##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	8016-8-19.h.sapiens.aligned.bam	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	6065658	
Filtered Sequences	0	
Sequence length	1-100	
%GC	43	
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.94181571067805	24.0	24.0	31.0	23.0	31.0
2	31.006639178520594	31.0	31.0	32.0	27.0	33.0
3	32.65177484120225	33.0	33.0	34.0	29.0	35.0
4	33.8617087110344	34.0	33.0	36.0	29.0	37.0
5	35.39684368068919	36.0	35.0	37.0	33.0	37.0
6	35.72258067292014	36.0	36.0	38.0	33.0	38.0
7	35.74110915936085	37.0	35.0	37.0	34.0	37.0
8	35.59166771267641	36.0	36.0	37.0	32.0	38.0
9	35.611804275410464	37.0	36.0	37.0	31.0	38.0
10-14	35.97438770491494	37.0	36.0	37.4	32.4	38.0
15-19	36.96196442152139	38.0	37.2	38.4	32.6	39.4
20-24	36.59923055723636	37.4	36.0	38.8	32.8	39.8
25-29	36.25449063857485	37.6	36.2	38.0	32.8	38.2
30-34	36.09599829578685	37.6	35.6	38.2	32.4	38.6
35-39	36.12532436014359	37.8	36.4	38.0	31.6	38.6
40-44	36.05769930413634	37.4	36.0	38.4	32.4	38.8
45-49	36.01264058222152	37.6	35.8	38.2	32.0	38.6
50-54	36.22200657902219	37.6	36.2	38.2	33.0	39.0
55-59	35.94038581720649	37.6	35.8	38.2	32.4	38.6
60-64	35.95697236794133	37.6	35.6	38.2	32.6	38.6
65-69	35.762547908848795	37.4	36.0	38.2	31.4	38.6
70-74	35.801469913939584	37.4	36.0	38.4	31.6	39.0
75-79	35.516152266563246	37.4	35.4	37.6	31.6	38.4
80-84	35.62266404586984	37.2	35.8	38.2	31.0	38.6
85-89	35.334989881340746	37.2	35.4	37.6	31.0	38.2
90-94	35.35816348712278	37.0	35.0	38.2	30.8	38.4
95-99	35.18511955222911	37.4	35.2	37.6	30.8	38.2
100	34.10172547451046	36.0	33.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	1.0
1	9.0
2	13.0
3	81.0
4	0.0
5	9.0
6	43.0
7	178.0
8	350.0
9	11842.0
10	745.0
11	1064.0
12	1607.0
13	2612.0
14	3426.0
15	4162.0
16	4918.0
17	5815.0
18	7143.0
19	8614.0
20	10268.0
21	11020.0
22	11877.0
23	13648.0
24	16684.0
25	22056.0
26	29508.0
27	39843.0
28	55015.0
29	72770.0
30	95323.0
31	123610.0
32	159664.0
33	214649.0
34	316093.0
35	570245.0
36	1727714.0
37	2523031.0
38	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.18531149222835	19.961484676004257	21.39269600327081	39.46050782849658
2	17.172965187113626	52.625805621346686	15.605294150395647	14.595935041144035
3	18.300900954775173	35.48497084398081	30.784028392324597	15.430099808919417
4	33.643860357505616	25.1495334081854	21.23461958608439	19.9719866482246
5	20.237793247966906	27.925335850426958	26.84283235210559	24.994038549500544
6	19.863029680598416	29.9996739905166	26.560075356181912	23.577220972703078
7	21.436930193232357	28.475166165783435	28.128651784840503	21.95925185614371
8	20.95987454481926	27.780672019818475	29.482265545695263	21.777187889667005
9	21.2818430136426	28.800366960019407	27.4918133637367	22.425976662601293
10-14	21.614309681610816	28.059576952487237	27.239052341352547	23.0870610245494
15-19	21.106690031627636	27.847308122678697	27.704748379386285	23.34125346630738
20-24	21.406763660023252	27.09939398813098	28.017460266296972	23.476382085548792
