FastQCFastQC Report
Wed 20 Mar 2013
8016-8-20.h.sapiens.aligned.bam

Summary

[OK] Basic Statistics

Measure Value
Filename 8016-8-20.h.sapiens.aligned.bam
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 4696432
Filtered Sequences 0
Sequence length 1-100
%GC 43

[WARN] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA 10770 0.22932302650182096 No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC 10622 0.2261716980039315 No Hit
TAAAGAAACCCACAGAAACACACACACATACAATTAGCAAATGAATTCAA 10606 0.2258310138419975 No Hit
AAAAATAATAAACCGTTCATTTCTCAGGATGTGGTCATAGAATAAAGTTA 10559 0.2248302541163164 No Hit
* 10172 0.21658995594953787 No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC 9262 0.19721354423954182 No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG 9213 0.196170198993619 No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT 8646 0.184097204005083 No Hit
GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG 8631 0.1837778126032699 No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG 8612 0.18337325016097328 No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA 8611 0.1833519574008524 No Hit
CATGGGGTTGTGCCAATTAATTACCAAACATTGAGCCTGCAGGCTTTGAG 8473 0.18041355650417168 No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG 8458 0.18009416510235857 No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA 8399 0.17883789225522695 No Hit
AAGACAGGGGAAAGGAAGAAAAGGGAGTGAAGAGGGAATGGGAGAGGGAA 8218 0.1749839026733486 No Hit
ACACTTACACCAAGGAATCTCCCAGGCATTATCAAAGCTACAGGAGACAA 7642 0.16271927284372478 No Hit
CATGTAGACTTACCTTTCCTCATAGAGCTATCCTGGTTAATAACAGGCCA 7635 0.16257022352287864 No Hit
CATGTAATTCAGGCAGTTATGGGAGCCCTAGAGGGCCTGAGAGTTGCTATTGGAC 7480 0.15926984570414304 No Hit
GTCCAATAGCAACTCTCAGGCCCTCTAGGGCTCCCATAACTGCCTGAATTACATG 7399 0.15754513213435223 No Hit
ACAGATTTTGGGGTTGTGTTGTCACCCAAGAGATTGTTGTTTGCCATACT 7398 0.15752383937423134 No Hit
GATACAACATTCAAGACAGAGGAACTGGATGCCAAGCCAGACACAGACCC 7394 0.15743866833374784 No Hit
ATTGTTTCAAAAAAAATCAAACTGTAGTTGTTTTGGCGATAGGTCTCACG 7377 0.15707669141169298 No Hit
CATGTATTTTGAACCGGCACCCCTGTTACCACAGAGTGTGGGAGGAACTG 7338 0.15624627376697883 No Hit
TGAGACCGCACCAGCGACACA 6998 0.14900673532588143 No Hit
ATCCTTCACAAAGTCTTTGAGATATATTTTTATCAAATATTTAGCATGGATCCCGGTACACTTTCAATACTTA 6964 0.14828278148177168 No Hit
TAAGTATTGAAAGTGTACCGGGATCCATGCTAAATATTTGATAAAAATATATCTCAAAGACTTTGTGAAGGAT 6907 0.14706909415488184 No Hit
TGTGTCGCTGGTGCGGTCTCA 6887 0.14664323895246434 No Hit
TAATCTACCTGAAGTCAATGAATGCAATTTTTCACACACACACACACACA 6883 0.14655806791198084 No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC 6805 0.1448972326225526 No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA 6791 0.14459913398086036 No Hit
AGCAACAAGAACAATACAAACCTCATTTCCTTTAGTCCTTCTGCCTCTAT 6745 0.14361966701530013 No Hit
TAACTGTGGAAAAACTAAGTTGTCACCAGCGGTACCTCATAGCATAACTT 6734 0.14338544665397052 No Hit
CACTCAGTATGAGGAGCCGGCTGTCATCACATAGGGACAGGGCTCTCCCT 6463 0.13761510866121346 No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT 6393 0.13612461545275223 No Hit
