##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	8016-7-6.h.sapiens.aligned.bam	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	8890070	
Filtered Sequences	0	
Sequence length	1-100	
%GC	45	
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.830066917358355	24.0	24.0	32.0	24.0	32.0
2	31.015920193722035	31.0	31.0	32.0	30.0	32.0
3	32.708793718989114	34.0	33.0	34.0	30.0	34.0
4	34.264758389446726	35.0	35.0	35.0	29.0	37.0
5	35.39308327339855	36.0	36.0	36.0	34.0	36.0
6	35.576957087284136	36.0	36.0	37.0	34.0	37.0
7	35.93369495256757	37.0	35.0	37.0	33.0	38.0
8	36.27561722367028	37.0	36.0	37.0	34.0	38.0
9	36.50979720436676	37.0	37.0	38.0	35.0	38.0
10-14	36.78817965796994	37.6	36.8	38.0	34.8	38.2
15-19	37.2908817807976	38.0	37.0	38.8	34.8	39.2
20-24	36.91916094204622	37.8	37.0	38.2	35.6	38.6
25-29	37.049957270283656	38.0	36.8	38.6	34.2	39.0
30-34	37.050044100675606	37.8	36.8	38.8	35.4	39.2
35-39	36.96705475287339	38.0	37.2	38.6	33.8	39.2
40-44	36.84597407995812	38.0	36.8	38.2	33.6	39.2
45-49	36.69887537956627	37.4	37.0	38.4	33.8	39.2
50-54	36.9443084409787	38.2	37.2	38.2	34.0	39.0
55-59	37.0049633522971	38.0	37.2	38.4	34.0	38.8
60-64	36.49276492184525	37.4	36.4	38.0	33.8	38.4
65-69	36.590590061008605	37.8	36.4	38.4	34.2	38.8
70-74	36.6388130851148	37.6	36.8	38.6	34.2	38.6
75-79	36.303013887735005	37.2	36.8	37.8	34.4	38.6
80-84	36.55071730730858	37.8	36.4	38.0	34.4	38.8
85-89	36.485782657919934	38.0	36.2	38.6	33.0	39.2
90-94	36.28074627990421	37.6	36.4	38.4	33.2	39.6
95-99	35.98360294273857	37.2	36.4	38.0	32.6	38.6
100	34.979486299012876	37.0	35.0	37.0	32.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
1	3.0
2	0.0
3	11.0
4	1.0
5	26.0
6	152.0
7	283.0
8	460.0
9	1625.0
10	864.0
11	1131.0
12	1377.0
13	1758.0
14	2173.0
15	2711.0
16	3202.0
17	3782.0
18	4771.0
19	4826.0
20	5268.0
21	6013.0
22	7209.0
23	9068.0
24	12065.0
25	16531.0
26	22597.0
27	31464.0
28	42516.0
29	57115.0
30	76466.0
31	101719.0
32	135966.0
33	186644.0
34	280843.0
35	542254.0
36	1813208.0
37	5510662.0
38	3306.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.35091437502701	16.69855334994742	21.698827950561085	39.251704324464484
2	18.86041310525604	49.02415567425112	15.640151582487052	16.47527963800579
3	21.314055399702454	32.845107367636615	28.82913744983525	17.01169978282568
4	34.023326839342985	23.691368317929747	20.335141202945618	21.950163639781653
5	22.181348862859842	26.294761597362587	25.27514140984814	26.24874812992943
6	22.179816316411983	27.975975978678818	25.266860754033583	24.577346950875615
7	22.80884169891989	26.441100754928996	26.679678209573293	24.07037933657782
8	22.80840828137411	25.710399420366812	27.639603617307092	23.841588680951983
9	22.637033010192837	28.128535116791966	25.90381703453351	23.330614838481694
