##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	8016-7-4.h.sapiens.aligned.bam	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	8772498	
Filtered Sequences	0	
Sequence length	1-100	
%GC	46	
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.83866077826407	24.0	24.0	32.0	24.0	32.0
2	31.015288188038568	31.0	31.0	32.0	30.0	32.0
3	32.7139901093507	34.0	33.0	34.0	30.0	34.0
4	34.27864080746657	35.0	35.0	35.0	29.0	37.0
5	35.39779706626607	36.0	36.0	36.0	34.0	36.0
6	35.57812633231999	36.0	36.0	37.0	34.0	37.0
7	35.934870003793776	37.0	35.0	37.0	33.0	38.0
8	36.275211388280724	37.0	36.0	37.0	34.0	38.0
9	36.51327140467137	37.0	37.0	38.0	35.0	38.0
10-14	36.786485938054206	37.6	36.8	38.0	34.8	38.2
15-19	37.29018460016173	38.0	37.0	38.8	34.8	39.2
20-24	36.91381163373968	37.8	36.8	38.2	35.6	38.6
25-29	37.04175205543844	38.0	36.8	38.6	34.2	39.0
30-34	37.0331447067381	37.8	36.8	38.8	35.4	39.2
35-39	36.94516580128352	38.0	37.2	38.6	33.8	39.2
40-44	36.818630630135615	38.0	36.8	38.2	33.6	39.2
45-49	36.682423788786544	37.4	37.0	38.4	33.8	39.2
50-54	36.926999495287056	38.2	37.2	38.2	34.0	39.0
55-59	36.99067291772966	38.0	37.2	38.4	33.0	38.8
60-64	36.479010466862576	37.4	36.4	38.0	33.4	38.4
65-69	36.571490895564295	37.8	36.4	38.4	34.0	38.8
70-74	36.61929516760711	37.6	36.8	38.6	34.2	38.6
75-79	36.281157856261316	37.2	36.8	37.8	34.4	38.6
80-84	36.52765977350468	37.8	36.4	38.0	34.0	38.8
85-89	36.459414789899995	38.0	36.2	38.6	33.0	39.2
90-94	36.25521852812149	37.6	36.4	38.4	33.2	39.6
95-99	35.94942997113422	37.2	36.4	38.0	32.6	38.6
100	34.94309157290886	37.0	35.0	37.0	32.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	1.0
5	25.0
6	142.0
7	227.0
8	357.0
9	704.0
10	751.0
11	980.0
12	1316.0
13	1685.0
14	2068.0
15	2428.0
16	2970.0
17	3663.0
18	4933.0
19	5045.0
20	4903.0
21	5800.0
22	7149.0
23	9263.0
24	12216.0
25	16659.0
26	22804.0
27	31311.0
28	42662.0
29	57234.0
30	75851.0
31	100747.0
32	135382.0
33	187084.0
34	283121.0
35	548623.0
36	1835229.0
37	5366515.0
38	2649.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.290607014034684	16.149123217154997	21.100776714308033	39.45949305450229
2	19.71438975461401	47.08984641315268	16.967130617908996	16.228633214324315
3	21.7606836086856	31.64714554295035	29.476817012606883	17.115353835757173
4	34.8679799098822	23.1595059503602	20.091269179899758	21.881244959857842
5	22.7370562268879	25.930842135106268	24.709197508353387	26.62290412965244
6	22.65773269117297	27.624219453227383	24.763499001830358	24.95454885376929
7	23.05243938180919	26.12664742643358	26.089701345482563	24.731211846274665
8	22.991421588604922	25.631268640697247	27.16370024772755	24.213609522970287
9	23.136610489453094	27.760777178918953	25.644476211321166	23.458136120306786
10-14	23.522237497242994	25.979659472470836	25.649516566035903	24.84858646425027
15-19	22.648562179752986	26.0223945318971	26.54910257583975	24.77994071251017
20-24	22.94208916936539	25.2533105296754	26.419903158639702	25.384697142319514
25-29	22.851612469206962	26.149415438223215	27.43410702893705	23.564865063632773
30-34	22.80369166672539	26.990834506047406	26.720402729932864	23.485071097294345
35-39	22.988742111223424	26.33040908899447	27.61878715906162	23.062061640720486
