##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	8016-7-9.h.sapiens.aligned.bam	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	9230846	
Filtered Sequences	0	
Sequence length	1-100	
%GC	45	
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.795767581866276	24.0	24.0	32.0	24.0	32.0
2	30.99670468533358	31.0	31.0	32.0	30.0	32.0
3	32.678392554841224	34.0	33.0	34.0	30.0	34.0
4	34.23712069644871	35.0	35.0	35.0	29.0	37.0
5	35.3681894998473	36.0	36.0	36.0	34.0	36.0
6	35.54788975698065	36.0	36.0	37.0	34.0	37.0
7	35.90534959049064	36.0	35.0	37.0	33.0	38.0
8	36.246365316697414	37.0	36.0	37.0	34.0	38.0
9	36.48319584967307	37.0	37.0	38.0	35.0	38.0
10-14	36.7570450932596	37.6	36.8	38.0	34.4	38.2
15-19	37.260746247916025	38.0	37.0	38.8	34.6	39.2
20-24	36.886853619297284	37.8	36.6	38.2	35.2	38.6
25-29	37.01173897500339	38.0	36.8	38.6	34.2	39.0
30-34	37.012697283991386	37.8	36.8	38.8	35.4	39.2
35-39	36.92519687925922	38.0	37.2	38.6	33.6	39.2
40-44	36.79880872122022	38.0	36.8	38.2	33.6	39.2
45-49	36.656725594680566	37.4	37.0	38.4	33.8	39.2
50-54	36.903023226887925	38.2	37.2	38.2	34.0	39.0
55-59	36.955500835834734	38.0	37.2	38.4	33.0	38.8
60-64	36.44520511146969	37.4	36.4	38.0	33.4	38.4
65-69	36.537986087990724	37.8	36.4	38.4	34.0	38.8
70-74	36.58904278668699	37.6	36.8	38.6	34.2	38.6
75-79	36.2511865483155	37.2	36.8	37.8	34.2	38.6
80-84	36.49952549255177	37.8	36.4	38.0	33.6	38.8
85-89	36.42770118022189	38.0	36.2	38.6	33.0	39.2
90-94	36.22396534165148	37.6	36.4	38.4	33.2	39.6
95-99	35.91639684126115	37.2	36.4	38.0	32.4	38.6
100	34.91481165124514	37.0	35.0	37.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
1	1.0
2	0.0
3	10.0
4	0.0
5	43.0
6	236.0
7	370.0
8	527.0
9	1189.0
10	1008.0
11	1344.0
12	1626.0
13	2024.0
14	2390.0
15	2807.0
16	3425.0
17	3903.0
18	4990.0
19	5378.0
20	5148.0
21	6380.0
22	7831.0
23	9704.0
24	13396.0
25	18169.0
26	24663.0
27	34246.0
28	46664.0
29	61934.0
30	82139.0
31	110718.0
32	147487.0
33	203356.0
34	305122.0
35	584990.0
36	1933736.0
37	5600821.0
38	3071.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.179793409474154	16.040131134663387	21.062342938018617	39.71773251784384
2	19.264523934409816	47.70413882032455	17.03557415463614	15.995763090629488
3	21.632439960117324	31.9313834245948	29.78202880952296	16.654147805764914
4	34.78651510586407	23.19652103104942	20.275173885067872	21.741789978018637
5	22.295535679558977	26.32654076079803	25.168953012975088	26.20897054666791
6	22.244984720632313	27.8040396988373	25.176179137503908	24.774796443026474
7	22.777107100384463	26.369177696550157	26.300707571571596	24.553007631493788
8	22.529676470996105	26.04667197098917	27.43412716159809	23.989524396416638
9	22.622543607980063	28.18382185812691	26.191580214133747	23.00205431975928
10-14	23.352297327673284	26.252459355548634	25.873456095013363	24.52178722176472
15-19	22.4082350820532	26.279727682223868	26.9325217375174	24.379515498205535
20-24	22.790873248943054	25.21108929281075	26.81495939844918	25.183078059797015
25-29	22.73772085171956	26.257844475382246	27.607873586776265	23.39656108612193
30-34	22.500495474421285	27.341840370291237	26.83553195072469	23.322132204562795
35-39	22.890713412957126	26.495878953660533	27.737410385081862	22.87599724830048
40-44	22.454139171370368	27.368782546898867	27.075499386377626	23.101578895353132
45-49	22.70179769915891	27.520277804496253	27.41344509611508	22.364479400229758
50-54	22.80966779832982	27.26050666374087	27.38513390329397	22.544691634635345
55-59	22.553271073162094	27.922038135666384	27.171651928175805	22.35303886299572
60-64	22.51478243625126	27.322761150865134	27.859274071380963	22.303182341502648
65-69	22.990029445718452	27.64216321362604	27.63088121882582	21.736926121829683
70-74	22.432965372821513	27.74058115135253	27.263548350392902	22.56290512543306