10-14	23.213324355097306	26.046619204061646	26.090905854842838	24.649150585998207
15-19	22.3672909339624	26.369353296600394	26.96001752076574	24.303338248671462
20-24	22.578464690107236	25.51698540434603	26.900677098567293	25.003872806979434
25-29	22.524766570640097	26.541249163804633	27.644179288753705	23.289804976801566
30-34	22.550020947320647	27.155343947167935	27.025369893109545	23.26926521240187
35-39	22.775794820772855	26.513980203482152	27.830631022958823	22.87959395278617
40-44	22.488631827998454	27.062824298110545	27.356988981860482	23.091554892030523
45-49	22.718356252291194	27.53084633232625	27.566933266968213	22.183864148414344
50-54	22.88097645252256	27.284617247684718	27.391396991721216	22.443009308071503
55-59	22.584152759120368	27.667951635450134	27.230142059069152	22.51775354636035
60-64	22.47339217876508	27.247615673543457	27.973429292938366	22.305562854753095
65-69	22.873633624073932	27.761387551417087	27.681079321089967	21.683899503419017
70-74	22.27178874012904	27.88855069885391	27.24029274970267	22.59936781131438
75-79	22.885618611794605	27.827981829785408	27.153813056589243	22.13258650183074
80-84	22.39369028787401	27.71898212976685	27.313609655463537	22.5737179268956
85-89	22.715633176519507	27.933593540249596	27.005417704170178	22.345355579060723
90-94	22.387707789394096	27.41642168361096	27.403909731397707	22.79196079559724
95-99	22.62170977391202	26.73072769604775	27.712888629763743	22.934673900276486
100	23.89470730575448	26.423347687256353	26.18232474763873	23.499620259350433
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	61.602618699491494
2	35.33569274326183
3	38.325755182528134
4	55.973490479124635
5	48.430096992789274
6	46.7571632672876
7	46.8792210354977
8	46.64999696232609
9	45.96764784867453
10-14	47.86247494109551
15-19	46.67062918263387
20-24	47.58233749708668
25-29	45.81457154744166
30-34	45.819286159722516
35-39	45.655388773559025
40-44	45.580186720028976
45-49	44.90222040070554
50-54	45.32398576059406
55-59	45.101906305480725
60-64	44.77895503351817
65-69	44.557533127492945
70-74	44.87115655144342
75-79	45.01820511362534
80-84	44.96740821476961
85-89	45.060988755580226
90-94	45.17966858499133
95-99	45.55638367418851
100	47.394327565104916
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1044.0
1	1046.5
2	1048.0
3	1048.0
4	1051.0
5	1055.0
6	1069.5
7	1082.5
8	1098.0
9	1126.0
10	1161.0
11	1214.0
12	1252.0
13	1291.0
14	1347.0
15	1560.0
16	1764.0
17	1893.0
18	2277.0
19	2875.0
20	4102.0
21	5460.5
22	8112.0
23	14308.0
24	25816.0
25	36840.0
26	46982.0
27	60075.0
28	70388.5
29	92231.5
30	122574.0
31	140941.0
32	161765.0
33	186733.5
34	216862.0
35	231947.0
36	222637.5
37	224894.5
38	261004.0
39	300381.5
40	334451.5
41	348988.0
42	339370.5
43	377592.5
44	411777.5
45	391872.0
46	347348.5
47	289353.5
48	284580.0
49	317285.0
50	335812.5
