##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	8016-7-5.h.sapiens.aligned.bam	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	8627434	
Filtered Sequences	0	
Sequence length	1-100	
%GC	45	
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.84882098199766	24.0	24.0	32.0	24.0	32.0
2	31.01935028140532	31.0	31.0	32.0	30.0	32.0
3	32.71451587065527	34.0	33.0	34.0	30.0	34.0
4	34.27732864760429	35.0	35.0	35.0	29.0	37.0
5	35.401321973429546	36.0	36.0	36.0	34.0	36.0
6	35.58120221457529	36.0	36.0	37.0	34.0	37.0
7	35.935168385584	37.0	35.0	37.0	33.0	38.0
8	36.27771887821329	37.0	36.0	37.0	34.0	38.0
9	36.51843547779832	37.0	37.0	38.0	35.0	38.0
10-14	36.79065084016268	37.6	36.8	38.0	34.8	38.2
15-19	37.29342055130032	38.0	37.0	38.8	34.8	39.2
20-24	36.9200926683381	37.8	36.8	38.2	35.6	38.6
25-29	37.049442508105486	38.0	36.8	38.6	34.2	39.0
30-34	37.04978004573056	37.8	36.8	38.8	35.4	39.2
35-39	36.96551120592612	38.0	37.2	38.6	33.8	39.2
40-44	36.834787449877645	38.0	36.8	38.2	33.6	39.2
45-49	36.69554115613744	37.4	37.0	38.4	33.8	39.2
50-54	36.94526326888428	38.2	37.2	38.2	34.0	39.0
55-59	37.00199994338786	38.0	37.2	38.4	34.0	38.8
60-64	36.490314856134745	37.4	36.4	38.0	33.8	38.4
65-69	36.58834390236592	37.8	36.4	38.4	34.2	38.8
70-74	36.638506896669206	37.6	36.8	38.6	34.2	38.6
75-79	36.29998524180756	37.2	36.8	37.8	34.4	38.6
80-84	36.54303477648511	37.8	36.4	38.0	34.0	38.8
85-89	36.47668866300376	38.0	36.2	38.6	33.0	39.2
90-94	36.28337858110083	37.6	36.4	38.4	33.2	39.6
95-99	35.97465897677968	37.2	36.4	38.0	32.6	38.6
100	34.96361287858863	37.0	35.0	37.0	32.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
1	1.0
2	0.0
3	1.0
4	0.0
5	37.0
6	187.0
7	291.0
8	399.0
9	992.0
10	791.0
11	1109.0
12	1333.0
13	1649.0
14	2011.0
15	2491.0
16	2969.0
17	3461.0
18	4433.0
19	4839.0
20	4660.0
21	5644.0
22	6986.0
23	8726.0
24	11443.0
25	15967.0
26	21805.0
27	30346.0
28	41125.0
29	55399.0
30	72651.0
31	98361.0
32	133345.0
33	182331.0
34	274921.0
35	528797.0
36	1783456.0
37	5321878.0
38	2599.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.88207006264916	15.880292759324	21.145966963981632	40.09167021404521
2	19.51186119699013	46.90765396811302	17.012195355218022	16.568289479678832
3	21.787281248297454	32.37607499429104	28.82927485194377	17.007368905467736
4	33.61277256772238	23.6313669458702	20.483050437815827	22.272810048591595
5	22.190732676554326	26.108373984522043	25.21246169951508	26.48843163940855
6	22.288540154632877	27.6925648567586	25.423439013120543	24.595455975487983
7	22.85594458900794	26.210886713120697	26.32881080399354	24.604357893877822
8	22.561089833353424	25.825269166210713	27.459659844389012	24.15398115604685
9	22.71533045723356	28.197559005273032	25.990870931496744	23.096239605996665
10-14	23.348395201707483	26.157652625190202	25.89358364685468	24.60036852624764
15-19	22.4600430542697	26.42252398242187	26.88182733778487	24.23560562552357
20-24	22.69072791481808	25.372661168894496	26.85411604314379	25.08249487314364