75-79	22.90290991324601	27.86427753723742	27.044690687220363	22.18812186229621
80-84	22.332684723765677	27.833520400091693	27.08660227779131	22.74719259835131
85-89	22.713796908174025	27.70502035933108	27.125601290169726	22.45558144232517
90-94	22.502437550622155	27.249967173646557	27.494425293819923	22.753169981911373
95-99	22.63989729068813	26.717216876071525	27.576132828046756	23.066753005193586
100	23.362568037781664	26.3804887683201	26.639302456621376	23.61764073727686
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	62.897525927318
2	35.260287025039304
3	38.28658776588224
4	56.5283050838827
5	48.50450622622689
6	47.019781163658784
7	47.33011473187825
8	46.51920086741274
9	45.624597927739345
10-14	47.874084549438
15-19	46.78775058025874
20-24	47.97395130874007
25-29	46.134281937841486
30-34	45.82262767898408
35-39	45.766710661257605
40-44	45.5557180667235
45-49	45.06627709938867
50-54	45.35435943296516
55-59	44.90630993615781
60-64	44.81796477775391
65-69	44.72695556754814
70-74	44.99587049825457
75-79	45.09103177554222
80-84	45.07987732211699
85-89	45.169378350499194
90-94	45.255607532533524
95-99	45.706650295881715
100	46.98020877505852
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	470.0
1	470.5
2	469.5
3	470.5
4	475.5
5	486.0
6	497.0
7	505.5
8	522.5
9	566.5
10	615.0
11	654.0
12	699.0
13	755.5
14	822.5
15	1137.0
16	1388.5
17	1453.0
18	1868.0
19	3177.5
20	4570.5
21	5373.5
22	8216.5
23	14864.5
24	28074.0
25	41799.5
26	53491.0
27	66443.5
28	78388.5
29	101156.5
30	136276.5
31	155571.0
32	171411.0
33	193657.5
34	224998.5
35	242484.0
36	227582.0
37	230923.5
38	266583.5
39	309850.5
40	354235.0
41	348867.5
42	317544.5
43	358459.5
44	397834.0
45	386085.0
46	341918.5
47	290866.5
48	291026.5
49	315590.0
50	342015.5
51	366292.5
52	308146.0
53	224150.0
54	200334.5
55	186646.0
56	148713.0
57	120892.0
58	152621.0
59	159604.0
60	145446.0
61	154577.5
62	136922.5
63	111972.5
64	89452.5
65	83931.5
66	106644.5
67	98606.0
68	63304.5
69	46743.0
70	41885.0
71	36804.0
72	28910.0
73	17363.0
74	12594.5
75	14854.5
76	13812.5
77	12614.5
78	9356.0
79	7422.0
80	6941.5
81	5906.0
82	3890.0
83	2943.0
84	2829.5
85	1660.5
86	2691.5
87	1875.5
88	565.0
89	439.0
90	279.0
91	228.5
92	153.5
93	123.0
94	111.0
95	79.5
96	67.5
97	64.0
98	59.0
99	51.0
100	45.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0387505110582497
2	0.010931296638122513
3	0.0032609729573254563
4	0.003033464202034783
5	0.0014300632445469902
6	0.0026109561570625555
7	1.19172543859288E-4
8	0.0010942212227131292
9	3.2501620476627596E-5
10-14	3.5751830553310714E-4
15-19	0.011923825239407682
20-24	0.011692916027982302
25-29	0.008643476012457615
30-34	0.009937764236697995
35-39	0.006261943942791713
40-44	0.01028938823116811
45-49	0.009973179711539487
50-54	0.010252127001604069
55-59	0.009858715892760743
60-64	0.038279205338763035
65-69	0.029444577735390418
70-74	0.018091719647039175
75-79	0.039146007681145734
80-84	0.04496172576040898
85-89	0.01223760374309493
90-94	0.0025857372859671244
95-99	0.006593489479360785
100	0.01241807583947761
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
0-1	468.0
2-3	7.0
4-5	36.0
6-7	26.0
8-9	0.0
10-11	16.0
12-13	2.0
14-15	14.0
16-17	16.0
18-19	80.0
20-21	839.0
22-23	171.0
24-25	1345.0
26-27	141.0
28-29	104.0
30-31	164.0
32-33	115.0
34-35	113.0
36-37	50.0
38-39	49.0
40-41	136.0
42-43	70.0
44-45	90.0
46-47	105.0
48-49	105.0
50-51	17265.0
52-53	583.0
54-55	22028.0
56-57	33009.0
58-59	66577.0
60-61	1277.0
62-63	29866.0
64-65	18415.0
66-67	13878.0
68-69	16279.0
70-71	28939.0
72-73	39850.0
74-75	12884.0
76-77	12162.0
78-79	4538.0
80-81	12663.0
82-83	22075.0
84-85	10591.0
86-87	5797.0
88-89	9176.0
90-91	25634.0
92-93	583.0
94-95	9564.0
96-97	204532.0
98-99	0.0
