##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	8016-8-18.h.sapiens.aligned.bam	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	5860096	
Filtered Sequences	0	
Sequence length	1-100	
%GC	44	
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.970022334105106	24.0	24.0	31.0	23.0	31.0
2	31.018498801758277	31.0	31.0	32.0	29.0	33.0
3	32.66739705699916	33.0	33.0	34.0	29.0	35.0
4	33.885402801860664	34.0	33.0	36.0	29.0	37.0
5	35.418319868915034	36.0	35.0	37.0	33.0	37.0
6	35.73933926993964	36.0	36.0	38.0	33.0	38.0
7	35.76443422202662	37.0	35.0	37.0	34.0	37.0
8	35.60327812636252	36.0	36.0	37.0	32.0	38.0
9	35.63071271179494	37.0	36.0	37.0	31.0	38.0
10-14	35.99457545842461	37.0	36.0	37.4	32.4	38.0
15-19	36.980684214786656	38.0	37.2	38.4	32.6	39.4
20-24	36.60830688756626	37.4	36.0	38.8	32.8	39.8
25-29	36.26698924877148	37.6	36.2	38.0	32.8	38.2
30-34	36.10226060487681	37.6	35.6	38.2	32.4	38.6
35-39	36.12964771778071	37.8	36.4	38.0	31.6	38.6
40-44	36.05207239452723	37.4	35.8	38.4	32.2	38.8
45-49	36.01560339717263	37.6	35.8	38.2	32.0	38.6
50-54	36.21962470149065	37.6	36.2	38.2	33.0	39.0
55-59	35.93536686281531	37.6	35.8	38.2	32.4	38.6
60-64	35.93554228575287	37.6	35.6	38.2	32.6	38.6
65-69	35.73972875394032	37.4	36.0	38.2	31.4	38.6
70-74	35.77876397104341	37.4	36.0	38.4	31.6	39.0
75-79	35.49655543470252	37.4	35.4	37.6	31.6	38.4
80-84	35.60460888004181	37.0	35.8	38.2	31.0	38.6
85-89	35.31781867129244	37.2	35.4	37.6	31.0	38.2
90-94	35.33791219854278	37.0	35.0	38.2	30.8	38.4
95-99	35.159421654460665	37.4	35.2	37.6	30.8	38.2
100	34.0604515451652	36.0	33.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
1	3.0
2	30.0
3	164.0
4	0.0
5	17.0
6	54.0
7	204.0
8	284.0
9	7305.0
10	666.0
11	886.0
12	1644.0
13	3068.0
14	3760.0
15	4056.0
16	4329.0
17	5284.0
18	7030.0
19	8744.0
20	10339.0
21	10466.0
22	10704.0
23	12714.0
24	15925.0
25	21170.0
26	28517.0
27	38465.0
28	53112.0
29	70166.0
30	92067.0
31	120062.0
32	156810.0
33	210594.0
34	310970.0
35	557017.0
36	1661940.0
37	2431521.0
38	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.916865286824827	20.284960252611345	22.659668902218723	38.1385055583451
2	18.032133599435802	52.66952626001793	15.093420389102238	14.204919751444024
3	18.23350991114149	35.79846206425154	30.299777853725224	15.66825017088175
4	34.087470191414106	25.170580134809832	20.94959550556421	19.792354168211855
5	20.13638204747394	27.81993809827369	26.93677034322815	25.106909511024217
6	19.617469749772827	29.599480633495855	26.376191325977693	24.406858290753622
7	22.1418825525554	28.3188979732775	27.49187486974296	22.047344604424136
8	21.334627894624443	27.577013163730747	29.129853578136377	21.95850536350843
9	21.206755880816903	29.126445349317137	27.463108641534205	22.203690128331754
10-14	21.83951484233656	27.8430924536605	27.123045639289078	23.19434706471386
15-19	21.231899183785497	27.665823174145903	27.66942821368305	23.432849428385545
20-24	21.419155921176596	27.400837713337843	27.832258566078576	23.34774779940698
25-29	21.647348799895283	27.244183435238263	28.602240907598432	22.506226857268018
