##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	8016-7-7.h.sapiens.aligned.bam	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	6722214	
Filtered Sequences	0	
Sequence length	1-100	
%GC	46	
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.813893904597503	24.0	24.0	32.0	24.0	32.0
2	30.996299186183947	31.0	31.0	32.0	30.0	32.0
3	32.681936016928226	34.0	33.0	34.0	30.0	34.0
4	34.24823657997005	35.0	35.0	35.0	29.0	37.0
5	35.373557032662454	36.0	36.0	36.0	34.0	36.0
6	35.551545711571805	36.0	36.0	37.0	34.0	37.0
7	35.904869554946046	36.0	35.0	37.0	33.0	38.0
8	36.244928067694694	37.0	36.0	37.0	34.0	38.0
9	36.48256568597333	37.0	37.0	38.0	35.0	38.0
10-14	36.752206900075116	37.6	36.8	38.0	34.4	38.2
15-19	37.25188387403927	38.0	37.0	38.8	34.6	39.2
20-24	36.87451279552812	37.8	36.6	38.2	35.0	38.6
25-29	36.99524905510147	38.0	36.8	38.6	34.2	39.0
30-34	36.98879864588979	37.8	36.8	38.6	35.2	39.2
35-39	36.89812349411461	38.0	37.2	38.4	33.6	39.2
40-44	36.765936637252096	38.0	36.6	38.2	33.6	39.2
45-49	36.6323198914088	37.2	37.0	38.0	33.8	39.2
50-54	36.87757272055347	38.2	37.0	38.2	34.0	39.0
55-59	36.93144614565422	38.0	37.2	38.4	33.0	38.8
60-64	36.42423399432346	37.4	36.4	38.0	33.2	38.4
65-69	36.51393476031461	37.8	36.4	38.0	33.4	38.8
70-74	36.56175966921963	37.6	36.8	38.6	34.2	38.6
75-79	36.22382104708416	37.2	36.6	37.8	34.2	38.6
80-84	36.46605009379353	37.8	36.4	38.0	33.4	38.8
85-89	36.383740028578806	37.8	36.2	38.6	33.0	39.2
90-94	36.18187764154891	37.6	36.4	38.4	33.2	39.6
95-99	35.87243268592873	37.2	36.2	38.0	32.4	38.6
100	34.86137777891299	37.0	35.0	37.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
1	1.0
2	0.0
3	3.0
4	1.0
5	38.0
6	169.0
7	292.0
8	399.0
9	676.0
10	730.0
11	969.0
12	1182.0
13	1482.0
14	1754.0
15	2061.0
16	2479.0
17	3025.0
18	3947.0
19	3898.0
20	3860.0
21	4694.0
22	5864.0
23	7332.0
24	9856.0
25	13260.0
26	18554.0
27	25268.0
28	33826.0
29	45836.0
30	60796.0
31	81033.0
32	109572.0
33	150259.0
34	226757.0
35	439926.0
36	1451591.0
37	4008977.0
38	1847.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.471212944014407	15.543077041447223	20.907822814954947	40.077887199583415
2	19.952006443973275	46.470477000124525	17.004147549015304	16.573369006886892
3	22.20687906867554	31.03676971374555	29.09044060042411	17.665910617154797
4	35.05377623818496	22.780416359520874	19.92927526486443	22.236532137429734
5	22.89546020334402	25.637999562326712	24.28929043817761	27.17724979615166
6	22.771408763750077	27.302158059323574	24.406422108429613	25.52001106849674
7	23.280939240615172	25.808512749516844	25.632774819058955	25.27777319080902
8	23.156926330609974	25.446834364747044	26.823257672524882	24.5729816321181
9	23.22494005452591	27.702797792661706	25.569188189960578	23.503073962851808
10-14	23.825862703483466	25.711692828725553	25.33171748302841	25.130726984762575
15-19	22.91776409113852	25.71781603548356	26.35228038233847	25.012139491039452
20-24	23.280384104047336	24.881208485094177	26.157117073340146	25.68129033751834
25-29	23.19973375146877	25.835310270941275	27.118753434472413	23.84620254311754
30-34	22.93353413064104	26.862487696720027	26.397963349530546	23.80601482310839
35-39	23.311379683741244	26.13229517087176	27.186948417902556	23.369376727484436
40-44	22.988619000402156	26.929523413758112	26.55301543056721	23.52884215527252
45-49	23.133481261797513	26.9906317694888	26.93337768468387	22.942509284029825
50-54	23.283156999063305	26.72802809263313	26.952370948743535	23.036443959560028
55-59	23.018234711392303	27.348496780397515	26.684044763928895	22.949223744281287
60-64	23.027230090850345	26.725027207742635	27.426210079475773	22.82153262193125
65-69	23.487585782929983	27.12497629363661	27.170784235125222	22.216653688308185
70-74	22.913693911334278	27.250009723375264	26.806164077429145	23.030132287861314
75-79	23.378319277403605	27.466114225051914	26.479700912847832	22.675865584696652
