FastQCFastQC Report
Wed 20 Mar 2013
8016-7-3.h.sapiens.aligned.bam

Summary

[OK] Basic Statistics

Measure Value
Filename 8016-7-3.h.sapiens.aligned.bam
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 8010440
Filtered Sequences 0
Sequence length 1-100
%GC 46

[WARN] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
CATGCACTATCCAGGCGCCTTCACCTACTCCCCGACGCCGGTCACCTCGGGCATCGGCATCGG 15231 0.19013936812459742 No Hit
CCGATGCCGATGCCCGAGGTGACCGGCGTCGGGGAGTAGGTGAAGGCGCCTGGATAGTGCATG 14637 0.18272404512111695 No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG 13287 0.165871038295025 No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA 13121 0.16379874264085367 No Hit
GACAACTTGGCCACCGACAGCTGAAGGGCTTTTCACCTGTTGACACAATTGCCAGTCA 12946 0.16161409360784176 No Hit
TGACTGGCAATTGTGTCAACAGGTGAAAAGCCCTTCAGCTGTCGGTGGCCAAGTTGTC 12887 0.16087755479099775 No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT 11976 0.14950489611057569 No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT 11873 0.1482190741082887 No Hit
GAGCTACCATCTGATCTGTCTGTCTTGACCACCCGGAGTCCCACTGTCCCCAGCCAGAATCCCAGTAGACTA 10759 0.13431222254957279 No Hit
TAGTCTACTGGGATTCTGGCTGGGGACAGTGGGACTCCGGGTGGTCAAGACAGACAGATCAGATGGTAGCTC 10529 0.13144096953475715 No Hit
ATTCAGCTGCCAAGTAACTTAGCACACCCATTCTTATAAGATAATGGCAA 10462 0.13060456104783258 No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC 10420 0.13008024527990972 No Hit
TAATGAGCATGAAGTCACCACACGGAGGAAAATGTAAATGTGTAAACCTC 10399 0.12981808739594827 No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG 10382 0.12960586434702714 No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG 10174 0.12700925292493298 No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA 10164 0.1268844158373323 No Hit
CATGTAATTCAGGCAGTTATGGGAGCCCTAGAGGGCCTGAGAGTTGCTATTGGAC 10094 0.12601055622412752 No Hit
GTCCAATAGCAACTCTCAGGCCCTCTAGGGCTCCCATAACTGCCTGAATTACATG 10069 0.1256984635051258 No Hit
CATGGAATATATATTCTCCTGGCTGGTGCTTATGATTGCAAAACTTGGAT 9531 0.11898222819220916 No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA 9516 0.11879497256080815 No Hit
CACACTGTCATTAGTTGCAGCATCTCCTGTAGCAATTGGGCTAAATCTGT 9484 0.11839549388048597 No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC 9475 0.11828314050164536 No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT 9115 0.11378900534802082 No Hit
GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG 9076 0.11330214070637817 No Hit
GAAGGGCAGGAGTTCCTTTGGTGACTTCACAGTGAAGTCTTGCCCTCTCT 9014 0.11252815076325397 No Hit
TAGCTGCTCCATTTTCATTTCACCACTTTGAAATCTTGGAACCACTGATG 8991 0.11224102546177239 No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA 8931 0.11149200293616829 No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC 8911 0.11124232876096693 No Hit
ATCCTTCGAGGGCTTTGTCTGACAACTGTCTTATCTTCTTTTGTAAGTGG 8816 0.11005637642876047 No Hit
TAATTCTGAACTGGAAAAGCCCCAGAAAGTCCGGAAAGACAAGGAAGGAA 8808 0.10995650675867993 No Hit
