576560716	24.6683738502291
8	22.87041489824424	25.65147608653422	27.19027201620382	24.287836999017724
9	23.09663190503662	27.778983897408228	25.538418669383578	23.585965528171577
10-14	23.42361633441541	26.02279858094134	25.64646349437551	24.907121590267735
15-19	22.608478442554347	26.1661494379339	26.50399540841925	24.721376711092503
20-24	22.925905829168176	25.24370611501866	26.434998683425587	25.395389372387577
25-29	22.869557955122488	26.08820667874986	27.40276594105902	23.639469425068636
30-34	22.71761576494893	27.0284737102532	26.724226662439634	23.52968386235824
35-39	22.962746203082332	26.381870325691857	27.615769285756176	23.039614185469635
40-44	22.691848119867405	27.04284580305242	26.984678767318442	23.280627309761734
45-49	23.0255369556459	27.248350995824595	27.215965786031255	22.51014626249825
50-54	23.10501457812481	27.06999724737054	27.037933706502027	22.787054468002616
55-59	22.783356868474463	27.473224780357096	26.980682092983876	22.76273625818456
60-64	22.75806331170397	26.975770851920046	27.706020187188134	22.56014564918785
65-69	23.066140152157804	27.569489263563657	27.442834642665083	21.921535941613463
70-74	22.593046413929972	27.609334083905495	27.036243879341406	22.761375622823127
75-79	23.076918256154055	27.690936745944473	26.99546428506054	22.23668071284093
80-84	22.570160447079637	27.609925462191075	26.950636864465906	22.869277226263378
85-89	22.953180456734238	27.508212734639326	27.014883249229694	22.523723559396746
90-94	22.779438464710836	26.883394447022912	27.382854727434353	22.9543123608319
95-99	22.915775451625045	26.673086844643763	27.235402502265927	23.175735201465265
100	23.977614360169973	26.052598265498776	26.24026450094334	23.72952287338791
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	62.255552727526144
2	36.23084721722802
3	38.92896299845616
4	56.96757429639355
5	49.50798641155859
6	47.32490785478141
7	47.865088387448104
8	47.15825189726196
9	46.682597433208194
10-14	48.33073792468314
15-19	47.32985515364685
20-24	48.321295201555756
25-29	46.50902738019112
30-34	46.24729962730717
35-39	46.00236038855196
40-44	45.97247542962914
45-49	45.53568321814415
50-54	45.892069046127425
55-59	45.546093126659024
60-64	45.31820896089182
65-69	44.98767609377126
70-74	45.3544220367531
75-79	45.31359896899498
80-84	45.43943767334302
85-89	45.47690401613099
90-94	45.733750825542735
95-99	46.09151065309031
100	47.70713723355788
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	272.0
1	270.0
2	270.0
3	270.0
4	270.5
5	278.5
6	287.5
7	296.5
8	317.5
9	341.5
10	364.0
11	391.5
12	413.5
13	473.5
14	529.5
15	726.0
16	902.5
17	1009.5
18	1317.5
19	1943.5
20	3073.0
21	4028.0
22	6609.5
23	11677.5
24	22320.0
25	33147.5
26	43444.5
27	55156.5
28	63440.5
29	80771.5
30	107215.5
31	123180.0
32	137239.0
33	156662.0
34	186612.5
35	201617.0
36	191989.5
37	192189.0
38	220555.0
39	260996.5
40	297394.5
41	302141.0
42	284038.0
43	313579.5
44	347543.5
45	342663.0
46	308404.0
47	254777.5
48	253461.0
49	283223.5
50	303397.0
51	324201.0
52	272655.0
53	197106.5
54	180680.5
55	165500.0
56	131210.0
57	106931.5
58	137082.0
59	148434.0
60	131864.5
61	138753.5
62	121289.5
63	97899.5
64	79896.5
65	80482.5
66	106267.0
67	100935.5
68	62106.0
69	44011.0
70	37312.5
71	30283.5
72	26896.5
73	17036.5
74	11114.0
75	13556.0
76	13569.0
77	12010.0
78	8506.5
79	7126.0
80	6650.5
81	5705.5
82	3808.5
83	2734.0
84	2482.5
85	1583.0
86	2913.0
87	2056.0
88	556.5
89	410.5
90	290.5
91	210.0
92	136.5
93	102.0
94	80.5
95	67.0
96	62.5
97	61.5
98	51.5
99	42.5
100	42.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.038599627486130605