25-29	22.731631899398796	26.350934577976126	27.572843343367893	23.34459017925718
30-34	22.52223057561096	27.346825184975295	26.842928890456268	23.28801534895748
35-39	22.904485241700232	26.609640229960483	27.74011126056172	22.745763267777566
40-44	22.480942207649935	27.248594479477507	27.10501454554691	23.165448767325646
45-49	22.706271120131603	27.562407390635578	27.44635338100272	22.284968108230096
50-54	22.758137656071106	27.296050644664184	27.435390992928095	22.51042070633661
55-59	22.60761508613266	27.779099932489636	27.322938587885016	22.29034639349268
60-64	22.421381478227204	27.33442970389727	27.9044768258794	22.339711991996126
65-69	22.88009200142465	27.686764880682663	27.819035116010106	21.614108001882588
70-74	22.346418850776402	27.815632477618706	27.303250782908954	22.534697888695934
75-79	22.8668022164701	27.862569436764616	27.08726311871676	22.183365228048533
80-84	22.446046397388177	27.851944195004	27.011211880168972	22.690797527438853
85-89	22.654373051559894	27.80420761867786	27.169736067635945	22.371683262126293
90-94	22.41310663190756	27.241122789897233	27.625228876701392	22.72054170149381
95-99	22.72210410395042	26.76890154231725	27.526060690036513	22.98293366369582
100	23.52520496701179	26.158748104616325	26.69905417351155	23.616992754860338
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	62.973740276694365
2	36.080150676668964
3	38.79465015376519
4	55.885582616313975
5	48.67916431596287
6	46.883996130120856
7	47.460302482885766
8	46.715070989400274
9	45.81157006323022
10-14	47.94876372795512
15-19	46.695648679793266
20-24	47.773222787961714
25-29	46.076222078655974
30-34	45.810245924568434
35-39	45.6502485094778
40-44	45.64639097497558
45-49	44.9912392283617
50-54	45.26855836240772
55-59	44.897961479625344
60-64	44.76109347022333
65-69	44.494200003307235
70-74	44.88111673947234
75-79	45.05016744451863
80-84	45.13684392482703
85-89	45.02605631368619
90-94	45.133648333401375
95-99	45.705037767646246
100	47.14219772187212
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	381.0
1	379.0
2	377.5
3	382.0
4	387.0
5	390.5
6	403.0
7	424.0
8	445.5
9	469.5
10	496.5
11	536.0
12	571.5
13	634.0
14	694.5
15	1053.0
16	1323.5
17	1388.0
18	1731.0
19	2819.0
20	4082.0
21	4862.5
22	7217.0
23	13856.5
24	26043.5
25	37450.5
26	48203.5
27	59229.0
28	70883.0
29	97771.5
30	130953.0
31	144608.5
32	158648.5
33	179854.0
34	213270.0
35	231149.5
36	216296.0
37	214556.5
38	244005.5
39	282442.0
40	321665.5
41	324699.5
42	305003.5
43	346461.5
44	379612.0
45	369458.5
46	334020.5
47	275301.5
48	264190.0
49	296429.5
50	323211.5
51	348773.5
52	303253.0
53	215792.0
54	186852.5
55	174645.0
56	139809.0
57	114522.0
58	141244.5
59	149299.0
60	137374.5
61	147477.5
62	127289.5
63	103207.0
64	84740.5
65	76996.5
66	102981.0
67	99585.0
68	64095.0
69	43492.5
70	35189.5
71	30862.0
72	25585.0
73	15634.5
74	10494.5
75	12890.0
76	12598.5
77	11669.5
78	8950.5
79	6070.0
80	5975.5
81	6825.0
82	4664.5
83	2528.5
84	2328.0
85	1349.0
86	2775.0
87	2221.0
88	638.5
89	516.0
90	397.5
91	365.0
92	285.5
93	228.0