100-101	8608419.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	98.45509234638702
#Duplication Level	Relative count
1	100.0
2	13.06167956916863
3	4.556546617300572
4	2.088241893862897
5	1.1268652530012342
6	0.6605520026926961
7	0.41161786155054414
8	0.30082463816896665
9	0.2159766633007966
10++	6.526281835521149
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG	13421	0.14539295748190362	No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA	13271	0.14376797099637453	No Hit
CATGCACTATCCAGGCGCCTTCACCTACTCCCCGACGCCGGTCACCTCGGGCATCGGCATCGG	12012	0.1301289177611673	No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC	11711	0.1268681115468723	No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG	11662	0.12633728262826616	No Hit
CCGATGCCGATGCCCGAGGTGACCGGCGTCGGGGAGTAGGTGAAGGCGCCTGGATAGTGCATG	11582	0.1254706231693173	No Hit
GAGCTACCATCTGATCTGTCTGTCTTGACCACCCGGAGTCCCACTGTCCCCAGCCAGAATCCCAGTAGACTA	11462	0.12417063398089405	No Hit
GACAACTTGGCCACCGACAGCTGAAGGGCTTTTCACCTGTTGACACAATTGCCAGTCA	11455	0.12409480127823604	No Hit
TGACTGGCAATTGTGTCAACAGGTGAAAAGCCCTTCAGCTGTCGGTGGCCAAGTTGTC	11438	0.12391063614320941	No Hit
TAGTCTACTGGGATTCTGGCTGGGGACAGTGGGACTCCGGGTGGTCAAGACAGACAGATCAGATGGTAGCTC	11284	0.1222423166847329	No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT	10906	0.11814735074119967	No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT	10849	0.11752985587669862	No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC	10784	0.11682569506630269	No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA	10775	0.11672819587717097	No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA	10208	0.1105857469618711	No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG	10179	0.11027158290800214	No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA	10012	0.10846243128744645	No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC	10011	0.1084515980442096	No Hit
CATGGAATATATATTCTCCTGGCTGGTGCTTATGATTGCAAAACTTGGAT	9936	0.10763910480144506	No Hit
ATTCAGCTGCCAAGTAACTTAGCACACCCATTCTTATAAGATAATGGCAA	9928	0.10755243885555019	No Hit
TAATGAGCATGAAGTCACCACACGGAGGAAAATGTAAATGTGTAAACCTC	9907	0.1073249407475761	No Hit
CACACTGTCATTAGTTGCAGCATCTCCTGTAGCAATTGGGCTAAATCTGT	9904	0.10729244101786554	No Hit
ATCCTTCGAGGGCTTTGTCTGACAACTGTCTTATCTTCTTTTGTAAGTGG	9283	0.10056499696777521	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	3940115	3.1828754	4.8183336	75-79
AAAAA	3923060	3.0239356	4.3675714	2
CCCAG	1934560	2.9060853	5.190659	2
CCCCA	1700795	2.5168054	6.045392	1
CCAGC	1603975	2.4094822	5.225475	5
GGGGG	1267445	2.3800256	5.2077036	4
CCTCC	1573510	2.3503892	5.716723	6
TGGGG	1481235	2.3496766	7.328133	3
CTGCC	1528645	2.3179572	5.587548	7
CCACC	1451325	2.1476443	6.002725	1
TGGCT	1522485	2.009742	6.4317846	3
TGCAG	1453905	1.9013004	6.117821	3
GGCTC	1179305	1.8153207	6.5730896	1
TGGCA	1359150	1.7773875	5.236168	3
GCTCC	1084485	1.644456	6.226146	2
GATGG	1184555	1.572528	5.109635	1
TGCCC	1030805	1.5630587	9.4135475	3
TAAAA	1971415	1.5339043	5.76929	1
CATGG	1116810	1.4604745	38.046883	1
ATGGA	1311650	1.4572035	10.540634	2
AGACA	1242600	1.3472012	5.014646	2
TGGCC	869095	1.33781	5.14678	3
TGGGT	978445	1.3111495	5.830887	3
ATGTG	1159140	1.2999022	9.776243	2
ATGGG	976580	1.2964358	11.882715	2
ATGGC	975965	1.276289	11.385974	2
ATGAA	1275160	1.1855667	7.990884	2
ATGCC	898035	1.1568567	8.9615555	2
TGAGC	868230	1.1354016	5.1916394	1
ATGTT	1154195	1.0934166	6.596095	2
CATGA	977100	1.0693325	24.295666	1
ATGCT	965470	1.0665597	7.8415856	2
CATGT	956925	1.0571198	29.323277	1
ATGAG	925025	1.0276748	5.573728	2
ATGGT	885140	0.99262875	6.649436	2
CATGC	748230	0.9638764	29.084887	1
ATGAC	864070	0.9456332	6.58165	2
ATGTA	930730	0.8734892	7.4471283	2
ATGCA	741275	0.811247	10.055528	2
ATGTC	689875	0.76210845	5.131507	2
TAAGT	799800	0.75061154	5.860905	1
>>END_MODULE