FastQCFastQC Report
Wed 20 Mar 2013
8016-8-15.h.sapiens.aligned.bam

Summary

[OK] Basic Statistics

Measure Value
Filename 8016-8-15.h.sapiens.aligned.bam
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 4362356
Filtered Sequences 0
Sequence length 1-100
%GC 44

[WARN] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
TGAGACCGCACCAGCGACACA 13779 0.315861429007628 No Hit
TGTGTCGCTGGTGCGGTCTCA 13573 0.3111392100965625 No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC 9205 0.21100983046775643 No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG 9144 0.2096115035086545 No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA 8969 0.20559990977352607 No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC 8869 0.2033075704963098 No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT 8281 0.1898286155462782 No Hit
GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG 8235 0.18877413947875873 No Hit
CATGGGGTTGTGCCAATTAATTACCAAACATTGAGCCTGCAGGCTTTGAG 8187 0.1876738166256949 No Hit
TAAAGAAACCCACAGAAACACACACACATACAATTAGCAAATGAATTCAA 8158 0.1870090382353022 No Hit
AAAAATAATAAACCGTTCATTTCTCAGGATGTGGTCATAGAATAAAGTTA 8124 0.18622964288104868 No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA 8016 0.18375391646165512 No Hit
AAGACAGGGGAAAGGAAGAAAAGGGAGTGAAGAGGGAATGGGAGAGGGAA 7997 0.18331837199898401 No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG 7897 0.1810260327217678 No Hit
GATACAACATTCAAGACAGAGGAACTGGATGCCAAGCCAGACACAGACCC 7645 0.17524933774318283 No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG 7633 0.17497425702991687 No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA 7592 0.1740343979262582 No Hit
CATGTATTTTGAACCGGCACCCCTGTTACCACAGAGTGTGGGAGGAACTG 7523 0.17245268382497897 No Hit
CACTCAGTATGAGGAGCCGGCTGTCATCACATAGGGACAGGGCTCTCCCT 7471 0.17126066740082652 No Hit
* 7353 0.16855570705371134 No Hit
CATGCCCTCCAGCGGCCCCGGGGACACCAGCAGCTCTGCTGCGGAGCGGG 7286 0.16701983973797643 No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT 6683 0.1531970338963624 No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT 6620 0.15175286015171618 No Hit
CATGTAGACTTACCTTTCCTCATAGAGCTATCCTGGTTAATAACAGGCCA 6608 0.15147777943845023 No Hit
ACACTTACACCAAGGAATCTCCCAGGCATTATCAAAGCTACAGGAGACAA 6495 0.14888743605519586 No Hit
CATGTAATTCAGGCAGTTATGGGAGCCCTAGAGGGCCTGAGAGTTGCTATTGGAC 6479 0.14852066177084125 No Hit
GTCCAATAGCAACTCTCAGGCCCTCTAGGGCTCCCATAACTGCCTGAATTACATG 6433 0.1474661857033218 No Hit
AGCAACAAGAACAATACAAACCTCATTTCCTTTAGTCCTTCTGCCTCTAT 5833 0.13371215004002424 No Hit
TAACTGTGGAAAAACTAAGTTGTCACCAGCGGTACCTCATAGCATAACTT 5790 0.13272644415082124 No Hit
CATGGAATATATATTCTCCTGGCTGGTGCTTATGATTGCAAAACTTGGAT 5642 0.1293337820205412 No Hit
CACACTGTCATTAGTTGCAGCATCTCCTGTAGCAATTGGGCTAAATCTGT 5615 0.1287148504156928 No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC 5577 0.12784376149035062 No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA 5577 0.12784376149035062 No Hit
