##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	8016-7-37.h.sapiens.aligned.bam	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	8598466	
Filtered Sequences	0	
Sequence length	1-100	
%GC	45	
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.834217870955122	24.0	24.0	32.0	24.0	32.0
2	31.01522856099804	31.0	31.0	32.0	30.0	32.0
3	32.69998533445184	34.0	33.0	34.0	30.0	34.0
4	34.2725759017713	35.0	35.0	35.0	29.0	37.0
5	35.39262821687013	36.0	36.0	36.0	34.0	36.0
6	35.58062527381621	36.0	36.0	37.0	34.0	37.0
7	35.93071339900013	37.0	35.0	37.0	33.0	38.0
8	36.27820291713871	37.0	36.0	37.0	34.0	38.0
9	36.51581727705696	37.0	37.0	38.0	35.0	38.0
10-14	36.78717081763571	37.6	36.8	38.0	34.8	38.2
15-19	37.28556360731007	38.0	37.0	38.8	34.8	39.2
20-24	36.9123742672057	37.8	36.8	38.2	35.6	38.6
25-29	37.04583415959637	38.0	36.8	38.6	34.2	39.0
30-34	37.03938017284731	37.8	36.8	38.8	35.4	39.2
35-39	36.95955688790292	38.0	37.2	38.6	33.8	39.2
40-44	36.828457043400974	38.0	36.8	38.2	33.6	39.2
45-49	36.69158337884241	37.4	37.0	38.4	33.8	39.2
50-54	36.92859362406987	38.2	37.2	38.2	34.0	39.0
55-59	36.994166829379274	38.0	37.2	38.4	34.0	38.8
60-64	36.46810860684458	37.4	36.4	38.0	33.4	38.4
65-69	36.56831195133297	37.8	36.4	38.4	34.0	38.8
70-74	36.61134614093651	37.6	36.8	38.6	34.2	38.6
75-79	36.272958286433166	37.2	36.8	37.8	34.4	38.6
80-84	36.52355071860196	37.8	36.4	38.0	34.0	38.8
85-89	36.46075385608851	38.0	36.2	38.6	33.0	39.2
90-94	36.27060160482584	37.6	36.4	38.4	33.2	39.6
95-99	35.962586241570015	37.2	36.4	38.0	32.6	38.6
100	34.97532092243713	37.0	35.0	37.0	32.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	0.0
5	45.0
6	190.0
7	344.0
8	415.0
9	719.0
10	865.0
11	1118.0
12	1354.0
13	1651.0
14	1990.0
15	2372.0
16	2860.0
17	3626.0
18	4664.0
19	4945.0
20	5152.0
21	6131.0
22	7606.0
23	9446.0
24	12432.0
25	16428.0
26	22857.0
27	31169.0
28	42464.0
29	56421.0
30	74848.0
31	99368.0
32	133591.0
33	182462.0
34	272482.0
35	521970.0
36	1752619.0
37	5320940.0
38	2919.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.478362965135602	17.408102592395366	21.068780925320134	39.0447535171489
2	20.11708068285046	46.649801279210564	17.11740286990738	16.1157151680316
3	19.28562514661637	34.4615839813336	30.045606486721105	16.20718438532893
4	34.07221053493455	23.945259763278926	20.683785415815706	21.29874428597082
5	21.881390450344774	26.782464258254475	25.875050111772367	25.46109517962839
6	21.818801189743198	28.183489998671806	25.715973118893604	24.281735692691388
7	22.476824041821978	27.401257217954605	26.5772619165305	23.544656823692918
8	22.38024415217805	26.567738715021346	27.709739337474026	23.34227779532658
9	21.939505123542336	28.488449176516234	26.09094212050966	23.48110357943177
10-14	22.561495933316372	26.980425591619863	26.66110929447471	23.796969180589056
15-19	21.863310495932975	27.353308079344142	27.231806328968926	23.55157509575396
20-24	22.14641623378178	26.258625505769075	27.655638195823677	23.93932006462547
25-29	22.228278678846635	26.878458594693967	28.000308031657926	22.892954694801464
30-34	22.24462451901681	27.48373037654658	27.368625256836136	22.90301984760048
35-39	22.5826990288538	26.631377020026047	28.249247511700908	22.536676439419246
40-44	22.450583490042636	27.19683657273943	27.510800418174824	22.841779519043108
45-49	22.628630516160136	27.85942877831491	27.47582215380377	22.036118551721184
50-54	22.582907197217015	27.436335152293452	27.354627453935887	22.62613019655365
55-59	22.55876234378864	27.72344995016926	27.40031686772102	22.317470838321082
