##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	8016-7-8.h.sapiens.aligned.bam	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	8250800	
Filtered Sequences	0	
Sequence length	1-100	
%GC	46	
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.815107262326077	24.0	24.0	32.0	24.0	32.0
2	31.002570134803296	31.0	31.0	32.0	30.0	32.0
3	32.68749795467883	34.0	33.0	34.0	30.0	34.0
4	34.25129900619814	35.0	35.0	35.0	29.0	37.0
5	35.37549575603022	36.0	36.0	36.0	34.0	36.0
6	35.55232773207022	36.0	36.0	37.0	34.0	37.0
7	35.90539214818826	36.0	35.0	37.0	33.0	38.0
8	36.24911698959174	37.0	36.0	37.0	34.0	38.0
9	36.48656397593882	37.0	37.0	38.0	35.0	38.0
10-14	36.75666813009617	37.6	36.8	38.0	34.4	38.2
15-19	37.256023832342336	38.0	37.0	38.8	34.6	39.2
20-24	36.881875372608405	37.8	36.6	38.2	35.0	38.6
25-29	37.00493146734986	38.0	36.8	38.6	34.2	39.0
30-34	37.00054768769983	37.8	36.8	38.6	35.4	39.2
35-39	36.908008832789115	38.0	37.2	38.4	33.6	39.2
40-44	36.78037783050761	38.0	36.8	38.2	33.6	39.2
45-49	36.644197166940785	37.2	37.0	38.0	33.8	39.2
50-54	36.89169916802256	38.2	37.0	38.2	34.0	39.0
55-59	36.941581074085114	38.0	37.2	38.4	33.0	38.8
60-64	36.43625948805593	37.4	36.4	38.0	33.4	38.4
65-69	36.52641931510225	37.8	36.4	38.4	33.8	38.8
70-74	36.5762901598964	37.6	36.8	38.6	34.2	38.6
75-79	36.23991829642914	37.2	36.6	37.8	34.2	38.6
80-84	36.485345493696784	37.8	36.4	38.0	33.4	38.8
85-89	36.40550052629969	38.0	36.2	38.6	33.0	39.2
90-94	36.19989516575928	37.6	36.4	38.4	33.2	39.6
95-99	35.8952115189267	37.2	36.2	38.0	32.4	38.6
100	34.88299827782511	37.0	35.0	37.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
1	1.0
2	0.0
3	1.0
4	0.0
5	44.0
6	219.0
7	398.0
8	524.0
9	893.0
10	828.0
11	1074.0
12	1414.0
13	1555.0
14	2083.0
15	2427.0
16	3010.0
17	3637.0
18	4596.0
19	4770.0
20	4714.0
21	5622.0
22	6895.0
23	9001.0
24	11858.0
25	16229.0
26	22180.0
27	30421.0
28	41526.0
29	55753.0
30	73732.0
31	99447.0
32	133106.0
33	183249.0
34	275169.0
35	533873.0
36	1769116.0
37	4949201.0
38	2234.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.349357472322318	15.755474880473713	21.072686508113094	39.822481139090876
2	19.785232309024202	46.732700919238106	17.149026056595314	16.333040715142374
3	22.056516664222315	31.405304770366204	29.23116828337359	17.307010282037886
4	34.77764179567963	23.03901848533666	20.10060520450909	22.08273451447462
5	22.657172206280283	25.909089531751395	24.597060151446744	26.83667811052158
6	22.55434548757339	27.53680858565798	24.71574122545837	25.193104701310265
7	23.081727465223192	26.050600753654034	25.85392104934628	25.01375073177649
8	22.92876283987714	25.691462844519357	26.99245888457622	24.387315431027286
9	23.01478765136268	27.943309861928995	25.77526236400009	23.266640122708235
10-14	23.63690483087658	25.92558376669627	25.538110929501862	24.899400472925286
15-19	22.69295152827573	25.999412277637017	26.56127117156351	24.74636502252374
20-24	23.03957314448187	25.07062350830729	26.429484570652544	25.4603187765583
25-29	23.00738819246208	26.039363923949495	27.281321516325175	23.671926367263254
30-34	22.764717905101122	27.06025595968683	26.60149168353641	23.573534451675638
35-39	23.16164733812669	26.282820225613936	27.37032766703635	23.185204769223024
40-44	22.775956903854066	27.07585104355304	26.767789297426315	23.38040275516658
45-49	22.997625019014595	27.147848750935204	27.12678232713794	22.72774390291226
50-54	23.08825179185666	26.949011091799335	27.09576208852499	22.866975027819016
55-59	22.841824365086662	27.563168569902608	26.84827465843408	22.746732406576655
60-64	22.824346671535487	26.965349751708967	27.577237129909893	22.633066446845653
65-69	23.277212318691447	27.326410673085462	27.359576239500978	22.036800768722113
70-74	22.75708157939468	27.405817243758314	26.998247727795388	22.838853449051623
75-79	23.17055733046111	27.599995040029	26.72768152016169	22.501766109348196