80-84	22.792113617757064	27.371212928523057	26.56210407267105	23.274569381048828
85-89	23.259749303059177	27.230305303131935	26.71046538891958	22.79948000488931
90-94	23.080659782793205	26.725784984378215	26.953290065335402	23.24026516749318
95-99	23.143227162328866	26.273323991444798	27.031025533864977	23.552423312361363
100	24.060862955370922	25.753861770867275	26.079564350447676	24.105710923314124
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	63.549100143597826
2	36.52537545086017
3	39.872789685830334
4	57.29030837561469
5	50.07270999949568
6	48.29141983224682
7	48.5587124314242
8	47.72990796272808
9	46.72801401737772
10-14	48.95658968824604
15-19	47.92990358217797
20-24	48.96167444156568
25-29	47.04593629458631
30-34	46.739548953749434
35-39	46.68075641122568
40-44	46.51746115567468
45-49	46.07599054582733
50-54	46.31960095862333
55-59	45.967458455673594
60-64	45.8487627127816
65-69	45.70423947123817
70-74	45.94382619919559
75-79	46.05418486210026
80-84	46.06668299880589
85-89	46.059229307948485
90-94	46.32092495028639
95-99	46.695650474690225
100	48.16657387868505
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	184.0
1	181.0
2	180.0
3	182.0
4	183.5
5	192.0
6	196.5
7	200.5
8	222.0
9	242.0
10	258.5
11	290.5
12	328.5
13	355.5
14	383.5
15	664.0
16	869.5
17	894.5
18	1119.5
19	1926.5
20	2776.5
21	3247.0
22	4859.5
23	8959.5
24	17464.0
25	26142.0
26	33813.0
27	41525.0
28	48258.0
29	63146.0
30	85242.0
31	97999.0
32	109365.5
33	124450.0
34	146982.0
35	158993.5
36	151879.0
37	154523.0
38	176066.0
39	207147.5
40	240274.0
41	241125.5
42	221134.0
43	248956.0
44	278105.0
45	274935.5
46	252340.0
47	216380.0
48	210539.0
49	232911.0
50	256976.0
51	276411.0
52	240568.0
53	175884.5
54	158095.0
55	150932.5
56	120184.5
57	95956.0
58	120952.0
59	131092.0
60	120374.0
61	126934.0
62	112583.0
63	94251.5
64	75914.0
65	70581.5
66	90444.5
67	83946.0
68	54080.5
69	39899.0
70	34393.5
71	30368.5
72	26209.5
73	16814.0
74	11073.0
75	12525.0
76	11833.0
77	11275.5
78	9047.0
79	6781.0
80	5962.5
81	5579.5
82	3828.5
83	2678.0
84	2506.0
85	1526.5
86	2771.5
87	2004.5
88	505.5
89	360.5
90	272.5
91	230.0
92	154.5
93	111.0
94	96.0
95	87.5
96	76.0
97	65.5
98	65.5
99	57.0
100	50.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03848434459242149
2	0.010651536327912605
3	0.003659609867736344
4	0.0027967771011792137
5	0.0013388874351216058
6	0.003049694398306453
7	1.1901310170606767E-4
8	0.0015322945962812846
9	4.4629939697513144E-5
10-14	4.2844782902360935E-4
15-19	0.012993324965675693
20-24	0.012685159286084116
25-29	0.008579957255563623
30-34	0.009999972619122591
35-39	0.00622644398140972
40-44	0.01077764183113236
45-49	0.01023044547235071
50-54	0.01044309861731347
55-59	0.010017442699748167
60-64	0.03889740949004164
65-69	0.03047858316354786
70-74	0.019246892183083574
75-79	0.03979345501541018
80-84	0.04570682135748363
85-89	0.012460724278124483
90-94	0.002350654146574152
95-99	0.006300019801412913
100	0.012084659665971706
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
0-1	179.0
2-3	12.0
4-5	38.0
6-7	40.0
8-9	0.0
10-11	6.0
12-13	4.0
14-15	10.0
16-17	13.0
18-19	89.0
20-21	766.0
22-23	133.0
24-25	552.0
26-27	131.0
28-29	94.0
30-31	158.0
32-33	81.0
34-35	145.0
36-37	76.0
38-39	84.0
40-41	154.0
42-43	102.0
44-45	101.0
46-47	169.0
48-49	158.0
50-51	13772.0
52-53	454.0
54-55	13503.0
56-57	24743.0
58-59	54101.0
60-61	718.0
62-63	23221.0
64-65	14035.0
66-67	9850.0
68-69	12791.0
70-71	20956.0
72-73	29337.0
74-75	9451.0
76-77	8608.0
78-79	3744.0
80-81	8280.0
82-83	15230.0
84-85	8272.0
86-87	4805.0
88-89	8550.0
90-91	17592.0
92-93	399.0
94-95	7119.0
96-97	145248.0
98-99	0.0
100-101	6264140.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	98.3148706661228
#Duplication Level	Relative count
1	100.0
2	12.437543035717438
3	3.970762195660234
4	1.7223114814880207
5	0.8554176450855417
6	0.5217253129469094
