FastQCFastQC Report
Wed 20 Mar 2013
8016-8-17.h.sapiens.aligned.bam

Summary

[OK] Basic Statistics

Measure Value
Filename 8016-8-17.h.sapiens.aligned.bam
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 6718866
Filtered Sequences 0
Sequence length 1-100
%GC 43

[WARN] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
TGAGACCGCACCAGCGACACA 21158 0.31490433058197614 No Hit
TGTGTCGCTGGTGCGGTCTCA 20812 0.30975465204991437 No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA 15943 0.23728706600191163 No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC 15806 0.2352480314386386 No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC 14060 0.2092615033548816 No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG 14027 0.2087703490440202 No Hit
TAAAGAAACCCACAGAAACACACACACATACAATTAGCAAATGAATTCAA 13978 0.20804105930971087 No Hit
AAAAATAATAAACCGTTCATTTCTCAGGATGTGGTCATAGAATAAAGTTA 13895 0.20680573180057465 No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG 12574 0.18714467590215372 No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA 12387 0.18436146814060586 No Hit
CATGGGGTTGTGCCAATTAATTACCAAACATTGAGCCTGCAGGCTTTGAG 12168 0.1811019895321621 No Hit
AAGACAGGGGAAAGGAAGAAAAGGGAGTGAAGAGGGAATGGGAGAGGGAA 12082 0.17982201163112943 No Hit
* 12040 0.17919690614457856 No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT 11614 0.17285655049527704 No Hit
GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG 11499 0.17114495213924494 No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA 11380 0.16937381992735082 No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG 11307 0.16828732705786958 No Hit
GATACAACATTCAAGACAGAGGAACTGGATGCCAAGCCAGACACAGACCC 11305 0.1682575601299386 No Hit
CATGTATTTTGAACCGGCACCCCTGTTACCACAGAGTGTGGGAGGAACTG 11192 0.16657572870183748 No Hit
CATGTAATTCAGGCAGTTATGGGAGCCCTAGAGGGCCTGAGAGTTGCTATTGGAC 11037 0.1642687917871855 No Hit
GTCCAATAGCAACTCTCAGGCCCTCTAGGGCTCCCATAACTGCCTGAATTACATG 10986 0.16350973512494518 No Hit
CATGTAGACTTACCTTTCCTCATAGAGCTATCCTGGTTAATAACAGGCCA 10774 0.16035444076425992 No Hit
ACACTTACACCAAGGAATCTCCCAGGCATTATCAAAGCTACAGGAGACAA 10753 0.16004188802098449 No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC 10037 0.14938532782168895 No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA 9986 0.14862627115944863 No Hit
CACTCAGTATGAGGAGCCGGCTGTCATCACATAGGGACAGGGCTCTCCCT 9807 0.14596213110962475 No Hit
CATGCCCTCCAGCGGCCCCGGGGACACCAGCAGCTCTGCTGCGGAGCGGG 9742 0.14499470595186748 No Hit
TAACTGTGGAAAAACTAAGTTGTCACCAGCGGTACCTCATAGCATAACTT 9635 0.14340217530755933 No Hit
AGCAACAAGAACAATACAAACCTCATTTCCTTTAGTCCTTCTGCCTCTAT 9592 0.142762186357043 No Hit