CATGCCCTCCAGCGGCCCCGGGGACACCAGCAGCTCTGCTGCGGAGCGGG 6361 0.13544324712888423 No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT 6335 0.13488963536574147 No Hit
AACTTTATAAACCAACTTCTGCTAGCTTCCAACTTCTCTTCTGCAGCTTC 5844 0.12443489014639199 No Hit
TAATCCAGAGCAAGGCCTTGACTCCCTTCAGTTCTATGAAGAGAATGAGG 5789 0.12326378833974387 No Hit
GTTTTTATACACTAAGGACACTCCCAGTGATGAAAATGAATTCCCCTCCA 5616 0.11958014083883253 No Hit
CACACTGTCATTAGTTGCAGCATCTCCTGTAGCAATTGGGCTAAATCTGT 5571 0.1186219666333932 No Hit
CATGGAATATATATTCTCCTGGCTGGTGCTTATGATTGCAAAACTTGGAT 5522 0.11757862138747031 No Hit
TGAGATTTTTTTGTGTATGTTTTTGACTCTTTTGAGTGGTAATCATATGT 5346 0.11383109560619635 No Hit
CATGACTATAAATATAAAGCAAATATGATTATGCTCACAAGCTCTGGAAT 5318 0.11323489832281187 No Hit
CATGGGCTCGGCCACGCGCTACCACACCTACCTGCCGCCGCCCTACCCCG 5268 0.11217026031676815 No Hit
GCACGTGGGAGAGTAAGCAGGCCAGGTAGAGCTGCAGAAAACTAAGGCCC 5267 0.11214896755664726 No Hit
CATGTAGGTTATATATGCTAGAATTGCATTTAATCACTGTGAAAAGACTG 5236 0.11148889199290013 No Hit
ACCTATGTTACACCATCTTCAGTGCCAGTCTTGGGCAAAATTGTGCAAGA 5234 0.1114463064726584 No Hit
ATAATCCATACCACCAGTATAGCAGCAATTCTAACAGCCCCCCTACTGTC 5220 0.11114820783096616 No Hit
GAGAACTGGTAGGAGCCGGCCGAGGCGCCGTAGTACAGGTGGTAGGAGGG 5219 0.11112691507084527 No Hit
TAACCAGAGTTAGTAGTGTATGTAAAATTCTGACTGTTCTTGCAACAATA 5201 0.11074364538866951 No Hit
ATTCTTACTTGTTTGTTTGTTTGTTTGTTTGTTTTTTGTAGGGTACAGGC 5192 0.11055201054758165 No Hit
AAGTCCTCCTTATTCAAGATTTTGAAATTCTTAGCCTGGGAGTGCTGGAG 5168 0.11004098430468065 No Hit
TAAAACTGCATCAAGTCATGGGGCATGTGGAAGGTAGGGAGGCAAGATGA 5098 0.10855049109621943 No Hit
TAACACTGATATACAAATTGGGACTCTTCATTCTGGAGAAAGCATCAGGT 5074 0.10803946485331843 No Hit
AAGATTGATAGACCTGAAGATGCTGGGGAGAAAGAACATGTCACTAAGAG 5040 0.10731551100920868 No Hit
AGACTGCTGAAGTTCTTCTTTGTCCCACTGAGGTTGTACTCTTCATTCTC 5025 0.10699611960739558 No Hit
TAAACTTATACAGCGAGAAAATGTCATTGAATATAAACACTGTTTGATTA 5015 0.10678319200618683 No Hit
GCCTCAGCCTTCCTTCGCAGAAAGTTGAGCAGGTCGCCATAGCAACAGTA 5009 0.10665543544546158 No Hit
TAACTCTGCTTACAGAATGGCAGTTATCCATCTTTGGCTCATTTTTGTGG 4988 0.1062082874829232 No Hit
AATCTCTTTCCTTTATAAATTACCCAGTCTTGCATATGTCTTTATTAGCA 4978 0.10599535988171445 No Hit
CATGGAGGGGAGCACTCAGCACCTCCCTCACCCCACACCCTTGGCTGCTC 4962 0.10565467571978046 No Hit
GTATCTTAATGATGTATATAATTGCCTTCAATCCCCTTCTCACCCCACCC 4894 0.10420676803156098 No Hit
CATGCTTTACTCAACTGAAATAATCAAGCCCCTATTGCTGTGGGGGAAGC 4891 0.10414288975119837 No Hit
ATCCAAGTAAGCCATTCTCAAGAGGCAGTCAGCCTGCAGATGTGGATCTA 4863 0.10354669246781387 No Hit
CATGACTATAAATATGAAGCAAATATGATTATCCTCACAAGTTCTGGAAT 4858 0.10344022866720949 No Hit
AGCAATTCCATTTATGTGTTTGTTAGAGGTAAATGCTTGGCTTTCTGCAGTGCTGTGCTTTCAAGAATTTA 4856 0.10339764314696775 No Hit
CATGATATACATACTCTCTGTAAAGTTACTCTTGGTTGCTTGATAGGTAG 4851 0.10329117934636337 No Hit
TAAATTCTTGAAAGCACAGCACTGCAGAAAGCCAAGCATTTACCTCTAACAAACACATAAATGGAATTGCT 4837 0.10299308070467113 No Hit
TAACTAACTCATAAATGCCTATTTTTTCTTACAGAAAAGGGATTTATAAA 4828 0.10280144586358325 No Hit
AACCTGACAGAAAAACAAAACTCTTCATATTGGTCAATTTCATAATCTTC 4827 0.10278015310346236 No Hit
AAACCTGGAGTTTCTTATGTTGTCCCAACCAAGGCCGACAAAAGGAGATC 4792 0.10203490649923176 No Hit
