FastQCFastQC Report
Wed 20 Mar 2013
8016-8-18.h.sapiens.aligned.bam

Summary

[OK] Basic Statistics

Measure Value
Filename 8016-8-18.h.sapiens.aligned.bam
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 5860096
Filtered Sequences 0
Sequence length 1-100
%GC 44

[WARN] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
TGAGACCGCACCAGCGACACA 18407 0.31410748219824386 No Hit
TGTGTCGCTGGTGCGGTCTCA 18185 0.31031914835524876 No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC 12077 0.20608877397230355 No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG 11946 0.20385331571359924 No Hit
AAAAATAATAAACCGTTCATTTCTCAGGATGTGGTCATAGAATAAAGTTA 11009 0.18786381656546242 No Hit
TAAAGAAACCCACAGAAACACACACACATACAATTAGCAAATGAATTCAA 10964 0.1870959110567472 No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA 10508 0.179314468568433 No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC 10462 0.17852949849285746 No Hit
CACTCAGTATGAGGAGCCGGCTGTCATCACATAGGGACAGGGCTCTCCCT 9915 0.1691951804202525 No Hit
CATGCCCTCCAGCGGCCCCGGGGACACCAGCAGCTCTGCTGCGGAGCGGG 9773 0.16677201192608448 No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT 9369 0.1598779269145079 No Hit
GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG 9292 0.15856395526626185 No Hit
TAACTGTGGAAAAACTAAGTTGTCACCAGCGGTACCTCATAGCATAACTT 9144 0.1560383993709318 No Hit
AGCAACAAGAACAATACAAACCTCATTTCCTTTAGTCCTTCTGCCTCTAT 9127 0.15574830173430604 No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG 8958 0.15286438993490892 No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA 8819 0.15049241514132192 No Hit
CATGGGGTTGTGCCAATTAATTACCAAACATTGAGCCTGCAGGCTTTGAG 8716 0.1487347647547071 No Hit
CATGTATTTTGAACCGGCACCCCTGTTACCACAGAGTGTGGGAGGAACTG 8657 0.14772795530994715 No Hit
GATACAACATTCAAGACAGAGGAACTGGATGCCAAGCCAGACACAGACCC 8598 0.1467211458651872 No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA 8437 0.14397375060067275 No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG 8392 0.14320584509195752 No Hit
AAGACAGGGGAAAGGAAGAAAAGGGAGTGAAGAGGGAATGGGAGAGGGAA 8381 0.1430181348564938 No Hit
CATGTAGACTTACCTTTCCTCATAGAGCTATCCTGGTTAATAACAGGCCA 8363 0.14271097265300775 No Hit
ACACTTACACCAAGGAATCTCCCAGGCATTATCAAAGCTACAGGAGACAA 8313 0.14185774430999082 No Hit
AGGACAACAGCTGATCCATACATTTGCTGACAGGTGTACCTGCGTTTCCC 7989 0.13632882464724128 No Hit
CTTGGGGGAGTGTGCCTCCAGCAGAGAAGTAATTCTGGCCTGGCAACCTA 7959 0.13581688764143113 No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT 7714 0.1316360687606483 No Hit
GACGCGACCGGACCGAGCGCCAAACGCGGCAGCCCAATCGCCATCGCTTT 7670 0.13088522781879341 No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT 7568 0.1291446419990389 No Hit