25-29	21.445317228995734	27.39837137081646	28.66718077897173	22.489130621216074
30-34	22.208832397384576	27.81683439672433	27.838519245828618	22.135813960062475
35-39	22.11608604401708	26.65933654868569	28.986540001338106	22.23803740595912
40-44	22.164686504020086	27.805275134987255	27.73359856558685	22.296439795405814
45-49	21.855608295049205	28.422415259586725	28.192707140854928	21.529269304509146
50-54	22.267787755857597	27.765951560388885	27.94303518365811	22.023225500095407
55-59	21.724942317103725	28.662740188316782	27.568743915303628	22.04357357927586
60-64	22.023918189061153	28.160885561494403	28.422873500675543	21.392322748768898
65-69	22.035211618376096	29.045433234602914	28.092463521804493	20.826891625216497
70-74	21.74072777496844	28.65454824609791	27.757176684293	21.847547294640645
75-79	22.344325532977575	28.673616158093353	27.777983603347078	21.204074705582
80-84	21.51568201686162	28.46521658308602	28.1269978170578	21.892103582994558
85-89	21.72344763007282	28.300474175461677	28.30857748731368	21.667500707151824
90-94	21.75366417287127	28.00383656133184	28.190539208559866	22.051960057237018
95-99	21.925196637979187	27.28498322402236	28.587225865683703	22.20259427231476
100	23.221241342776803	27.585170201283542	27.117784488012187	22.075803967927467
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	58.64581932072493
2	31.768900228257664
3	33.73100076369459
4	53.61584700573021
5	45.23183179746745
6	43.44025065330149
7	43.396182049376065
8	42.73706243448627
9	43.70781967624389
10-14	44.70137070616021
15-19	44.447943497935015
20-24	44.883145745572044
25-29	43.93444785021181
30-34	44.344646357447054
35-39	44.3541234499762
40-44	44.4611262994259
45-49	43.38487759955835
50-54	44.291013255953004
55-59	43.76851589637959
60-64	43.41624093783005
65-69	42.862103243592585
70-74	43.58827506960909
75-79	43.54840023855957
80-84	43.40778559985618
85-89	43.39094833722464
90-94	43.80562423010829
95-99	44.12779091029394
100	45.29704531070427
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	11777.0
1	11774.5
2	11772.0
3	11775.0
4	11783.0
5	11793.0
6	11809.0
7	11826.5
8	11846.0
9	11866.0
10	11897.0
11	11920.0
12	11929.5
13	12025.0
14	12096.5
15	12521.0
16	12804.5
17	12637.0
18	13061.5
19	14053.0
20	15722.0
21	17803.5
22	22350.0
23	29437.5
24	43210.0
25	57118.5
26	74944.5
27	94234.5
28	94633.5
29	110014.5
30	144693.5
31	161097.5
32	165929.5
33	184374.5
34	209768.5
35	207877.0
36	194523.5
37	186613.0
38	212169.5
39	233285.0
40	234329.0
41	258264.0
42	248076.5
43	246671.5
44	255187.5
45	235418.0
46	215182.5
47	186106.0
48	178470.0
49	194611.5
50	202880.5
51	206718.0
52	171473.0
53	112550.0
54	98144.5
55	93762.5
56	72479.5
57	63362.5
58	82308.0
59	79894.5
60	80203.0
61	98170.5
62	87783.0
63	74401.5
64	66164.5
65	62155.0
66	62360.5
67	57758.0
68	42280.5
69	34116.5
70	33161.5
71	28677.5
72	33978.5
73	26156.0
74	12908.5
75	15307.0
76	16313.0
77	15085.0
78	13348.5
79	13047.0
80	11196.0
81	9682.5
82	8977.5
83	9311.5
84	10511.0
85	10342.5
86	10165.0
87	8649.5
88	8032.5
89	8861.5