2	0.011060944305024389
3	0.0035454945627843423
4	0.003133519646668819
5	0.0012733851884079502
6	0.002783973401693405
7	2.2471652010988137E-4
8	0.0015730160335277132
9	8.738984510025113E-5
10-14	4.394464293329692E-4
15-19	0.011835144526590223
20-24	0.011848265197984513
25-29	0.00884283279411865
30-34	0.010002114304611806
35-39	0.006593387491684589
40-44	0.010529754811811978
45-49	0.010085483086022952
50-54	0.010609974171469243
55-59	0.010084931710569265
60-64	0.03819539952076662
65-69	0.02964756333783603
70-74	0.018206276674139057
75-79	0.038829869859117475
80-84	0.045288773599805286
85-89	0.012571494293143737
90-94	0.002506579110539083
95-99	0.00689718459650508
100	0.013036507093321898
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
0-1	274.0
2-3	4.0
4-5	28.0
6-7	44.0
8-9	6.0
10-11	8.0
12-13	0.0
14-15	10.0
16-17	32.0
18-19	86.0
20-21	388.0
22-23	209.0
24-25	517.0
26-27	203.0
28-29	137.0
30-31	252.0
32-33	85.0
34-35	90.0
36-37	78.0
38-39	87.0
40-41	138.0
42-43	90.0
44-45	43.0
46-47	129.0
48-49	163.0
50-51	20074.0
52-53	581.0
54-55	24579.0
56-57	39762.0
58-59	73621.0
60-61	1343.0
62-63	39689.0
64-65	19706.0
66-67	13452.0
68-69	21321.0
70-71	32859.0
72-73	38450.0
74-75	15459.0
76-77	11795.0
78-79	4589.0
80-81	10877.0
82-83	17634.0
84-85	11302.0
86-87	5892.0
88-89	8820.0
90-91	24351.0
92-93	2203.0
94-95	9181.0
96-97	172851.0
98-99	0.0
100-101	7386948.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	98.49406411687556
#Duplication Level	Relative count
1	100.0
2	13.522116851250082
3	4.830393262152663
4	2.311990185025532
5	1.251740831620134
6	0.683898136481199
7	0.4476424166058757
8	0.3249552357583394
9	0.24288745938059553
10++	7.805557397705418
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CATGCACTATCCAGGCGCCTTCACCTACTCCCCGACGCCGGTCACCTCGGGCATCGGCATCGG	15231	0.19013936812459742	No Hit
CCGATGCCGATGCCCGAGGTGACCGGCGTCGGGGAGTAGGTGAAGGCGCCTGGATAGTGCATG	14637	0.18272404512111695	No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG	13287	0.165871038295025	No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA	13121	0.16379874264085367	No Hit
GACAACTTGGCCACCGACAGCTGAAGGGCTTTTCACCTGTTGACACAATTGCCAGTCA	12946	0.16161409360784176	No Hit
TGACTGGCAATTGTGTCAACAGGTGAAAAGCCCTTCAGCTGTCGGTGGCCAAGTTGTC	12887	0.16087755479099775	No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT	11976	0.14950489611057569	No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT	11873	0.1482190741082887	No Hit
GAGCTACCATCTGATCTGTCTGTCTTGACCACCCGGAGTCCCACTGTCCCCAGCCAGAATCCCAGTAGACTA	10759	0.13431222254957279	No Hit
TAGTCTACTGGGATTCTGGCTGGGGACAGTGGGACTCCGGGTGGTCAAGACAGACAGATCAGATGGTAGCTC	10529	0.13144096953475715	No Hit
ATTCAGCTGCCAAGTAACTTAGCACACCCATTCTTATAAGATAATGGCAA	10462	0.13060456104783258	No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC	10420	0.13008024527990972	No Hit
TAATGAGCATGAAGTCACCACACGGAGGAAAATGTAAATGTGTAAACCTC	10399	0.12981808739594827	No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG	10382	0.12960586434702714	No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG	10174	0.12700925292493298	No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA	10164	0.1268844158373323	No Hit
CATGTAATTCAGGCAGTTATGGGAGCCCTAGAGGGCCTGAGAGTTGCTATTGGAC	10094	0.12601055622412752	No Hit
GTCCAATAGCAACTCTCAGGCCCTCTAGGGCTCCCATAACTGCCTGAATTACATG	10069	0.1256984635051258	No Hit
CATGGAATATATATTCTCCTGGCTGGTGCTTATGATTGCAAAACTTGGAT	9531	0.11898222819220916	No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA	9516	0.11879497256080815	No Hit