94	211.5
95	203.0
96	190.5
97	179.5
98	174.5
99	171.5
100	168.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.038632576035933744
2	0.010490250558185652
3	0.0030601408082063706
4	0.002839906035043745
5	0.001182341246635109
6	0.002550178825494277
7	1.3910224339461406E-4
8	0.0011360029712273632
9	8.114308818538089E-5
10-14	3.7557737196410897E-4
15-19	0.011654545274975631
20-24	0.011583883173616718
25-29	0.008423080381256633
30-34	0.009780487317825088
35-39	0.006209132968918848
40-44	0.01027321224071447
45-49	0.009668299390674453
50-54	0.01000867976888482
55-59	0.009566411873262338
60-64	0.03804773714520948
65-69	0.02950991248367146
70-74	0.017870557842072584
75-79	0.03912759039466523
80-84	0.04494255230941171
85-89	0.012352797566469445
90-94	0.002597656303380452
95-99	0.006762899238688632
100	0.012012665142533433
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
0-1	376.0
2-3	12.0
4-5	200.0
6-7	108.0
8-9	6.0
10-11	15.0
12-13	7.0
14-15	2.0
16-17	25.0
18-19	135.0
20-21	913.0
22-23	436.0
24-25	1167.0
26-27	258.0
28-29	190.0
30-31	373.0
32-33	165.0
34-35	183.0
36-37	152.0
38-39	128.0
40-41	185.0
42-43	139.0
44-45	156.0
46-47	228.0
48-49	117.0
50-51	25698.0
52-53	940.0
54-55	28819.0
56-57	44835.0
58-59	90942.0
60-61	1986.0
62-63	37070.0
64-65	23042.0
66-67	18852.0
68-69	20068.0
70-71	40628.0
72-73	40500.0
74-75	16022.0
76-77	14950.0
78-79	5708.0
80-81	14246.0
82-83	28347.0
84-85	10419.0
86-87	6957.0
88-89	11579.0
90-91	28518.0
92-93	686.0
94-95	9306.0
96-97	193320.0
98-99	0.0
100-101	7908320.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	98.51550298733088
#Duplication Level	Relative count
1	100.0
2	13.622593188313006
3	5.097053266080547
4	2.6414416665365335
5	1.4210534534722115
6	0.8666864468978872
7	0.5442166247638084
8	0.3903993004044537
9	0.2998266627106204
10++	7.2200446616799665
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACAACTTGGCCACCGACAGCTGAAGGGCTTTTCACCTGTTGACACAATTGCCAGTCA	15139	0.17547511809420968	No Hit
TGACTGGCAATTGTGTCAACAGGTGAAAAGCCCTTCAGCTGTCGGTGGCCAAGTTGTC	15081	0.1748028440437794	No Hit
CATGCACTATCCAGGCGCCTTCACCTACTCCCCGACGCCGGTCACCTCGGGCATCGGCATCGG	14136	0.16384941339452727	No Hit
CCGATGCCGATGCCCGAGGTGACCGGCGTCGGGGAGTAGGTGAAGGCGCCTGGATAGTGCATG	13649	0.15820462955729364	No Hit
CATGTAATTCAGGCAGTTATGGGAGCCCTAGAGGGCCTGAGAGTTGCTATTGGAC	11581	0.13423458237988262	No Hit
GTCCAATAGCAACTCTCAGGCCCTCTAGGGCTCCCATAACTGCCTGAATTACATG	11529	0.13363185392087612	No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG	11393	0.13205548718193613	No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA	11275	0.1306877572172676	No Hit
GAGCTACCATCTGATCTGTCTGTCTTGACCACCCGGAGTCCCACTGTCCCCAGCCAGAATCCCAGTAGACTA	11138	0.12909979954642364	No Hit
TAGTCTACTGGGATTCTGGCTGGGGACAGTGGGACTCCGGGTGGTCAAGACAGACAGATCAGATGGTAGCTC	10984	0.1273147960332122	No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC	10654	0.12348978850490193	No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT	10627	0.12317683334349472	No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG	10612	0.12300296936493516	No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT	10572	0.12253933208877633	No Hit
