##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	8016-8-16.h.sapiens.aligned.bam	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	5518418	
Filtered Sequences	0	
Sequence length	1-100	
%GC	44	
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.898698322598978	24.0	24.0	31.0	23.0	31.0
2	30.976194596373116	31.0	31.0	32.0	27.0	33.0
3	32.60103389559971	33.0	33.0	34.0	29.0	35.0
4	33.80808024126715	34.0	33.0	36.0	29.0	37.0
5	35.36336457602743	36.0	35.0	37.0	33.0	37.0
6	35.68344252016998	36.0	36.0	38.0	33.0	38.0
7	35.70865220770952	37.0	35.0	37.0	32.0	37.0
8	35.54934734665597	36.0	36.0	37.0	32.0	38.0
9	35.57010519175748	37.0	36.0	37.0	31.0	38.0
10-14	35.92874899753404	37.0	36.0	37.4	32.2	38.0
15-19	36.912298993806345	38.0	37.2	38.4	32.6	39.4
20-24	36.54561399497435	37.4	36.0	38.8	32.8	39.8
25-29	36.20313472350301	37.6	36.2	38.0	32.6	38.2
30-34	36.029865909514555	37.6	35.6	38.2	32.4	38.6
35-39	36.04888124696167	37.8	36.4	38.0	31.6	38.6
40-44	35.97485569841391	37.4	35.8	38.4	31.6	38.8
45-49	35.93487745857791	37.6	35.8	38.2	31.6	38.6
50-54	36.147717013858205	37.6	36.2	38.2	33.0	39.0
55-59	35.85494487942706	37.4	35.6	38.2	31.8	38.6
60-64	35.867879575828994	37.4	35.6	38.2	32.4	38.6
65-69	35.66561850341399	37.4	35.8	38.2	31.4	38.6
70-74	35.705103892199176	37.2	36.0	38.4	31.6	39.0
75-79	35.431284169464185	37.4	35.4	37.6	31.2	38.4
80-84	35.53651237448747	37.0	35.8	38.2	31.0	38.4
85-89	35.231985490430034	37.2	35.4	37.6	31.0	38.2
90-94	35.25031181741302	37.0	35.0	38.2	30.8	38.4
95-99	35.07322878945588	37.4	35.0	37.6	29.8	38.2
100	33.980977125883946	36.0	33.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
1	6.0
2	48.0
3	232.0
4	0.0
5	16.0
6	36.0
7	131.0
8	258.0
9	10197.0
10	566.0
11	776.0
12	1349.0
13	2827.0
14	3750.0
15	4613.0
16	4874.0
17	5903.0
18	7612.0
19	9387.0
20	11199.0
21	11516.0
22	11333.0
23	13119.0
24	15627.0
25	21098.0
26	28087.0
27	38293.0
28	52701.0
29	69661.0
30	90985.0
31	118569.0
32	155172.0
33	206114.0
34	302058.0
35	539053.0
36	1576686.0
37	2204562.0
38	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.414408299061716	18.775308744206875	22.40656591382601	38.4037170429054
2	18.879165005059274	50.32059034822206	16.291797143705978	14.508447503012684
3	18.421274365633682	35.30685689817416	30.43826997508913	15.833598761103021
4	34.197781378156414	24.722172833551056	21.10990661273242	19.97013917556011
5	20.081029262747066	27.590206002966934	26.650060185173412	25.67870454911259
6	19.91186083151414	29.053531920045415	25.927614485392215	25.106992763048225
7	21.976890184666527	28.29746693199025	27.20482842298932	22.520814460353904
8	21.674686718891873	27.128735089105184	28.863827655000357	22.33275053700259
9	21.358076501203424	28.753923836855815	27.316370628043394	22.571629033897363
10-14	21.99254477781803	27.655761698684927	26.855120585424597	23.496572938072447
15-19	21.467206845773802	27.38665214928635	27.52388600085702	23.62225500408283
20-24	21.680731507221623	26.82857599595726	27.71832144968469	23.772371047136424
25-29	21.842565004724033	27.258346519693333	28.158486258219668	22.74060221736297
30-34	22.246947458062387	27.746024960947096	27.371497972983843	22.63552960800667