90	9731.0
91	10689.5
92	9445.0
93	8163.5
94	8106.5
95	8093.0
96	8087.5
97	8085.0
98	8073.5
99	8064.0
100	8064.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05310223556949633
2	0.008490589569029898
3	0.0023952641827722026
4	0.007599175852858809
5	0.004977274031345582
6	9.598156624374651E-4
7	4.139741786089938E-4
8	1.987142525479307E-4
9	1.1591683927236019E-4
10-14	0.001394320953938094
15-19	0.017895857832629113
20-24	0.01614375336252056
25-29	0.008105687238561199
30-34	0.007843266404126685
35-39	0.005078546122358193
40-44	0.012820646403700146
45-49	0.011312776885563106
50-54	0.010428220979032234
55-59	0.010819070153107239
60-64	0.05301872372946121
65-69	0.04258020787702
70-74	0.027020029023172683
75-79	0.07391717462977739
80-84	0.0632570649609652
85-89	0.014609818971348634
90-94	0.0071363289265739035
95-99	0.008874938108984239
100	0.007262569529618547
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
0-1	11897.0
2-3	473.0
4-5	10461.0
6-7	4005.0
8-9	11.0
10-11	20.0
12-13	64.0
14-15	77.0
16-17	260.0
18-19	2372.0
20-21	23116.0
22-23	2889.0
24-25	3492.0
26-27	1931.0
28-29	1620.0
30-31	3202.0
32-33	2445.0
34-35	2754.0
36-37	1572.0
38-39	1531.0
40-41	3136.0
42-43	2039.0
44-45	2192.0
46-47	3147.0
48-49	1548.0
50-51	18868.0
52-53	2116.0
54-55	25208.0
56-57	18432.0
58-59	47929.0
60-61	2770.0
62-63	12440.0
64-65	6513.0
66-67	8979.0
68-69	8869.0
70-71	28241.0
72-73	25068.0
74-75	18149.0
76-77	17591.0
78-79	6373.0
80-81	9890.0
82-83	15184.0
84-85	9456.0
86-87	3197.0
88-89	6426.0
90-91	13128.0
92-93	4265.0
94-95	4209.0
96-97	130872.0
98-99	0.0
100-101	5535231.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	97.302579125449
#Duplication Level	Relative count
1	100.0
2	7.217292807249386
3	2.153420174941791
4	1.2340318419231011
5	0.8495374740419105
6	0.7582908564596312
7	0.7897552073500723
8	0.7677301617267636
9	0.8155559750802341
10++	11.27178906299163
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA	13038	0.21494782594073059	No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC	13034	0.21488188091052943	No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC	12750	0.21019978376624598	No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG	12640	0.20838629543571363	No Hit
TAAAGAAACCCACAGAAACACACACACATACAATTAGCAAATGAATTCAA	12147	0.2002585704634188	No Hit
AAAAATAATAAACCGTTCATTTCTCAGGATGTGGTCATAGAATAAAGTTA	12087	0.19926939501040117	No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT	11689	0.19270786450538424	No Hit
GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG	11492	0.18946007176797636	No Hit
*	11339	0.18693767436278141	No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA	10650	0.175578642910629	No Hit
GATACAACATTCAAGACAGAGGAACTGGATGCCAAGCCAGACACAGACCC	10630	0.17524891775962312	No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG	10526	0.17353434697439254	No Hit
CATGTATTTTGAACCGGCACCCCTGTTACCACAGAGTGTGGGAGGAACTG	10517	0.1733859706564399	No Hit
CATGGGGTTGTGCCAATTAATTACCAAACATTGAGCCTGCAGGCTTTGAG	10337	0.17041844429738703	No Hit
