##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	8016-8-21.h.sapiens.aligned.bam	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	5023518	
Filtered Sequences	0	
Sequence length	1-100	
%GC	43	
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.95605330766208	24.0	24.0	31.0	23.0	31.0
2	31.012384050887647	31.0	31.0	32.0	29.0	33.0
3	32.66306686337113	33.0	33.0	34.0	29.0	35.0
4	33.873152261819115	34.0	33.0	36.0	29.0	37.0
5	35.41154340644897	36.0	35.0	37.0	33.0	37.0
6	35.72641536919751	36.0	36.0	38.0	33.0	38.0
7	35.75159386120339	37.0	35.0	37.0	34.0	37.0
8	35.598726187143505	36.0	36.0	37.0	32.0	38.0
9	35.622783313814935	37.0	36.0	37.0	31.0	38.0
10-14	35.98979989428143	37.0	36.0	37.4	32.4	38.0
15-19	36.97800608752629	38.0	37.2	38.4	32.8	39.4
20-24	36.613465016759825	37.4	36.0	38.8	32.8	39.8
25-29	36.27090046017113	37.6	36.2	38.0	32.8	38.2
30-34	36.108603667910785	37.6	35.6	38.2	32.4	38.6
35-39	36.14979101955002	37.8	36.4	38.0	31.6	38.6
40-44	36.06986541336956	37.4	36.0	38.4	32.4	38.8
45-49	36.02392309797739	37.6	35.8	38.2	32.0	38.6
50-54	36.23848778250168	37.6	36.2	38.4	33.0	39.0
55-59	35.958711686039386	37.6	35.8	38.2	32.4	38.6
60-64	35.96506680434139	37.6	35.6	38.2	32.6	38.6
65-69	35.7686138720263	37.4	36.0	38.2	31.4	38.6
70-74	35.80937396985898	37.4	36.0	38.4	31.6	39.0
75-79	35.52012658866388	37.4	35.4	37.6	31.6	38.4
80-84	35.63508436668046	37.2	35.8	38.2	31.0	38.6
85-89	35.35248239625416	37.2	35.4	37.6	31.0	38.2
90-94	35.36812443581535	37.0	35.0	38.2	30.8	38.4
95-99	35.19802187266964	37.4	35.2	37.6	30.8	38.2
100	34.13165671045909	36.0	33.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
1	4.0
2	19.0
3	88.0
4	0.0
5	9.0
6	35.0
7	148.0
8	197.0
9	6442.0
10	483.0
11	696.0
12	1077.0
13	1784.0
14	2418.0
15	3261.0
16	3923.0
17	4795.0
18	5953.0
19	7500.0
20	8650.0
21	9340.0
22	9833.0
23	11201.0
24	13804.0
25	17778.0
26	24157.0
27	33013.0
28	45287.0
29	60084.0
30	78196.0
31	101794.0
32	131245.0
33	177766.0
34	263334.0
35	471805.0
36	1422745.0
37	2104652.0
38	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.714080946839204	20.43869402809473	22.141272103802624	38.705952921263446
2	16.913205111414246	53.164592793442544	15.62715851958523	14.295043575557978
3	17.97241131853565	36.3958497373693	30.644606352971664	14.98713259112338
4	33.307077917780084	25.553716148057422	21.470311245292166	19.668894688870335
5	19.941198411479473	28.253744412552585	27.29197541822331	24.513081757744636
6	19.60894578278992	30.275561097008037	27.051013136581613	23.06447998362043
7	21.46020062468839	28.783666652889416	28.311266774049336	21.444865948372858
8	20.56354294771611	28.140686459797838	29.938433749638836	21.357336842847218
9	20.84617922049293	29.309309261338857	27.904980902891484	21.939530615276727
10-14	21.24742216700552	28.349475619477644	27.66277233955195	22.74032987396489
15-19	20.67868719975216	28.251970592564966	28.170040920892863	22.899301286790006
20-24	20.9096944568648	27.63471413595972	28.457384709935695	22.998206697239787
