##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	8016-8-17.h.sapiens.aligned.bam	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	6718866	
Filtered Sequences	0	
Sequence length	1-100	
%GC	43	
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.89610657512741	24.0	24.0	31.0	23.0	31.0
2	30.985682720178772	31.0	31.0	32.0	27.0	33.0
3	32.61828279578697	33.0	33.0	34.0	29.0	35.0
4	33.823728762490276	34.0	33.0	36.0	29.0	37.0
5	35.37564952603123	36.0	35.0	37.0	33.0	37.0
6	35.69012525821599	36.0	36.0	38.0	33.0	38.0
7	35.72145033830897	37.0	35.0	37.0	32.0	37.0
8	35.560738220166506	36.0	36.0	37.0	32.0	38.0
9	35.579900910258985	37.0	36.0	37.0	31.0	38.0
10-14	35.94180781536916	37.0	36.0	37.4	32.2	38.0
15-19	36.92871977056422	38.0	37.2	38.4	32.6	39.4
20-24	36.570169068224075	37.4	36.0	38.8	32.8	39.8
25-29	36.227058894157366	37.6	36.2	38.0	32.8	38.2
30-34	36.06142655880915	37.6	35.6	38.2	32.4	38.6
35-39	36.092541771048545	37.8	36.4	38.0	31.6	38.6
40-44	36.02588767210755	37.4	35.8	38.4	31.8	38.8
45-49	35.97386848103044	37.6	35.8	38.2	31.6	38.6
50-54	36.1869241515213	37.6	36.2	38.2	33.0	39.0
55-59	35.896464961711914	37.4	35.6	38.2	31.8	38.6
60-64	35.90486026398724	37.6	35.6	38.2	32.6	38.6
65-69	35.71396372576807	37.4	35.8	38.2	31.4	38.6
70-74	35.753163511001496	37.2	36.0	38.4	31.6	39.0
75-79	35.46467330117247	37.4	35.4	37.6	31.4	38.4
80-84	35.579909999830534	37.0	35.8	38.2	31.0	38.6
85-89	35.29318587787194	37.2	35.4	37.6	31.0	38.2
90-94	35.31926957898812	37.0	35.0	38.2	30.8	38.4
95-99	35.1453236117441	37.4	35.2	37.6	30.8	38.2
100	34.071427191329754	36.0	33.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	2.0
1	20.0
2	47.0
3	281.0
4	1.0
5	20.0
6	34.0
7	167.0
8	320.0
9	12508.0
10	809.0
11	1062.0
12	1667.0
13	3249.0
14	4120.0
15	4861.0
16	5582.0
17	6783.0
18	8333.0
19	10267.0
20	12057.0
21	12960.0
22	13424.0
23	15413.0
24	19047.0
25	25193.0
26	34062.0
27	46768.0
28	63273.0
29	84228.0
30	110789.0
31	144406.0
32	185475.0
33	247127.0
34	362197.0
35	643217.0
36	1902092.0
37	2736996.0
38	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.085012835706536	19.81044507857171	22.034853329227815	39.06968875649394
2	17.47889584240638	52.81845651797806	15.582668639370961	14.119979000244602
3	17.91309416315345	35.886355236534705	31.034909634789535	15.165640965522304
4	34.04594767070836	25.17397301102418	21.156639705138684	19.623439613128777
5	19.874525680480446	27.980444590516594	27.48437359945024	24.66065612955272
6	19.36378040837874	30.06823261451676	26.634004742260174	23.93398223484432
7	21.83548463497837	28.702128685256223	27.85337034596188	21.609016333803527
8	20.844134203172036	27.879389380178544	29.620873374030545	21.655603042618882
9	21.153273192380283	29.299730296960803	27.59211510124419	21.95488140941472
10-14	21.594424651060827	28.09615065760252	27.4216457651071	22.88777892622956
15-19	20.948660622012785	27.957987015469023	28.015576021262014	23.077776341256182
20-24	21.240676974604053	27.41244714652883	28.190546552647362	23.156329326219755
25-29	21.459639575728893	27.522828391618404	28.780972341583293	22.236559691069413
