FastQCFastQC Report
Wed 20 Mar 2013
8016-8-16.h.sapiens.aligned.bam

Summary

[OK] Basic Statistics

Measure Value
Filename 8016-8-16.h.sapiens.aligned.bam
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 5518418
Filtered Sequences 0
Sequence length 1-100
%GC 44

[WARN] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
TGAGACCGCACCAGCGACACA 20136 0.36488718324708275 No Hit
TGTGTCGCTGGTGCGGTCTCA 19862 0.35992199213615206 No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA 11930 0.21618514581534057 No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC 11878 0.21524284677239022 No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC 11241 0.2036996834962484 No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG 11131 0.20170635859769956 No Hit
CACTCAGTATGAGGAGCCGGCTGTCATCACATAGGGACAGGGCTCTCCCT 10696 0.19382366468071102 No Hit
CATGCCCTCCAGCGGCCCCGGGGACACCAGCAGCTCTGCTGCGGAGCGGG 10624 0.19251894292893362 No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA 10404 0.18853229313183598 No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG 10254 0.18581412281563303 No Hit
TAAAGAAACCCACAGAAACACACACACATACAATTAGCAAATGAATTCAA 10085 0.1827516509260444 No Hit
AAAAATAATAAACCGTTCATTTCTCAGGATGTGGTCATAGAATAAAGTTA 10063 0.18235298594633462 No Hit
* 9842 0.1783482150137956 No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT 9792 0.177442158241728 No Hit
GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG 9737 0.17644549579245355 No Hit
GATACAACATTCAAGACAGAGGAACTGGATGCCAAGCCAGACACAGACCC 9374 0.16986752362724244 No Hit
CATGTATTTTGAACCGGCACCCCTGTTACCACAGAGTGTGGGAGGAACTG 9194 0.1666057192477989 No Hit
CATGGGGTTGTGCCAATTAATTACCAAACATTGAGCCTGCAGGCTTTGAG 8905 0.16136871110524792 No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG 8889 0.16107877293818626 No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA 8815 0.15973780891552616 No Hit
AAGACAGGGGAAAGGAAGAAAAGGGAGTGAAGAGGGAATGGGAGAGGGAA 8629 0.1563672777234345 No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT 8484 0.15373971308443835 No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT 8386 0.15196384181118575 No Hit
GACGCGACCGGACCGAGCGCCAAACGCGGCAGCCCAATCGCCATCGCTTT 8327 0.15089469482014592 No Hit
CATGTAGACTTACCTTTCCTCATAGAGCTATCCTGGTTAATAACAGGCCA 7822 0.14174352142226268 No Hit
CGAGCCCGGCGGGCCCTGTGATTGGACGGGCGCCCGCCTCGCGTCCCGCC 7812 0.14156231006784917 No Hit
ACACTTACACCAAGGAATCTCCCAGGCATTATCAAAGCTACAGGAGACAA 7777 0.1409280703274018 No Hit
AGCAACAAGAACAATACAAACCTCATTTCCTTTAGTCCTTCTGCCTCTAT 7728 0.1400401346907755 No Hit
TAACTGTGGAAAAACTAAGTTGTCACCAGCGGTACCTCATAGCATAACTT 7718 0.139858923336362 No Hit