CATGTAATTCAGGCAGTTATGGGAGCCCTAGAGGGCCTGAGAGTTGCTATTGGAC 7515 0.12824021995544102 No Hit
GTCCAATAGCAACTCTCAGGCCCTCTAGGGCTCCCATAACTGCCTGAATTACATG 7500 0.12798425145253595 No Hit
CGAGCCCGGCGGGCCCTGTGATTGGACGGGCGCCCGCCTCGCGTCCCGCC 7416 0.12655082783626753 No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA 7410 0.1264484404351055 No Hit
ACCTATGTTACACCATCTTCAGTGCCAGTCTTGGGCAAAATTGTGCAAGA 7389 0.1260900845310384 No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC 7339 0.1252368561880215 No Hit
TAAAACTGCATCAAGTCATGGGGCATGTGGAAGGTAGGGAGGCAAGATGA 7206 0.12296726879559652 No Hit
ATTGTTTCAAAAAAAATCAAACTGTAGTTGTTTTGGCGATAGGTCTCACG 7161 0.12219936328688132 No Hit
CATGGGCTCGGCCACGCGCTACCACACCTACCTGCCGCCGCCCTACCCCG 7158 0.1221481695863003 No Hit
GAGAACTGGTAGGAGCCGGCCGAGGCGCCGTAGTACAGGTGGTAGGAGGG 7146 0.12194339478397624 No Hit
ACAGATTTTGGGGTTGTGTTGTCACCCAAGAGATTGTTGTTTGCCATACT 6966 0.11887177274911537 No Hit
* 6847 0.11684108929273514 No Hit
CATGGCTCCACTCTCCACCGTCTCCACGCACACCTTCTCCAGCATCTGGG 6819 0.11636328142064567 No Hit
GAGGGAGAGGCCGGAGCTGCCCAGGACAGGCTCTGGGTCCTGCCCTTGCA 6797 0.11598786094971823 No Hit
CAGCTGGGTGGCCCCAGGAGAGGCGAGGCCCTGAGAGAAAGGCTTTCTAC 6670 0.11382066095845529 No Hit
TAATCTACCTGAAGTCAATGAATGCAATTTTTCACACACACACACACACA 6649 0.1134623050543882 No Hit
GCCTCAGCCTTCCTTCGCAGAAAGTTGAGCAGGTCGCCATAGCAACAGTA 6646 0.11341111135380717 No Hit
CATGGAGGGGAGCACTCAGCACCTCCCTCACCCCACACCCTTGGCTGCTC 6645 0.11339404678694684 No Hit
ATCCTTCACAAAGTCTTTGAGATATATTTTTATCAAATATTTAGCATGGATCCCGGTACACTTTCAATACTTA 6191 0.10564673343235333 No Hit
TAAGTATTGAAAGTGTACCGGGATCCATGCTAAATATTTGATAAAAATATATCTCAAAGACTTTGTGAAGGAT 6140 0.10477644052247607 No Hit
CATGACTATAAATATAAAGCAAATATGATTATGCTCACAAGCTCTGGAAT 6093 0.10397440588004019 No Hit
GACAACTTGGCCACCGACAGCTGAAGGGCTTTTCACCTGTTGACACAATTGCCAGTCA 6012 0.1025921759643528 No Hit
TGAGTAGGGGGAGCAAATCGTGCCTTGTCATTTTATTTGGAGGTTTCCTG 6012 0.1025921759643528 No Hit
ACTTGAAAGCAAGGATAGAGAAACACTTTAGAGATATGTGGGGTTTTTTT 6005 0.10247272399633045 No Hit
TGACTGGCAATTGTGTCAACAGGTGAAAAGCCCTTCAGCTGTCGGTGGCCAAGTTGTC 5989 0.10219969092656503 No Hit
CACACTGTCATTAGTTGCAGCATCTCCTGTAGCAATTGGGCTAAATCTGT 5900 0.10068094447599493 No Hit
CATGGAATATATATTCTCCTGGCTGGTGCTTATGATTGCAAAACTTGGAT 5866 0.10010074920274344 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 3378010 3.5011144 6.738438 2
TTTTT 3096005 3.4832587 4.4397492 60-64
GGGGG 860135 3.1296659 9.14846 4
GGAGG 967600 2.738735 5.2701163 4
CCAGG 973475 2.6741471 5.738365 5
CCAGC 984545 2.6643991 7.9939237 5
CCTCC 958975 2.598974 9.313325 6
