FastQCFastQC Report
Wed 20 Mar 2013
8016-8-19.h.sapiens.aligned.bam

Summary

[OK] Basic Statistics

Measure Value
Filename 8016-8-19.h.sapiens.aligned.bam
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 6065658
Filtered Sequences 0
Sequence length 1-100
%GC 43

[WARN] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA 13038 0.21494782594073059 No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC 13034 0.21488188091052943 No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC 12750 0.21019978376624598 No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG 12640 0.20838629543571363 No Hit
TAAAGAAACCCACAGAAACACACACACATACAATTAGCAAATGAATTCAA 12147 0.2002585704634188 No Hit
AAAAATAATAAACCGTTCATTTCTCAGGATGTGGTCATAGAATAAAGTTA 12087 0.19926939501040117 No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT 11689 0.19270786450538424 No Hit
GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG 11492 0.18946007176797636 No Hit
* 11339 0.18693767436278141 No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA 10650 0.175578642910629 No Hit
GATACAACATTCAAGACAGAGGAACTGGATGCCAAGCCAGACACAGACCC 10630 0.17524891775962312 No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG 10526 0.17353434697439254 No Hit
CATGTATTTTGAACCGGCACCCCTGTTACCACAGAGTGTGGGAGGAACTG 10517 0.1733859706564399 No Hit
CATGGGGTTGTGCCAATTAATTACCAAACATTGAGCCTGCAGGCTTTGAG 10337 0.17041844429738703 No Hit
AAGACAGGGGAAAGGAAGAAAAGGGAGTGAAGAGGGAATGGGAGAGGGAA 10137 0.16712119278732826 No Hit
CACTCAGTATGAGGAGCCGGCTGTCATCACATAGGGACAGGGCTCTCCCT 10009 0.16501095182089065 No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG 10005 0.1649450067906895 No Hit
CATGCCCTCCAGCGGCCCCGGGGACACCAGCAGCTCTGCTGCGGAGCGGG 9889 0.16303260091485539 No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA 9877 0.16283476582425188 No Hit
TAACTGTGGAAAAACTAAGTTGTCACCAGCGGTACCTCATAGCATAACTT 9067 0.1494808972085139 No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC 9064 0.14943143843586304 No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA 9051 0.1492171170877092 No Hit
AGCAACAAGAACAATACAAACCTCATTTCCTTTAGTCCTTCTGCCTCTAT 8991 0.14822794163469158 No Hit
CATGTAATTCAGGCAGTTATGGGAGCCCTAGAGGGCCTGAGAGTTGCTATTGGAC 8296 0.13676999263723738 No Hit
GTCCAATAGCAACTCTCAGGCCCTCTAGGGCTCCCATAACTGCCTGAATTACATG 8293 0.13672053386458652 No Hit
ACACTTACACCAAGGAATCTCCCAGGCATTATCAAAGCTACAGGAGACAA 8200 0.13518731191240915 No Hit
CATGTAGACTTACCTTTCCTCATAGAGCTATCCTGGTTAATAACAGGCCA 8187 0.13497299056425535 No Hit
CATGGGCTCGGCCACGCGCTACCACACCTACCTGCCGCCGCCCTACCCCG 7688 0.12674634804665874 No Hit
GAGAACTGGTAGGAGCCGGCCGAGGCGCCGTAGTACAGGTGGTAGGAGGG 7638 0.12592203516914408 No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT 7588 0.12509772229162935 No Hit
ACCTATGTTACACCATCTTCAGTGCCAGTCTTGGGCAAAATTGTGCAAGA 7552 0.12450421701981879 No Hit
TAAAACTGCATCAAGTCATGGGGCATGTGGAAGGTAGGGAGGCAAGATGA 7461 0.12300396758274205 No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT 7453 0.1228720775223397 No Hit
CATGGAGGGGAGCACTCAGCACCTCCCTCACCCCACACCCTTGGCTGCTC 7318 0.12064643275305004 No Hit
ATTGTTTCAAAAAAAATCAAACTGTAGTTGTTTTGGCGATAGGTCTCACG 7298 0.12031670760204417 No Hit
CATGGAATATATATTCTCCTGGCTGGTGCTTATGATTGCAAAACTTGGAT 7273 0.11990455116328681 No Hit
GCCTCAGCCTTCCTTCGCAGAAAGTTGAGCAGGTCGCCATAGCAACAGTA 7264 0.11975617484533418 No Hit
CACACTGTCATTAGTTGCAGCATCTCCTGTAGCAATTGGGCTAAATCTGT 7181 0.11838781546865979 No Hit
GACGCGACCGGACCGAGCGCCAAACGCGGCAGCCCAATCGCCATCGCTTT 6957 0.11469489377739397 No Hit
CACCAAAAACCCCAAGACAGAAATCTTAGGTATTCAGTTTCTTTTTCACA 6867 0.11321113059786754 No Hit
TAATCTACCTGAAGTCAATGAATGCAATTTTTCACACACACACACACACA 6828 0.11256816655340607 No Hit
TGAGTAGGGGGAGCAAATCGTGCCTTGTCATTTTATTTGGAGGTTTCCTG 6787 0.11189222999384403 No Hit