60-64	22.487912553665396	27.485360576306256	27.998556796448586	22.028170073579762
65-69	22.554824919392637	27.951005528251265	27.868661397275062	21.625508155081032
70-74	22.371811171181	28.056451635947667	27.320685863071116	22.251051329800216
75-79	22.875122375607422	27.87604797712071	27.289126805180164	21.959702842091705
80-84	22.019476784497265	28.10640794591978	27.33824194634842	22.535873323234533
85-89	22.365650668397787	28.13356723235604	27.48634411848707	22.0144379807591
90-94	22.38695919975115	27.2666379834903	27.910631207433216	22.43577160932533
95-99	22.337878656555844	27.132466911775765	27.873578210605498	22.656076221062897
100	23.512059144162762	26.245045702972412	26.90734604010544	23.335549112759384
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	61.5231164822845
2	36.23279585088206
3	35.492809531945305
4	55.37095482090537
5	47.34248562997316
6	46.10053688243458
7	46.021480865514896
8	45.72252194750463
9	45.42060870297411
10-14	46.35846511390543
15-19	45.41488559168693
20-24	46.08573629840725
25-29	45.1212333736481
30-34	45.147644366617286
35-39	45.11937546827304
40-44	45.29236300908575
45-49	44.664749067881324
50-54	45.209037393770664
55-59	44.87623318210972
60-64	44.516082627245154
65-69	44.18033307447367
70-74	44.62286250098122
75-79	44.83482521769913
80-84	44.5553501077318
85-89	44.38008864915689
90-94	44.822730809076475
95-99	44.99395487761874
100	46.84760825692214
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	92.0
1	92.0
2	95.0
3	100.5
4	100.5
5	107.0
6	127.5
7	142.5
8	170.0
9	204.0
10	234.5
11	270.5
12	319.5
13	425.5
14	501.0
15	799.5
16	985.0
17	1006.0
18	1638.0
19	2292.5
20	4282.0
21	6085.0
22	9810.0
23	16976.0
24	28478.5
25	46242.0
26	62219.5
27	75441.5
28	90212.5
29	126340.0
30	170671.0
31	177424.0
32	179618.5
33	207353.0
34	254789.0
35	268376.5
36	248349.5
37	238300.5
38	260658.0
39	292160.5
40	315563.0
41	316785.5
42	290115.5
43	312415.5
44	344412.0
45	337384.0
46	299424.0
47	257424.5
48	263470.0
49	279755.0
50	283687.5
51	293942.0
52	250681.0
53	193863.0
54	177289.5
55	154163.0
56	128039.5
57	112213.0
58	135535.0
59	142996.0
60	131446.5
61	144367.5
62	129491.5
63	106394.5
64	83648.5
65	76658.5
66	100172.5
67	94489.5
68	59557.5
69	43038.0
70	34331.0
71	29515.5
72	27765.5
73	16667.5
74	9943.5
75	11896.5
76	12548.0
77	11733.0
78	8426.0
79	7256.5
80	6903.0
81	6798.0
82	4766.0
83	2670.0
84	2580.5
85	1431.0
86	3286.0
87	2679.0
88	513.0
89	364.0
90	242.5
91	180.5
92	135.0
93	93.5
94	60.5
95	48.5
96	40.0
97	32.0
98	27.0
99	20.0
100	17.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03830915886624428
2	0.010385673678411395
3	0.0030703447380745893
4	0.0028726331450925134
5	0.0011862698338210413
6	0.0025469922986628758
7	9.304102015756962E-5
8	0.0013142045625693375
9	3.489041096368362E-5
10-14	3.6053473302065744E-4
15-19	0.011758201962272943
20-24	0.011633129477917432
25-29	0.008030512784764788
30-34	0.009442879200170683
35-39	0.005979019411503033
40-44	0.01040419337384563
45-49	0.009611113769878294
50-54	0.009666876993909961
55-59	0.009509773409763162
60-64	0.03739177383346283
65-69	0.028934175918025305
70-74	0.017643815704546924
75-79	0.038834273889218895
80-84	0.04410653665145975
85-89	0.012255076552441452
90-94	0.0025087340361565225
95-99	0.0062757657465673305
100	0.011434837625936079
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
0-1	83.0
2-3	0.0
4-5	6.0
6-7	20.0
8-9	8.0
10-11	12.0
12-13	4.0
14-15	70.0
16-17	16.0
18-19	37.0
20-21	417.0
22-23	107.0
24-25	431.0
26-27	97.0
28-29	101.0
30-31	108.0
32-33	95.0
34-35	96.0
36-37	63.0
38-39	72.0
40-41	93.0
42-43	78.0
44-45	106.0
46-47	112.0