51	354396.0
52	307051.0
53	223756.0
54	187904.5
55	169641.0
56	140807.0
57	113979.5
58	139741.5
59	149144.0
60	138025.0
61	144025.0
62	123225.5
63	102351.0
64	83933.0
65	77439.5
66	100640.0
67	100061.5
68	63070.0
69	43579.5
70	36317.0
71	30937.0
72	27800.0
73	16432.0
74	10058.0
75	12654.5
76	12423.0
77	10691.5
78	7914.5
79	6806.5
80	6567.0
81	6142.5
82	4179.0
83	2773.0
84	2630.5
85	1608.5
86	3066.0
87	2417.5
88	762.0
89	618.0
90	508.0
91	463.5
92	373.5
93	296.5
94	266.5
95	255.5
96	242.5
97	228.5
98	232.5
99	231.0
100	228.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03873985244210676
2	0.010428590312210844
3	0.003408701639045496
4	0.0029699645460482317
5	0.001428759626267982
6	0.002891313392046121
7	2.3625929975668665E-4
8	0.0014850629666697869
9	9.000388704287166E-5
10-14	4.252688447256819E-4
15-19	0.01244994959379255
20-24	0.012216813783842971
25-29	0.008434140943231005
30-34	0.009880961213962104
35-39	0.006260708232034098
40-44	0.010517887728868225
45-49	0.010110978177562966
50-54	0.010185767131696326
55-59	0.010261263641080143
60-64	0.039142314218802016
65-69	0.030404983857323452
70-74	0.01916482924791948
75-79	0.03941965783784107
80-84	0.045454919240247825
85-89	0.012726872765736011
90-94	0.002784326078167156
95-99	0.00647127036059657
100	0.011989083198849048
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
0-1	1045.0
2-3	30.0
4-5	301.0
6-7	182.0
8-9	9.0
10-11	8.0
12-13	4.0
14-15	28.0
16-17	84.0
18-19	244.0
20-21	2700.0
22-23	683.0
24-25	1590.0
26-27	469.0
28-29	443.0
30-31	693.0
32-33	232.0
34-35	415.0
36-37	239.0
38-39	258.0
40-41	385.0
42-43	199.0
44-45	255.0
46-47	258.0
48-49	228.0
50-51	23473.0
52-53	850.0
54-55	26575.0
56-57	42019.0
58-59	92955.0
60-61	1906.0
62-63	34760.0
64-65	22176.0
66-67	19986.0
68-69	20940.0
70-71	41017.0
72-73	38658.0
74-75	17249.0
76-77	14796.0
78-79	4841.0
80-81	12128.0
82-83	21265.0
84-85	12932.0
86-87	6162.0
88-89	9135.0
90-91	28289.0
92-93	3200.0
94-95	9144.0
96-97	208870.0
98-99	0.0
100-101	8165762.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	98.54010115558171
#Duplication Level	Relative count
1	100.0
2	12.761006580062565
3	5.087605751005501
4	3.0257500809024087
5	1.766754503567411
6	1.1719291757200314
7	0.7050067033424252
8	0.4730864654122941
9	0.37061008121060823
10++	7.681876319479759
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACAACTTGGCCACCGACAGCTGAAGGGCTTTTCACCTGTTGACACAATTGCCAGTCA	18429	0.2072987051845486	No Hit
TGACTGGCAATTGTGTCAACAGGTGAAAAGCCCTTCAGCTGTCGGTGGCCAAGTTGTC	18387	0.20682626795964484	No Hit
ATCCTTCGAGGGCTTTGTCTGACAACTGTCTTATCTTCTTTTGTAAGTGG	17287	0.1944529120693088	No Hit
TAATTCTGAACTGGAAAAGCCCCAGAAAGTCCGGAAAGACAAGGAAGGAA	17150	0.19291186683569422	No Hit
AGAAGAGGGCTCCTTTTGTGCATATCAAACCTTGTTCCAGAATGGAGTGG	15089	0.16972869729934634	No Hit