30-34	21.99729191175636	27.718896647159163	27.800179440956008	22.48363200012847
35-39	22.09263648844203	26.63483773853344	28.91145259691477	22.36107317610976
40-44	22.195595842124874	27.748442308232953	27.632157720241086	22.423804129401088
45-49	21.754018598512022	28.439792956333754	28.065229517646046	21.740958927508174
50-54	22.240988333523372	27.73443879245138	27.838786492632245	22.185786381393
55-59	21.75102373270336	28.558917647723753	27.60368272748275	22.086375892090143
60-64	22.170497230137297	28.04006036098795	28.302488351871396	21.486954057003356
65-69	22.250750662051978	28.8691946804951	27.974620339769345	20.90543431768358
70-74	22.016175095622913	28.430189148629992	27.705704915462476	21.84793084028462
75-79	22.543811784896178	28.38958764697448	27.706163610409323	21.360436957720022
80-84	21.795490585902495	28.397013433699286	27.837752500513062	21.969743479885153
85-89	21.9632741736937	28.13029525177187	28.175619708303156	21.73081086623127
90-94	21.84141517220367	27.95023024737986	28.226246998241955	21.982107582174514
95-99	22.056484044582866	27.16124951858591	28.47735644239357	22.30490999443765
100	23.220239608145686	27.34119906040138	27.024650593322193	22.413910738130742
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	57.055370845169925
2	32.23705335087983
3	33.901760082023245
4	53.879824359625964
5	45.24329155849816
6	44.02432804052645
7	44.18922715697954
8	43.293133258132876
9	43.410446009148664
10-14	45.03386190705042
15-19	44.664748612171046
20-24	44.76690372058358
25-29	44.1535756571633
30-34	44.48092391188483
35-39	44.45370966455179
40-44	44.619399971525965
45-49	43.49497752602019
50-54	44.42677471491637
55-59	43.8373996247935
60-64	43.65745128714066
65-69	43.156184979735556
70-74	43.86410593590753
75-79	43.904248742616204
80-84	43.76523406578765
85-89	43.69408503992497
90-94	43.82352275437819
95-99	44.36139403902052
100	45.63415034627643
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	7646.0
1	7644.5
2	7644.5
3	7645.5
4	7652.5
5	7662.5
6	7674.5
7	7684.5
8	7701.5
9	7725.5
10	7761.0
11	7797.0
12	7828.0
13	7879.5
14	7923.5
15	8820.5
16	9385.0
17	8807.0
18	8988.5
19	10167.0
20	11528.0
21	13507.0
22	18285.5
23	26806.5
24	45952.0
25	60705.0
26	69674.0
27	86854.0
28	91662.5
29	106728.5
30	138053.0
31	153677.0
32	159315.0
33	173988.5
34	195963.5
35	198367.0
36	187491.0
37	178739.0
38	203435.0
39	221756.5
40	222304.5
41	232208.0
42	220200.5
43	222518.0
44	230272.5
45	211053.0
46	189844.0
47	169482.0
48	159399.5
49	173329.5
50	182449.0
51	184293.5
52	156239.0
53	102796.0
54	90510.0
55	91812.0
56	72939.5
57	61965.0
58	81067.5
59	79916.5
60	89639.5
61	121179.0
62	111282.5
63	101749.0
64	87449.0
65	70862.0
66	69405.0
67	62190.0
68	46296.0
69	38846.0
70	35619.5
71	29904.0
72	35098.0
73	26517.0
74	12812.5
75	16178.5
76	18150.5
77	17708.5
78	15703.0
79	14401.5
80	12593.5
81	10618.0
82	9306.5
83	8995.0
84	8747.5
85	7120.5
86	9360.0
87	9015.0
88	6400.0
89	6280.5
90	6186.5
91	6217.5
92	4188.0
93	2140.5
94	2092.5
95	2077.0
96	2069.0
97	2057.5
98	2058.0
99	2058.5
100	2058.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.034931168363112136