48-49	89.0
50-51	24211.0
52-53	659.0
54-55	23580.0
56-57	32202.0
58-59	70279.0
60-61	2221.0
62-63	33967.0
64-65	13525.0
66-67	14294.0
68-69	15312.0
70-71	36997.0
72-73	34158.0
74-75	22024.0
76-77	19042.0
78-79	9857.0
80-81	18704.0
82-83	24245.0
84-85	11901.0
86-87	7668.0
88-89	7845.0
90-91	26198.0
92-93	6270.0
94-95	8066.0
96-97	201019.0
98-99	0.0
100-101	7931901.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	98.42055548047756
#Duplication Level	Relative count
1	100.0
2	12.946218190386428
3	4.310497172478794
4	1.8504064561734213
5	0.9785874175306315
6	0.6148385956644675
7	0.36153982092365694
8	0.28201578699340246
9	0.20617342130065974
10++	7.120346371347785
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CATGCACTATCCAGGCGCCTTCACCTACTCCCCGACGCCGGTCACCTCGGGCATCGGCATCGG	11464	0.13332610723819807	No Hit
CCGATGCCGATGCCCGAGGTGACCGGCGTCGGGGAGTAGGTGAAGGCGCCTGGATAGTGCATG	11098	0.1290695340308376	No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC	10866	0.12637137833655446	No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG	10855	0.12624344854070482	No Hit
CATGCTTTCCCTGGGTCTTCACGGATTGCTTTCCAAGCTGCCTTGTTGCG	10818	0.12581313922739243	No Hit
GCGACAGCTGGAAAGGTCCAGTTGCTGCTCTGCAGCAACCCCGCAACAAG	10773	0.125289790062553	No Hit
ATCCTTCACAAAGTCTTTGAGATATATTTTTATCAAATATTTAGCATGGATCCCGGTACACTTTCAATACTTA	9995	0.1162416645015518	No Hit
TAAGTATTGAAAGTGTACCGGGATCCATGCTAAATATTTGATAAAAATATATCTCAAAGACTTTGTGAAGGAT	9940	0.11560201552230362	No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA	9914	0.11529963600484087	No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC	9882	0.11492747659873286	No Hit
GACAACTTGGCCACCGACAGCTGAAGGGCTTTTCACCTGTTGACACAATTGCCAGTCA	9816	0.11415989782363506	No Hit
TGACTGGCAATTGTGTCAACAGGTGAAAAGCCCTTCAGCTGTCGGTGGCCAAGTTGTC	9778	0.11371795852888178	No Hit
CATGTAGACTTACCTTTCCTCATAGAGCTATCCTGGTTAATAACAGGCCA	9412	0.1094613853215213	No Hit
ACACTTACACCAAGGAATCTCCCAGGCATTATCAAAGCTACAGGAGACAA	9375	0.1090310760082089	No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA	8664	0.10076215920374633	No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG	8650	0.10059933946357409	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	3838560	3.1404827	4.340371	95-96
AAAAA	3954825	3.0750792	5.383879	2
CCCAG	1708675	2.907213	5.1420274	2
GGAGG	1497740	2.6353402	5.049282	4
GGGGG	1189870	2.5678375	5.8929605	4
CCCCA	1515240	2.5493994	6.2802076	1
CCTCC	1441600	2.45031	5.732795	6
TGGGG	1342910	2.3870802	8.6180725	3
CCACC	1267665	2.132853	5.7519655	1
TGGCT	1320445	1.9117515	6.180281	3
TGCAG	1284265	1.8405429	6.387155	3
GCCCA	1073545	1.8265754	5.808334	2
GGCTC	1038045	1.8043234	6.579027	1
GCTCC	947135	1.62798	6.224154	2
TGCCC	927905	1.5949267	8.820272	3
TAAAA	2017105	1.5844455	7.112305	1
GGCCC	728300	1.5198319	6.0382924	1
CATGG	1041575	1.492732	36.641476	1
ATGGA	1217930	1.4391525	10.034484	2
TCAGG	998995	1.4317086	5.358905	1
TGGGT	895535	1.3111569	5.5495915	3
ATGTG	1094020	1.305959	9.508759	2
ATGGG	881535	1.277591	11.612711	2
ATGGC	881185	1.2628692	10.206316	2
ATGAA	1244490	1.1989692	7.5789113	2
TGAGA	1012760	1.1967158	5.684038	1
ATGCC	802100	1.1367341	6.822869	2
TGAGC	788875	1.1305753	5.082582	1
CATGT	917950	1.0835835	28.659557	1
ATGTT	1095070	1.0767086	7.0323095	2
ATGCT	877520	1.0358583	8.192999	2
CATGA	885340	1.0345073	22.711136	1
ATGGT	833960	0.99551886	6.382676	2
ATGAC	820400	0.95862585	6.0940943	2
CATGC	656840	0.93087196	27.800396	1
ATGTA	937595	0.91253966	6.860658	2
ATGCA	729480	0.8523871	9.905174	2
TAAGT	775475	0.7547519	7.1189876	1
TAAGG	573905	0.6781479	5.04177	1
>>END_MODULE