30-34	21.899307305517222	28.031052706219985	27.983592052820928	22.08604793544187
35-39	21.91039006904651	26.824019195664935	29.213925237901435	22.05166549738712
40-44	22.022235943200496	27.929340856711704	27.79700061385971	22.25142258622809
45-49	21.627238056789146	28.71070972714152	28.32745815329248	21.33459406277686
50-54	22.037704424280257	27.97267603718238	28.07618459042624	21.91343494811112
55-59	21.397446358449944	28.829649322362233	27.92833417959526	21.844570139592566
60-64	21.8384494436822	28.289255914410948	28.663916856443816	21.208377785463036
65-69	21.84010192578429	29.10483294926987	28.254090919659692	20.80097420528615
70-74	21.629189659069002	28.755482690703765	27.995638637202163	21.61968901302507
75-79	22.267148035551703	28.71075381208002	27.952952501201985	21.069145651166295
80-84	21.52601470356606	28.617956155661734	28.21745083906091	21.6385783017113
85-89	21.621727773828024	28.31759144139842	28.47048967707289	21.59019110770067
90-94	21.501365282837305	28.17906794818948	28.52891563517189	21.790651133801326
95-99	21.745435944116316	27.34395682355534	28.74250952325683	22.168097709071514
100	23.08745858780706	27.57502089189395	27.220189001450045	22.11733151884895
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	58.154701592200475
2	31.598874842650982
3	33.07873512867575
4	53.66938728383713
5	44.535181810033166
6	43.29776264322307
7	43.444500968781895
8	42.49973724579092
9	43.10815460179501
10-14	44.482203577290385
15-19	44.026436963268964
20-24	44.39700630082381
25-29	43.69619926679831
30-34	43.98535524095909
35-39	43.962055566433634
40-44	44.27365852942859
45-49	42.961832119566004
50-54	43.95113937239138
55-59	43.24201649804251
60-64	43.04682722914524
65-69	42.641076131070434
70-74	43.24887867209407
75-79	43.336293686717994
80-84	43.16459300527736
85-89	43.211918881528696
90-94	43.29201641663863
95-99	43.913533653187834
100	45.204790106656006
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	13233.0
1	13233.5
2	13236.5
3	13238.0
4	13241.0
5	13252.0
6	13263.5
7	13276.0
8	13288.0
9	13318.5
10	13364.5
11	13397.0
12	13448.5
13	13516.5
14	13542.0
15	14410.0
16	14936.0
17	14508.5
18	14959.0
19	16374.5
20	17864.0
21	19578.5
22	25468.0
23	35596.0
24	56798.0
25	77906.5
26	89305.0
27	104788.0
28	113610.0
29	134116.0
30	169048.0
31	186704.0
32	193717.5
33	213672.5
34	239248.5
35	234539.0
36	217293.0
37	206855.0
38	235436.0
39	259964.0
40	265470.5
41	276803.5
42	261979.0
43	271382.5
44	277252.0
45	252423.0
46	231163.0
47	202815.5
48	189489.0
49	205690.5
50	216780.5
51	222154.0
52	185945.0
53	116550.5
54	99611.5
55	100149.5
56	77522.5
57	69511.5
58	93380.5
59	88584.5
60	99617.5
61	136953.5
62	123747.0
63	113723.0
64	97228.5
65	75794.5
66	75520.0
67	69183.0
68	55583.5
69	48057.5
70	44147.5
71	38736.5
72	44017.0
73	33705.5
74	18632.5
75	22640.0
76	24278.0
77	23004.0
78	20867.5
79	19770.0
80	18341.0
81	16766.5
82	15388.0
83	15040.0
84	15150.5
85	14110.0
86	16668.5
87	16258.0
88	14278.5
89	15229.0
90	16604.5
91	18120.0
92	15839.0
93	13574.0
94	13567.5
95	13547.5
96	13530.5
97	13522.5
98	13524.5
99	13521.5
100	13514.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05432464347406244
