FastQCFastQC Report
Wed 20 Mar 2013
8016-7-9.h.sapiens.aligned.bam

Summary

[OK] Basic Statistics

Measure Value
Filename 8016-7-9.h.sapiens.aligned.bam
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 9230846
Filtered Sequences 0
Sequence length 1-100
%GC 45

[WARN] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG 13421 0.14539295748190362 No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA 13271 0.14376797099637453 No Hit
CATGCACTATCCAGGCGCCTTCACCTACTCCCCGACGCCGGTCACCTCGGGCATCGGCATCGG 12012 0.1301289177611673 No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC 11711 0.1268681115468723 No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG 11662 0.12633728262826616 No Hit
CCGATGCCGATGCCCGAGGTGACCGGCGTCGGGGAGTAGGTGAAGGCGCCTGGATAGTGCATG 11582 0.1254706231693173 No Hit
GAGCTACCATCTGATCTGTCTGTCTTGACCACCCGGAGTCCCACTGTCCCCAGCCAGAATCCCAGTAGACTA 11462 0.12417063398089405 No Hit
GACAACTTGGCCACCGACAGCTGAAGGGCTTTTCACCTGTTGACACAATTGCCAGTCA 11455 0.12409480127823604 No Hit
TGACTGGCAATTGTGTCAACAGGTGAAAAGCCCTTCAGCTGTCGGTGGCCAAGTTGTC 11438 0.12391063614320941 No Hit
TAGTCTACTGGGATTCTGGCTGGGGACAGTGGGACTCCGGGTGGTCAAGACAGACAGATCAGATGGTAGCTC 11284 0.1222423166847329 No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT 10906 0.11814735074119967 No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT 10849 0.11752985587669862 No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC 10784 0.11682569506630269 No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA 10775 0.11672819587717097 No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA 10208 0.1105857469618711 No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG 10179 0.11027158290800214 No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA 10012 0.10846243128744645 No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC 10011 0.1084515980442096 No Hit
CATGGAATATATATTCTCCTGGCTGGTGCTTATGATTGCAAAACTTGGAT 9936 0.10763910480144506 No Hit
ATTCAGCTGCCAAGTAACTTAGCACACCCATTCTTATAAGATAATGGCAA 9928 0.10755243885555019 No Hit
TAATGAGCATGAAGTCACCACACGGAGGAAAATGTAAATGTGTAAACCTC 9907 0.1073249407475761 No Hit
CACACTGTCATTAGTTGCAGCATCTCCTGTAGCAATTGGGCTAAATCTGT 9904 0.10729244101786554 No Hit
ATCCTTCGAGGGCTTTGTCTGACAACTGTCTTATCTTCTTTTGTAAGTGG 9283 0.10056499696777521 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 3940115 3.1828754 4.8183336 75-79
AAAAA 3923060 3.0239356 4.3675714 2
CCCAG 1934560 2.9060853 5.190659 2
CCCCA 1700795 2.5168054 6.045392 1
CCAGC 1603975 2.4094822 5.225475 5
GGGGG 1267445 2.3800256 5.2077036 4
CCTCC 1573510 2.3503892 5.716723 6
TGGGG 1481235 2.3496766 7.328133 3
CTGCC 1528645 2.3179572 5.587548 7
CCACC 1451325 2.1476443 6.002725 1
TGGCT 1522485 2.009742 6.4317846 3
TGCAG 1453905 1.9013004 6.117821 3
GGCTC 1179305 1.8153207 6.5730896 1
TGGCA 1359150 1.7773875 5.236168 3
GCTCC 1084485 1.644456 6.226146 2
GATGG 1184555 1.572528 5.109635 1
TGCCC 1030805 1.5630587 9.4135475 3
TAAAA 1971415 1.5339043 5.76929 1
CATGG 1116810 1.4604745 38.046883 1
ATGGA 1311650 1.4572035 10.540634 2
AGACA 1242600 1.3472012 5.014646 2
TGGCC 869095 1.33781 5.14678 3
TGGGT 978445 1.3111495 5.830887 3
ATGTG 1159140 1.2999022 9.776243 2
ATGGG 976580 1.2964358 11.882715 2
ATGGC 975965 1.276289 11.385974 2
ATGAA 1275160 1.1855667 7.990884 2
ATGCC 898035 1.1568567 8.9615555 2
TGAGC 868230 1.1354016 5.1916394 1
ATGTT 1154195 1.0934166 6.596095 2
CATGA 977100 1.0693325 24.295666 1
ATGCT 965470 1.0665597 7.8415856 2
CATGT 956925 1.0571198 29.323277 1
ATGAG 925025 1.0276748 5.573728 2
ATGGT 885140 0.99262875 6.649436 2
CATGC 748230 0.9638764 29.084887 1
ATGAC 864070 0.9456332 6.58165 2
ATGTA 930730 0.8734892 7.4471283 2
ATGCA 741275 0.811247 10.055528 2
ATGTC 689875 0.76210845 5.131507 2
TAAGT 799800 0.75061154 5.860905 1
Produced by FastQC (version 0.10.0)