GACCCCCTCCACTAACTCCCGAGGACGTTGGCTTTGCATCTGGTTTTTCT	15031	0.16907628398876498	No Hit
AGACTCTTACTCTTCTCAATCTTGCAGAGCTGAGCTTTGGCCTTTTTTAG	14896	0.1675577357658601	No Hit
TAAACATTCCACTTCCTCCATAGGCTCCATGTTGGCTCAGGCAGACAAGC	14832	0.16683783142314965	No Hit
GATCCAAAACTTCTCTCCGACACTGAATACCTTCTGCTTCTCCTATCATT	14642	0.16470061540572797	No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT	14428	0.16229343525978984	No Hit
CATGCACTATCCAGGCGCCTTCACCTACTCCCCGACGCCGGTCACCTCGGGCATCGGCATCGG	14412	0.16211345917411224	No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT	14313	0.160999857143982	No Hit
CCGATGCCGATGCCCGAGGTGACCGGCGTCGGGGAGTAGGTGAAGGCGCCTGGATAGTGCATG	13931	0.15670292809842892	No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG	13066	0.14697297096648285	No Hit
GACCACACACCTTGGCTTATGCCTCCAACAATCCCCTTAGCATCACCATT	13065	0.14696172246112799	No Hit
TGACCTAGAATGCTTTAAAGAAGTCCACCTAAATGTCGGTTCTCGCAAAA	13011	0.14635430317196602	No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA	12856	0.14461078484196413	No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG	11628	0.13079762026620714	No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC	11622	0.130730129234078	No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT	11392	0.1281429730024623	No Hit
GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG	11326	0.12740057164904214	No Hit
CATGAACTGCCTGTCAACAGCTAACTATTTATGTAAATCAGTCTTACCAG	10850	0.12204628310013307	No Hit
GAGCTACCATCTGATCTGTCTGTCTTGACCACCCGGAGTCCCACTGTCCCCAGCCAGAATCCCAGTAGACTA	10755	0.12097767509142224	No Hit
CAGAAGCTGCACTTTATAAGAATCTTCTGCATTCAAAGGAGTCTTCCTGG	10742	0.12083144452180916	No Hit
TAGTCTACTGGGATTCTGGCTGGGGACAGTGGGACTCCGGGTGGTCAAGACAGACAGATCAGATGGTAGCTC	10576	0.1189641926329039	No Hit
CATGCTTTCCCTGGGTCTTCACGGATTGCTTTCCAAGCTGCCTTGTTGCG	10482	0.1179068331295479	No Hit
GCGACAGCTGGAAAGGTCCAGTTGCTGCTCTGCAGCAACCCCGCAACAAG	10410	0.11709694074399865	No Hit
CATGGAAAGCCCCTGTTTCATACTGACCAAAACTCAGCCTGTTTCTGGGA	10349	0.11641078191735274	No Hit
TATGAAGTACAGTGGAAGGTTGTTGAGGAGATAAATGGAAACAATTATGT	10306	0.11592709618709414	No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA	10042	0.11295749077341348	No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC	10028	0.11280001169844556	No Hit
CATGTAATTCAGGCAGTTATGGGAGCCCTAGAGGGCCTGAGAGTTGCTATTGGAC	10027	0.11278876319309072	No Hit
GTCCAATAGCAACTCTCAGGCCCTCTAGGGCTCCCATAACTGCCTGAATTACATG	9973	0.11218134390392877	No Hit
AGCAATTCCATTTATGTGTTTGTTAGAGGTAAATGCTTGGCTTTCTGCAGTGCTGTGCTTTCAAGAATTTA	9874	0.11106774187379853	No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA	9840	0.11068529269173358	No Hit
TAAATTCTTGAAAGCACAGCACTGCAGAAAGCCAAGCATTTACCTCTAACAAACACATAAATGGAATTGCT	9831	0.11058405614353993	No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC	9821	0.11047157108999142	No Hit