ACAGATTTTGGGGTTGTGTTGTCACCCAAGAGATTGTTGTTTGCCATACT 9170 0.13648136456360344 No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT 8522 0.12683687991396167 No Hit
TAATCTACCTGAAGTCAATGAATGCAATTTTTCACACACACACACACACA 8496 0.12644990985085877 No Hit
ACCTATGTTACACCATCTTCAGTGCCAGTCTTGGGCAAAATTGTGCAAGA 8476 0.12615224057154883 No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT 8448 0.12573550358051494 No Hit
TAAAACTGCATCAAGTCATGGGGCATGTGGAAGGTAGGGAGGCAAGATGA 8331 0.12399413829655183 No Hit
CATGGAATATATATTCTCCTGGCTGGTGCTTATGATTGCAAAACTTGGAT 8327 0.12393460444068984 No Hit
CACACTGTCATTAGTTGCAGCATCTCCTGTAGCAATTGGGCTAAATCTGT 8304 0.12359228476948342 No Hit
CATGACTATAAATATAAAGCAAATATGATTATGCTCACAAGCTCTGGAAT 7835 0.11661194016966553 No Hit
ACTTGAAAGCAAGGATAGAGAAACACTTTAGAGATATGTGGGGTTTTTTT 7681 0.11431988671897907 No Hit
TAAATATAGCCCCAAAATGGTTGCTATAATAATCCCCATTTCATACTGGG 7521 0.11193853248449963 No Hit
CATGGTCACTCTCCCCAAAATATTATATTTTTTCTATAAAAAGAAAAAAA 7518 0.11189388209260312 No Hit
GTTTTTATACACTAAGGACACTCCCAGTGATGAAAATGAATTCCCCTCCA 7512 0.11180458130881014 No Hit
CACCAAAAACCCCAAGACAGAAATCTTAGGTATTCAGTTTCTTTTTCACA 7468 0.1111497088943283 No Hit
CTTGGGGGAGTGTGCCTCCAGCAGAGAAGTAATTCTGGCCTGGCAACCTA 7292 0.10853021923640091 No Hit
AGGACAACAGCTGATCCATACATTTGCTGACAGGTGTACCTGCGTTTCCC 7284 0.10841115152467692 No Hit
TGAGATTTTTTTGTGTATGTTTTTGACTCTTTTGAGTGGTAATCATATGT 7259 0.10803906492553952 No Hit
AAGTCCTCCTTATTCAAGATTTTGAAATTCTTAGCCTGGGAGTGCTGGAG 7238 0.10772651218226409 No Hit
GCACGTGGGAGAGTAAGCAGGCCAGGTAGAGCTGCAGAAAACTAAGGCCC 7237 0.10771162871829859 No Hit
TAACACTGATATACAAATTGGGACTCTTCATTCTGGAGAAAGCATCAGGT 7229 0.1075925610065746 No Hit
GACAACTTGGCCACCGACAGCTGAAGGGCTTTTCACCTGTTGACACAATTGCCAGTCA 7075 0.10530050755588816 No Hit
TGACTGGCAATTGTGTCAACAGGTGAAAAGCCCTTCAGCTGTCGGTGGCCAAGTTGTC 7017 0.10443726664588936 No Hit
TAATCCAGAGCAAGGCCTTGACTCCCTTCAGTTCTATGAAGAGAATGAGG 6895 0.10262148404209877 No Hit
AACTTTATAAACCAACTTCTGCTAGCTTCCAACTTCTCTTCTGCAGCTTC 6893 0.10259171711416777 No Hit
AAACCTGGAGTTTCTTATGTTGTCCCAACCAAGGCCGACAAAAGGAGATC 6881 0.1024131155465818 No Hit
CATGATATACATACTCTCTGTAAAGTTACTCTTGGTTGCTTGATAGGTAG 6857 0.1020559124114099 No Hit
TAGACATCTATAACTAACAACCACTTTTCTTACTATCATTGAAGTCAATA 6753 0.10050803215899826 No Hit
TGAGTAGGGGGAGCAAATCGTGCCTTGTCATTTTATTTGGAGGTTTCCTG 6739 0.10029966366348131 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 3910605 3.417261 6.2433496 2
TTTTT 3626290 3.406452 4.4344883 60-64
GGGGG 920150 3.1184406 8.182543 4
GGAGG 1080225 2.79167 5.181481 4
CCAGG 1067765 2.6754277 5.4659905 5
CCAGC 1074570 2.6511607 8.905594 5
CCTGG 1021695 2.5972865 5.484344 9
CCCCA 1061905 2.5797095 5.2985954 1
