FastQCFastQC Report
Wed 20 Mar 2013
8016-7-6.h.sapiens.aligned.bam

Summary

[OK] Basic Statistics

Measure Value
Filename 8016-7-6.h.sapiens.aligned.bam
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 8890070
Filtered Sequences 0
Sequence length 1-100
%GC 45

[WARN] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
GACAACTTGGCCACCGACAGCTGAAGGGCTTTTCACCTGTTGACACAATTGCCAGTCA 18429 0.2072987051845486 No Hit
TGACTGGCAATTGTGTCAACAGGTGAAAAGCCCTTCAGCTGTCGGTGGCCAAGTTGTC 18387 0.20682626795964484 No Hit
ATCCTTCGAGGGCTTTGTCTGACAACTGTCTTATCTTCTTTTGTAAGTGG 17287 0.1944529120693088 No Hit
TAATTCTGAACTGGAAAAGCCCCAGAAAGTCCGGAAAGACAAGGAAGGAA 17150 0.19291186683569422 No Hit
AGAAGAGGGCTCCTTTTGTGCATATCAAACCTTGTTCCAGAATGGAGTGG 15089 0.16972869729934634 No Hit
GACCCCCTCCACTAACTCCCGAGGACGTTGGCTTTGCATCTGGTTTTTCT 15031 0.16907628398876498 No Hit
AGACTCTTACTCTTCTCAATCTTGCAGAGCTGAGCTTTGGCCTTTTTTAG 14896 0.1675577357658601 No Hit
TAAACATTCCACTTCCTCCATAGGCTCCATGTTGGCTCAGGCAGACAAGC 14832 0.16683783142314965 No Hit
GATCCAAAACTTCTCTCCGACACTGAATACCTTCTGCTTCTCCTATCATT 14642 0.16470061540572797 No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT 14428 0.16229343525978984 No Hit
CATGCACTATCCAGGCGCCTTCACCTACTCCCCGACGCCGGTCACCTCGGGCATCGGCATCGG 14412 0.16211345917411224 No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT 14313 0.160999857143982 No Hit
CCGATGCCGATGCCCGAGGTGACCGGCGTCGGGGAGTAGGTGAAGGCGCCTGGATAGTGCATG 13931 0.15670292809842892 No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG 13066 0.14697297096648285 No Hit
GACCACACACCTTGGCTTATGCCTCCAACAATCCCCTTAGCATCACCATT 13065 0.14696172246112799 No Hit
TGACCTAGAATGCTTTAAAGAAGTCCACCTAAATGTCGGTTCTCGCAAAA 13011 0.14635430317196602 No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA 12856 0.14461078484196413 No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG 11628 0.13079762026620714 No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC 11622 0.130730129234078 No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT 11392 0.1281429730024623 No Hit
GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG 11326 0.12740057164904214 No Hit
CATGAACTGCCTGTCAACAGCTAACTATTTATGTAAATCAGTCTTACCAG 10850 0.12204628310013307 No Hit
GAGCTACCATCTGATCTGTCTGTCTTGACCACCCGGAGTCCCACTGTCCCCAGCCAGAATCCCAGTAGACTA 10755 0.12097767509142224 No Hit
CAGAAGCTGCACTTTATAAGAATCTTCTGCATTCAAAGGAGTCTTCCTGG 10742 0.12083144452180916 No Hit
TAGTCTACTGGGATTCTGGCTGGGGACAGTGGGACTCCGGGTGGTCAAGACAGACAGATCAGATGGTAGCTC 10576 0.1189641926329039 No Hit
CATGCTTTCCCTGGGTCTTCACGGATTGCTTTCCAAGCTGCCTTGTTGCG 10482 0.1179068331295479 No Hit
GCGACAGCTGGAAAGGTCCAGTTGCTGCTCTGCAGCAACCCCGCAACAAG 10410 0.11709694074399865 No Hit
CATGGAAAGCCCCTGTTTCATACTGACCAAAACTCAGCCTGTTTCTGGGA 10349 0.11641078191735274 No Hit
TATGAAGTACAGTGGAAGGTTGTTGAGGAGATAAATGGAAACAATTATGT 10306 0.11592709618709414 No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA 10042 0.11295749077341348 No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC 10028 0.11280001169844556 No Hit
CATGTAATTCAGGCAGTTATGGGAGCCCTAGAGGGCCTGAGAGTTGCTATTGGAC 10027 0.11278876319309072 No Hit
GTCCAATAGCAACTCTCAGGCCCTCTAGGGCTCCCATAACTGCCTGAATTACATG 9973 0.11218134390392877 No Hit