2	0.00721814486518474
3	0.0020134777727714268
4	0.007323857862265829
5	0.004660812367644879
6	9.272072055365637E-4
7	3.44334831189849E-4
8	1.1977385498440395E-4
9	0.0
10-14	0.0012606625551300312
15-19	0.017173401820883755
20-24	0.01549115685726994
25-29	0.008571445452021037
30-34	0.007698270020747429
35-39	0.0051611310577751755
40-44	0.012130527267612098
45-49	0.01115289087005745
50-54	0.010095589484766037
55-59	0.010743415731852472
60-64	0.05321921622461666
65-69	0.04305889597290613
70-74	0.026830551402584293
75-79	0.07390438441230103
80-84	0.06399473192785383
85-89	0.014754541741754548
90-94	0.0072701131125879614
95-99	0.009421300680648224
100	0.00804231944456142
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
0-1	13542.0
2-3	1207.0
4-5	17370.0
6-7	7493.0
8-9	70.0
10-11	159.0
12-13	128.0
14-15	356.0
16-17	314.0
18-19	5551.0
20-21	63769.0
22-23	3787.0
24-25	3508.0
26-27	1391.0
28-29	1571.0
30-31	2821.0
32-33	1470.0
34-35	2011.0
36-37	1437.0
38-39	2005.0
40-41	4805.0
42-43	2464.0
44-45	2392.0
46-47	3368.0
48-49	2040.0
50-51	26411.0
52-53	3632.0
54-55	33871.0
56-57	22607.0
58-59	58375.0
60-61	2846.0
62-63	11454.0
64-65	7552.0
66-67	9563.0
68-69	7096.0
70-71	33378.0
72-73	29587.0
74-75	18347.0
76-77	24754.0
78-79	8389.0
80-81	17034.0
82-83	18971.0
84-85	8806.0
86-87	2902.0
88-89	7928.0
90-91	16731.0
92-93	2350.0
94-95	4601.0
96-97	141185.0
98-99	0.0
100-101	6055467.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	97.20400577013208
#Duplication Level	Relative count
1	100.0
2	6.886022961617778
3	2.1040967712148726
4	0.9738680784265443
5	0.6623780356428329
6	0.5183292806796146
7	0.4721598079349934
8	0.48016251654406106
9	0.530025547108252
10++	10.491550986487734
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TGAGACCGCACCAGCGACACA	21158	0.31490433058197614	No Hit
TGTGTCGCTGGTGCGGTCTCA	20812	0.30975465204991437	No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA	15943	0.23728706600191163	No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC	15806	0.2352480314386386	No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC	14060	0.2092615033548816	No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG	14027	0.2087703490440202	No Hit
TAAAGAAACCCACAGAAACACACACACATACAATTAGCAAATGAATTCAA	13978	0.20804105930971087	No Hit
AAAAATAATAAACCGTTCATTTCTCAGGATGTGGTCATAGAATAAAGTTA	13895	0.20680573180057465	No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG	12574	0.18714467590215372	No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA	12387	0.18436146814060586	No Hit
CATGGGGTTGTGCCAATTAATTACCAAACATTGAGCCTGCAGGCTTTGAG	12168	0.1811019895321621	No Hit
AAGACAGGGGAAAGGAAGAAAAGGGAGTGAAGAGGGAATGGGAGAGGGAA	12082	0.17982201163112943	No Hit
*	12040	0.17919690614457856	No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT	11614	0.17285655049527704	No Hit
GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG	11499	0.17114495213924494	No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA	11380	0.16937381992735082	No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG	11307	0.16828732705786958	No Hit
GATACAACATTCAAGACAGAGGAACTGGATGCCAAGCCAGACACAGACCC	11305	0.1682575601299386	No Hit