25-29	21.114305081997813	27.722282265550817	29.087696777500156	22.075715874951214
30-34	21.811823642503366	28.107802146698614	28.293034334346252	21.78733987645177
35-39	21.68875168595442	27.00752950203708	29.55403872138087	21.749680090627628
40-44	21.751243949586048	28.183443531452074	28.09251924813294	21.972793270828937
45-49	21.529751307151653	28.882653138021503	28.541835491241123	21.04576006358572
50-54	21.82319938714463	28.123760380415995	28.398038422326565	21.655001810112807
55-59	21.340586831712134	28.927872710555736	28.108629918452426	21.622910539279708
60-64	21.67565668169687	28.42553152167851	28.939900419818223	20.958911376806405
65-69	21.55506766300101	29.381999911718886	28.54958603528611	20.513346389993988
70-74	21.485692746620053	28.927965164912028	28.148335390317232	21.43800669815069
75-79	22.02243486223314	28.905075793180128	28.22115536978212	20.85133397480461
80-84	21.262909912514957	28.806570322605364	28.45677678415492	21.47374298072476
85-89	21.349246001746376	28.615053936350233	28.653637088067363	21.382062973836025
90-94	21.403806846500228	28.260606656861487	28.69318479153003	21.642401705108256
95-99	21.459075670180216	27.781797729363195	28.890602466817167	21.86852413363942
100	22.52406315918119	28.097241292155566	27.462916113002834	21.91577943566041
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	57.42003386810265
2	31.208248686972222
3	32.959543909659025
4	52.975972606650416
5	44.454280169224106
6	42.67342576641035
7	42.90506657306125
8	41.92087979056333
9	42.78570983576966
10-14	43.987752040970406
15-19	43.57798848654216
20-24	43.907901154104586
25-29	43.19002095694903
30-34	43.59916351895514
35-39	43.43843177658205
40-44	43.724037220414985
45-49	42.575511370737374
50-54	43.47820119725744
55-59	42.96349737099184
60-64	42.63456805850328
65-69	42.068414052995
70-74	42.92369944477074
75-79	42.87376883703775
80-84	42.73665289323972
85-89	42.7313089755824
90-94	43.04620855160849
95-99	43.32759980381964
100	44.4398425948416
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6588.0
1	6587.5
2	6589.5
3	6596.0
4	6599.5
5	6595.5
6	6597.0
7	6611.5
8	6633.5
9	6661.5
10	6702.5
11	6743.0
12	6784.0
13	6860.5
14	6900.5
15	7272.5
16	7498.5
17	7422.5
18	7771.5
19	8613.5
20	10180.5
21	12061.5
22	16736.0
23	23596.0
24	38521.5
25	52208.0
26	63709.5
27	80179.5
28	85309.0
29	97318.5
30	123484.5
31	140689.5
32	147066.0
33	163921.5
34	185718.0
35	179518.0
36	168602.0
37	161076.5
38	177935.0
39	197411.0
40	200829.5
41	212389.0
42	205627.5
43	204083.0
44	206293.5
45	188929.5
46	170674.5
47	147589.5
48	134608.0
49	152064.0
50	163348.0
51	161626.5
52	131369.5
53	83224.0
54	73826.5
55	73768.5
56	56670.5
57	48272.0
58	62684.5
59	60544.5
60	57872.0
61	74233.5
62	67283.0
63	58933.5
64	53385.5
65	46334.0
66	47723.5
67	45319.0
68	33665.5
69	26731.0
70	25061.0
71	22625.5
72	25425.5
73	18211.5
74	9059.0
75	10960.0
76	11714.0
77	11181.0
78	9919.5
79	9905.5
80	8729.0
81	7155.0
82	6325.0
83	6325.0
84	7081.5
85	6969.0
86	7068.5
87	5898.5
88	5079.5
89	5417.0
90	5828.5
91	6292.0