2	0.007586728351552588
3	0.002084713886689307
4	0.00674994792287014
5	0.00447750583101141
6	8.888499922054693E-4
7	3.764392813705675E-4
8	2.0534063615898019E-4
9	5.133541378653605E-5
10-14	0.001403206731230604
15-19	0.016625608681436223
20-24	0.015370326146881812
25-29	0.008951538281039008
30-34	0.008506178676401223
35-39	0.006135398409643793
40-44	0.012980451751608849
45-49	0.012398904608468856
50-54	0.011241000152501741
55-59	0.009746130479441957
60-64	0.04095040918546673
65-69	0.036166696814463356
70-74	0.02325902530632011
75-79	0.0525670299068994
80-84	0.04894927695096162
85-89	0.011822848882226743
90-94	0.0014407280283947749
95-99	0.009464570943767204
100	0.009408596608482056
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
0-1	7771.0
2-3	428.0
4-5	1641.0
6-7	6308.0
8-9	56.0
10-11	156.0
12-13	81.0
14-15	299.0
16-17	185.0
18-19	4073.0
20-21	51831.0
22-23	2721.0
24-25	1787.0
26-27	956.0
28-29	1140.0
30-31	1519.0
32-33	994.0
34-35	1221.0
36-37	1224.0
38-39	1539.0
40-41	5057.0
42-43	2292.0
44-45	992.0
46-47	1520.0
48-49	1375.0
50-51	20831.0
52-53	2718.0
54-55	22939.0
56-57	16135.0
58-59	47038.0
60-61	2083.0
62-63	11469.0
64-65	6583.0
66-67	8413.0
68-69	6132.0
70-71	27112.0
72-73	23048.0
74-75	15179.0
76-77	19041.0
78-79	7125.0
80-81	13627.0
82-83	14888.0
84-85	9932.0
86-87	3593.0
88-89	7121.0
90-91	12401.0
92-93	1681.0
94-95	4279.0
96-97	124016.0
98-99	0.0
100-101	5335546.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	97.34758960052667
#Duplication Level	Relative count
1	100.0
2	6.99594037302246
3	2.204165138677115
4	1.0834250127064395
5	0.8582476050774288
6	0.7134907001730649
7	0.7147774282166592
8	0.6993366916935271
9	0.7353650769141689
10++	12.526297504390959
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TGAGACCGCACCAGCGACACA	18407	0.31410748219824386	No Hit
TGTGTCGCTGGTGCGGTCTCA	18185	0.31031914835524876	No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC	12077	0.20608877397230355	No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG	11946	0.20385331571359924	No Hit
AAAAATAATAAACCGTTCATTTCTCAGGATGTGGTCATAGAATAAAGTTA	11009	0.18786381656546242	No Hit
TAAAGAAACCCACAGAAACACACACACATACAATTAGCAAATGAATTCAA	10964	0.1870959110567472	No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA	10508	0.179314468568433	No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC	10462	0.17852949849285746	No Hit
CACTCAGTATGAGGAGCCGGCTGTCATCACATAGGGACAGGGCTCTCCCT	9915	0.1691951804202525	No Hit
CATGCCCTCCAGCGGCCCCGGGGACACCAGCAGCTCTGCTGCGGAGCGGG	9773	0.16677201192608448	No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT	9369	0.1598779269145079	No Hit
GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG	9292	0.15856395526626185	No Hit
TAACTGTGGAAAAACTAAGTTGTCACCAGCGGTACCTCATAGCATAACTT	9144	0.1560383993709318	No Hit
AGCAACAAGAACAATACAAACCTCATTTCCTTTAGTCCTTCTGCCTCTAT	9127	0.15574830173430604	No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG	8958	0.15286438993490892	No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA	8819	0.15049241514132192	No Hit
CATGGGGTTGTGCCAATTAATTACCAAACATTGAGCCTGCAGGCTTTGAG	8716	0.1487347647547071	No Hit