CATGCTTTCCCTGGGTCTTCACGGATTGCTTTCCAAGCTGCCTTGTTGCG 8707 0.10869565217391304 No Hit
GCGACAGCTGGAAAGGTCCAGTTGCTGCTCTGCAGCAACCCCGCAACAAG 8678 0.10833362461987107 No Hit
ACAGTGAAAACAGTGACAATGTTCCAGCAAAGCCACCAGACAAAGTTCTT 8662 0.10813388527970996 No Hit
CATGGCTCTGCTGTGCCTTCCATCCTGGGCTCCCTTCTCTCCTGTGACCT 8660 0.10810891786218985 No Hit
CATGTCATCTCAAAGGTCCCCAGGATTCGAACACCCAGTTATTCTCCAAC 8577 0.1070727700351042 No Hit
ACCTATGTTACACCATCTTCAGTGCCAGTCTTGGGCAAAATTGTGCAAGA 8558 0.1068355795686629 No Hit
AGCCACCTCACCCACCCGGAATCTACATTTTCAATCAGTAAAAGTCACCA 8520 0.10636119863578031 No Hit
AGTTGACAACTTGGACTTGGCCAATACTGCATTCTAAATCACCACATCAT 8467 0.10569956207149668 No Hit
TAAAACTGCATCAAGTCATGGGGCATGTGGAAGGTAGGGAGGCAAGATGA 8458 0.10558720869265609 No Hit
TAATCTGATGTGGCATTTTCGTCATCTGAAGCATGAGTGACAAGTTGGGA 8436 0.1053125670999346 No Hit
CATGGGGTGGGTGCTTGCAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTT 8168 0.10196693315223633 No Hit
GGCTGCTTGTTGTACGTGGCCTGGTGGAACGCACTGCAAAACGAGCTCAG 8048 0.10046888810102815 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 3165910 3.075488 4.6996017 75-79
CCCAG 1683455 2.8425536 5.3444176 2
CCCCA 1477535 2.4575648 6.0637283 1
GGAGG 1372695 2.4249408 5.200161 4
CCTCC 1385260 2.3249862 5.5635214 6
TGGGG 1297970 2.3137348 8.418024 3
CTGCC 1351790 2.303235 5.7015214 7
GGGGG 1085425 2.2519414 5.95693 4
AGGAG 1465070 2.203714 5.0419726 6
GGAAG 1388015 2.08781 5.0031996 4
CCACC 1247170 2.0744016 6.1592574 1
TGGAG 1341835 2.0366566 5.1579456 3
TGGAA 1568975 2.0277038 5.198208 3
TGCAG 1353355 2.0234408 6.176947 3
GAGGG 1119935 1.9784263 5.2530713 8
TGGCT 1310365 1.9769373 6.6589236 3
CCCTG 1138860 1.9404361 5.4511685 1
GCCCA 1063785 1.7962261 6.46638 2
GGCTC 1037350 1.794297 8.0923605 1
GCTCC 940045 1.6016871 7.9522405 2
TGCCC 916445 1.5614765 9.498929 3
GATGG 1021395 1.5502882 5.141823 1
TAAAA 1641790 1.5383162 6.1052394 1
CATGG 994810 1.4873697 37.886646 1
TGAGG 966450 1.4668919 5.420493 1
ATGGA 1133395 1.4647713 10.974425 2
GGCCC 731950 1.4515059 6.844899 1
TCAGG 921870 1.378315 5.155067 1
GCTCT 917255 1.3631723 5.024858 2
TGGGT 870805 1.3337102 5.8511834 3
TGCTC 888505 1.3204457 5.685886 1
ATGTG 998685 1.3023834 9.981001 2
ATGGG 854045 1.2962819 11.653119 2
ATGGC 860495 1.2865514 11.326274 2
TGAGA 947035 1.2239243 5.3418326 1
ATGAA 1084955 1.1939056 8.678462 2
ATGCC 778970 1.1472538 8.603732 2
TGAGC 763625 1.1417181 5.7610903 1
ATGTT 981665 1.0999305 6.476009 2
ATGCT 837680 1.0760897 8.261295 2
CATGA 843240 1.0734943 24.179398 1
CATGT 830490 1.0668535 28.827944 1
ATGAG 805460 1.0409563 5.272833 2
ATGGT 760410 0.9916494 6.6603417 2
CATGC 656185 0.96641827 29.451681 1
ATGAC 743570 0.9466085 6.2376223 2
ATGTA 774525 0.86003387 7.178604 2
ATGCA 658965 0.83890134 10.672013 2
TAATG 732800 0.81370234 5.1035295 1
ATGTC 603760 0.7755945 5.039233 2
TAAGT 672800 0.74707824 6.789895 1
Produced by FastQC (version 0.10.0)