35-39	22.38444748429457	26.581157836501585	28.662666898929867	22.37172778027398
40-44	22.44787472712201	27.53166822364042	27.399977590249268	22.620479458988303
45-49	21.972471564850895	28.15935508912682	27.899155187706555	21.969018158315727
50-54	22.47747761496133	27.565069982255146	27.63867870262546	22.318773700158065
55-59	21.962607918255234	28.349124046943857	27.479150038923827	22.20911799587708
60-64	22.336896062741822	27.74842226017686	28.187790300734818	21.726891376346504
65-69	22.461696497440915	28.581577401383445	27.696355357296355	21.260370743879285
70-74	22.13799190651868	28.367617937479007	27.416983085858416	22.077407070143902
75-79	22.77436623362109	28.251939984751978	27.43562758093429	21.538066200692636
80-84	21.85723968721676	28.260170247904203	27.58700855142043	22.29558151345861
85-89	22.058650583125367	28.114104663967893	27.766379584255507	22.060865168651233
90-94	22.044607992452335	27.793860889243465	27.924894521940306	22.236636596363887
95-99	22.249033966536157	26.979278955326137	28.185633026882122	22.58605405125558
100	23.385505425595024	27.18627360807592	26.82186287665582	22.606358089673233
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	58.81812534196711
2	33.38761250807196
3	34.254873126736705
4	54.16792055371652
5	45.75973381185966
6	45.01885359456237
7	44.497704645020434
8	44.00743725589446
9	43.92970553510079
10-14	45.48911771589047
15-19	45.08946184985663
20-24	45.45310255435805
25-29	44.583167222087
30-34	44.88247706606906
35-39	44.75617526456855
40-44	45.06835418611031
45-49	43.94148972316662
50-54	44.79625131511939
55-59	44.17172591413232
60-64	44.06378743908832
65-69	43.722067241320204
70-74	44.21539897666258
75-79	44.312432434313735
80-84	44.15282120067537
85-89	44.1195157517766
90-94	44.28124458881623
95-99	44.83508801779173
100	45.991863515268264
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	11037.0
1	11039.5
2	11039.5
3	11042.0
4	11047.0
5	11050.5
6	11060.0
7	11076.0
8	11094.0
9	11107.0
10	11119.0
11	11144.0
12	11183.5
13	11217.0
14	11254.5
15	11941.0
16	12304.5
17	11938.0
18	12281.0
19	13320.5
20	14465.0
21	16017.5
22	20093.5
23	26644.5
24	41073.0
25	54691.5
26	62877.5
27	75235.0
28	83254.5
29	99493.5
30	124668.5
31	139694.5
32	146128.0
33	159475.5
34	177780.0
35	175871.0
36	166686.5
37	160103.5
38	183964.0
39	205163.0
40	210259.0
41	224037.5
42	218773.0
43	223036.5
44	227296.0
45	210280.5
46	193981.0
47	173849.5
48	164228.5
49	175829.5
50	182857.5
51	188106.0
52	157831.0
53	101946.0
54	87531.5
55	90944.0
56	73114.5
57	61693.5
58	82599.0
59	80502.0
60	91240.0
61	124888.0
62	117132.5
63	106459.5
64	89968.0
65	72403.0
66	69067.0
67	61566.5
68	50890.5
69	45582.0
70	41688.5
71	36849.0
72	41700.0
73	30760.0
74	15135.0
75	18446.0
76	20710.0
77	20853.5
78	18688.0
79	16954.0
80	15904.5
81	14922.5
82	13629.5
83	12827.0
84	13720.0
85	13527.0
86	13333.0
87	12036.0
88	11011.0
89	11137.5
90	11746.0
91	12446.0
92	9089.0
93	5637.0
94	5543.0
95	5544.0
96	5538.5
97	5526.0
98	5509.5
99	5501.5
100	5497.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.033216041263999935
2	0.007935134542294359