40-44	22.762384788632893	27.06930480536039	26.941713849594112	23.22659655641261
45-49	23.008878460486017	27.22774485716959	27.214778789767113	22.548597892577284
50-54	23.088187442702115	27.05254665475384	27.125847282187838	22.733418620356215
55-59	22.789250110455	27.560476153837982	26.902604926042407	22.747668809664614
60-64	22.765119516289392	27.002035935622516	27.723615317614243	22.509229230473853
65-69	23.095181376217596	27.51538532710729	27.449341967340217	21.940091329334894
70-74	22.621137595490254	27.589610626371407	26.965250387854567	22.824001390283765
75-79	23.087183539196687	27.69523996860913	26.888741854766213	22.32883463742797
80-84	22.547544666827783	27.598140272217346	26.914685686567992	22.93962937438688
85-89	22.924938765499352	27.481690673118003	27.122294474621466	22.471076086761173
90-94	22.807739476561558	26.87928424978133	27.361975841653614	22.9510004320035
95-99	22.78688094227597	26.652488092963605	27.385665955428767	23.174965009331654
100	23.944135243479906	26.015040871290434	26.347222073439653	23.693601811790007
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	62.75010006853697
2	35.94302296893833
3	38.87603744444277
4	56.74922486974005
5	49.35996035654034
6	47.612281544942256
7	47.783651228083855
8	47.20503111157521
9	46.59474660975988
10-14	48.37082396149327
15-19	47.428502892263154
20-24	48.3267863116849
25-29	46.416477532839735
30-34	46.28876276401974
35-39	46.05080375194391
40-44	45.9889813450455
45-49	45.5574763530633
50-54	45.821606063058326
55-59	45.53691892011961
60-64	45.274348746763245
65-69	45.035272705552494
70-74	45.44513898577402
75-79	45.416018176624654
80-84	45.487174041214665
85-89	45.39601485226052
90-94	45.75873990856506
95-99	45.96184595160763
100	47.637737055269916
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	172.0
1	174.5
2	175.5
3	176.0
4	180.5
5	181.0
6	187.5
7	200.0
8	225.0
9	264.0
10	287.5
11	310.0
12	348.5
13	407.5
14	450.5
15	677.0
16	889.0
17	956.5
18	1262.0
19	1998.0
20	3407.0
21	4829.0
22	7860.0
23	13103.0
24	23639.5
25	36485.5
26	50408.0
27	62479.0
28	69493.0
29	90001.0
30	119510.0
31	136365.5
32	153104.5
33	172961.0
34	203992.5
35	219680.5
36	207434.5
37	208058.5
38	239705.5
39	283673.5
40	321401.0
41	324313.5
42	300890.5
43	334464.0
44	380851.5
45	373696.0
46	330911.0
47	278500.5
48	276848.5
49	311152.5
50	335631.5
51	355828.0
52	304528.0
53	222609.5
54	201834.0
55	185690.0
56	146679.0
57	118438.5
58	146539.5
59	157976.0
60	145516.5
61	151410.0
62	132652.5
63	108242.0
64	86254.0
65	85642.0
66	115233.5
67	109622.5
68	67325.0
69	48709.0
70	42789.5
71	34419.5
72	29371.0
73	19243.0
74	11522.5
75	13945.5
76	14336.5
77	13085.5
78	9319.0
79	7274.5
80	7083.5
81	6493.0
82	4292.5
83	2951.5
84	2804.0
85	1655.5
86	2948.0
87	2156.5
88	570.0
89	421.0
90	275.5
91	220.0
92	142.0
93	90.5
94	72.5
95	70.5
96	60.5
97	51.0
98	43.0
99	40.0
100	37.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03810773168600324
2	0.011445080484906629
3	0.0033058506945592312
4	0.002872670258720435
5	0.0012653463138865945
6	0.002690288923350933
7	1.481944895361011E-4
8	0.0012425552133398905
9	4.559836684889294E-5
10-14	3.9442624193936966E-4
15-19	0.01209727954617464
20-24	0.011856180066920385
25-29	0.008377627320496166
30-34	0.009752946486674902
