FastQCFastQC Report
Wed 20 Mar 2013
8016-8-21.h.sapiens.aligned.bam

Summary

[OK] Basic Statistics

Measure Value
Filename 8016-8-21.h.sapiens.aligned.bam
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 5023518
Filtered Sequences 0
Sequence length 1-100
%GC 43

[WARN] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
TAAAGAAACCCACAGAAACACACACACATACAATTAGCAAATGAATTCAA 11875 0.2363881248161149 No Hit
AAAAATAATAAACCGTTCATTTCTCAGGATGTGGTCATAGAATAAAGTTA 11826 0.2354127127642421 No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA 9858 0.19623697974208515 No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC 9820 0.19548053774267357 No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC 8944 0.1780425590193964 No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG 8907 0.17730602338839038 No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT 8769 0.17455894454842202 No Hit
CATGGGGTTGTGCCAATTAATTACCAAACATTGAGCCTGCAGGCTTTGAG 8666 0.1725085886026486 No Hit
GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG 8662 0.1724289631290263 No Hit
AAGACAGGGGAAAGGAAGAAAAGGGAGTGAAGAGGGAATGGGAGAGGGAA 8429 0.16779077929052907 No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG 7898 0.15722049766717267 No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA 7825 0.15576733277356625 No Hit
GATACAACATTCAAGACAGAGGAACTGGATGCCAAGCCAGACACAGACCC 7696 0.15319941124924805 No Hit
CATGTATTTTGAACCGGCACCCCTGTTACCACAGAGTGTGGGAGGAACTG 7672 0.15272165840751442 No Hit
CATGTAATTCAGGCAGTTATGGGAGCCCTAGAGGGCCTGAGAGTTGCTATTGGAC 7660 0.1524827819866476 No Hit
GTCCAATAGCAACTCTCAGGCCCTCTAGGGCTCCCATAACTGCCTGAATTACATG 7621 0.15170643361883046 No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA 7472 0.1487403847264009 No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA 7412 0.14754600262206685 No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG 7372 0.14674974788584416 No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC 7362 0.1465506842017885 No Hit
AGCAACAAGAACAATACAAACCTCATTTCCTTTAGTCCTTCTGCCTCTAT 7016 0.1396630807334621 No Hit
TAACTGTGGAAAAACTAAGTTGTCACCAGCGGTACCTCATAGCATAACTT 7000 0.139344578838973 No Hit
ACACTTACACCAAGGAATCTCCCAGGCATTATCAAAGCTACAGGAGACAA 6813 0.13562208794713188 No Hit
CATGTAGACTTACCTTTCCTCATAGAGCTATCCTGGTTAATAACAGGCCA 6800 0.1353633051578595 No Hit
TGAGACCGCACCAGCGACACA 6682 0.1330143536860025 No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT 6628 0.13193940979210186 No Hit
CTTGGGGGAGTGTGCCTCCAGCAGAGAAGTAATTCTGGCCTGGCAACCTA 6623 0.13183987795007404 No Hit
ACCTATGTTACACCATCTTCAGTGCCAGTCTTGGGCAAAATTGTGCAAGA 6617 0.13172043973964065 No Hit
AGGACAACAGCTGATCCATACATTTGCTGACAGGTGTACCTGCGTTTCCC 6616 0.13170053337123505 No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT 6565 0.13068530858255112 No Hit
TGTGTCGCTGGTGCGGTCTCA 6564 0.13066540221414555 No Hit
TAAAACTGCATCAAGTCATGGGGCATGTGGAAGGTAGGGAGGCAAGATGA 6492 0.12923214368894467 No Hit
CATGGAATATATATTCTCCTGGCTGGTGCTTATGATTGCAAAACTTGGAT 6414 0.1276794469533104 No Hit
CACACTGTCATTAGTTGCAGCATCTCCTGTAGCAATTGGGCTAAATCTGT 6346 0.1263258139017318 No Hit
ATTGTTTCAAAAAAAATCAAACTGTAGTTGTTTTGGCGATAGGTCTCACG 6294 0.1252906827446423 No Hit
AACTTTATAAACCAACTTCTGCTAGCTTCCAACTTCTCTTCTGCAGCTTC 6176 0.12294173127278532 No Hit
TAATCCAGAGCAAGGCCTTGACTCCCTTCAGTTCTATGAAGAGAATGAGG 6169 0.12280238669394636 No Hit
ATCCTTCACAAAGTCTTTGAGATATATTTTTATCAAATATTTAGCATGGATCCCGGTACACTTTCAATACTTA 6134 0.12210566379975149 No Hit
* 6089 0.12120987722150094 No Hit
TAAGTATTGAAAGTGTACCGGGATCCATGCTAAATATTTGATAAAAATATATCTCAAAGACTTTGTGAAGGAT 6084 0.1211103453794731 No Hit
CACCAAAAACCCCAAGACAGAAATCTTAGGTATTCAGTTTCTTTTTCACA 5972 0.11888083211804953 No Hit
TAATCTACCTGAAGTCAATGAATGCAATTTTTCACACACACACACACACA 5948 0.11840307927631591 No Hit