CATGTATTTTGAACCGGCACCCCTGTTACCACAGAGTGTGGGAGGAACTG	11192	0.16657572870183748	No Hit
CATGTAATTCAGGCAGTTATGGGAGCCCTAGAGGGCCTGAGAGTTGCTATTGGAC	11037	0.1642687917871855	No Hit
GTCCAATAGCAACTCTCAGGCCCTCTAGGGCTCCCATAACTGCCTGAATTACATG	10986	0.16350973512494518	No Hit
CATGTAGACTTACCTTTCCTCATAGAGCTATCCTGGTTAATAACAGGCCA	10774	0.16035444076425992	No Hit
ACACTTACACCAAGGAATCTCCCAGGCATTATCAAAGCTACAGGAGACAA	10753	0.16004188802098449	No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC	10037	0.14938532782168895	No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA	9986	0.14862627115944863	No Hit
CACTCAGTATGAGGAGCCGGCTGTCATCACATAGGGACAGGGCTCTCCCT	9807	0.14596213110962475	No Hit
CATGCCCTCCAGCGGCCCCGGGGACACCAGCAGCTCTGCTGCGGAGCGGG	9742	0.14499470595186748	No Hit
TAACTGTGGAAAAACTAAGTTGTCACCAGCGGTACCTCATAGCATAACTT	9635	0.14340217530755933	No Hit
AGCAACAAGAACAATACAAACCTCATTTCCTTTAGTCCTTCTGCCTCTAT	9592	0.142762186357043	No Hit
ACAGATTTTGGGGTTGTGTTGTCACCCAAGAGATTGTTGTTTGCCATACT	9170	0.13648136456360344	No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT	8522	0.12683687991396167	No Hit
TAATCTACCTGAAGTCAATGAATGCAATTTTTCACACACACACACACACA	8496	0.12644990985085877	No Hit
ACCTATGTTACACCATCTTCAGTGCCAGTCTTGGGCAAAATTGTGCAAGA	8476	0.12615224057154883	No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT	8448	0.12573550358051494	No Hit
TAAAACTGCATCAAGTCATGGGGCATGTGGAAGGTAGGGAGGCAAGATGA	8331	0.12399413829655183	No Hit
CATGGAATATATATTCTCCTGGCTGGTGCTTATGATTGCAAAACTTGGAT	8327	0.12393460444068984	No Hit
CACACTGTCATTAGTTGCAGCATCTCCTGTAGCAATTGGGCTAAATCTGT	8304	0.12359228476948342	No Hit
CATGACTATAAATATAAAGCAAATATGATTATGCTCACAAGCTCTGGAAT	7835	0.11661194016966553	No Hit
ACTTGAAAGCAAGGATAGAGAAACACTTTAGAGATATGTGGGGTTTTTTT	7681	0.11431988671897907	No Hit
TAAATATAGCCCCAAAATGGTTGCTATAATAATCCCCATTTCATACTGGG	7521	0.11193853248449963	No Hit
CATGGTCACTCTCCCCAAAATATTATATTTTTTCTATAAAAAGAAAAAAA	7518	0.11189388209260312	No Hit
GTTTTTATACACTAAGGACACTCCCAGTGATGAAAATGAATTCCCCTCCA	7512	0.11180458130881014	No Hit
CACCAAAAACCCCAAGACAGAAATCTTAGGTATTCAGTTTCTTTTTCACA	7468	0.1111497088943283	No Hit
CTTGGGGGAGTGTGCCTCCAGCAGAGAAGTAATTCTGGCCTGGCAACCTA	7292	0.10853021923640091	No Hit
AGGACAACAGCTGATCCATACATTTGCTGACAGGTGTACCTGCGTTTCCC	7284	0.10841115152467692	No Hit
TGAGATTTTTTTGTGTATGTTTTTGACTCTTTTGAGTGGTAATCATATGT	7259	0.10803906492553952	No Hit
AAGTCCTCCTTATTCAAGATTTTGAAATTCTTAGCCTGGGAGTGCTGGAG	7238	0.10772651218226409	No Hit
GCACGTGGGAGAGTAAGCAGGCCAGGTAGAGCTGCAGAAAACTAAGGCCC	7237	0.10771162871829859	No Hit
TAACACTGATATACAAATTGGGACTCTTCATTCTGGAGAAAGCATCAGGT	7229	0.1075925610065746	No Hit
GACAACTTGGCCACCGACAGCTGAAGGGCTTTTCACCTGTTGACACAATTGCCAGTCA	7075	0.10530050755588816	No Hit
TGACTGGCAATTGTGTCAACAGGTGAAAAGCCCTTCAGCTGTCGGTGGCCAAGTTGTC	7017	0.10443726664588936	No Hit
TAATCCAGAGCAAGGCCTTGACTCCCTTCAGTTCTATGAAGAGAATGAGG	6895	0.10262148404209877	No Hit
AACTTTATAAACCAACTTCTGCTAGCTTCCAACTTCTCTTCTGCAGCTTC	6893	0.10259171711416777	No Hit
AAACCTGGAGTTTCTTATGTTGTCCCAACCAAGGCCGACAAAAGGAGATC	6881	0.1024131155465818	No Hit
CATGATATACATACTCTCTGTAAAGTTACTCTTGGTTGCTTGATAGGTAG	6857	0.1020559124114099	No Hit