CATGTAATTCAGGCAGTTATGGGAGCCCTAGAGGGCCTGAGAGTTGCTATTGGAC 7508 0.13605348489367786 No Hit
GTCCAATAGCAACTCTCAGGCCCTCTAGGGCTCCCATAACTGCCTGAATTACATG 7472 0.13540112401778914 No Hit
CATGTACAAGGCCTTGCTACCTCAGCAGTCCTACAGCTTGGCCCAGCCGC 7091 0.12849697141463368 No Hit
GGTGATGCCAAGGACGATGGGATGTGGGGACCGACGTAGTGAGGTGGCGG 6884 0.12474589637827363 No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA 6794 0.12311499418855186 No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC 6777 0.12280693488604887 No Hit
ACCTATGTTACACCATCTTCAGTGCCAGTCTTGGGCAAAATTGTGCAAGA 6580 0.11923707120410232 No Hit
CATGGAATATATATTCTCCTGGCTGGTGCTTATGATTGCAAAACTTGGAT 6510 0.11796859172320763 No Hit
AACTTTATAAACCAACTTCTGCTAGCTTCCAACTTCTCTTCTGCAGCTTC 6492 0.11764241128526327 No Hit
CACACTGTCATTAGTTGCAGCATCTCCTGTAGCAATTGGGCTAAATCTGT 6484 0.11749744220173246 No Hit
ACAGATTTTGGGGTTGTGTTGTCACCCAAGAGATTGTTGTTTGCCATACT 6446 0.11680883905496105 No Hit
TAATCCAGAGCAAGGCCTTGACTCCCTTCAGTTCTATGAAGAGAATGAGG 6424 0.11641017407525128 No Hit
TAAAACTGCATCAAGTCATGGGGCATGTGGAAGGTAGGGAGGCAAGATGA 6422 0.11637393180436859 No Hit
TGAGTAGGGGGAGCAAATCGTGCCTTGTCATTTTATTTGGAGGTTTCCTG 6409 0.11613835704363097 No Hit
GACAACTTGGCCACCGACAGCTGAAGGGCTTTTCACCTGTTGACACAATTGCCAGTCA 6197 0.11229667633006417 No Hit
TGACTGGCAATTGTGTCAACAGGTGAAAAGCCCTTCAGCTGTCGGTGGCCAAGTTGTC 6157 0.11157183091241003 No Hit
GAGACCGCACCAGCGACACC 6060 0.1098140807745988 No Hit
ATTGTTTCAAAAAAAATCAAACTGTAGTTGTTTTGGCGATAGGTCTCACG 6018 0.10905299308606199 No Hit
GCCTCAGCCTTCCTTCGCAGAAAGTTGAGCAGGTCGCCATAGCAACAGTA 5905 0.1070053047811891 No Hit
ATCCTTCACAAAGTCTTTGAGATATATTTTTATCAAATATTTAGCATGGATCCCGGTACACTTTCAATACTTA 5873 0.10642542844706582 No Hit
GGTGTCGCTGGTGCGGTCTC 5866 0.10629858049897635 No Hit
CGAGACCGCGCCCCAGGCCCAGCACTCACAAATTCCTGGTCGTGGTTATT 5827 0.10559185621676356 No Hit
TAAGTATTGAAAGTGTACCGGGATCCATGCTAAATATTTGATAAAAATATATCTCAAAGACTTTGTGAAGGAT 5813 0.10533816032058463 No Hit
CATGGGCTCGGCCACGCGCTACCACACCTACCTGCCGCCGCCCTACCCCG 5773 0.10461331490293051 No Hit
TAATCTACCTGAAGTCAATGAATGCAATTTTTCACACACACACACACACA 5669 0.1027287168170298 No Hit
GAGAACTGGTAGGAGCCGGCCGAGGCGCCGTAGTACAGGTGGTAGGAGGG 5648 0.1023481729727614 No Hit
GGCACAAGGGTACCTACGGGCTGCTGCGGCGGCGAGAGGACTGGCCCTCC 5647 0.10233005183732005 No Hit
GAGGGAGAGGCCGGAGCTGCCCAGGACAGGCTCTGGGTCCTGCCCTTGCA 5619 0.10182266004496215 No Hit
GTTTTTATACACTAAGGACACTCCCAGTGATGAAAATGAATTCCCCTCCA 5531 0.10022800012612311 No Hit
CATGGCTCCACTCTCCACCGTCTCCACGCACACCTTCTCCAGCATCTGGG 5521 0.10004678877170957 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 3047910 3.51694 6.19009 2
TTTTT 2730910 3.4259126 4.249705 60-64
GGGGG 806555 2.988885 6.904837 4
CCAGG 939820 2.6692624 5.770649 5
CCAGC 945165 2.640956 8.448287 5