TGAGACCGCACCAGCGACACA 6776 0.1117108811607908 No Hit
TGTGTCGCTGGTGCGGTCTCA 6706 0.11055684313227022 No Hit
CGAGCCCGGCGGGCCCTGTGATTGGACGGGCGCCCGCCTCGCGTCCCGCC 6706 0.11055684313227022 No Hit
ATCCTTCACAAAGTCTTTGAGATATATTTTTATCAAATATTTAGCATGGATCCCGGTACACTTTCAATACTTA 6546 0.10791904192422323 No Hit
AATCTCTTTCCTTTATAAATTACCCAGTCTTGCATATGTCTTTATTAGCA 6538 0.10778715186382087 No Hit
TAAGTATTGAAAGTGTACCGGGATCCATGCTAAATATTTGATAAAAATATATCTCAAAGACTTTGTGAAGGAT 6505 0.1072431053646612 No Hit
CATGGCTCCACTCTCCACCGTCTCCACGCACACCTTCTCCAGCATCTGGG 6415 0.10575934218513473 No Hit
GAGGGAGAGGCCGGAGCTGCCCAGGACAGGCTCTGGGTCCTGCCCTTGCA 6415 0.10575934218513473 No Hit
GTTTTTATACACTAAGGACACTCCCAGTGATGAAAATGAATTCCCCTCCA 6171 0.10173669534286306 No Hit
CATGGTCACTCTCCCCAAAATATTATATTTTTTCTATAAAAAGAAAAAAA 6148 0.10135751141920629 No Hit
TAAACATTCCACTTCCTCCATAGGCTCCATGTTGGCTCAGGCAGACAAGC 6121 0.10091238246534835 No Hit
AGACTCTTACTCTTCTCAATCTTGCAGAGCTGAGCTTTGGCCTTTTTTAG 6101 0.10058265731434249 No Hit
AACTTTATAAACCAACTTCTGCTAGCTTCCAACTTCTCTTCTGCAGCTTC 6094 0.10046725351149043 No Hit
TAAATATAGCCCCAAAATGGTTGCTATAATAATCCCCATTTCATACTGGG 6087 0.10035184970863836 No Hit
GAAGCAGTAGTTTCACTTCTAGCTGGTCTCCCTCTGCAGCCTGAAGAAGG 6079 0.100219959648236 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 3431280 3.3771753 6.6232467 2
TTTTT 3146415 3.3559356 4.22344 65-69
GGGGG 856585 3.080178 7.846704 4
GGAGG 991670 2.7518895 5.529614 4
CCAGC 988200 2.6104617 7.9652824 5
CCTGG 953575 2.6021829 5.311066 9
CCCCA 994335 2.5838945 5.6402802 1
CCTCC 965395 2.5493364 9.5659275 6
CAGCC 957650 2.5297601 5.539111 6
TGGGG 861905 2.4305427 10.117945 3
GGCAG 819350 2.2366748 5.1211987 8
GCTGG 792260 2.1977642 5.7687 7
GAGGG 771640 2.141305 6.693945 9
GGGGA 755965 2.0978065 5.72526 7
CCACC 806075 2.094679 5.391248 1
CCCTC 761005 2.0096 5.9487286 5
CTCCA 955740 1.9799341 5.1599407 6
GCCAG 733300 1.9691764 7.3775535 4
AAGAA 1543480 1.9685211 5.0280623 2
TGGCT 896950 1.9512824 6.657417 3
TCCAG 924685 1.9473104 6.2876883 7
CCCAC 742045 1.9282898 6.0933833 9
CACAC 926600 1.888962 5.014705 8
CACCA 923615 1.8828769 5.0013533 8
TGCAG 875950 1.8752153 5.6388426 3
TCCTC 878495 1.8493977 5.5617895 5
TGTTT 1348625 1.8342156 5.758608 3
GGCTC 668815 1.8251097 7.0170255 4
GCCCA 683105 1.8045127 5.859571 4
AAACA 1397700 1.7535678 5.2002096 2
CCAGA 833645 1.7275972 5.2538104 8
AGGGG 620950 1.7231395 7.501309 6
GGCCC 475210 1.6266706 5.1926465 1
TGCCC 584255 1.5683933 11.632339 3
GCTCC 568205 1.525308 6.644112 2
GATGG 693135 1.5084122 5.972749 1
ATGGA 894575 1.5023719 10.900941 2
TAAAA 1494635 1.4949018 8.406264 1
CATGG 686815 1.4703192 42.917778 1
GGGGT 477120 1.345462 5.413382 4
TGCTC 607625 1.3003404 5.1721597 1
ATGTG 756550 1.291155 7.6515827 2
TGGGT 582645 1.2885056 6.220295 3
ATGAA 960925 1.2453992 9.473018 2
ATGGC 580990 1.2437712 11.03654 2
AGACA 752785 1.2238301 5.0523453 2
ATGGG 561240 1.2213804 14.515305 2
TGAGA 725535 1.2184818 5.282885 1
ATGCC 574910 1.2107133 12.036543 2
GAGAC 515350 1.0856607 5.120479 2
CATGT 646585 1.0855151 32.482143 1
CATGA 651490 1.0763115 30.554798 1
ATGTT 781240 1.0455954 7.9512677 2
TAAAG 785430 1.0179503 6.642147 1
ATGCT 598385 1.004595 7.6722145 2
ATGGT 585985 1.0000627 8.068158 2
AGACT 599775 0.99087435 5.0138197 1
ATGAC 594185 0.9816394 7.3691196 2
ATGAT 722625 0.9517264 6.805248 2
CATGC 439060 0.92462426 32.29644 1
ATGTA 693915 0.9139142 9.19232 2
GCGCC 253100 0.86637557 5.6018023 8
TAAGA 631075 0.81789964 5.0056934 1
ATGCA 455625 0.75272757 8.738035 2
TAACT 578555 0.7495719 5.581154 1
TAAGT 568225 0.7483753 6.5829115 1
TAAAC 546260 0.69644696 5.6095824 1
TAAGG 391775 0.6579568 5.0669847 1
TGCCG 198390 0.54138064 6.765231 5
Produced by FastQC (version 0.10.0)