CCTGG 927240 2.5892882 6.064039 9
CCCCA 962940 2.5672379 5.7643943 1
CAGCC 928380 2.512404 6.4328904 6
TGGGG 827855 2.3819695 12.117517 3
CTCCC 864950 2.3441513 5.0206623 3
GCTGG 789920 2.2390726 10.058051 7
GAGGG 750305 2.1236942 5.6066856 9
GGGGA 735160 2.0808272 5.5655417 5
CTCCT 917435 1.9958014 5.540647 4
AAGAA 1486580 1.9806643 5.264945 2
CTCCA 924125 1.9776298 5.0959215 6
TCCAG 891115 1.9357302 5.7230334 4
CCCAC 720335 1.9204428 5.7161465 9
GCCAG 694030 1.9065087 7.5588017 4
TGGCT 843865 1.8915099 6.6273694 3
CACCA 898480 1.8914504 5.3813725 9
TGTTT 1319385 1.8771765 5.78559 3
TGCAG 849815 1.873839 6.138045 3
TGTGT 1040515 1.87211 5.4341836 1
TGGTG 814515 1.8532395 7.279263 9
GCCCA 679490 1.8388519 5.335381 4
GGCTC 653815 1.8257576 8.33134 1
TCCTC 833860 1.8139911 6.389447 5
AAACA 1350885 1.7731446 5.0877185 2
AGGGG 603140 1.7071524 6.9841995 6
GGCCC 478865 1.6659194 5.46355 1
GCTCC 568505 1.5639604 8.998582 2
TGCCC 564160 1.5520073 11.634469 3
TAAAA 1459015 1.5372087 8.67822 1
GATGG 681245 1.524783 5.60165 1
CTGGT 673725 1.510144 6.559491 8
ATGGA 847005 1.4747332 10.1041 2
CATGG 656935 1.4485394 41.94843 1
GCAGT 604295 1.3324684 6.175328 4
ATGTG 730395 1.2927456 7.6164923 2
TGCTC 581195 1.2833961 6.047052 1
TGGGT 558675 1.2711351 5.9549117 3
ATGGG 559165 1.25154 15.16701 2
GGCAC 452805 1.2438606 5.0487375 1
ATGGC 557270 1.2287784 11.0694895 2
ATGAA 905700 1.2266896 8.310436 2
TGAGA 697735 1.2148371 7.19655 1
ATGCC 541115 1.1754405 11.674164 2
GCACC 416895 1.1282113 6.2361712 8
GAGAC 517255 1.1219794 7.76912 2
CATGT 634675 1.1066489 32.8694 1
AACAC 650625 1.0815258 5.0292945 2
ATGTT 762710 1.0674936 8.308706 2
GTGTC 469980 1.0534526 7.031218 2
CATGA 612000 1.0497409 28.483437 1
TAAAG 757505 1.0259727 6.8248363 1
ATGCT 574450 1.0016378 7.608992 2
CACTC 460350 0.98515016 5.319923 1
ATGGT 555000 0.9823093 7.3600807 2
AGACC 453695 0.9694989 7.0963187 3
ATGAC 563725 0.9669365 7.04887 2
ATGAT 698060 0.961105 6.489892 2
ATGTA 685270 0.9434954 9.4131975 2
CATGC 426135 0.92567444 31.8086 1
GCGCC 261160 0.90854734 5.3952885 8
CGCTG 309125 0.8632218 8.337089 6
TAAGA 599915 0.8125312 5.4221354 1
ATGCA 445180 0.76360077 8.996859 2
TAAGT 552135 0.7601921 6.7280226 1
TAACT 549855 0.7458121 6.0410776 1
TAAAC 512115 0.68331504 5.6872506 1
TAAGG 376215 0.65503365 5.682127 1
CGCAC 219780 0.59477395 5.6871595 7
TGCCG 202035 0.5641764 6.7551956 5
CCGCA 208365 0.5638823 5.966007 6
GACCG 193830 0.5324533 6.87222 4
TCGCT 198185 0.43763256 6.0224075 5
ACCGC 157395 0.42594615 6.9541464 5
GTCGC 130955 0.36568773 7.538043 4
TGTCG 156635 0.35109484 6.189447 3
Produced by FastQC (version 0.10.0)