FastQCFastQC Report
Wed 20 Mar 2013
8016-7-5.h.sapiens.aligned.bam

Summary

[OK] Basic Statistics

Measure Value
Filename 8016-7-5.h.sapiens.aligned.bam
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 8627434
Filtered Sequences 0
Sequence length 1-100
%GC 45

[WARN] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
GACAACTTGGCCACCGACAGCTGAAGGGCTTTTCACCTGTTGACACAATTGCCAGTCA 15139 0.17547511809420968 No Hit
TGACTGGCAATTGTGTCAACAGGTGAAAAGCCCTTCAGCTGTCGGTGGCCAAGTTGTC 15081 0.1748028440437794 No Hit
CATGCACTATCCAGGCGCCTTCACCTACTCCCCGACGCCGGTCACCTCGGGCATCGGCATCGG 14136 0.16384941339452727 No Hit
CCGATGCCGATGCCCGAGGTGACCGGCGTCGGGGAGTAGGTGAAGGCGCCTGGATAGTGCATG 13649 0.15820462955729364 No Hit
CATGTAATTCAGGCAGTTATGGGAGCCCTAGAGGGCCTGAGAGTTGCTATTGGAC 11581 0.13423458237988262 No Hit
GTCCAATAGCAACTCTCAGGCCCTCTAGGGCTCCCATAACTGCCTGAATTACATG 11529 0.13363185392087612 No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG 11393 0.13205548718193613 No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA 11275 0.1306877572172676 No Hit
GAGCTACCATCTGATCTGTCTGTCTTGACCACCCGGAGTCCCACTGTCCCCAGCCAGAATCCCAGTAGACTA 11138 0.12909979954642364 No Hit
TAGTCTACTGGGATTCTGGCTGGGGACAGTGGGACTCCGGGTGGTCAAGACAGACAGATCAGATGGTAGCTC 10984 0.1273147960332122 No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC 10654 0.12348978850490193 No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT 10627 0.12317683334349472 No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG 10612 0.12300296936493516 No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT 10572 0.12253933208877633 No Hit
ATCCTTCGAGGGCTTTGTCTGACAACTGTCTTATCTTCTTTTGTAAGTGG 10236 0.11864477896904224 No Hit
TAATTCTGAACTGGAAAAGCCCCAGAAAGTCCGGAAAGACAAGGAAGGAA 10155 0.11770591348482062 No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA 10125 0.11735818552770151 No Hit
CATGCTTTCCCTGGGTCTTCACGGATTGCTTTCCAAGCTGCCTTGTTGCG 10106 0.11713795782152608 No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC 10097 0.11703363943439034 No Hit
GCGACAGCTGGAAAGGTCCAGTTGCTGCTCTGCAGCAACCCCGCAACAAG 10091 0.11696409384296652 No Hit
TAATGAGCATGAAGTCACCACACGGAGGAAAATGTAAATGTGTAAACCTC 9724 0.11271022183420934 No Hit
ATTCAGCTGCCAAGTAACTTAGCACACCCATTCTTATAAGATAATGGCAA 9721 0.11267544903849741 No Hit
TAGCTGCTCCATTTTCATTTCACCACTTTGAAATCTTGGAACCACTGATG 9627 0.1115859014395242 No Hit
GAAGGGCAGGAGTTCCTTTGGTGACTTCACAGTGAAGTCTTGCCCTCTCT 9614 0.11143521932477259 No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG 9374 0.10865339566781965 No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA 9374 0.10865339566781965 No Hit
CATGGAATATATATTCTCCTGGCTGGTGCTTATGATTGCAAAACTTGGAT 9245 0.10715816545220747 No Hit
CACACTGTCATTAGTTGCAGCATCTCCTGTAGCAATTGGGCTAAATCTGT 9220 0.10686839215460821 No Hit
AGTTGACAACTTGGACTTGGCCAATACTGCATTCTAAATCACCACATCAT 9163 0.10620770903608187 No Hit
TAATCTGATGTGGCATTTTCGTCATCTGAAGCATGAGTGACAAGTTGGGA 9069 0.10511816143710866 No Hit
AGACTCTTACTCTTCTCAATCTTGCAGAGCTGAGCTTTGGCCTTTTTTAG 8699 0.10082951663263956 No Hit
TAAACATTCCACTTCCTCCATAGGCTCCATGTTGGCTCAGGCAGACAAGC 8647 0.10022678817363309 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 3589075 3.106052 4.582902 75-79
CCCAG 1772750 2.8741364 5.262086 2
CCCCA 1543995 2.4659572 6.483209 1
TGGGG 1365220 2.3363318 7.346469 3
CCTCC 1447180 2.33224 5.37907 6
CTGCC 1418685 2.320903 6.0127654 7
CCACC 1329515 2.1234052 6.799939 1
GAGGG 1193445 2.0240588 5.39377 8
TGGCT 1399275 1.9892449 6.0607696 3
CCCTG 1179585 1.9297465 5.4192066 1
TGCAG 1348205 1.8994592 5.8098807 3
GGCTC 1088355 1.8074316 7.0815325 1
GCCCA 1093970 1.7736393 5.382423 2
GCTCC 1002935 1.6407553 6.726835 2
TGCCC 947575 1.550189 8.85031 3
TAAAA 1816805 1.5166662 5.964537 1
CATGG 1040430 1.4658412 34.359364 1
ATGGA 1217520 1.455234 9.637361 2
TCAGG 1016300 1.4318448 5.393309 9
AGACA 1160665 1.3543543 5.1932783 2
GGCCC 690070 1.3387226 5.6222296 1
ATGGG 908595 1.2994652 10.826983 2
TGGGT 899025 1.2974099 5.707081 3
ATGTG 1075275 1.2968432 8.815206 2
ATGGC 917105 1.292091 10.4141445 2
ATGAA 1173180 1.171882 7.188404 2
ATGCC 829040 1.150613 7.8342338 2
TGAGC 803405 1.1319014 5.1781363 1
ATGTT 1084230 1.1027141 6.085433 2
ATGCT 908510 1.0793874 7.5212755 2
CATGA 894300 1.052979 21.295494 1
CATGT 885645 1.0522218 26.592321 1
ATGGT 834685 1.0066779 5.8968225 2
CATGC 696100 0.96610737 27.342167 1
ATGAC 796110 0.9373668 5.834309 2
ATGTA 865490 0.8723533 6.879848 2
ATGCA 688650 0.81083983 9.894211 2
TAAGT 741150 0.7470273 6.2101684 1
Produced by FastQC (version 0.10.0)