CATGTATTTTGAACCGGCACCCCTGTTACCACAGAGTGTGGGAGGAACTG	8657	0.14772795530994715	No Hit
GATACAACATTCAAGACAGAGGAACTGGATGCCAAGCCAGACACAGACCC	8598	0.1467211458651872	No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA	8437	0.14397375060067275	No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG	8392	0.14320584509195752	No Hit
AAGACAGGGGAAAGGAAGAAAAGGGAGTGAAGAGGGAATGGGAGAGGGAA	8381	0.1430181348564938	No Hit
CATGTAGACTTACCTTTCCTCATAGAGCTATCCTGGTTAATAACAGGCCA	8363	0.14271097265300775	No Hit
ACACTTACACCAAGGAATCTCCCAGGCATTATCAAAGCTACAGGAGACAA	8313	0.14185774430999082	No Hit
AGGACAACAGCTGATCCATACATTTGCTGACAGGTGTACCTGCGTTTCCC	7989	0.13632882464724128	No Hit
CTTGGGGGAGTGTGCCTCCAGCAGAGAAGTAATTCTGGCCTGGCAACCTA	7959	0.13581688764143113	No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT	7714	0.1316360687606483	No Hit
GACGCGACCGGACCGAGCGCCAAACGCGGCAGCCCAATCGCCATCGCTTT	7670	0.13088522781879341	No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT	7568	0.1291446419990389	No Hit
CATGTAATTCAGGCAGTTATGGGAGCCCTAGAGGGCCTGAGAGTTGCTATTGGAC	7515	0.12824021995544102	No Hit
GTCCAATAGCAACTCTCAGGCCCTCTAGGGCTCCCATAACTGCCTGAATTACATG	7500	0.12798425145253595	No Hit
CGAGCCCGGCGGGCCCTGTGATTGGACGGGCGCCCGCCTCGCGTCCCGCC	7416	0.12655082783626753	No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA	7410	0.1264484404351055	No Hit
ACCTATGTTACACCATCTTCAGTGCCAGTCTTGGGCAAAATTGTGCAAGA	7389	0.1260900845310384	No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC	7339	0.1252368561880215	No Hit
TAAAACTGCATCAAGTCATGGGGCATGTGGAAGGTAGGGAGGCAAGATGA	7206	0.12296726879559652	No Hit
ATTGTTTCAAAAAAAATCAAACTGTAGTTGTTTTGGCGATAGGTCTCACG	7161	0.12219936328688132	No Hit
CATGGGCTCGGCCACGCGCTACCACACCTACCTGCCGCCGCCCTACCCCG	7158	0.1221481695863003	No Hit
GAGAACTGGTAGGAGCCGGCCGAGGCGCCGTAGTACAGGTGGTAGGAGGG	7146	0.12194339478397624	No Hit
ACAGATTTTGGGGTTGTGTTGTCACCCAAGAGATTGTTGTTTGCCATACT	6966	0.11887177274911537	No Hit
*	6847	0.11684108929273514	No Hit
CATGGCTCCACTCTCCACCGTCTCCACGCACACCTTCTCCAGCATCTGGG	6819	0.11636328142064567	No Hit
GAGGGAGAGGCCGGAGCTGCCCAGGACAGGCTCTGGGTCCTGCCCTTGCA	6797	0.11598786094971823	No Hit
CAGCTGGGTGGCCCCAGGAGAGGCGAGGCCCTGAGAGAAAGGCTTTCTAC	6670	0.11382066095845529	No Hit
TAATCTACCTGAAGTCAATGAATGCAATTTTTCACACACACACACACACA	6649	0.1134623050543882	No Hit
GCCTCAGCCTTCCTTCGCAGAAAGTTGAGCAGGTCGCCATAGCAACAGTA	6646	0.11341111135380717	No Hit
CATGGAGGGGAGCACTCAGCACCTCCCTCACCCCACACCCTTGGCTGCTC	6645	0.11339404678694684	No Hit
ATCCTTCACAAAGTCTTTGAGATATATTTTTATCAAATATTTAGCATGGATCCCGGTACACTTTCAATACTTA	6191	0.10564673343235333	No Hit
TAAGTATTGAAAGTGTACCGGGATCCATGCTAAATATTTGATAAAAATATATCTCAAAGACTTTGTGAAGGAT	6140	0.10477644052247607	No Hit
CATGACTATAAATATAAAGCAAATATGATTATGCTCACAAGCTCTGGAAT	6093	0.10397440588004019	No Hit
GACAACTTGGCCACCGACAGCTGAAGGGCTTTTCACCTGTTGACACAATTGCCAGTCA	6012	0.1025921759643528	No Hit
TGAGTAGGGGGAGCAAATCGTGCCTTGTCATTTTATTTGGAGGTTTCCTG	6012	0.1025921759643528	No Hit
ACTTGAAAGCAAGGATAGAGAAACACTTTAGAGATATGTGGGGTTTTTTT	6005	0.10247272399633045	No Hit
TGACTGGCAATTGTGTCAACAGGTGAAAAGCCCTTCAGCTGTCGGTGGCCAAGTTGTC	5989	0.10219969092656503	No Hit