3	0.0019066853654997812
4	0.006937138807970736
5	0.005249804403913774
6	8.72549431288641E-4
7	2.5497808281251026E-4
8	1.2749981603597972E-4
9	3.642878427675385E-5
10-14	0.0011839675349544557
15-19	0.01680883119156467
20-24	0.015487224821986037
25-29	0.008217281778609949
30-34	0.008030876130502034
35-39	0.00526968403692435
40-44	0.012203821152000706
45-49	0.011490251497052182
50-54	0.010435745987987875
55-59	0.008971518205168269
60-64	0.040475461249558045
65-69	0.035648981685620945
70-74	0.02254470418349045
75-79	0.05142932037888559
80-84	0.04831117009032343
85-89	0.011345046003087571
90-94	0.0011867499485741688
95-99	0.00870847766535108
100	0.008152689021902219
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
0-1	11265.0
2-3	560.0
4-5	5472.0
6-7	10917.0
8-9	72.0
10-11	72.0
12-13	164.0
14-15	406.0
16-17	308.0
18-19	5150.0
20-21	62277.0
22-23	3172.0
24-25	5056.0
26-27	1301.0
28-29	1064.0
30-31	2120.0
32-33	1920.0
34-35	1883.0
36-37	1342.0
38-39	1670.0
40-41	4898.0
42-43	2387.0
44-45	1550.0
46-47	2112.0
48-49	1548.0
50-51	20767.0
52-53	2138.0
54-55	22503.0
56-57	16936.0
58-59	45452.0
60-61	1900.0
62-63	10832.0
64-65	7012.0
66-67	8398.0
68-69	6473.0
70-71	27686.0
72-73	22322.0
74-75	15081.0
76-77	16979.0
78-79	6995.0
80-81	10345.0
82-83	15606.0
84-85	8791.0
86-87	2936.0
88-89	6509.0
90-91	11257.0
92-93	1626.0
94-95	4243.0
96-97	116993.0
98-99	0.0
100-101	4979952.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	97.1224588612045
#Duplication Level	Relative count
1	100.0
2	6.5386601508873134
3	2.04730657329622
4	1.117800420670806
5	0.8866833759461944
6	0.807335289746464
7	0.8199302240638815
8	0.7953701021449173
9	0.8734586949129061
10++	11.763038905752106
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TGAGACCGCACCAGCGACACA	20136	0.36488718324708275	No Hit
TGTGTCGCTGGTGCGGTCTCA	19862	0.35992199213615206	No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA	11930	0.21618514581534057	No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC	11878	0.21524284677239022	No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC	11241	0.2036996834962484	No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG	11131	0.20170635859769956	No Hit
CACTCAGTATGAGGAGCCGGCTGTCATCACATAGGGACAGGGCTCTCCCT	10696	0.19382366468071102	No Hit
CATGCCCTCCAGCGGCCCCGGGGACACCAGCAGCTCTGCTGCGGAGCGGG	10624	0.19251894292893362	No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA	10404	0.18853229313183598	No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG	10254	0.18581412281563303	No Hit
TAAAGAAACCCACAGAAACACACACACATACAATTAGCAAATGAATTCAA	10085	0.1827516509260444	No Hit
AAAAATAATAAACCGTTCATTTCTCAGGATGTGGTCATAGAATAAAGTTA	10063	0.18235298594633462	No Hit
*	9842	0.1783482150137956	No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT	9792	0.177442158241728	No Hit
GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG	9737	0.17644549579245355	No Hit
GATACAACATTCAAGACAGAGGAACTGGATGCCAAGCCAGACACAGACCC	9374	0.16986752362724244	No Hit
CATGTATTTTGAACCGGCACCCCTGTTACCACAGAGTGTGGGAGGAACTG	9194	0.1666057192477989	No Hit
