FastQCFastQC Report
Wed 20 Mar 2013
8016-7-37.h.sapiens.aligned.bam

Summary

[OK] Basic Statistics

Measure Value
Filename 8016-7-37.h.sapiens.aligned.bam
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 8598466
Filtered Sequences 0
Sequence length 1-100
%GC 45

[WARN] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
CATGCACTATCCAGGCGCCTTCACCTACTCCCCGACGCCGGTCACCTCGGGCATCGGCATCGG 11464 0.13332610723819807 No Hit
CCGATGCCGATGCCCGAGGTGACCGGCGTCGGGGAGTAGGTGAAGGCGCCTGGATAGTGCATG 11098 0.1290695340308376 No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC 10866 0.12637137833655446 No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG 10855 0.12624344854070482 No Hit
CATGCTTTCCCTGGGTCTTCACGGATTGCTTTCCAAGCTGCCTTGTTGCG 10818 0.12581313922739243 No Hit
GCGACAGCTGGAAAGGTCCAGTTGCTGCTCTGCAGCAACCCCGCAACAAG 10773 0.125289790062553 No Hit
ATCCTTCACAAAGTCTTTGAGATATATTTTTATCAAATATTTAGCATGGATCCCGGTACACTTTCAATACTTA 9995 0.1162416645015518 No Hit
TAAGTATTGAAAGTGTACCGGGATCCATGCTAAATATTTGATAAAAATATATCTCAAAGACTTTGTGAAGGAT 9940 0.11560201552230362 No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA 9914 0.11529963600484087 No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC 9882 0.11492747659873286 No Hit
GACAACTTGGCCACCGACAGCTGAAGGGCTTTTCACCTGTTGACACAATTGCCAGTCA 9816 0.11415989782363506 No Hit
TGACTGGCAATTGTGTCAACAGGTGAAAAGCCCTTCAGCTGTCGGTGGCCAAGTTGTC 9778 0.11371795852888178 No Hit
CATGTAGACTTACCTTTCCTCATAGAGCTATCCTGGTTAATAACAGGCCA 9412 0.1094613853215213 No Hit
ACACTTACACCAAGGAATCTCCCAGGCATTATCAAAGCTACAGGAGACAA 9375 0.1090310760082089 No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA 8664 0.10076215920374633 No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG 8650 0.10059933946357409 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 3838560 3.1404827 4.340371 95-96
AAAAA 3954825 3.0750792 5.383879 2
CCCAG 1708675 2.907213 5.1420274 2
GGAGG 1497740 2.6353402 5.049282 4
GGGGG 1189870 2.5678375 5.8929605 4
CCCCA 1515240 2.5493994 6.2802076 1
CCTCC 1441600 2.45031 5.732795 6
TGGGG 1342910 2.3870802 8.6180725 3
CCACC 1267665 2.132853 5.7519655 1
TGGCT 1320445 1.9117515 6.180281 3
TGCAG 1284265 1.8405429 6.387155 3
GCCCA 1073545 1.8265754 5.808334 2
GGCTC 1038045 1.8043234 6.579027 1
GCTCC 947135 1.62798 6.224154 2
TGCCC 927905 1.5949267 8.820272 3
TAAAA 2017105 1.5844455 7.112305 1
GGCCC 728300 1.5198319 6.0382924 1
CATGG 1041575 1.492732 36.641476 1
ATGGA 1217930 1.4391525 10.034484 2
TCAGG 998995 1.4317086 5.358905 1
TGGGT 895535 1.3111569 5.5495915 3
ATGTG 1094020 1.305959 9.508759 2
ATGGG 881535 1.277591 11.612711 2
ATGGC 881185 1.2628692 10.206316 2
ATGAA 1244490 1.1989692 7.5789113 2
TGAGA 1012760 1.1967158 5.684038 1
ATGCC 802100 1.1367341 6.822869 2
TGAGC 788875 1.1305753 5.082582 1
CATGT 917950 1.0835835 28.659557 1
ATGTT 1095070 1.0767086 7.0323095 2
ATGCT 877520 1.0358583 8.192999 2
CATGA 885340 1.0345073 22.711136 1
ATGGT 833960 0.99551886 6.382676 2
ATGAC 820400 0.95862585 6.0940943 2
CATGC 656840 0.93087196 27.800396 1
ATGTA 937595 0.91253966 6.860658 2
ATGCA 729480 0.8523871 9.905174 2
TAAGT 775475 0.7547519 7.1189876 1
TAAGG 573905 0.6781479 5.04177 1
Produced by FastQC (version 0.10.0)