CACACTGTCATTAGTTGCAGCATCTCCTGTAGCAATTGGGCTAAATCTGT	9484	0.11839549388048597	No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC	9475	0.11828314050164536	No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT	9115	0.11378900534802082	No Hit
GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG	9076	0.11330214070637817	No Hit
GAAGGGCAGGAGTTCCTTTGGTGACTTCACAGTGAAGTCTTGCCCTCTCT	9014	0.11252815076325397	No Hit
TAGCTGCTCCATTTTCATTTCACCACTTTGAAATCTTGGAACCACTGATG	8991	0.11224102546177239	No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA	8931	0.11149200293616829	No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC	8911	0.11124232876096693	No Hit
ATCCTTCGAGGGCTTTGTCTGACAACTGTCTTATCTTCTTTTGTAAGTGG	8816	0.11005637642876047	No Hit
TAATTCTGAACTGGAAAAGCCCCAGAAAGTCCGGAAAGACAAGGAAGGAA	8808	0.10995650675867993	No Hit
CATGCTTTCCCTGGGTCTTCACGGATTGCTTTCCAAGCTGCCTTGTTGCG	8707	0.10869565217391304	No Hit
GCGACAGCTGGAAAGGTCCAGTTGCTGCTCTGCAGCAACCCCGCAACAAG	8678	0.10833362461987107	No Hit
ACAGTGAAAACAGTGACAATGTTCCAGCAAAGCCACCAGACAAAGTTCTT	8662	0.10813388527970996	No Hit
CATGGCTCTGCTGTGCCTTCCATCCTGGGCTCCCTTCTCTCCTGTGACCT	8660	0.10810891786218985	No Hit
CATGTCATCTCAAAGGTCCCCAGGATTCGAACACCCAGTTATTCTCCAAC	8577	0.1070727700351042	No Hit
ACCTATGTTACACCATCTTCAGTGCCAGTCTTGGGCAAAATTGTGCAAGA	8558	0.1068355795686629	No Hit
AGCCACCTCACCCACCCGGAATCTACATTTTCAATCAGTAAAAGTCACCA	8520	0.10636119863578031	No Hit
AGTTGACAACTTGGACTTGGCCAATACTGCATTCTAAATCACCACATCAT	8467	0.10569956207149668	No Hit
TAAAACTGCATCAAGTCATGGGGCATGTGGAAGGTAGGGAGGCAAGATGA	8458	0.10558720869265609	No Hit
TAATCTGATGTGGCATTTTCGTCATCTGAAGCATGAGTGACAAGTTGGGA	8436	0.1053125670999346	No Hit
CATGGGGTGGGTGCTTGCAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTT	8168	0.10196693315223633	No Hit
GGCTGCTTGTTGTACGTGGCCTGGTGGAACGCACTGCAAAACGAGCTCAG	8048	0.10046888810102815	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	3165910	3.075488	4.6996017	75-79
CCCAG	1683455	2.8425536	5.3444176	2
CCCCA	1477535	2.4575648	6.0637283	1
GGAGG	1372695	2.4249408	5.200161	4
CCTCC	1385260	2.3249862	5.5635214	6
TGGGG	1297970	2.3137348	8.418024	3
CTGCC	1351790	2.303235	5.7015214	7
GGGGG	1085425	2.2519414	5.95693	4
AGGAG	1465070	2.203714	5.0419726	6
GGAAG	1388015	2.08781	5.0031996	4
CCACC	1247170	2.0744016	6.1592574	1
TGGAG	1341835	2.0366566	5.1579456	3
TGGAA	1568975	2.0277038	5.198208	3
TGCAG	1353355	2.0234408	6.176947	3
GAGGG	1119935	1.9784263	5.2530713	8
TGGCT	1310365	1.9769373	6.6589236	3
CCCTG	1138860	1.9404361	5.4511685	1
GCCCA	1063785	1.7962261	6.46638	2
GGCTC	1037350	1.794297	8.0923605	1
GCTCC	940045	1.6016871	7.9522405	2
TGCCC	916445	1.5614765	9.498929	3
GATGG	1021395	1.5502882	5.141823	1
TAAAA	1641790	1.5383162	6.1052394	1
CATGG	994810	1.4873697	37.886646	1
TGAGG	966450	1.4668919	5.420493	1
ATGGA	1133395	1.4647713	10.974425	2
GGCCC	731950	1.4515059	6.844899	1
TCAGG	921870	1.378315	5.155067	1
GCTCT	917255	1.3631723	5.024858	2
TGGGT	870805	1.3337102	5.8511834	3
TGCTC	888505	1.3204457	5.685886	1
ATGTG	998685	1.3023834	9.981001	2
ATGGG	854045	1.2962819	11.653119	2
ATGGC	860495	1.2865514	11.326274	2
TGAGA	947035	1.2239243	5.3418326	1
ATGAA	1084955	1.1939056	8.678462	2
ATGCC	778970	1.1472538	8.603732	2
TGAGC	763625	1.1417181	5.7610903	1
ATGTT	981665	1.0999305	6.476009	2
ATGCT	837680	1.0760897	8.261295	2
CATGA	843240	1.0734943	24.179398	1
CATGT	830490	1.0668535	28.827944	1
ATGAG	805460	1.0409563	5.272833	2
ATGGT	760410	0.9916494	6.6603417	2
CATGC	656185	0.96641827	29.451681	1
ATGAC	743570	0.9466085	6.2376223	2
ATGTA	774525	0.86003387	7.178604	2
ATGCA	658965	0.83890134	10.672013	2
TAATG	732800	0.81370234	5.1035295	1
ATGTC	603760	0.7755945	5.039233	2
TAAGT	672800	0.74707824	6.789895	1
>>END_MODULE