AGCAATTCCATTTATGTGTTTGTTAGAGGTAAATGCTTGGCTTTCTGCAGTGCTGTGCTTTCAAGAATTTA 9874 0.11106774187379853 No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA 9840 0.11068529269173358 No Hit
TAAATTCTTGAAAGCACAGCACTGCAGAAAGCCAAGCATTTACCTCTAACAAACACATAAATGGAATTGCT 9831 0.11058405614353993 No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC 9821 0.11047157108999142 No Hit
CATGGAATATATATTCTCCTGGCTGGTGCTTATGATTGCAAAACTTGGAT 9794 0.11016786144541044 No Hit
TGATTTTTCTCTGCAGATTCCCCAGACTGAGTCAGGGAATGAGAAGGAAA 9744 0.10960543617766788 No Hit
CACACTGTCATTAGTTGCAGCATCTCCTGTAGCAATTGGGCTAAATCTGT 9737 0.10952669664018393 No Hit
CAACACCCAACACTTATAAGCGGAAGAACACAGAAACAGCTCTAGACAAC 9584 0.10780567532089173 No Hit
GAGCTCTCACCTCTCACCCCCCCGTCTTCTGTCTCTTCCTCGTTAAGCAT 9471 0.10653459421579357 No Hit
CATGTAGACTTACCTTTCCTCATAGAGCTATCCTGGTTAATAACAGGCCA 9457 0.10637711514082566 No Hit
ACACTTACACCAAGGAATCTCCCAGGCATTATCAAAGCTACAGGAGACAA 9415 0.10590467791592192 No Hit
GAAGGGCAGGAGTTCCTTTGGTGACTTCACAGTGAAGTCTTGCCCTCTCT 9274 0.10431863866088795 No Hit
TAGCTGCTCCATTTTCATTTCACCACTTTGAAATCTTGGAACCACTGATG 9272 0.10429614165017823 No Hit
TAAGTATACCTTGGCTTCGTTCAGTTATAATTTCAACATGTATGGTTGTT 9045 0.10174273093462706 No Hit
ATCCGGGGTCCTCGCACAGAGGTCTCTGATGAGTCACTTTCTTGACCCTG 9024 0.1015065123221752 No Hit
CATGGGGTGGGTGCTTGCAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTT 8954 0.10071911694733562 No Hit
GGCTGCTTGTTGTACGTGGCCTGGTGGAACGCACTGCAAAACGAGCTCAG 8898 0.10008920064746396 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 3732695 3.1129744 4.71728 75-79
GGAGG 1493205 2.4713368 5.0017805 4
CCCCA 1551960 2.4104571 5.6820383 1
CCAGC 1509310 2.3817477 5.8860292 5
CCTCC 1517730 2.3781295 6.345255 6
TGGGG 1398195 2.3345454 8.703637 3
GGGGG 1163140 2.3101327 5.7464767 4
CTGCC 1428055 2.2734444 5.79266 7
CTCCA 1645785 2.1833332 5.4600205 7
TGGAG 1519405 2.114052 5.050632 3
CCACC 1336145 2.0752602 6.0976553 1
CTGGA 1506775 2.0634413 5.2120547 8
TGGAA 1762090 2.043044 5.07154 3
TGGCT 1427030 1.9715095 5.981999 3
GAGGG 1186825 1.964261 6.1084905 8
TGCAG 1428995 1.9569259 5.4017096 3
CCCTG 1221710 1.9449459 5.349376 1
GGCTC 1129465 1.8268824 7.098622 1
GCCCA 1126025 1.7769097 5.426999 4
GCTCC 1026600 1.6343333 6.571143 2
TGCCC 974540 1.5514544 9.219851 3
TAAAA 1839365 1.4809235 7.0018063 1
ATGGA 1273980 1.4771079 10.93803 2
CATGG 1061795 1.4540668 37.427082 1
TGAGG 1041610 1.449263 5.253697 1
ACAGC 1077395 1.4394554 5.0504365 7
TCAGG 1046940 1.4337237 5.174131 9
AGACA 1206065 1.3642687 5.3752847 2
GGCCC 716595 1.3570117 5.8493075 1
TGGGT 904845 1.2701013 5.6000533 3
ATGTG 1085205 1.2693561 9.999267 2
ATGGG 895370 1.2457893 11.439032 2
ATGGC 907790 1.2431659 10.542864 2
ATGAA 1256215 1.2137274 8.672836 2
TGAGC 846640 1.1594244 5.2275805 1
ATGCC 854705 1.1520239 8.301504 2
ATGTT 1091965 1.073767 6.4252777 2
CATGT 928210 1.0686109 28.363405 1
ATGCT 919685 1.0587962 7.728737 2
CATGA 927325 1.0582377 24.250275 1
ATGGT 866495 1.0135328 6.6553636 2
CATGC 699285 0.9425394 28.04537 1
ATGAC 818940 0.93455166 6.2806396 2
ATGTA 874465 0.85235727 6.628797 2
ATGCA 712420 0.81299394 9.864366 2
TAAGT 751900 0.7328909 6.6031933 1
TAAAC 731225 0.6953602 5.5790577 1
Produced by FastQC (version 0.10.0)