CATGGGGTTGTGCCAATTAATTACCAAACATTGAGCCTGCAGGCTTTGAG	8905	0.16136871110524792	No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG	8889	0.16107877293818626	No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA	8815	0.15973780891552616	No Hit
AAGACAGGGGAAAGGAAGAAAAGGGAGTGAAGAGGGAATGGGAGAGGGAA	8629	0.1563672777234345	No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT	8484	0.15373971308443835	No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT	8386	0.15196384181118575	No Hit
GACGCGACCGGACCGAGCGCCAAACGCGGCAGCCCAATCGCCATCGCTTT	8327	0.15089469482014592	No Hit
CATGTAGACTTACCTTTCCTCATAGAGCTATCCTGGTTAATAACAGGCCA	7822	0.14174352142226268	No Hit
CGAGCCCGGCGGGCCCTGTGATTGGACGGGCGCCCGCCTCGCGTCCCGCC	7812	0.14156231006784917	No Hit
ACACTTACACCAAGGAATCTCCCAGGCATTATCAAAGCTACAGGAGACAA	7777	0.1409280703274018	No Hit
AGCAACAAGAACAATACAAACCTCATTTCCTTTAGTCCTTCTGCCTCTAT	7728	0.1400401346907755	No Hit
TAACTGTGGAAAAACTAAGTTGTCACCAGCGGTACCTCATAGCATAACTT	7718	0.139858923336362	No Hit
CATGTAATTCAGGCAGTTATGGGAGCCCTAGAGGGCCTGAGAGTTGCTATTGGAC	7508	0.13605348489367786	No Hit
GTCCAATAGCAACTCTCAGGCCCTCTAGGGCTCCCATAACTGCCTGAATTACATG	7472	0.13540112401778914	No Hit
CATGTACAAGGCCTTGCTACCTCAGCAGTCCTACAGCTTGGCCCAGCCGC	7091	0.12849697141463368	No Hit
GGTGATGCCAAGGACGATGGGATGTGGGGACCGACGTAGTGAGGTGGCGG	6884	0.12474589637827363	No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA	6794	0.12311499418855186	No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC	6777	0.12280693488604887	No Hit
ACCTATGTTACACCATCTTCAGTGCCAGTCTTGGGCAAAATTGTGCAAGA	6580	0.11923707120410232	No Hit
CATGGAATATATATTCTCCTGGCTGGTGCTTATGATTGCAAAACTTGGAT	6510	0.11796859172320763	No Hit
AACTTTATAAACCAACTTCTGCTAGCTTCCAACTTCTCTTCTGCAGCTTC	6492	0.11764241128526327	No Hit
CACACTGTCATTAGTTGCAGCATCTCCTGTAGCAATTGGGCTAAATCTGT	6484	0.11749744220173246	No Hit
ACAGATTTTGGGGTTGTGTTGTCACCCAAGAGATTGTTGTTTGCCATACT	6446	0.11680883905496105	No Hit
TAATCCAGAGCAAGGCCTTGACTCCCTTCAGTTCTATGAAGAGAATGAGG	6424	0.11641017407525128	No Hit
TAAAACTGCATCAAGTCATGGGGCATGTGGAAGGTAGGGAGGCAAGATGA	6422	0.11637393180436859	No Hit
TGAGTAGGGGGAGCAAATCGTGCCTTGTCATTTTATTTGGAGGTTTCCTG	6409	0.11613835704363097	No Hit
GACAACTTGGCCACCGACAGCTGAAGGGCTTTTCACCTGTTGACACAATTGCCAGTCA	6197	0.11229667633006417	No Hit
TGACTGGCAATTGTGTCAACAGGTGAAAAGCCCTTCAGCTGTCGGTGGCCAAGTTGTC	6157	0.11157183091241003	No Hit
GAGACCGCACCAGCGACACC	6060	0.1098140807745988	No Hit
ATTGTTTCAAAAAAAATCAAACTGTAGTTGTTTTGGCGATAGGTCTCACG	6018	0.10905299308606199	No Hit
GCCTCAGCCTTCCTTCGCAGAAAGTTGAGCAGGTCGCCATAGCAACAGTA	5905	0.1070053047811891	No Hit
ATCCTTCACAAAGTCTTTGAGATATATTTTTATCAAATATTTAGCATGGATCCCGGTACACTTTCAATACTTA	5873	0.10642542844706582	No Hit
GGTGTCGCTGGTGCGGTCTC	5866	0.10629858049897635	No Hit
CGAGACCGCGCCCCAGGCCCAGCACTCACAAATTCCTGGTCGTGGTTATT	5827	0.10559185621676356	No Hit
TAAGTATTGAAAGTGTACCGGGATCCATGCTAAATATTTGATAAAAATATATCTCAAAGACTTTGTGAAGGAT	5813	0.10533816032058463	No Hit
CATGGGCTCGGCCACGCGCTACCACACCTACCTGCCGCCGCCCTACCCCG	5773	0.10461331490293051	No Hit
TAATCTACCTGAAGTCAATGAATGCAATTTTTCACACACACACACACACA	5669	0.1027287168170298	No Hit
GAGAACTGGTAGGAGCCGGCCGAGGCGCCGTAGTACAGGTGGTAGGAGGG	5648	0.1023481729727614	No Hit
GGCACAAGGGTACCTACGGGCTGCTGCGGCGGCGAGAGGACTGGCCCTCC	5647	0.10233005183732005	No Hit