ATCCTTCGAGGGCTTTGTCTGACAACTGTCTTATCTTCTTTTGTAAGTGG	10236	0.11864477896904224	No Hit
TAATTCTGAACTGGAAAAGCCCCAGAAAGTCCGGAAAGACAAGGAAGGAA	10155	0.11770591348482062	No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA	10125	0.11735818552770151	No Hit
CATGCTTTCCCTGGGTCTTCACGGATTGCTTTCCAAGCTGCCTTGTTGCG	10106	0.11713795782152608	No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC	10097	0.11703363943439034	No Hit
GCGACAGCTGGAAAGGTCCAGTTGCTGCTCTGCAGCAACCCCGCAACAAG	10091	0.11696409384296652	No Hit
TAATGAGCATGAAGTCACCACACGGAGGAAAATGTAAATGTGTAAACCTC	9724	0.11271022183420934	No Hit
ATTCAGCTGCCAAGTAACTTAGCACACCCATTCTTATAAGATAATGGCAA	9721	0.11267544903849741	No Hit
TAGCTGCTCCATTTTCATTTCACCACTTTGAAATCTTGGAACCACTGATG	9627	0.1115859014395242	No Hit
GAAGGGCAGGAGTTCCTTTGGTGACTTCACAGTGAAGTCTTGCCCTCTCT	9614	0.11143521932477259	No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG	9374	0.10865339566781965	No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA	9374	0.10865339566781965	No Hit
CATGGAATATATATTCTCCTGGCTGGTGCTTATGATTGCAAAACTTGGAT	9245	0.10715816545220747	No Hit
CACACTGTCATTAGTTGCAGCATCTCCTGTAGCAATTGGGCTAAATCTGT	9220	0.10686839215460821	No Hit
AGTTGACAACTTGGACTTGGCCAATACTGCATTCTAAATCACCACATCAT	9163	0.10620770903608187	No Hit
TAATCTGATGTGGCATTTTCGTCATCTGAAGCATGAGTGACAAGTTGGGA	9069	0.10511816143710866	No Hit
AGACTCTTACTCTTCTCAATCTTGCAGAGCTGAGCTTTGGCCTTTTTTAG	8699	0.10082951663263956	No Hit
TAAACATTCCACTTCCTCCATAGGCTCCATGTTGGCTCAGGCAGACAAGC	8647	0.10022678817363309	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	3589075	3.106052	4.582902	75-79
CCCAG	1772750	2.8741364	5.262086	2
CCCCA	1543995	2.4659572	6.483209	1
TGGGG	1365220	2.3363318	7.346469	3
CCTCC	1447180	2.33224	5.37907	6
CTGCC	1418685	2.320903	6.0127654	7
CCACC	1329515	2.1234052	6.799939	1
GAGGG	1193445	2.0240588	5.39377	8
TGGCT	1399275	1.9892449	6.0607696	3
CCCTG	1179585	1.9297465	5.4192066	1
TGCAG	1348205	1.8994592	5.8098807	3
GGCTC	1088355	1.8074316	7.0815325	1
GCCCA	1093970	1.7736393	5.382423	2
GCTCC	1002935	1.6407553	6.726835	2
TGCCC	947575	1.550189	8.85031	3
TAAAA	1816805	1.5166662	5.964537	1
CATGG	1040430	1.4658412	34.359364	1
ATGGA	1217520	1.455234	9.637361	2
TCAGG	1016300	1.4318448	5.393309	9
AGACA	1160665	1.3543543	5.1932783	2
GGCCC	690070	1.3387226	5.6222296	1
ATGGG	908595	1.2994652	10.826983	2
TGGGT	899025	1.2974099	5.707081	3
ATGTG	1075275	1.2968432	8.815206	2
ATGGC	917105	1.292091	10.4141445	2
ATGAA	1173180	1.171882	7.188404	2
ATGCC	829040	1.150613	7.8342338	2
TGAGC	803405	1.1319014	5.1781363	1
ATGTT	1084230	1.1027141	6.085433	2
ATGCT	908510	1.0793874	7.5212755	2
CATGA	894300	1.052979	21.295494	1
CATGT	885645	1.0522218	26.592321	1
ATGGT	834685	1.0066779	5.8968225	2
CATGC	696100	0.96610737	27.342167	1
ATGAC	796110	0.9373668	5.834309	2
ATGTA	865490	0.8723533	6.879848	2
ATGCA	688650	0.81083983	9.894211	2
TAAGT	741150	0.7470273	6.2101684	1
>>END_MODULE