AAGACAGGGGAAAGGAAGAAAAGGGAGTGAAGAGGGAATGGGAGAGGGAA	10137	0.16712119278732826	No Hit
CACTCAGTATGAGGAGCCGGCTGTCATCACATAGGGACAGGGCTCTCCCT	10009	0.16501095182089065	No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG	10005	0.1649450067906895	No Hit
CATGCCCTCCAGCGGCCCCGGGGACACCAGCAGCTCTGCTGCGGAGCGGG	9889	0.16303260091485539	No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA	9877	0.16283476582425188	No Hit
TAACTGTGGAAAAACTAAGTTGTCACCAGCGGTACCTCATAGCATAACTT	9067	0.1494808972085139	No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC	9064	0.14943143843586304	No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA	9051	0.1492171170877092	No Hit
AGCAACAAGAACAATACAAACCTCATTTCCTTTAGTCCTTCTGCCTCTAT	8991	0.14822794163469158	No Hit
CATGTAATTCAGGCAGTTATGGGAGCCCTAGAGGGCCTGAGAGTTGCTATTGGAC	8296	0.13676999263723738	No Hit
GTCCAATAGCAACTCTCAGGCCCTCTAGGGCTCCCATAACTGCCTGAATTACATG	8293	0.13672053386458652	No Hit
ACACTTACACCAAGGAATCTCCCAGGCATTATCAAAGCTACAGGAGACAA	8200	0.13518731191240915	No Hit
CATGTAGACTTACCTTTCCTCATAGAGCTATCCTGGTTAATAACAGGCCA	8187	0.13497299056425535	No Hit
CATGGGCTCGGCCACGCGCTACCACACCTACCTGCCGCCGCCCTACCCCG	7688	0.12674634804665874	No Hit
GAGAACTGGTAGGAGCCGGCCGAGGCGCCGTAGTACAGGTGGTAGGAGGG	7638	0.12592203516914408	No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT	7588	0.12509772229162935	No Hit
ACCTATGTTACACCATCTTCAGTGCCAGTCTTGGGCAAAATTGTGCAAGA	7552	0.12450421701981879	No Hit
TAAAACTGCATCAAGTCATGGGGCATGTGGAAGGTAGGGAGGCAAGATGA	7461	0.12300396758274205	No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT	7453	0.1228720775223397	No Hit
CATGGAGGGGAGCACTCAGCACCTCCCTCACCCCACACCCTTGGCTGCTC	7318	0.12064643275305004	No Hit
ATTGTTTCAAAAAAAATCAAACTGTAGTTGTTTTGGCGATAGGTCTCACG	7298	0.12031670760204417	No Hit
CATGGAATATATATTCTCCTGGCTGGTGCTTATGATTGCAAAACTTGGAT	7273	0.11990455116328681	No Hit
GCCTCAGCCTTCCTTCGCAGAAAGTTGAGCAGGTCGCCATAGCAACAGTA	7264	0.11975617484533418	No Hit
CACACTGTCATTAGTTGCAGCATCTCCTGTAGCAATTGGGCTAAATCTGT	7181	0.11838781546865979	No Hit
GACGCGACCGGACCGAGCGCCAAACGCGGCAGCCCAATCGCCATCGCTTT	6957	0.11469489377739397	No Hit
CACCAAAAACCCCAAGACAGAAATCTTAGGTATTCAGTTTCTTTTTCACA	6867	0.11321113059786754	No Hit
TAATCTACCTGAAGTCAATGAATGCAATTTTTCACACACACACACACACA	6828	0.11256816655340607	No Hit
TGAGTAGGGGGAGCAAATCGTGCCTTGTCATTTTATTTGGAGGTTTCCTG	6787	0.11189222999384403	No Hit
TGAGACCGCACCAGCGACACA	6776	0.1117108811607908	No Hit
TGTGTCGCTGGTGCGGTCTCA	6706	0.11055684313227022	No Hit
CGAGCCCGGCGGGCCCTGTGATTGGACGGGCGCCCGCCTCGCGTCCCGCC	6706	0.11055684313227022	No Hit
ATCCTTCACAAAGTCTTTGAGATATATTTTTATCAAATATTTAGCATGGATCCCGGTACACTTTCAATACTTA	6546	0.10791904192422323	No Hit
AATCTCTTTCCTTTATAAATTACCCAGTCTTGCATATGTCTTTATTAGCA	6538	0.10778715186382087	No Hit
TAAGTATTGAAAGTGTACCGGGATCCATGCTAAATATTTGATAAAAATATATCTCAAAGACTTTGTGAAGGAT	6505	0.1072431053646612	No Hit
CATGGCTCCACTCTCCACCGTCTCCACGCACACCTTCTCCAGCATCTGGG	6415	0.10575934218513473	No Hit
GAGGGAGAGGCCGGAGCTGCCCAGGACAGGCTCTGGGTCCTGCCCTTGCA	6415	0.10575934218513473	No Hit