TAGACATCTATAACTAACAACCACTTTTCTTACTATCATTGAAGTCAATA	6753	0.10050803215899826	No Hit
TGAGTAGGGGGAGCAAATCGTGCCTTGTCATTTTATTTGGAGGTTTCCTG	6739	0.10029966366348131	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	3910605	3.417261	6.2433496	2
TTTTT	3626290	3.406452	4.4344883	60-64
GGGGG	920150	3.1184406	8.182543	4
GGAGG	1080225	2.79167	5.181481	4
CCAGG	1067765	2.6754277	5.4659905	5
CCAGC	1074570	2.6511607	8.905594	5
CCTGG	1021695	2.5972865	5.484344	9
CCCCA	1061905	2.5797095	5.2985954	1
CCTCC	1046110	2.5783603	8.878137	6
CAGCC	1023665	2.5255687	6.286623	6
TGGGG	920140	2.4125977	11.783188	3
GGCAG	889745	2.2641191	5.219991	8
GCTGG	871390	2.2497144	10.702341	7
GAGGG	841910	2.1757827	7.0479183	9
GGGGA	822335	2.1251945	5.6615934	7
CCACC	872185	2.1188185	5.2726717	1
CTCCT	1029450	1.9936086	5.4019985	4
AAGAA	1706385	1.9554206	5.039614	3
GCCAG	775795	1.9438579	7.9465823	4
CACCA	1025765	1.9298354	6.0460014	8
TGGCT	961460	1.9204289	6.333557	3
CCCAC	788755	1.9161403	5.884513	9
TCCAG	987290	1.9138788	6.148533	7
TGGTG	933055	1.8927379	7.5364757	9
TGTGT	1195720	1.8765705	5.1239905	1
TGCAG	941095	1.8527609	6.0385847	3
TGTTT	1520435	1.8461009	5.8069816	3
TCCTC	927015	1.7952354	6.320705	5
GGCTC	706000	1.7947475	6.948222	4
AGGGG	675240	1.7450505	7.4919186	6
GAAAG	1072640	1.6119295	5.136686	5
GGCCC	496570	1.6066111	5.4355593	1
GATGG	767355	1.534258	6.2004595	1
TGCCC	610905	1.5291711	11.060555	3
GCTCC	609820	1.5264553	6.971177	2
CTGGT	762315	1.5226548	6.78374	8
TAAAA	1688915	1.4973491	7.9417357	1
ATGGA	976450	1.4887549	10.800505	2
CATGG	739055	1.454999	42.935383	1
GCAGT	679425	1.3376037	5.7780743	4
GGGGT	502390	1.3172616	5.4385333	4
ATGTG	839760	1.299001	7.2631	2
TGCTC	650730	1.2798281	5.379229	1
TGGGT	630420	1.278831	6.15133	3
ATGGG	630275	1.2601788	15.223597	2
ATGGC	629080	1.238488	11.097393	2
TGAGA	808700	1.2329929	7.3391643	1
AGACA	830580	1.2290154	5.307321	2
ATGAA	1048895	1.2194844	9.136273	2
ATGCC	616215	1.1945436	11.429264	2
GCACC	467300	1.1529145	6.6410017	8
GAGAC	584585	1.134364	7.64186	2
CATGT	718375	1.0941813	33.686783	1
AACAC	748255	1.0902078	5.050689	2
GTGTC	530335	1.0592959	7.191999	2
CATGA	702435	1.0545402	30.407196	1
ATGTT	878880	1.051807	8.26712	2
TAAAG	891490	1.0364795	6.8393464	1
ATGCT	660380	1.0058472	7.876425	2
ATGGT	636510	0.98459923	7.4749465	2
AGACC	510195	0.97482085	7.1658454	3
ATGAC	641525	0.9630982	7.7675114	2
ATGTA	809250	0.95457065	10.058474	2
CACTC	499885	0.9541653	5.142694	1
ATGAT	801330	0.9452284	6.703275	2
CATGC	474950	0.9206989	32.845356	1
CGCTG	341640	0.868495	8.6572895	6
GCGCC	267105	0.864196	5.330282	8
TAACT	649665	0.7545684	5.740721	1
ATGCA	502205	0.7539422	9.308469	2
TAAGT	632335	0.7458862	6.662057	1
TAAAC	608365	0.6964537	5.494168	1
TAAGG	437255	0.6666655	5.2792473	1
CGCAC	237175	0.5851541	5.864704	7
TGCCG	227915	0.57939065	7.2622313	5
CCGCA	225050	0.5552395	6.1180778	6
GACCG	211770	0.530618	6.836809	4
CCGTC	175500	0.4392983	5.2789316	7
TCGCT	220580	0.43382734	6.265655	5
ACCGC	174445	0.4303877	6.8291125	5
GCCGT	166445	0.42312568	5.300936	6
GTCGC	143325	0.36435148	8.055258	4
TGTCG	181720	0.36296916	6.465371	3
>>END_MODULE