80-84	22.63307232348217	27.510183496573294	26.773368997489307	23.083375182455235
85-89	23.05280069890939	27.409392795368852	26.869486000677867	22.668320505043887
90-94	22.864260593386703	26.908734225512028	27.150576703541052	23.076428477560217
95-99	22.940203842761917	26.458866625190673	27.21557546140328	23.385354070644127
100	23.82002062187164	25.952979839605927	26.388152403813457	23.838847134708978
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	63.1718386114132
2	36.11827302416658
3	39.3635269462602
4	56.86037631015425
5	49.49385031680186
6	47.74745018888365
7	48.095478196999686
8	47.31607827090442
9	46.28142777407091
10-14	48.53630530380187
15-19	47.43931655079947
20-24	48.49989192104017
25-29	46.679314559725334
30-34	46.33825235677676
35-39	46.34685210734972
40-44	46.156359659020644
45-49	45.72536892192685
50-54	45.95522681967567
55-59	45.58855677166331
60-64	45.45741311838114
65-69	45.314013087413564
70-74	45.5959350284463
75-79	45.672323439809304
80-84	45.7164475059374
85-89	45.721121203953274
90-94	45.940689070946924
95-99	46.325557913406044
100	47.65886775658061
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	264.0
1	264.5
2	267.0
3	270.5
4	276.5
5	282.0
6	288.0
7	305.0
8	323.0
9	348.0
10	378.5
11	402.5
12	430.0
13	473.5
14	539.0
15	834.0
16	1016.0
17	1056.0
18	1363.5
19	2451.0
20	3673.0
21	4413.0
22	6603.5
23	11813.5
24	22087.0
25	33429.0
26	43280.5
27	52805.0
28	62750.0
29	82677.5
30	112234.0
31	128004.5
32	139761.5
33	159547.0
34	188267.5
35	202900.5
36	192686.0
37	196568.5
38	225928.5
39	263570.0
40	302383.0
41	301224.0
42	275084.0
43	312282.0
44	350896.5
45	342926.0
46	309698.5
47	265764.5
48	262245.5
49	283410.5
50	307652.5
51	334607.0
52	289331.5
53	211732.0
54	188452.0
55	177404.0
56	142497.0
57	115327.0
58	144950.0
59	153907.0
60	141704.0
61	149012.5
62	130605.0
63	108457.5
64	88136.0
65	83297.0
66	105701.0
67	98502.5
68	62249.0
69	45596.5
70	40014.0
71	33692.0
72	29420.0
73	19124.0
74	12978.0
75	14552.5
76	13535.5
77	12482.0
78	9179.5
79	7585.5
80	7130.0
81	6371.5
82	4272.0
83	2939.5
84	2764.0
85	1657.0
86	2869.5
87	2034.0
88	507.0
89	386.5
90	274.5
91	204.5
92	128.0
93	92.0
94	80.5
95	63.0
96	48.0
97	44.0
98	45.0
99	40.5
100	33.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03877199786687351
2	0.010690209368114087
3	0.002763456152798763
4	0.0032603934434336282
5	0.0012726455148941945
6	0.0024604509776353493
7	9.696407892972989E-5
8	0.0011878112626082227
9	4.848209235135603E-5
10-14	3.2240614860038765E-4
15-19	0.011572732673438696
20-24	0.011573959309168939
25-29	0.008872105196835013
30-34	0.010143129179357804
35-39	0.006641910454970866
40-44	0.010546356048472207
45-49	0.010042286489676198
50-54	0.010226266220655717
55-59	0.010004435177441056
60-64	0.03784475969021784
65-69	0.029097679542586366
70-74	0.017757037491341724
75-79	0.03895278775293981
80-84	0.04472921537738828
85-89	0.012167537546665863
90-94	0.0023803126953533048
95-99	0.0070516217804280305
100	0.012948879283616934
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
0-1	260.0
2-3	2.0
4-5	18.0
6-7	51.0
8-9	0.0
10-11	6.0
12-13	4.0
14-15	4.0
16-17	33.0
18-19	80.0
20-21	1031.0
22-23	186.0
24-25	745.0
26-27	130.0
28-29	120.0
30-31	240.0
32-33	76.0
34-35	136.0
36-37	91.0
38-39	69.0
40-41	141.0
42-43	82.0
44-45	104.0
46-47	142.0
48-49	134.0
50-51	17798.0
52-53	696.0
54-55	18583.0
56-57	31953.0
58-59	66470.0
60-61	1137.0
62-63	29071.0
64-65	17309.0
66-67	13206.0
68-69	15878.0
70-71	28210.0
72-73	35997.0
74-75	11405.0
76-77	11199.0
78-79	4785.0
80-81	11344.0
82-83	19622.0
84-85	10007.0
86-87	5579.0
88-89	9871.0
90-91	22763.0
92-93	457.0
94-95	8695.0
96-97	178540.0
98-99	0.0
100-101	7676340.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	98.40592427400979
#Duplication Level	Relative count
1	100.0
2	13.281986557586448
3	4.355859006721206
4	1.8901493263586875