TTAGCTTTGAAGTGGATCCTACCAGTTTTATCATCTTTTCACATAAAAGT 5902 0.11748738632965981 No Hit
CACTCAGTATGAGGAGCCGGCTGTCATCACATAGGGACAGGGCTCTCCCT 5761 0.11468058838447479 No Hit
CATGGGTATTATTTCTTTGCTTTTTTTGTGTGGTGGGGGGCTTTATTTGC 5737 0.11420283554274117 No Hit
GAAGCAGTAGTTTCACTTCTAGCTGGTCTCCCTCTGCAGCCTGAAGAAGG 5703 0.11352601901695188 No Hit
CATGCCCTCCAGCGGCCCCGGGGACACCAGCAGCTCTGCTGCGGAGCGGG 5630 0.11207285412334544 No Hit
TGAGTAGGGGGAGCAAATCGTGCCTTGTCATTTTATTTGGAGGTTTCCTG 5393 0.10735504481122593 No Hit
TAACCAGAGTTAGTAGTGTATGTAAAATTCTGACTGTTCTTGCAACAATA 5302 0.10554356528631928 No Hit
CATGGGCTCGGCCACGCGCTACCACACCTACCTGCCGCCGCCCTACCCCG 5299 0.10548384618110256 No Hit
GAGAACTGGTAGGAGCCGGCCGAGGCGCCGTAGTACAGGTGGTAGGAGGG 5287 0.10524496976023576 No Hit
ATTCTTACTTGTTTGTTTGTTTGTTTGTTTGTTTTTTGTAGGGTACAGGC 5269 0.10488665512893554 No Hit
CATGGAGGAAGTAGGCAGCTGACAGAAAGTGCTGCAATTGCCTGTGTCAA 5172 0.10295573739359548 No Hit
GACTGAACAAGTAGGAAAATGACAGAGTTATCTTCCCAATTGATGTAAGT 5153 0.1025775163938897 No Hit
CATGACTATAAATATAAAGCAAATATGATTATGCTCACAAGCTCTGGAAT 5118 0.10188079349969484 No Hit
GCACGTGGGAGAGTAAGCAGGCCAGGTAGAGCTGCAGAAAACTAAGGCCC 5115 0.10182107439447813 No Hit
AATCTCTTTCCTTTATAAATTACCCAGTCTTGCATATGTCTTTATTAGCA 5035 0.10022856492203273 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 2951550 3.2986438 6.4989195 2
TTTTT 2747405 3.2850664 4.3333297 60-64
GGGGG 650575 3.0862398 8.796453 4
GGAGG 777645 2.7627668 5.7070756 4
CCTGG 752105 2.6236832 5.7925043 9
CCAGC 771910 2.6147714 8.605456 5
CCTCC 755365 2.5526578 8.876618 6
CCCCA 762950 2.5436924 5.569209 1
CAGCC 746430 2.5284605 6.1020637 6
TGGGG 676085 2.4346213 11.646407 3
GCCTG 649995 2.267477 5.0723763 35-39
GCTGG 627045 2.222441 6.5038276 7
GAGGG 599480 2.129794 6.3553286 9
GGGGA 592255 2.1041255 5.6469564 7
CCACC 630825 2.1031847 5.1040707 1
GCCAG 567245 1.952254 7.585178 4
AAGAA 1307805 1.9516307 5.314755 2
TCCAG 743900 1.9434711 5.8505855 4
CACAC 765240 1.9413122 5.055437 8
CCCAC 578310 1.9280984 6.762509 9
TGGCT 709665 1.9093379 6.023689 3
TGCAG 711590 1.8888261 6.6429634 3
CACCA 744080 1.8876319 5.2768865 8
GCCCC 421620 1.8769777 5.018855 9
TGTTT 1164265 1.8338991 5.456089 3
TCCTC 690605 1.799956 5.250024 5
GCCCA 530510 1.7970523 5.7292995 4
GGCTC 506180 1.7657853 6.574425 4
AGGGG 488795 1.7365593 7.471237 6
CCAGA 663825 1.7109996 5.1026926 8
GGCCC 357430 1.6166927 5.0651193 1
TGCCC 445430 1.5293742 10.758215 3
GATGG 559205 1.5081055 5.408403 1
ATGGA 746235 1.5071862 10.714824 2
GCTCC 436780 1.4996747 6.565073 2
CATGG 563320 1.4952619 43.220734 1
TAAAA 1306240 1.4797062 8.169955 1
GCAGT 514025 1.3644147 6.4176216 4
GGGGT 369530 1.3306991 5.445116 4
TGCTC 494845 1.3103882 5.2920117 1
ATGTG 634440 1.2988204 7.6339145 2
TGGGT 468910 1.2817917 6.4480915 3
ATGGC 467585 1.2411454 10.545813 2
ATGAA 816795 1.2354778 8.90383 2
TGAGA 606385 1.2247285 5.3810787 1
ATGGG 454075 1.2245831 14.912448 2
ATGCC 465670 1.2165831 10.931818 2
AGACA 618155 1.2123357 5.0548983 2
CATGT 543525 1.0951648 32.316128 1
GAGAC 416375 1.0903841 5.136779 2
CATGA 534740 1.0630051 29.976377 1
ATGTT 669060 1.0397321 7.833106 2
ATGCT 506605 1.0207735 7.64147 2
TAAAG 674515 1.0202663 6.900324 1
ATGGT 496555 1.0165433 7.5508122 2
ATGAC 495095 0.9841952 7.0334277 2
ATGAT 624680 0.957738 6.589146 2
CATGC 356545 0.9314893 32.558403 1
ATGTA 605845 0.92886084 8.791729 2
GCGCC 192035 0.86859405 5.3439636 8
ATGCA 383645 0.76264465 9.3522415 2
TAACT 498865 0.75278956 5.6053166 1
TAAGT 485265 0.7439918 6.5935717 1
TAAAC 468785 0.6979062 5.5287094 1
TAAGG 331480 0.6694971 5.7891364 1
TGCCG 156005 0.54421616 7.782484 5
CCGTC 124530 0.42757106 5.2859054 7
GCCGT 120250 0.41948652 5.2956386 6
Produced by FastQC (version 0.10.0)