CCTGG 890685 2.572361 5.8210735 9
CAGCC 912775 2.5504525 6.7420073 6
CCTCC 911330 2.5474012 9.329817 6
CCCCA 926285 2.546274 5.2758923 1
TGGGG 799455 2.3855472 9.509164 3
GCTGG 751215 2.205287 11.502059 7
GAGGG 728655 2.1382315 6.455551 9
GGGGA 706580 2.0734525 5.2072353 7
CCACC 754145 2.0730765 5.170196 1
CCCTC 724120 2.0241013 6.0881853 5
AAGAA 1378395 2.0085332 5.0145955 2
CTCCA 887755 1.9974015 5.02564 7
TCCAG 848405 1.9402986 6.1702104 7
GCCAG 680760 1.9334842 7.9110575 4
CACCA 867740 1.9199975 6.0353694 8
TGGCT 810275 1.9153672 6.2259192 3
CCCAC 688300 1.8920746 5.287872 9
TGGTG 785835 1.8881829 8.503293 9
TGTTT 1184790 1.845829 5.5260468 3
TGCAG 793245 1.8440206 5.1060195 3
GCCCA 653205 1.8251687 5.65003 4
TCCTC 796670 1.8226863 5.222111 5
GGCTC 615740 1.7783006 6.958493 1
TGTGT 909545 1.7597686 6.076893 1
AGGGG 578780 1.6984247 7.036976 6
TGCCC 547205 1.5547651 12.285007 3
GCTCC 541895 1.539678 7.619805 2
GATGG 649980 1.5358593 5.7906547 1
CTGGT 648720 1.5334756 7.8229804 8
TAAAA 1296650 1.5214119 8.079965 1
ATGGA 783970 1.4669267 9.90697 2
CATGG 610145 1.4183763 38.389645 1
GGGGT 441630 1.3178092 5.359071 4
ATGTG 678805 1.291562 7.5777664 2
TGCTC 546030 1.2698226 5.808941 1
TGAGA 675105 1.2632238 8.865847 1
TGGGT 524835 1.2610592 5.739435 3
GGCAC 440995 1.2525073 5.0787215 1
AGACA 689295 1.2478427 5.1359844 2
ATGGG 525565 1.241875 13.635347 2
ATGGC 533620 1.2404822 10.586564 2
ATGAA 827515 1.2261466 8.704797 2
ATGCC 522305 1.1945094 11.995288 2
GAGAC 508130 1.1616414 9.190568 2
GTCTT 601930 1.1457354 5.0410776 9
GCACC 399295 1.1156999 7.149388 8
CATGT 576185 1.078547 30.919344 1
ATGTT 696315 1.0668286 7.445876 2
GTGTC 449520 1.0625972 8.372576 2
CATGA 573885 1.0564302 27.76648 1
TAAAG 702190 1.0404499 6.363757 1
AGACC 449725 1.0114657 8.583157 3
ATGCT 528340 0.9889871 7.523538 2
ATGGT 515715 0.9812507 6.7524476 2
CACTC 434800 0.97827685 5.753496 1
ATGAT 645300 0.9722756 6.4559064 2
ATGAC 515440 0.94884235 6.904005 2
ATGTA 618795 0.93234044 9.284468 2
CATGC 400200 0.9152557 31.08147 1
GCGCC 251890 0.888806 5.522551 8
CGCTG 301235 0.86998785 9.655695 6
TAAGA 555560 0.823185 5.28128 1
TAAGT 501000 0.7548583 6.7544336 1
ATGCA 406715 0.74869704 8.499271 2
TAACT 502375 0.74466795 5.5950785 1
TAAAC 482320 0.70308644 5.477323 1
CGCAC 215995 0.6035277 6.561696 7
TGCCG 203775 0.58851653 7.844485 5
CCGCA 205730 0.5748455 6.866547 6
GACCG 197370 0.56056726 7.9835873 4
TCGCT 199515 0.46398303 7.2095585 5
CCGTC 161635 0.45925105 5.549719 7
ACCGC 161190 0.450393 8.004597 5
GCCGT 153515 0.44336212 5.557958 6
TGTCG 157705 0.37279066 7.45069 3
GTCGC 126780 0.3661496 8.934838 4
Produced by FastQC (version 0.10.0)