CATGGAATATATATTCTCCTGGCTGGTGCTTATGATTGCAAAACTTGGAT	9794	0.11016786144541044	No Hit
TGATTTTTCTCTGCAGATTCCCCAGACTGAGTCAGGGAATGAGAAGGAAA	9744	0.10960543617766788	No Hit
CACACTGTCATTAGTTGCAGCATCTCCTGTAGCAATTGGGCTAAATCTGT	9737	0.10952669664018393	No Hit
CAACACCCAACACTTATAAGCGGAAGAACACAGAAACAGCTCTAGACAAC	9584	0.10780567532089173	No Hit
GAGCTCTCACCTCTCACCCCCCCGTCTTCTGTCTCTTCCTCGTTAAGCAT	9471	0.10653459421579357	No Hit
CATGTAGACTTACCTTTCCTCATAGAGCTATCCTGGTTAATAACAGGCCA	9457	0.10637711514082566	No Hit
ACACTTACACCAAGGAATCTCCCAGGCATTATCAAAGCTACAGGAGACAA	9415	0.10590467791592192	No Hit
GAAGGGCAGGAGTTCCTTTGGTGACTTCACAGTGAAGTCTTGCCCTCTCT	9274	0.10431863866088795	No Hit
TAGCTGCTCCATTTTCATTTCACCACTTTGAAATCTTGGAACCACTGATG	9272	0.10429614165017823	No Hit
TAAGTATACCTTGGCTTCGTTCAGTTATAATTTCAACATGTATGGTTGTT	9045	0.10174273093462706	No Hit
ATCCGGGGTCCTCGCACAGAGGTCTCTGATGAGTCACTTTCTTGACCCTG	9024	0.1015065123221752	No Hit
CATGGGGTGGGTGCTTGCAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTT	8954	0.10071911694733562	No Hit
GGCTGCTTGTTGTACGTGGCCTGGTGGAACGCACTGCAAAACGAGCTCAG	8898	0.10008920064746396	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	3732695	3.1129744	4.71728	75-79
GGAGG	1493205	2.4713368	5.0017805	4
CCCCA	1551960	2.4104571	5.6820383	1
CCAGC	1509310	2.3817477	5.8860292	5
CCTCC	1517730	2.3781295	6.345255	6
TGGGG	1398195	2.3345454	8.703637	3
GGGGG	1163140	2.3101327	5.7464767	4
CTGCC	1428055	2.2734444	5.79266	7
CTCCA	1645785	2.1833332	5.4600205	7
TGGAG	1519405	2.114052	5.050632	3
CCACC	1336145	2.0752602	6.0976553	1
CTGGA	1506775	2.0634413	5.2120547	8
TGGAA	1762090	2.043044	5.07154	3
TGGCT	1427030	1.9715095	5.981999	3
GAGGG	1186825	1.964261	6.1084905	8
TGCAG	1428995	1.9569259	5.4017096	3
CCCTG	1221710	1.9449459	5.349376	1
GGCTC	1129465	1.8268824	7.098622	1
GCCCA	1126025	1.7769097	5.426999	4
GCTCC	1026600	1.6343333	6.571143	2
TGCCC	974540	1.5514544	9.219851	3
TAAAA	1839365	1.4809235	7.0018063	1
ATGGA	1273980	1.4771079	10.93803	2
CATGG	1061795	1.4540668	37.427082	1
TGAGG	1041610	1.449263	5.253697	1
ACAGC	1077395	1.4394554	5.0504365	7
TCAGG	1046940	1.4337237	5.174131	9
AGACA	1206065	1.3642687	5.3752847	2
GGCCC	716595	1.3570117	5.8493075	1
TGGGT	904845	1.2701013	5.6000533	3
ATGTG	1085205	1.2693561	9.999267	2
ATGGG	895370	1.2457893	11.439032	2
ATGGC	907790	1.2431659	10.542864	2
ATGAA	1256215	1.2137274	8.672836	2
TGAGC	846640	1.1594244	5.2275805	1
ATGCC	854705	1.1520239	8.301504	2
ATGTT	1091965	1.073767	6.4252777	2
CATGT	928210	1.0686109	28.363405	1
ATGCT	919685	1.0587962	7.728737	2
CATGA	927325	1.0582377	24.250275	1
ATGGT	866495	1.0135328	6.6553636	2
CATGC	699285	0.9425394	28.04537	1
ATGAC	818940	0.93455166	6.2806396	2
ATGTA	874465	0.85235727	6.628797	2
ATGCA	712420	0.81299394	9.864366	2
TAAGT	751900	0.7328909	6.6031933	1
TAAAC	731225	0.6953602	5.5790577	1
>>END_MODULE