FastQCFastQC Report
Wed 20 Mar 2013
8016-7-4.h.sapiens.aligned.bam

Summary

[OK] Basic Statistics

Measure Value
Filename 8016-7-4.h.sapiens.aligned.bam
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 8772498
Filtered Sequences 0
Sequence length 1-100
%GC 46

[WARN] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
CATGCACTATCCAGGCGCCTTCACCTACTCCCCGACGCCGGTCACCTCGGGCATCGGCATCGG 15540 0.17714452599476227 No Hit
CCGATGCCGATGCCCGAGGTGACCGGCGTCGGGGAGTAGGTGAAGGCGCCTGGATAGTGCATG 14984 0.1708065365190166 No Hit
GACAACTTGGCCACCGACAGCTGAAGGGCTTTTCACCTGTTGACACAATTGCCAGTCA 14416 0.1643317559035066 No Hit
TGACTGGCAATTGTGTCAACAGGTGAAAAGCCCTTCAGCTGTCGGTGGCCAAGTTGTC 14343 0.16349960980327383 No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG 13560 0.15457398793365357 No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA 13386 0.15259051640707127 No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG 11963 0.1363693670833553 No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC 11959 0.13632377003676718 No Hit
GAGCTACCATCTGATCTGTCTGTCTTGACCACCCGGAGTCCCACTGTCCCCAGCCAGAATCCCAGTAGACTA 11937 0.13607298628053263 No Hit
TAGTCTACTGGGATTCTGGCTGGGGACAGTGGGACTCCGGGTGGTCAAGACAGACAGATCAGATGGTAGCTC 11746 0.13389572730595095 No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT 11622 0.1324822188617199 No Hit
AGGACAACAGCTGATCCATACATTTGCTGACAGGTGTACCTGCGTTTCCC 11602 0.1322542336287794 No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT 11561 0.13178686390125138 No Hit
CTTGGGGGAGTGTGCCTCCAGCAGAGAAGTAATTCTGGCCTGGCAACCTA 11542 0.13157027792995793 No Hit
ATTCAGCTGCCAAGTAACTTAGCACACCCATTCTTATAAGATAATGGCAA 10852 0.12370478739351094 No Hit
TAATGAGCATGAAGTCACCACACGGAGGAAAATGTAAATGTGTAAACCTC 10806 0.12318042135774782 No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG 10649 0.12139073727916495 No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA 10643 0.1213223417092828 No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT 10228 0.1165916481257676 No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA 10183 0.11607868135165147 No Hit
GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG 10165 0.11587349464200504 No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC 10147 0.1156683079323586 No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA 10064 0.11472216921565558 No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC 10053 0.1145967773375383 No Hit
GAAGGGCAGGAGTTCCTTTGGTGACTTCACAGTGAAGTCTTGCCCTCTCT 9473 0.10798520558226402 No Hit
TAGCTGCTCCATTTTCATTTCACCACTTTGAAATCTTGGAACCACTGATG 9429 0.10748363806979495 No Hit
ACCTATGTTACACCATCTTCAGTGCCAGTCTTGGGCAAAATTGTGCAAGA 9369 0.10679968237097347 No Hit
CATGTCATCTCAAAGGTCCCCAGGATTCGAACACCCAGTTATTCTCCAAC 9317 0.10620692076532819 No Hit