92	5356.5
93	4412.0
94	4396.5
95	4396.5
96	4385.0
97	4377.5
98	4378.5
99	4377.5
100	4377.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05336897369532666
2	0.007913322606827861
3	0.0022524933406928072
4	0.007236162633054192
5	0.004627319294206157
6	8.97994598462713E-4
7	4.1930579732195377E-4
8	2.5957875559541973E-4
9	1.3977370636938797E-4
10-14	0.0013498259702922474
15-19	0.01808525335710511
20-24	0.016613849672323485
25-29	0.008463610275393817
30-34	0.00797278868306716
35-39	0.005494332418873402
40-44	0.013125692086985444
45-49	0.011854735508866942
50-54	0.010318723885157826
55-59	0.011095814747639588
60-64	0.05462967307377216
65-69	0.04394732215199996
70-74	0.02769311014015207
75-79	0.07391035290287953
80-84	0.0644864956782062
85-89	0.014809307807502843
90-94	0.006774794262612127
95-99	0.009048936458134725
100	0.008015482583517058
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
0-1	6662.0
2-3	385.0
4-5	5305.0
6-7	3052.0
8-9	31.0
10-11	22.0
12-13	56.0
14-15	155.0
16-17	183.0
18-19	2764.0
20-21	23051.0
22-23	2859.0
24-25	3883.0
26-27	1913.0
28-29	1695.0
30-31	2484.0
32-33	1504.0
34-35	1829.0
36-37	1103.0
38-39	1349.0
40-41	2282.0
42-43	1532.0
44-45	1566.0
46-47	2566.0
48-49	1512.0
50-51	16450.0
52-53	3193.0
54-55	22610.0
56-57	16802.0
58-59	40330.0
60-61	1774.0
62-63	10447.0
64-65	6187.0
66-67	8081.0
68-69	7344.0
70-71	25102.0
72-73	22930.0
74-75	15493.0
76-77	17468.0
78-79	6382.0
80-81	11490.0
82-83	15356.0
84-85	6921.0
86-87	3338.0
88-89	5365.0
90-91	11529.0
92-93	3773.0
94-95	3401.0
96-97	105846.0
98-99	0.0
100-101	4566163.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	97.24093148471775
#Duplication Level	Relative count
1	100.0
2	6.579991630712399
3	2.0663482489430165
4	1.2705444365882166
5	1.0302881632299679
6	0.9538101903291438
7	0.9429878356733669
8	0.9574176418810696
9	0.9393803841214412
10++	12.014978138843595
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TAAAGAAACCCACAGAAACACACACACATACAATTAGCAAATGAATTCAA	11875	0.2363881248161149	No Hit
AAAAATAATAAACCGTTCATTTCTCAGGATGTGGTCATAGAATAAAGTTA	11826	0.2354127127642421	No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA	9858	0.19623697974208515	No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC	9820	0.19548053774267357	No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC	8944	0.1780425590193964	No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG	8907	0.17730602338839038	No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT	8769	0.17455894454842202	No Hit
CATGGGGTTGTGCCAATTAATTACCAAACATTGAGCCTGCAGGCTTTGAG	8666	0.1725085886026486	No Hit
GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG	8662	0.1724289631290263	No Hit
AAGACAGGGGAAAGGAAGAAAAGGGAGTGAAGAGGGAATGGGAGAGGGAA	8429	0.16779077929052907	No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG	7898	0.15722049766717267	No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA	7825	0.15576733277356625	No Hit
GATACAACATTCAAGACAGAGGAACTGGATGCCAAGCCAGACACAGACCC	7696	0.15319941124924805	No Hit