5	1.0355524467017427
6	0.5702381314436908
7	0.3535476414950883
8	0.25926827042973144
9	0.20300477479395396
10++	6.89531948541711
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CATGCACTATCCAGGCGCCTTCACCTACTCCCCGACGCCGGTCACCTCGGGCATCGGCATCGG	11851	0.14363455664905223	No Hit
GACAACTTGGCCACCGACAGCTGAAGGGCTTTTCACCTGTTGACACAATTGCCAGTCA	11836	0.14345275609637853	No Hit
TGACTGGCAATTGTGTCAACAGGTGAAAAGCCCTTCAGCTGTCGGTGGCCAAGTTGTC	11758	0.1425073932224754	No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG	11538	0.13984098511659476	No Hit
CCGATGCCGATGCCCGAGGTGACCGGCGTCGGGGAGTAGGTGAAGGCGCCTGGATAGTGCATG	11450	0.1387744218742425	No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA	11410	0.138289620400446	No Hit
GAGCTACCATCTGATCTGTCTGTCTTGACCACCCGGAGTCCCACTGTCCCCAGCCAGAATCCCAGTAGACTA	10886	0.13193872109371213	No Hit
TAGTCTACTGGGATTCTGGCTGGGGACAGTGGGACTCCGGGTGGTCAAGACAGACAGATCAGATGGTAGCTC	10701	0.12969651427740342	No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC	10125	0.12271537305473408	No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG	10102	0.12243661220730112	No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT	9678	0.11729771658505841	No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT	9659	0.11706743588500508	No Hit
ATTCAGCTGCCAAGTAACTTAGCACACCCATTCTTATAAGATAATGGCAA	9253	0.11214670092597082	No Hit
TAATGAGCATGAAGTCACCACACGGAGGAAAATGTAAATGTGTAAACCTC	9197	0.11146797886265575	No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA	8999	0.10906821156736317	No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG	8973	0.10875309060939546	No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA	8745	0.10598972220875551	No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC	8708	0.10554128084549377	No Hit
GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG	8527	0.10334755417656469	No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA	8516	0.10321423377127065	No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT	8508	0.10311727347651137	No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC	8488	0.10287487273961313	No Hit
ACCTATGTTACACCATCTTCAGTGCCAGTCTTGGGCAAAATTGTGCAAGA	8367	0.10140834828137876	No Hit
TAAAACTGCATCAAGTCATGGGGCATGTGGAAGGTAGGGAGGCAAGATGA	8284	0.10040238522325108	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	3306405	3.1727278	4.776519	75-79
AAAAA	3291015	3.0051918	4.3581595	2
CCCAG	1796275	2.8945365	5.348742	2
CCCCA	1581130	2.5082693	6.0659814	1
CCAGC	1504395	2.424198	5.172347	5
CCTCC	1468400	2.3526528	5.604707	6
TGGGG	1374205	2.3440309	8.151389	3
GGGGG	1190025	2.3438332	5.7783737	4
CTGCC	1404415	2.2856436	5.5608697	7
CCACC	1333830	2.1159582	5.903433	1
GGAAG	1452345	2.103338	5.020593	4
TGGCT	1368410	1.9900762	6.251745	3
CCCTG	1203880	1.959279	5.227167	1
TGCAG	1327710	1.9118322	5.6273212	3
GCCCA	1125525	1.8136828	5.614962	2
GGCTC	1083195	1.7906854	7.3626328	1
TGGCA	1235490	1.7790406	5.104687	3
GCTCC	1002940	1.6322551	7.0667596	2
TGCCC	962470	1.5663913	9.576345	3
TAAAA	1677330	1.5469198	5.920756	1
TGAGG	1011820	1.479958	5.042492	1
CATGG	1021135	1.4703807	36.164043	1
ATGGA	1157425	1.4516885	10.046238	2
GGCCC	746105	1.4020767	5.550292	1
TGGCC	824355	1.3627837	5.163738	3
TGGGT	897415	1.3257035	5.609238	3
ATGGG	898485	1.3141867	11.603185	2
ATGTG	1025655	1.299238	9.604631	2
ATGGC	891535	1.2837636	11.115032	2
ATGAA	1099660	1.1826966	7.850102	2
ATGCC	813715	1.1535048	9.081499	2
TGAGC	784950	1.1302866	5.0294313	1
ATGTT	1014420	1.1128753	6.23479	2
CATGA	873690	1.0787935	23.177553	1
ATGCT	854860	1.066063	8.10213	2
CATGT	846415	1.0555315	27.55346	1
ATGAG	833575	1.0455029	5.3744917	2
ATGGT	778065	0.9856061	6.269948	2
CATGC	688955	0.9766476	28.488892	1
ATGAC	750860	0.9271285	6.2317	2
ATGTA	794520	0.8630314	7.0023484	2
TAATG	751125	0.81589454	5.0900874	1
ATGCA	654045	0.80758566	9.71299	2
TAAGT	687815	0.7471253	6.1564207	1
>>END_MODULE