7	0.30985716555730153
8	0.2780769434488603
9	0.1677289500167729
10++	7.696993237874962
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG	9994	0.14867125622599936	No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA	9919	0.14755555238199794	No Hit
GACAACTTGGCCACCGACAGCTGAAGGGCTTTTCACCTGTTGACACAATTGCCAGTCA	9814	0.145993567000396	No Hit
TGACTGGCAATTGTGTCAACAGGTGAAAAGCCCTTCAGCTGTCGGTGGCCAAGTTGTC	9795	0.1457109220265823	No Hit
CATGCACTATCCAGGCGCCTTCACCTACTCCCCGACGCCGGTCACCTCGGGCATCGGCATCGG	9368	0.13935884814140104	No Hit
CCGATGCCGATGCCCGAGGTGACCGGCGTCGGGGAGTAGGTGAAGGCGCCTGGATAGTGCATG	9101	0.13538694245675606	No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG	8760	0.13031420897936305	No Hit
GAGCTACCATCTGATCTGTCTGTCTTGACCACCCGGAGTCCCACTGTCCCCAGCCAGAATCCCAGTAGACTA	8753	0.1302100766205896	No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC	8738	0.1299869358517893	No Hit
TAGTCTACTGGGATTCTGGCTGGGGACAGTGGGACTCCGGGTGGTCAAGACAGACAGATCAGATGGTAGCTC	8649	0.128662967290241	No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT	8497	0.12640180749973148	No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT	8421	0.12527122760447673	No Hit
ATTCAGCTGCCAAGTAACTTAGCACACCCATTCTTATAAGATAATGGCAA	7991	0.1188745255655354	No Hit
TAATGAGCATGAAGTCACCACACGGAGGAAAATGTAAATGTGTAAACCTC	7957	0.11836873982292145	No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA	7595	0.11298360926920803	No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC	7557	0.11241831932158065	No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA	7378	0.10975550614723067	No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG	7371	0.1096513737884572	No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT	7229	0.10753897451048122	No Hit
GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG	7199	0.10709269297288065	No Hit
AGCCACCTCACCCACCCGGAATCTACATTTTCAATCAGTAAAAGTCACCA	7184	0.10686955220408037	No Hit
CATGTCATCTCAAAGGTCCCCAGGATTCGAACACCCAGTTATTCTCCAAC	7157	0.10646789882023987	No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA	6967	0.10364144908210302	No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC	6948	0.10335880410828932	No Hit
ACCTATGTTACACCATCTTCAGTGCCAGTCTTGGGCAAAATTGTGCAAGA	6776	0.10080012329271278	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	2641855	3.2269456	4.895105	75-79
AAAAA	2617955	3.040673	4.365095	2
CCCAG	1505905	2.8996425	5.428796	2
CCCCA	1322045	2.505746	6.088839	1
CCAGC	1251825	2.4104078	5.0971494	5
CCTCC	1222765	2.34104	5.6710687	6
TGGGG	1143535	2.33206	8.288691	3
GGGGG	996230	2.3094144	5.907885	4
CTGCC	1177185	2.2896373	5.5039673	7
GGAAG	1196775	2.1042671	5.098763	4
CCACC	1106305	2.0968418	5.95184	1
TGGCT	1127115	1.9904466	6.492927	3
CCCTG	1005085	1.9549006	5.244023	1
TGCAG	1101450	1.9256265	5.4602847	3
GCCCA	939680	1.8093681	5.7888975	2
GGCTC	909970	1.798064	7.8647113	1
TGGCA	1007905	1.762085	5.1416163	3
GCTCC	843365	1.6403539	7.627665	2
GATGG	888115	1.5773664	5.041971	1
TGCCC	810240	1.5759254	9.713757	3
TAAAA	1337875	1.5696329	5.889064	1
TGAGG	829700	1.4736165	5.003374	1
CATGG	837375	1.4639533	36.809944	1
ATGGA	939875	1.4538063	10.389134	2
GGCCC	630030	1.3929487	5.5849447	1
TGGGT	739155	1.3260928	5.7498965	3
ATGGG	746455	1.3257664	11.860283	2
ATGTG	836415	1.3068727	9.802917	2
ATGGC	730525	1.2771512	11.4350815	2
TGAGA	789200	1.2207409	5.0232034	1
ATGAA	877500	1.1821065	7.8245893	2
ATGCC	671630	1.1557962	9.312797	2
ATGTT	811710	1.1157334	6.235039	2
CATGA	705725	1.0745232	22.989094	1
CATGT	685375	1.0541042	27.77104	1
ATGCT	685205	1.0538429	8.114627	2
ATGAG	677385	1.0477846	5.469965	2
ATGGT	626415	0.97875416	6.335554	2
CATGC	560170	0.9639865	28.131937	1
ATGAC	606080	0.9228057	6.303243	2
ATGTA	629365	0.85642064	7.0857334	2
TAATG	596425	0.81159675	5.2849865	1
ATGCA	522010	0.79480237	9.579981	2
ATGTC	493235	0.75859374	5.151902	2
TAAGT	548785	0.7467698	6.1612115	1
>>END_MODULE