CATGTATTTTGAACCGGCACCCCTGTTACCACAGAGTGTGGGAGGAACTG	7672	0.15272165840751442	No Hit
CATGTAATTCAGGCAGTTATGGGAGCCCTAGAGGGCCTGAGAGTTGCTATTGGAC	7660	0.1524827819866476	No Hit
GTCCAATAGCAACTCTCAGGCCCTCTAGGGCTCCCATAACTGCCTGAATTACATG	7621	0.15170643361883046	No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA	7472	0.1487403847264009	No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA	7412	0.14754600262206685	No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG	7372	0.14674974788584416	No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC	7362	0.1465506842017885	No Hit
AGCAACAAGAACAATACAAACCTCATTTCCTTTAGTCCTTCTGCCTCTAT	7016	0.1396630807334621	No Hit
TAACTGTGGAAAAACTAAGTTGTCACCAGCGGTACCTCATAGCATAACTT	7000	0.139344578838973	No Hit
ACACTTACACCAAGGAATCTCCCAGGCATTATCAAAGCTACAGGAGACAA	6813	0.13562208794713188	No Hit
CATGTAGACTTACCTTTCCTCATAGAGCTATCCTGGTTAATAACAGGCCA	6800	0.1353633051578595	No Hit
TGAGACCGCACCAGCGACACA	6682	0.1330143536860025	No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT	6628	0.13193940979210186	No Hit
CTTGGGGGAGTGTGCCTCCAGCAGAGAAGTAATTCTGGCCTGGCAACCTA	6623	0.13183987795007404	No Hit
ACCTATGTTACACCATCTTCAGTGCCAGTCTTGGGCAAAATTGTGCAAGA	6617	0.13172043973964065	No Hit
AGGACAACAGCTGATCCATACATTTGCTGACAGGTGTACCTGCGTTTCCC	6616	0.13170053337123505	No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT	6565	0.13068530858255112	No Hit
TGTGTCGCTGGTGCGGTCTCA	6564	0.13066540221414555	No Hit
TAAAACTGCATCAAGTCATGGGGCATGTGGAAGGTAGGGAGGCAAGATGA	6492	0.12923214368894467	No Hit
CATGGAATATATATTCTCCTGGCTGGTGCTTATGATTGCAAAACTTGGAT	6414	0.1276794469533104	No Hit
CACACTGTCATTAGTTGCAGCATCTCCTGTAGCAATTGGGCTAAATCTGT	6346	0.1263258139017318	No Hit
ATTGTTTCAAAAAAAATCAAACTGTAGTTGTTTTGGCGATAGGTCTCACG	6294	0.1252906827446423	No Hit
AACTTTATAAACCAACTTCTGCTAGCTTCCAACTTCTCTTCTGCAGCTTC	6176	0.12294173127278532	No Hit
TAATCCAGAGCAAGGCCTTGACTCCCTTCAGTTCTATGAAGAGAATGAGG	6169	0.12280238669394636	No Hit
ATCCTTCACAAAGTCTTTGAGATATATTTTTATCAAATATTTAGCATGGATCCCGGTACACTTTCAATACTTA	6134	0.12210566379975149	No Hit
*	6089	0.12120987722150094	No Hit
TAAGTATTGAAAGTGTACCGGGATCCATGCTAAATATTTGATAAAAATATATCTCAAAGACTTTGTGAAGGAT	6084	0.1211103453794731	No Hit
CACCAAAAACCCCAAGACAGAAATCTTAGGTATTCAGTTTCTTTTTCACA	5972	0.11888083211804953	No Hit
TAATCTACCTGAAGTCAATGAATGCAATTTTTCACACACACACACACACA	5948	0.11840307927631591	No Hit
TTAGCTTTGAAGTGGATCCTACCAGTTTTATCATCTTTTCACATAAAAGT	5902	0.11748738632965981	No Hit
CACTCAGTATGAGGAGCCGGCTGTCATCACATAGGGACAGGGCTCTCCCT	5761	0.11468058838447479	No Hit
CATGGGTATTATTTCTTTGCTTTTTTTGTGTGGTGGGGGGCTTTATTTGC	5737	0.11420283554274117	No Hit
GAAGCAGTAGTTTCACTTCTAGCTGGTCTCCCTCTGCAGCCTGAAGAAGG	5703	0.11352601901695188	No Hit
CATGCCCTCCAGCGGCCCCGGGGACACCAGCAGCTCTGCTGCGGAGCGGG	5630	0.11207285412334544	No Hit
TGAGTAGGGGGAGCAAATCGTGCCTTGTCATTTTATTTGGAGGTTTCCTG	5393	0.10735504481122593	No Hit
TAACCAGAGTTAGTAGTGTATGTAAAATTCTGACTGTTCTTGCAACAATA	5302	0.10554356528631928	No Hit
CATGGGCTCGGCCACGCGCTACCACACCTACCTGCCGCCGCCCTACCCCG	5299	0.10548384618110256	No Hit