AGCCACCTCACCCACCCGGAATCTACATTTTCAATCAGTAAAAGTCACCA 9315 0.10618412224203415 No Hit
ACAGTGAAAACAGTGACAATGTTCCAGCAAAGCCACCAGACAAAGTTCTT 9285 0.1058421443926234 No Hit
TAAAACTGCATCAAGTCATGGGGCATGTGGAAGGTAGGGAGGCAAGATGA 9252 0.10546596875827159 No Hit
CATGGCTCTGCTGTGCCTTCCATCCTGGGCTCCCTTCTCTCCTGTGACCT 9230 0.10521518500203705 No Hit
CATGGAATATATATTCTCCTGGCTGGTGCTTATGATTGCAAAACTTGGAT 9109 0.10383587434274708 No Hit
CACACTGTCATTAGTTGCAGCATCTCCTGTAGCAATTGGGCTAAATCTGT 9099 0.10372188172627682 No Hit
CATGTAATTCAGGCAGTTATGGGAGCCCTAGAGGGCCTGAGAGTTGCTATTGGAC 8893 0.10137363382698977 No Hit
GTCCAATAGCAACTCTCAGGCCCTCTAGGGCTCCCATAACTGCCTGAATTACATG 8867 0.10107725302416712 No Hit
CATGGAGGGGAGCACTCAGCACCTCCCTCACCCCACACCCTTGGCTGCTC 8834 0.1007010773898153 No Hit
GCCTCAGCCTTCCTTCGCAGAAAGTTGAGCAGGTCGCCATAGCAACAGTA 8798 0.10029070397052242 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 3582390 3.1723013 4.926294 75-79
CCCAG 1849475 2.8503218 5.2206054 2
CCCCA 1624990 2.4696834 6.0048957 1
GGAGG 1521920 2.445692 5.0939984 4
CCAGC 1570580 2.4205024 5.07837 5
CCTCC 1533560 2.350947 5.689213 6
TGGGG 1431090 2.3196816 8.826223 3
CTGCC 1470225 2.2854974 5.5968432 7
GGGGG 1182520 2.2295122 6.5592766 4
CTCCA 1595790 2.1143713 5.0538845 6
GGAAG 1529510 2.0949364 5.1290784 4
CCACC 1367915 2.0789773 6.1933103 1
TGGAA 1735255 2.043346 5.043254 3
TGGCT 1438480 1.976835 6.714501 3
TGCAG 1445465 1.9693493 5.9127607 3
CCCTG 1263545 1.9642085 5.3894787 1
GCCCA 1165440 1.7961202 5.5001907 2
GGCTC 1134200 1.7878925 7.352495 1
CAGCT 1257020 1.6888946 5.039894 8
GCTCC 1044995 1.6244677 7.1513357 2
TGCCC 1009445 1.5692046 9.301843 3
GATGG 1126950 1.5569501 5.0991635 1
TAAAA 1813295 1.5511874 6.3961835 1
CATGG 1095960 1.4931722 37.471485 1
TGAGG 1072255 1.4813856 5.002781 1
ATGGA 1255830 1.4788002 10.700529 2
ACAGC 1090895 1.4530878 5.4025745 7
GGCCC 787420 1.4237814 5.8938117 1
TGGGT 956325 1.3326833 5.8902082 3
ATGTG 1100205 1.3067837 10.0030985 2
ATGGG 936770 1.2942048 11.687946 2
ATGGC 934785 1.273582 11.171112 2
TGAGA 1041995 1.2269992 5.05611 1
GGAGT 880640 1.2166576 5.079563 7
ATGAA 1193715 1.1980857 8.510797 2
ATGCC 875815 1.176719 8.551817 2
TGAGC 837050 1.1404246 5.2366443 1
ATGTT 1070735 1.0933825 6.394727 2
CATGA 936265 1.087233 23.92281 1
ATGCT 906755 1.0620995 8.009023 2
CATGT 900355 1.054603 28.216122 1
ATGAG 882085 1.0386975 5.225143 2
ATGGT 832320 0.9885996 6.636644 2
CATGC 709410 0.95314217 28.324886 1
ATGAC 819550 0.9516982 6.428279 2
ATGTA 840215 0.8506072 6.75045 2
ATGCA 700080 0.81296426 10.114088 2
TAATG 801830 0.81174743 5.1886606 1
ATGTC 655675 0.7680046 5.1158175 2
TAAGT 727310 0.73630583 6.351611 1
Produced by FastQC (version 0.10.0)