CATGGGCTCGGCCACGCGCTACCACACCTACCTGCCGCCGCCCTACCCCG 5568 0.12763745095540116 No Hit
ACAGATTTTGGGGTTGTGTTGTCACCCAAGAGATTGTTGTTTGCCATACT 5544 0.12708728952886927 No Hit
GAGAACTGGTAGGAGCCGGCCGAGGCGCCGTAGTACAGGTGGTAGGAGGG 5477 0.12555142221313437 No Hit
ATCCTTCACAAAGTCTTTGAGATATATTTTTATCAAATATTTAGCATGGATCCCGGTACACTTTCAATACTTA 5384 0.12341954668532325 No Hit
TAAGTATTGAAAGTGTACCGGGATCCATGCTAAATATTTGATAAAAATATATCTCAAAGACTTTGTGAAGGAT 5340 0.12241091740334809 No Hit
CATGGCTCCACTCTCCACCGTCTCCACGCACACCTTCTCCAGCATCTGGG 5335 0.12229630043948728 No Hit
ACCTATGTTACACCATCTTCAGTGCCAGTCTTGGGCAAAATTGTGCAAGA 5319 0.12192952615513268 No Hit
GAGGGAGAGGCCGGAGCTGCCCAGGACAGGCTCTGGGTCCTGCCCTTGCA 5311 0.12174613901295539 No Hit
GACGCGACCGGACCGAGCGCCAAACGCGGCAGCCCAATCGCCATCGCTTT 5276 0.12094382026592969 No Hit
TAAAACTGCATCAAGTCATGGGGCATGTGGAAGGTAGGGAGGCAAGATGA 5253 0.12041658223216996 No Hit
CATGTACAAGGCCTTGCTACCTCAGCAGTCCTACAGCTTGGCCCAGCCGC 5178 0.11869732777425776 No Hit
GGTGATGCCAAGGACGATGGGATGTGGGGACCGACGTAGTGAGGTGGCGG 5082 0.11649668206813014 No Hit
CGAGCCCGGCGGGCCCTGTGATTGGACGGGCGCCCGCCTCGCGTCCCGCC 5057 0.11592359724882609 No Hit
CACCAAAAACCCCAAGACAGAAATCTTAGGTATTCAGTTTCTTTTTCACA 5010 0.11484619778853444 No Hit
CATGTAGGTTATATATGCTAGAATTGCATTTAATCACTGTGAAAAGACTG 4965 0.11381464511378714 No Hit
ATAATCCATACCACCAGTATAGCAGCAATTCTAACAGCCCCCCTACTGTC 4956 0.11360833457883766 No Hit
ATTGTTTCAAAAAAAATCAAACTGTAGTTGTTTTGGCGATAGGTCTCACG 4937 0.11317279011616659 No Hit
GTTTTTATACACTAAGGACACTCCCAGTGATGAAAATGAATTCCCCTCCA 4836 0.11085752744617818 No Hit
TGAGTAGGGGGAGCAAATCGTGCCTTGTCATTTTATTTGGAGGTTTCCTG 4710 0.1079691799568857 No Hit
TAATCTACCTGAAGTCAATGAATGCAATTTTTCACACACACACACACACA 4648 0.10654792960501161 No Hit
GCCCTCCTCCTGCCACGGGCCTGCTCCCCTCCTTCTCTCATGGGGGTCTG 4646 0.10650208281946728 No Hit
TGAGATTTTTTTGTGTATGTTTTTGACTCTTTTGAGTGGTAATCATATGT 4588 0.10517252603868185 No Hit
GCCTCAGCCTTCCTTCGCAGAAAGTTGAGCAGGTCGCCATAGCAACAGTA 4583 0.10505790907482104 No Hit
GCACGTGGGAGAGTAAGCAGGCCAGGTAGAGCTGCAGAAAACTAAGGCCC 4575 0.10487452193264374 No Hit
TAAATATAGCCCCAAAATGGTTGCTATAATAATCCCCATTTCATACTGGG 4570 0.10475990496878293 No Hit
CATGGTCACTCTCCCCAAAATATTATATTTTTTCTATAAAAAGAAAAAAA 4562 0.10457651782660561 No Hit
CATGGAGGGGAGCACTCAGCACCTCCCTCACCCCACACCCTTGGCTGCTC 4527 0.10377419907957994 No Hit
AAGTCCTCCTTATTCAAGATTTTGAAATTCTTAGCCTGGGAGTGCTGGAG 4446 0.10191740426503478 No Hit
TAACACTGATATACAAATTGGGACTCTTCATTCTGGAGAAAGCATCAGGT 4422 0.10136724283850286 No Hit
CATGACTATAAATATAAAGCAAATATGATTATGCTCACAAGCTCTGGAAT 4389 0.1006107708770215 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 2608310 3.6218064 6.263381 2
TTTTT 2247660 3.4210594 4.1753836 60-64
GGGGG 645070 3.1523657 7.9625673 4
CCCAG 820785 3.0071113 4.4379463 40-44
GGAGG 730665 2.7762291 5.3333445 4