GAGAACTGGTAGGAGCCGGCCGAGGCGCCGTAGTACAGGTGGTAGGAGGG	5287	0.10524496976023576	No Hit
ATTCTTACTTGTTTGTTTGTTTGTTTGTTTGTTTTTTGTAGGGTACAGGC	5269	0.10488665512893554	No Hit
CATGGAGGAAGTAGGCAGCTGACAGAAAGTGCTGCAATTGCCTGTGTCAA	5172	0.10295573739359548	No Hit
GACTGAACAAGTAGGAAAATGACAGAGTTATCTTCCCAATTGATGTAAGT	5153	0.1025775163938897	No Hit
CATGACTATAAATATAAAGCAAATATGATTATGCTCACAAGCTCTGGAAT	5118	0.10188079349969484	No Hit
GCACGTGGGAGAGTAAGCAGGCCAGGTAGAGCTGCAGAAAACTAAGGCCC	5115	0.10182107439447813	No Hit
AATCTCTTTCCTTTATAAATTACCCAGTCTTGCATATGTCTTTATTAGCA	5035	0.10022856492203273	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	2951550	3.2986438	6.4989195	2
TTTTT	2747405	3.2850664	4.3333297	60-64
GGGGG	650575	3.0862398	8.796453	4
GGAGG	777645	2.7627668	5.7070756	4
CCTGG	752105	2.6236832	5.7925043	9
CCAGC	771910	2.6147714	8.605456	5
CCTCC	755365	2.5526578	8.876618	6
CCCCA	762950	2.5436924	5.569209	1
CAGCC	746430	2.5284605	6.1020637	6
TGGGG	676085	2.4346213	11.646407	3
GCCTG	649995	2.267477	5.0723763	35-39
GCTGG	627045	2.222441	6.5038276	7
GAGGG	599480	2.129794	6.3553286	9
GGGGA	592255	2.1041255	5.6469564	7
CCACC	630825	2.1031847	5.1040707	1
GCCAG	567245	1.952254	7.585178	4
AAGAA	1307805	1.9516307	5.314755	2
TCCAG	743900	1.9434711	5.8505855	4
CACAC	765240	1.9413122	5.055437	8
CCCAC	578310	1.9280984	6.762509	9
TGGCT	709665	1.9093379	6.023689	3
TGCAG	711590	1.8888261	6.6429634	3
CACCA	744080	1.8876319	5.2768865	8
GCCCC	421620	1.8769777	5.018855	9
TGTTT	1164265	1.8338991	5.456089	3
TCCTC	690605	1.799956	5.250024	5
GCCCA	530510	1.7970523	5.7292995	4
GGCTC	506180	1.7657853	6.574425	4
AGGGG	488795	1.7365593	7.471237	6
CCAGA	663825	1.7109996	5.1026926	8
GGCCC	357430	1.6166927	5.0651193	1
TGCCC	445430	1.5293742	10.758215	3
GATGG	559205	1.5081055	5.408403	1
ATGGA	746235	1.5071862	10.714824	2
GCTCC	436780	1.4996747	6.565073	2
CATGG	563320	1.4952619	43.220734	1
TAAAA	1306240	1.4797062	8.169955	1
GCAGT	514025	1.3644147	6.4176216	4
GGGGT	369530	1.3306991	5.445116	4
TGCTC	494845	1.3103882	5.2920117	1
ATGTG	634440	1.2988204	7.6339145	2
TGGGT	468910	1.2817917	6.4480915	3
ATGGC	467585	1.2411454	10.545813	2
ATGAA	816795	1.2354778	8.90383	2
TGAGA	606385	1.2247285	5.3810787	1
ATGGG	454075	1.2245831	14.912448	2
ATGCC	465670	1.2165831	10.931818	2
AGACA	618155	1.2123357	5.0548983	2
CATGT	543525	1.0951648	32.316128	1
GAGAC	416375	1.0903841	5.136779	2
CATGA	534740	1.0630051	29.976377	1
ATGTT	669060	1.0397321	7.833106	2
ATGCT	506605	1.0207735	7.64147	2
TAAAG	674515	1.0202663	6.900324	1
ATGGT	496555	1.0165433	7.5508122	2
ATGAC	495095	0.9841952	7.0334277	2
ATGAT	624680	0.957738	6.589146	2
CATGC	356545	0.9314893	32.558403	1
ATGTA	605845	0.92886084	8.791729	2
GCGCC	192035	0.86859405	5.3439636	8
ATGCA	383645	0.76264465	9.3522415	2
TAACT	498865	0.75278956	5.6053166	1
TAAGT	485265	0.7439918	6.5935717	1
TAAAC	468785	0.6979062	5.5287094	1
TAAGG	331480	0.6694971	5.7891364	1
TGCCG	156005	0.54421616	7.782484	5
CCGTC	124530	0.42757106	5.2859054	7
GCCGT	120250	0.41948652	5.2956386	6
>>END_MODULE