CACACTGTCATTAGTTGCAGCATCTCCTGTAGCAATTGGGCTAAATCTGT	5900	0.10068094447599493	No Hit
CATGGAATATATATTCTCCTGGCTGGTGCTTATGATTGCAAAACTTGGAT	5866	0.10010074920274344	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	3378010	3.5011144	6.738438	2
TTTTT	3096005	3.4832587	4.4397492	60-64
GGGGG	860135	3.1296659	9.14846	4
GGAGG	967600	2.738735	5.2701163	4
CCAGG	973475	2.6741471	5.738365	5
CCAGC	984545	2.6643991	7.9939237	5
CCTCC	958975	2.598974	9.313325	6
CCTGG	927240	2.5892882	6.064039	9
CCCCA	962940	2.5672379	5.7643943	1
CAGCC	928380	2.512404	6.4328904	6
TGGGG	827855	2.3819695	12.117517	3
CTCCC	864950	2.3441513	5.0206623	3
GCTGG	789920	2.2390726	10.058051	7
GAGGG	750305	2.1236942	5.6066856	9
GGGGA	735160	2.0808272	5.5655417	5
CTCCT	917435	1.9958014	5.540647	4
AAGAA	1486580	1.9806643	5.264945	2
CTCCA	924125	1.9776298	5.0959215	6
TCCAG	891115	1.9357302	5.7230334	4
CCCAC	720335	1.9204428	5.7161465	9
GCCAG	694030	1.9065087	7.5588017	4
TGGCT	843865	1.8915099	6.6273694	3
CACCA	898480	1.8914504	5.3813725	9
TGTTT	1319385	1.8771765	5.78559	3
TGCAG	849815	1.873839	6.138045	3
TGTGT	1040515	1.87211	5.4341836	1
TGGTG	814515	1.8532395	7.279263	9
GCCCA	679490	1.8388519	5.335381	4
GGCTC	653815	1.8257576	8.33134	1
TCCTC	833860	1.8139911	6.389447	5
AAACA	1350885	1.7731446	5.0877185	2
AGGGG	603140	1.7071524	6.9841995	6
GGCCC	478865	1.6659194	5.46355	1
GCTCC	568505	1.5639604	8.998582	2
TGCCC	564160	1.5520073	11.634469	3
TAAAA	1459015	1.5372087	8.67822	1
GATGG	681245	1.524783	5.60165	1
CTGGT	673725	1.510144	6.559491	8
ATGGA	847005	1.4747332	10.1041	2
CATGG	656935	1.4485394	41.94843	1
GCAGT	604295	1.3324684	6.175328	4
ATGTG	730395	1.2927456	7.6164923	2
TGCTC	581195	1.2833961	6.047052	1
TGGGT	558675	1.2711351	5.9549117	3
ATGGG	559165	1.25154	15.16701	2
GGCAC	452805	1.2438606	5.0487375	1
ATGGC	557270	1.2287784	11.0694895	2
ATGAA	905700	1.2266896	8.310436	2
TGAGA	697735	1.2148371	7.19655	1
ATGCC	541115	1.1754405	11.674164	2
GCACC	416895	1.1282113	6.2361712	8
GAGAC	517255	1.1219794	7.76912	2
CATGT	634675	1.1066489	32.8694	1
AACAC	650625	1.0815258	5.0292945	2
ATGTT	762710	1.0674936	8.308706	2
GTGTC	469980	1.0534526	7.031218	2
CATGA	612000	1.0497409	28.483437	1
TAAAG	757505	1.0259727	6.8248363	1
ATGCT	574450	1.0016378	7.608992	2
CACTC	460350	0.98515016	5.319923	1
ATGGT	555000	0.9823093	7.3600807	2
AGACC	453695	0.9694989	7.0963187	3
ATGAC	563725	0.9669365	7.04887	2
ATGAT	698060	0.961105	6.489892	2
ATGTA	685270	0.9434954	9.4131975	2
CATGC	426135	0.92567444	31.8086	1
GCGCC	261160	0.90854734	5.3952885	8
CGCTG	309125	0.8632218	8.337089	6
TAAGA	599915	0.8125312	5.4221354	1
ATGCA	445180	0.76360077	8.996859	2
TAAGT	552135	0.7601921	6.7280226	1
TAACT	549855	0.7458121	6.0410776	1
TAAAC	512115	0.68331504	5.6872506	1
TAAGG	376215	0.65503365	5.682127	1
CGCAC	219780	0.59477395	5.6871595	7
TGCCG	202035	0.5641764	6.7551956	5
CCGCA	208365	0.5638823	5.966007	6
GACCG	193830	0.5324533	6.87222	4
TCGCT	198185	0.43763256	6.0224075	5
ACCGC	157395	0.42594615	6.9541464	5
GTCGC	130955	0.36568773	7.538043	4
TGTCG	156635	0.35109484	6.189447	3
>>END_MODULE