CCAGG 719200 2.6671174 5.8472495 5
CCAGC 722800 2.648124 8.649694 5
CCCCA 711460 2.575125 5.499337 1
CCTCC 698185 2.5738966 9.818555 6
CCTGG 681120 2.5726984 5.3967257 9
CAGCC 688655 2.5230267 6.50456 6
TGGGG 618955 2.3953497 11.061625 3
GCTGG 579275 2.2147377 10.408703 7
GAGGG 572155 2.1739557 6.475631 9
GGGGA 558085 2.1204956 5.6336 7
CCACC 575140 2.0817156 5.4489 1
CTCCT 686185 2.0277488 5.7639723 4
CTCCA 682280 1.9795332 5.383313 6
GCCAG 516990 1.9172317 8.09117 4
TGGCT 632720 1.9157072 6.829514 3
TCCAG 649980 1.9088529 6.2663555 7
CACCA 664975 1.8942298 5.8541613 8
TGTTT 980205 1.8839391 5.6814566 3
CCCAC 520405 1.8836025 5.5757403 9
TGGTG 599965 1.8387208 7.553785 9
TGCAG 613635 1.8241262 5.878016 3
GGCTC 480070 1.8133005 7.274229 4
TGTGT 745515 1.8093655 5.3875203 1
GCCCA 489880 1.7947744 5.6012735 4
TCCTC 605025 1.7879126 6.6191854 5
AGGGG 453630 1.7236091 7.4619565 6
CCAGA 578770 1.6688056 5.109812 8
GAAAG 690295 1.585573 5.003869 5
GCTCC 418605 1.5620587 6.7608914 2
TGCCC 418315 1.5609765 12.114308 3
TAAAA 1080200 1.5277174 7.9771333 1
CTGGT 494570 1.4974259 6.860655 8
GATGG 496525 1.4940262 6.125909 1
ATGGA 618465 1.4469031 10.357103 2
CATGG 476895 1.4176452 43.159264 1
GCAGT 441595 1.3127104 5.3001504 4
GGGGT 336735 1.3031608 5.5582356 4
ATGTG 543810 1.2958188 7.823994 2
TGCTC 426895 1.2769277 5.081035 1
TGGGT 411185 1.2601641 6.128504 3
AGACA 544100 1.2346901 5.363069 2
ATGGG 409860 1.2332542 15.639068 2
ATGCC 418920 1.2302788 12.059661 2
ATGGC 413305 1.2286139 11.612978 2
ATGAA 667705 1.2145523 8.985095 2
TGAGA 518460 1.2129407 7.518819 1
GTCTT 483875 1.1601943 6.9007106 9
GCACC 310785 1.1386236 6.213077 8
GAGAC 387770 1.1317388 7.5093837 2
AACAC 491575 1.1020384 5.000414 2
CATGT 466920 1.099176 34.570045 1
ATGTT 563860 1.0640166 8.552984 2
CATGA 452610 1.046107 29.822948 1
GTGTC 340420 1.0307008 7.217599 2
ATGCT 432460 1.0180538 7.8980484 2
TAAAG 558290 1.0155269 6.1771812 1
ATGGT 409300 0.9753014 7.5571413 2
AGACC 338015 0.97462076 7.107697 3
CACTC 332325 0.9641912 5.3852715 1
ATGAC 416330 0.96225405 7.4010286 2
ATGAT 515405 0.95488924 6.7220535 2
ATGTA 508365 0.9418462 10.226852 2
CATGC 315455 0.9264242 33.266937 1
CGCTG 235740 0.89042735 8.563982 6
GTATG 336140 0.8009719 6.0080657 2
TAAGT 412745 0.7646914 6.347567 1
ATGCA 322640 0.74571043 9.311698 2
TAACT 405705 0.7425786 5.259252 1
TAAAC 381800 0.6861123 5.057574 1
TGCCG 167105 0.6311821 10.967205 5
CGCAC 162705 0.59610265 5.614552 7
TATGC 245105 0.5770016 5.544475 3
CCGCA 152370 0.5582383 5.8208566 6
GACCG 145660 0.5401728 6.427371 4
CCGTC 135020 0.5038381 8.599571 7
GCCGT 126865 0.47918922 8.729215 6
CGTCT 150125 0.4490537 7.4446607 8
TCGCT 146085 0.43696925 6.283803 5
ACCGC 119070 0.43623698 6.4791713 5
GTCGC 97825 0.36950055 7.8859816 4
TGTCG 118295 0.3581657 6.419491 3
CGTAT 100375 0.23629273 5.320824 1
Produced by FastQC (version 0.10.0)