TAGACATCTATAACTAACAACCACTTTTCTTACTATCATTGAAGTCAATA 4779 0.10175810061766037 No Hit
TGAGTAGGGGGAGCAAATCGTGCCTTGTCATTTTATTTGGAGGTTTCCTG 4761 0.10137483093548465 No Hit
TAAAGTGTTGGTTGTTGTGAGGGCTTATACGAAAGCAAGAAACAAGGCAG 4743 0.10099156125330891 No Hit
CAACACCCAACACTTATAAGCGGAAGAACACAGAAACAGCTCTAGACAAC 4735 0.1008212191723419 No Hit
CATGCCCACAGAGACTTGCACAACATGCAGAATGGCAGCACATTGGTAAG 4701 0.10009726532823215 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 2771005 3.3748276 6.827033 2
TTTTT 2479580 3.2620363 3.9985847 65-69
GGGGG 628865 3.1260638 7.4705596 4
GGAGG 737355 2.766635 5.472763 4
CCAGC 732545 2.6113365 8.281346 5
CCTGG 706520 2.6017637 5.1842256 9
CCTCC 719720 2.5613906 9.202481 6
CCCCA 726260 2.5451014 5.6099057 1
CAGCC 710975 2.5344448 5.959687 6
TGGGG 628925 2.3964775 10.195924 3
CTCCC 655025 2.3311496 5.3217564 3
GGCAG 605015 2.2316484 5.319729 8
GCTGG 590025 2.2101889 7.063606 7
GAGGG 573550 2.152021 7.0948024 9
GGGGA 560060 2.101405 5.8360453 7
CCACC 589220 2.0648592 5.6751747 1
CACAC 751345 2.021635 5.1928663 8
CTCCT 716640 1.9886708 5.2937584 4
TCCAG 702845 1.9536093 6.3950534 7
GCCAG 536370 1.9449507 7.3026037 4
AAGAA 1202195 1.9397888 5.325443 2
CCCAC 549410 1.9253492 6.8484297 9
TGGCT 669815 1.9233035 6.3001595 3
CACCA 703150 1.8919572 5.7556458 8
TGCAG 662520 1.8732369 5.8494115 3
TGTTT 1073140 1.8417598 6.03137 3
GGCTC 492945 1.8152726 6.8328524 4
TCCTC 651860 1.8089068 6.216554 5
GCCCA 498235 1.7760812 5.822223 4
AGGGG 466685 1.7510521 7.66419 6
CCAGA 627105 1.7164024 5.139496 8
GGCCC 342365 1.6168998 5.926377 1
GAAAG 755445 1.6149073 5.3076835 5
GCTCC 428215 1.550207 7.560984 2
TGCCC 422995 1.5313098 11.186018 3
AGCAA 726020 1.5257304 5.0611434 8
GATGG 517965 1.4897373 5.935342 1
ATGGA 684380 1.4857349 10.732945 2
TAAAA 1189285 1.4709553 8.453122 1
CATGG 517830 1.4641343 41.495678 1
GCAGT 482890 1.3653436 5.316101 4
GGGGT 350235 1.3345474 5.744223 4
TGCTC 469510 1.3253245 5.968506 1
ATGTG 597220 1.316672 7.511504 2
TGGGT 431000 1.2588842 5.9229064 3
ATGGC 439170 1.2417277 10.362631 2
ATGAA 755910 1.2386508 9.086189 2
AGACA 582015 1.223104 5.231553 2
ATGCC 438810 1.2197046 11.341533 2
ATGGG 423705 1.2186328 14.542208 2
TGAGA 559800 1.2152816 5.415049 1
AACAC 538070 1.11161 5.3411226 2
CATGT 507785 1.1005445 32.75262 1
GAGAC 388485 1.081605 5.59976 2
CATGA 500635 1.0684389 30.141596 1
ATGTT 616765 1.0423104 7.9632807 2
ATGCT 471530 1.0219674 7.564957 2
TAAAG 620700 1.0170926 7.0774403 1
ATGGT 452965 0.99863774 7.1809907 2
ATGAC 455725 0.9725934 7.690608 2
AGACC 347650 0.95152706 5.188051 3
ATGAT 569995 0.9485257 6.4049726 2
CATGC 340260 0.9457777 31.972906 1
ATGTA 567240 0.9439411 9.340552 2
GCGCC 183255 0.86546516 5.5483994 8
TAAGA 492915 0.8077014 5.1257277 1
ATGCA 356810 0.7614923 8.655412 2
TAACT 459855 0.7522867 6.137232 1
TAAGT 449715 0.7483684 7.0789833 1
TAAAC 434490 0.69991124 5.5504937 1
TAAGG 305040 0.66221774 5.1603184 1
TGCCG 148320 0.5461892 7.987105 5
CCGTC 122590 0.4437954 5.446999 7
GCCGT 114945 0.42328557 5.482411 6
Produced by FastQC (version 0.10.0)