GTTTTTATACACTAAGGACACTCCCAGTGATGAAAATGAATTCCCCTCCA	6171	0.10173669534286306	No Hit
CATGGTCACTCTCCCCAAAATATTATATTTTTTCTATAAAAAGAAAAAAA	6148	0.10135751141920629	No Hit
TAAACATTCCACTTCCTCCATAGGCTCCATGTTGGCTCAGGCAGACAAGC	6121	0.10091238246534835	No Hit
AGACTCTTACTCTTCTCAATCTTGCAGAGCTGAGCTTTGGCCTTTTTTAG	6101	0.10058265731434249	No Hit
AACTTTATAAACCAACTTCTGCTAGCTTCCAACTTCTCTTCTGCAGCTTC	6094	0.10046725351149043	No Hit
TAAATATAGCCCCAAAATGGTTGCTATAATAATCCCCATTTCATACTGGG	6087	0.10035184970863836	No Hit
GAAGCAGTAGTTTCACTTCTAGCTGGTCTCCCTCTGCAGCCTGAAGAAGG	6079	0.100219959648236	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	3431280	3.3771753	6.6232467	2
TTTTT	3146415	3.3559356	4.22344	65-69
GGGGG	856585	3.080178	7.846704	4
GGAGG	991670	2.7518895	5.529614	4
CCAGC	988200	2.6104617	7.9652824	5
CCTGG	953575	2.6021829	5.311066	9
CCCCA	994335	2.5838945	5.6402802	1
CCTCC	965395	2.5493364	9.5659275	6
CAGCC	957650	2.5297601	5.539111	6
TGGGG	861905	2.4305427	10.117945	3
GGCAG	819350	2.2366748	5.1211987	8
GCTGG	792260	2.1977642	5.7687	7
GAGGG	771640	2.141305	6.693945	9
GGGGA	755965	2.0978065	5.72526	7
CCACC	806075	2.094679	5.391248	1
CCCTC	761005	2.0096	5.9487286	5
CTCCA	955740	1.9799341	5.1599407	6
GCCAG	733300	1.9691764	7.3775535	4
AAGAA	1543480	1.9685211	5.0280623	2
TGGCT	896950	1.9512824	6.657417	3
TCCAG	924685	1.9473104	6.2876883	7
CCCAC	742045	1.9282898	6.0933833	9
CACAC	926600	1.888962	5.014705	8
CACCA	923615	1.8828769	5.0013533	8
TGCAG	875950	1.8752153	5.6388426	3
TCCTC	878495	1.8493977	5.5617895	5
TGTTT	1348625	1.8342156	5.758608	3
GGCTC	668815	1.8251097	7.0170255	4
GCCCA	683105	1.8045127	5.859571	4
AAACA	1397700	1.7535678	5.2002096	2
CCAGA	833645	1.7275972	5.2538104	8
AGGGG	620950	1.7231395	7.501309	6
GGCCC	475210	1.6266706	5.1926465	1
TGCCC	584255	1.5683933	11.632339	3
GCTCC	568205	1.525308	6.644112	2
GATGG	693135	1.5084122	5.972749	1
ATGGA	894575	1.5023719	10.900941	2
TAAAA	1494635	1.4949018	8.406264	1
CATGG	686815	1.4703192	42.917778	1
GGGGT	477120	1.345462	5.413382	4
TGCTC	607625	1.3003404	5.1721597	1
ATGTG	756550	1.291155	7.6515827	2
TGGGT	582645	1.2885056	6.220295	3
ATGAA	960925	1.2453992	9.473018	2
ATGGC	580990	1.2437712	11.03654	2
AGACA	752785	1.2238301	5.0523453	2
ATGGG	561240	1.2213804	14.515305	2
TGAGA	725535	1.2184818	5.282885	1
ATGCC	574910	1.2107133	12.036543	2
GAGAC	515350	1.0856607	5.120479	2
CATGT	646585	1.0855151	32.482143	1
CATGA	651490	1.0763115	30.554798	1
ATGTT	781240	1.0455954	7.9512677	2
TAAAG	785430	1.0179503	6.642147	1
ATGCT	598385	1.004595	7.6722145	2
ATGGT	585985	1.0000627	8.068158	2
AGACT	599775	0.99087435	5.0138197	1
ATGAC	594185	0.9816394	7.3691196	2
ATGAT	722625	0.9517264	6.805248	2
CATGC	439060	0.92462426	32.29644	1
ATGTA	693915	0.9139142	9.19232	2
GCGCC	253100	0.86637557	5.6018023	8
TAAGA	631075	0.81789964	5.0056934	1
ATGCA	455625	0.75272757	8.738035	2
TAACT	578555	0.7495719	5.581154	1
TAAGT	568225	0.7483753	6.5829115	1
TAAAC	546260	0.69644696	5.6095824	1
TAAGG	391775	0.6579568	5.0669847	1
TGCCG	198390	0.54138064	6.765231	5
>>END_MODULE