CCTCC 1046110 2.5783603 8.878137 6
CAGCC 1023665 2.5255687 6.286623 6
TGGGG 920140 2.4125977 11.783188 3
GGCAG 889745 2.2641191 5.219991 8
GCTGG 871390 2.2497144 10.702341 7
GAGGG 841910 2.1757827 7.0479183 9
GGGGA 822335 2.1251945 5.6615934 7
CCACC 872185 2.1188185 5.2726717 1
CTCCT 1029450 1.9936086 5.4019985 4
AAGAA 1706385 1.9554206 5.039614 3
GCCAG 775795 1.9438579 7.9465823 4
CACCA 1025765 1.9298354 6.0460014 8
TGGCT 961460 1.9204289 6.333557 3
CCCAC 788755 1.9161403 5.884513 9
TCCAG 987290 1.9138788 6.148533 7
TGGTG 933055 1.8927379 7.5364757 9
TGTGT 1195720 1.8765705 5.1239905 1
TGCAG 941095 1.8527609 6.0385847 3
TGTTT 1520435 1.8461009 5.8069816 3
TCCTC 927015 1.7952354 6.320705 5
GGCTC 706000 1.7947475 6.948222 4
AGGGG 675240 1.7450505 7.4919186 6
GAAAG 1072640 1.6119295 5.136686 5
GGCCC 496570 1.6066111 5.4355593 1
GATGG 767355 1.534258 6.2004595 1
TGCCC 610905 1.5291711 11.060555 3
GCTCC 609820 1.5264553 6.971177 2
CTGGT 762315 1.5226548 6.78374 8
TAAAA 1688915 1.4973491 7.9417357 1
ATGGA 976450 1.4887549 10.800505 2
CATGG 739055 1.454999 42.935383 1
GCAGT 679425 1.3376037 5.7780743 4
GGGGT 502390 1.3172616 5.4385333 4
ATGTG 839760 1.299001 7.2631 2
TGCTC 650730 1.2798281 5.379229 1
TGGGT 630420 1.278831 6.15133 3
ATGGG 630275 1.2601788 15.223597 2
ATGGC 629080 1.238488 11.097393 2
TGAGA 808700 1.2329929 7.3391643 1
AGACA 830580 1.2290154 5.307321 2
ATGAA 1048895 1.2194844 9.136273 2
ATGCC 616215 1.1945436 11.429264 2
GCACC 467300 1.1529145 6.6410017 8
GAGAC 584585 1.134364 7.64186 2
CATGT 718375 1.0941813 33.686783 1
AACAC 748255 1.0902078 5.050689 2
GTGTC 530335 1.0592959 7.191999 2
CATGA 702435 1.0545402 30.407196 1
ATGTT 878880 1.051807 8.26712 2
TAAAG 891490 1.0364795 6.8393464 1
ATGCT 660380 1.0058472 7.876425 2
ATGGT 636510 0.98459923 7.4749465 2
AGACC 510195 0.97482085 7.1658454 3
ATGAC 641525 0.9630982 7.7675114 2
ATGTA 809250 0.95457065 10.058474 2
CACTC 499885 0.9541653 5.142694 1
ATGAT 801330 0.9452284 6.703275 2
CATGC 474950 0.9206989 32.845356 1
CGCTG 341640 0.868495 8.6572895 6
GCGCC 267105 0.864196 5.330282 8
TAACT 649665 0.7545684 5.740721 1
ATGCA 502205 0.7539422 9.308469 2
TAAGT 632335 0.7458862 6.662057 1
TAAAC 608365 0.6964537 5.494168 1
TAAGG 437255 0.6666655 5.2792473 1
CGCAC 237175 0.5851541 5.864704 7
TGCCG 227915 0.57939065 7.2622313 5
CCGCA 225050 0.5552395 6.1180778 6
GACCG 211770 0.530618 6.836809 4
CCGTC 175500 0.4392983 5.2789316 7
TCGCT 220580 0.43382734 6.265655 5
ACCGC 174445 0.4303877 6.8291125 5
GCCGT 166445 0.42312568 5.300936 6
GTCGC 143325 0.36435148 8.055258 4
TGTCG 181720 0.36296916 6.465371 3
Produced by FastQC (version 0.10.0)