35-39	0.006090965317436895
40-44	0.010275735895252623
45-49	0.009968167315608574
50-54	0.010117994919071461
55-59	0.009884440793452734
60-64	0.03821269881243863
65-69	0.029144745272209952
70-74	0.0183027762003026
75-79	0.03862946121393254
80-84	0.04488081322355774
85-89	0.012057231146716139
90-94	0.002302205166858981
95-99	0.006419202715577962
100	0.011868362135246387
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
0-1	170.0
2-3	2.0
4-5	34.0
6-7	46.0
8-9	3.0
10-11	8.0
12-13	2.0
14-15	0.0
16-17	12.0
18-19	60.0
20-21	475.0
22-23	134.0
24-25	500.0
26-27	158.0
28-29	75.0
30-31	177.0
32-33	58.0
34-35	179.0
36-37	83.0
38-39	40.0
40-41	119.0
42-43	94.0
44-45	98.0
46-47	114.0
48-49	110.0
50-51	20042.0
52-53	1030.0
54-55	22061.0
56-57	37997.0
58-59	80720.0
60-61	1224.0
62-63	38331.0
64-65	20977.0
66-67	14480.0
68-69	21360.0
70-71	31146.0
72-73	42019.0
74-75	15589.0
76-77	12790.0
78-79	5400.0
80-81	12481.0
82-83	18272.0
84-85	10791.0
86-87	6779.0
88-89	9225.0
90-91	24956.0
92-93	2297.0
94-95	9469.0
96-97	187876.0
98-99	0.0
100-101	8122435.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	98.4985804499471
#Duplication Level	Relative count
1	100.0
2	13.244047619047619
3	4.716350826044704
4	2.16912657920311
5	1.1342930029154519
6	0.6681243926141885
7	0.4411139455782313
8	0.31887755102040816
9	0.2346027696793003
10++	7.246112730806608
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CATGCACTATCCAGGCGCCTTCACCTACTCCCCGACGCCGGTCACCTCGGGCATCGGCATCGG	15540	0.17714452599476227	No Hit
CCGATGCCGATGCCCGAGGTGACCGGCGTCGGGGAGTAGGTGAAGGCGCCTGGATAGTGCATG	14984	0.1708065365190166	No Hit
GACAACTTGGCCACCGACAGCTGAAGGGCTTTTCACCTGTTGACACAATTGCCAGTCA	14416	0.1643317559035066	No Hit
TGACTGGCAATTGTGTCAACAGGTGAAAAGCCCTTCAGCTGTCGGTGGCCAAGTTGTC	14343	0.16349960980327383	No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG	13560	0.15457398793365357	No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA	13386	0.15259051640707127	No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG	11963	0.1363693670833553	No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC	11959	0.13632377003676718	No Hit
GAGCTACCATCTGATCTGTCTGTCTTGACCACCCGGAGTCCCACTGTCCCCAGCCAGAATCCCAGTAGACTA	11937	0.13607298628053263	No Hit
TAGTCTACTGGGATTCTGGCTGGGGACAGTGGGACTCCGGGTGGTCAAGACAGACAGATCAGATGGTAGCTC	11746	0.13389572730595095	No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT	11622	0.1324822188617199	No Hit
AGGACAACAGCTGATCCATACATTTGCTGACAGGTGTACCTGCGTTTCCC	11602	0.1322542336287794	No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT	11561	0.13178686390125138	No Hit
CTTGGGGGAGTGTGCCTCCAGCAGAGAAGTAATTCTGGCCTGGCAACCTA	11542	0.13157027792995793	No Hit
ATTCAGCTGCCAAGTAACTTAGCACACCCATTCTTATAAGATAATGGCAA	10852	0.12370478739351094	No Hit
TAATGAGCATGAAGTCACCACACGGAGGAAAATGTAAATGTGTAAACCTC	10806	0.12318042135774782	No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG	10649	0.12139073727916495	No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA	10643	0.1213223417092828	No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT	10228	0.1165916481257676	No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA	10183	0.11607868135165147	No Hit
GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG	10165	0.11587349464200504	No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC	10147	0.1156683079323586	No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA	10064	0.11472216921565558	No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC	10053	0.1145967773375383	No Hit
GAAGGGCAGGAGTTCCTTTGGTGACTTCACAGTGAAGTCTTGCCCTCTCT	9473	0.10798520558226402	No Hit
TAGCTGCTCCATTTTCATTTCACCACTTTGAAATCTTGGAACCACTGATG	9429	0.10748363806979495	No Hit
ACCTATGTTACACCATCTTCAGTGCCAGTCTTGGGCAAAATTGTGCAAGA	9369	0.10679968237097347	No Hit
CATGTCATCTCAAAGGTCCCCAGGATTCGAACACCCAGTTATTCTCCAAC	9317	0.10620692076532819	No Hit
AGCCACCTCACCCACCCGGAATCTACATTTTCAATCAGTAAAAGTCACCA	9315	0.10618412224203415	No Hit
ACAGTGAAAACAGTGACAATGTTCCAGCAAAGCCACCAGACAAAGTTCTT	9285	0.1058421443926234	No Hit
TAAAACTGCATCAAGTCATGGGGCATGTGGAAGGTAGGGAGGCAAGATGA	9252	0.10546596875827159	No Hit
CATGGCTCTGCTGTGCCTTCCATCCTGGGCTCCCTTCTCTCCTGTGACCT	9230	0.10521518500203705	No Hit
CATGGAATATATATTCTCCTGGCTGGTGCTTATGATTGCAAAACTTGGAT	9109	0.10383587434274708	No Hit
CACACTGTCATTAGTTGCAGCATCTCCTGTAGCAATTGGGCTAAATCTGT	9099	0.10372188172627682	No Hit
CATGTAATTCAGGCAGTTATGGGAGCCCTAGAGGGCCTGAGAGTTGCTATTGGAC	8893	0.10137363382698977	No Hit
GTCCAATAGCAACTCTCAGGCCCTCTAGGGCTCCCATAACTGCCTGAATTACATG	8867	0.10107725302416712	No Hit
CATGGAGGGGAGCACTCAGCACCTCCCTCACCCCACACCCTTGGCTGCTC	8834	0.1007010773898153	No Hit
GCCTCAGCCTTCCTTCGCAGAAAGTTGAGCAGGTCGCCATAGCAACAGTA	8798	0.10029070397052242	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	3582390	3.1723013	4.926294	75-79
CCCAG	1849475	2.8503218	5.2206054	2
CCCCA	1624990	2.4696834	6.0048957	1
GGAGG	1521920	2.445692	5.0939984	4
CCAGC	1570580	2.4205024	5.07837	5
CCTCC	1533560	2.350947	5.689213	6
TGGGG	1431090	2.3196816	8.826223	3
CTGCC	1470225	2.2854974	5.5968432	7
GGGGG	1182520	2.2295122	6.5592766	4
CTCCA	1595790	2.1143713	5.0538845	6
GGAAG	1529510	2.0949364	5.1290784	4
CCACC	1367915	2.0789773	6.1933103	1
TGGAA	1735255	2.043346	5.043254	3
TGGCT	1438480	1.976835	6.714501	3
TGCAG	1445465	1.9693493	5.9127607	3
CCCTG	1263545	1.9642085	5.3894787	1
GCCCA	1165440	1.7961202	5.5001907	2
GGCTC	1134200	1.7878925	7.352495	1
CAGCT	1257020	1.6888946	5.039894	8
GCTCC	1044995	1.6244677	7.1513357	2
TGCCC	1009445	1.5692046	9.301843	3
GATGG	1126950	1.5569501	5.0991635	1
TAAAA	1813295	1.5511874	6.3961835	1
CATGG	1095960	1.4931722	37.471485	1
TGAGG	1072255	1.4813856	5.002781	1
ATGGA	1255830	1.4788002	10.700529	2
ACAGC	1090895	1.4530878	5.4025745	7
GGCCC	787420	1.4237814	5.8938117	1
TGGGT	956325	1.3326833	5.8902082	3
ATGTG	1100205	1.3067837	10.0030985	2
ATGGG	936770	1.2942048	11.687946	2
ATGGC	934785	1.273582	11.171112	2
TGAGA	1041995	1.2269992	5.05611	1
GGAGT	880640	1.2166576	5.079563	7
ATGAA	1193715	1.1980857	8.510797	2
ATGCC	875815	1.176719	8.551817	2
TGAGC	837050	1.1404246	5.2366443	1
ATGTT	1070735	1.0933825	6.394727	2
CATGA	936265	1.087233	23.92281	1
ATGCT	906755	1.0620995	8.009023	2
CATGT	900355	1.054603	28.216122	1
ATGAG	882085	1.0386975	5.225143	2
ATGGT	832320	0.9885996	6.636644	2
CATGC	709410	0.95314217	28.324886	1
ATGAC	819550	0.9516982	6.428279	2
ATGTA	840215	0.8506072	6.75045	2
ATGCA	700080	0.81296426	10.114088	2
TAATG	801830	0.81174743	5.1886606	1
ATGTC	655675	0.7680046	5.1158175	2
TAAGT	727310	0.73630583	6.351611	1
>>END_MODULE