FastQCFastQC Report
Wed 20 Mar 2013
8016-7-7.h.sapiens.aligned.bam

Summary

[OK] Basic Statistics

Measure Value
Filename 8016-7-7.h.sapiens.aligned.bam
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 6722214
Filtered Sequences 0
Sequence length 1-100
%GC 46

[WARN] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG 9994 0.14867125622599936 No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA 9919 0.14755555238199794 No Hit
GACAACTTGGCCACCGACAGCTGAAGGGCTTTTCACCTGTTGACACAATTGCCAGTCA 9814 0.145993567000396 No Hit
TGACTGGCAATTGTGTCAACAGGTGAAAAGCCCTTCAGCTGTCGGTGGCCAAGTTGTC 9795 0.1457109220265823 No Hit
CATGCACTATCCAGGCGCCTTCACCTACTCCCCGACGCCGGTCACCTCGGGCATCGGCATCGG 9368 0.13935884814140104 No Hit
CCGATGCCGATGCCCGAGGTGACCGGCGTCGGGGAGTAGGTGAAGGCGCCTGGATAGTGCATG 9101 0.13538694245675606 No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG 8760 0.13031420897936305 No Hit
GAGCTACCATCTGATCTGTCTGTCTTGACCACCCGGAGTCCCACTGTCCCCAGCCAGAATCCCAGTAGACTA 8753 0.1302100766205896 No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC 8738 0.1299869358517893 No Hit
TAGTCTACTGGGATTCTGGCTGGGGACAGTGGGACTCCGGGTGGTCAAGACAGACAGATCAGATGGTAGCTC 8649 0.128662967290241 No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT 8497 0.12640180749973148 No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT 8421 0.12527122760447673 No Hit
ATTCAGCTGCCAAGTAACTTAGCACACCCATTCTTATAAGATAATGGCAA 7991 0.1188745255655354 No Hit
TAATGAGCATGAAGTCACCACACGGAGGAAAATGTAAATGTGTAAACCTC 7957 0.11836873982292145 No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA 7595 0.11298360926920803 No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC 7557 0.11241831932158065 No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA 7378 0.10975550614723067 No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG 7371 0.1096513737884572 No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT 7229 0.10753897451048122 No Hit
GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG 7199 0.10709269297288065 No Hit
AGCCACCTCACCCACCCGGAATCTACATTTTCAATCAGTAAAAGTCACCA 7184 0.10686955220408037 No Hit
CATGTCATCTCAAAGGTCCCCAGGATTCGAACACCCAGTTATTCTCCAAC 7157 0.10646789882023987 No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA 6967 0.10364144908210302 No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC 6948 0.10335880410828932 No Hit
ACCTATGTTACACCATCTTCAGTGCCAGTCTTGGGCAAAATTGTGCAAGA 6776 0.10080012329271278 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 2641855 3.2269456 4.895105 75-79
AAAAA 2617955 3.040673 4.365095 2
CCCAG 1505905 2.8996425 5.428796 2
CCCCA 1322045 2.505746 6.088839 1
CCAGC 1251825 2.4104078 5.0971494 5
CCTCC 1222765 2.34104 5.6710687 6
TGGGG 1143535 2.33206 8.288691 3
GGGGG 996230 2.3094144 5.907885 4
CTGCC 1177185 2.2896373 5.5039673 7
GGAAG 1196775 2.1042671 5.098763 4
CCACC 1106305 2.0968418 5.95184 1
TGGCT 1127115 1.9904466 6.492927 3
CCCTG 1005085 1.9549006 5.244023 1
TGCAG 1101450 1.9256265 5.4602847 3
GCCCA 939680 1.8093681 5.7888975 2
GGCTC 909970 1.798064 7.8647113 1
TGGCA 1007905 1.762085 5.1416163 3
GCTCC 843365 1.6403539 7.627665 2
GATGG 888115 1.5773664 5.041971 1
TGCCC 810240 1.5759254 9.713757 3
TAAAA 1337875 1.5696329 5.889064 1
TGAGG 829700 1.4736165 5.003374 1
CATGG 837375 1.4639533 36.809944 1
ATGGA 939875 1.4538063 10.389134 2
GGCCC 630030 1.3929487 5.5849447 1
TGGGT 739155 1.3260928 5.7498965 3
ATGGG 746455 1.3257664 11.860283 2
ATGTG 836415 1.3068727 9.802917 2
ATGGC 730525 1.2771512 11.4350815 2
TGAGA 789200 1.2207409 5.0232034 1
ATGAA 877500 1.1821065 7.8245893 2
ATGCC 671630 1.1557962 9.312797 2
ATGTT 811710 1.1157334 6.235039 2
CATGA 705725 1.0745232 22.989094 1
CATGT 685375 1.0541042 27.77104 1
ATGCT 685205 1.0538429 8.114627 2
ATGAG 677385 1.0477846 5.469965 2
ATGGT 626415 0.97875416 6.335554 2
CATGC 560170 0.9639865 28.131937 1
ATGAC 606080 0.9228057 6.303243 2
ATGTA 629365 0.85642064 7.0857334 2
TAATG 596425 0.81159675 5.2849865 1
ATGCA 522010 0.79480237 9.579981 2
ATGTC 493235 0.75859374 5.151902 2
TAAGT 548785 0.7467698 6.1612115 1
Produced by FastQC (version 0.10.0)