GAGGGAGAGGCCGGAGCTGCCCAGGACAGGCTCTGGGTCCTGCCCTTGCA	5619	0.10182266004496215	No Hit
GTTTTTATACACTAAGGACACTCCCAGTGATGAAAATGAATTCCCCTCCA	5531	0.10022800012612311	No Hit
CATGGCTCCACTCTCCACCGTCTCCACGCACACCTTCTCCAGCATCTGGG	5521	0.10004678877170957	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	3047910	3.51694	6.19009	2
TTTTT	2730910	3.4259126	4.249705	60-64
GGGGG	806555	2.988885	6.904837	4
CCAGG	939820	2.6692624	5.770649	5
CCAGC	945165	2.640956	8.448287	5
CCTGG	890685	2.572361	5.8210735	9
CAGCC	912775	2.5504525	6.7420073	6
CCTCC	911330	2.5474012	9.329817	6
CCCCA	926285	2.546274	5.2758923	1
TGGGG	799455	2.3855472	9.509164	3
GCTGG	751215	2.205287	11.502059	7
GAGGG	728655	2.1382315	6.455551	9
GGGGA	706580	2.0734525	5.2072353	7
CCACC	754145	2.0730765	5.170196	1
CCCTC	724120	2.0241013	6.0881853	5
AAGAA	1378395	2.0085332	5.0145955	2
CTCCA	887755	1.9974015	5.02564	7
TCCAG	848405	1.9402986	6.1702104	7
GCCAG	680760	1.9334842	7.9110575	4
CACCA	867740	1.9199975	6.0353694	8
TGGCT	810275	1.9153672	6.2259192	3
CCCAC	688300	1.8920746	5.287872	9
TGGTG	785835	1.8881829	8.503293	9
TGTTT	1184790	1.845829	5.5260468	3
TGCAG	793245	1.8440206	5.1060195	3
GCCCA	653205	1.8251687	5.65003	4
TCCTC	796670	1.8226863	5.222111	5
GGCTC	615740	1.7783006	6.958493	1
TGTGT	909545	1.7597686	6.076893	1
AGGGG	578780	1.6984247	7.036976	6
TGCCC	547205	1.5547651	12.285007	3
GCTCC	541895	1.539678	7.619805	2
GATGG	649980	1.5358593	5.7906547	1
CTGGT	648720	1.5334756	7.8229804	8
TAAAA	1296650	1.5214119	8.079965	1
ATGGA	783970	1.4669267	9.90697	2
CATGG	610145	1.4183763	38.389645	1
GGGGT	441630	1.3178092	5.359071	4
ATGTG	678805	1.291562	7.5777664	2
TGCTC	546030	1.2698226	5.808941	1
TGAGA	675105	1.2632238	8.865847	1
TGGGT	524835	1.2610592	5.739435	3
GGCAC	440995	1.2525073	5.0787215	1
AGACA	689295	1.2478427	5.1359844	2
ATGGG	525565	1.241875	13.635347	2
ATGGC	533620	1.2404822	10.586564	2
ATGAA	827515	1.2261466	8.704797	2
ATGCC	522305	1.1945094	11.995288	2
GAGAC	508130	1.1616414	9.190568	2
GTCTT	601930	1.1457354	5.0410776	9
GCACC	399295	1.1156999	7.149388	8
CATGT	576185	1.078547	30.919344	1
ATGTT	696315	1.0668286	7.445876	2
GTGTC	449520	1.0625972	8.372576	2
CATGA	573885	1.0564302	27.76648	1
TAAAG	702190	1.0404499	6.363757	1
AGACC	449725	1.0114657	8.583157	3
ATGCT	528340	0.9889871	7.523538	2
ATGGT	515715	0.9812507	6.7524476	2
CACTC	434800	0.97827685	5.753496	1
ATGAT	645300	0.9722756	6.4559064	2
ATGAC	515440	0.94884235	6.904005	2
ATGTA	618795	0.93234044	9.284468	2
CATGC	400200	0.9152557	31.08147	1
GCGCC	251890	0.888806	5.522551	8
CGCTG	301235	0.86998785	9.655695	6
TAAGA	555560	0.823185	5.28128	1
TAAGT	501000	0.7548583	6.7544336	1
ATGCA	406715	0.74869704	8.499271	2
TAACT	502375	0.74466795	5.5950785	1
TAAAC	482320	0.70308644	5.477323	1
CGCAC	215995	0.6035277	6.561696	7
TGCCG	203775	0.58851653	7.844485	5
CCGCA	205730	0.5748455	6.866547	6
GACCG	197370	0.56056726	7.9835873	4
TCGCT	199515	0.46398303	7.2095585	5
CCGTC	161635	0.45925105	5.549719	7
ACCGC	161190	0.450393	8.004597	5
GCCGT	153515	0.44336212	5.557958	6
TGTCG	157705	0.37279066	7.45069	3
GTCGC	126780	0.3661496	8.934838	4
>>END_MODULE