##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	8016-8-22.h.sapiens.aligned.bam	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	6347270	
Filtered Sequences	0	
Sequence length	1-100	
%GC	43	
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.94213717078366	24.0	24.0	31.0	23.0	31.0
2	31.009179855806043	31.0	31.0	32.0	29.0	33.0
3	32.647078056439206	33.0	33.0	34.0	29.0	35.0
4	33.867905614914896	34.0	33.0	36.0	29.0	37.0
5	35.39612911013265	36.0	35.0	37.0	33.0	37.0
6	35.724683724361775	36.0	36.0	38.0	33.0	38.0
7	35.741617471176234	37.0	35.0	37.0	34.0	37.0
8	35.59201783164896	36.0	36.0	37.0	32.0	38.0
9	35.613123414795794	37.0	36.0	37.0	31.0	38.0
10-14	35.971977862768874	37.0	36.0	37.4	32.4	38.0
15-19	36.95680001521779	38.0	37.2	38.4	32.6	39.4
20-24	36.5964046333477	37.4	36.0	38.8	32.8	39.8
25-29	36.2512337140139	37.6	36.2	38.0	32.8	38.2
30-34	36.089561130180066	37.6	35.6	38.2	32.4	38.6
35-39	36.12611595725329	37.8	36.4	38.0	31.6	38.6
40-44	36.056124674414164	37.4	36.0	38.4	32.4	38.8
45-49	36.01432678616063	37.6	35.8	38.2	32.0	38.6
50-54	36.22235518091429	37.6	36.2	38.2	33.0	39.0
55-59	35.935906650022346	37.6	35.8	38.2	32.4	38.6
60-64	35.942299401528054	37.6	35.6	38.2	32.6	38.6
65-69	35.745747122289366	37.4	36.0	38.2	31.4	38.6
70-74	35.78088820928499	37.4	36.0	38.4	31.6	39.0
75-79	35.495731874047955	37.4	35.4	37.6	31.6	38.4
80-84	35.60338513446096	37.2	35.8	38.2	31.0	38.6
85-89	35.3275551720091	37.2	35.4	37.6	31.0	38.2
90-94	35.344156161334794	37.0	35.0	38.2	30.8	38.4
95-99	35.171674158316264	37.4	35.2	37.6	30.8	38.2
100	34.09880797549873	36.0	33.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
1	20.0
2	70.0
3	362.0
4	0.0
5	30.0
6	50.0
7	187.0
8	271.0
9	14246.0
10	715.0
11	1001.0
12	1628.0
13	3189.0
14	3553.0
15	4403.0
16	5403.0
17	6541.0
18	8166.0
19	9689.0
20	11124.0
21	11769.0
22	12866.0
23	14938.0
24	18291.0
25	23736.0
26	31467.0
27	42605.0
28	58547.0
29	76659.0
30	99584.0
31	128496.0
32	166603.0
33	224788.0
34	332155.0
35	596816.0
36	1791540.0
37	2645756.0
38	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.071855431332743	19.542099801377955	21.332257417837564	40.05378734945174
2	17.304770468858568	52.65775186776247	15.548073115482602	14.489404547896365
3	18.438698233357318	35.46302402409754	30.608164512824725	15.490113229720412
4	33.86658532216121	25.18252752880679	21.307626616082263	19.64326053294974
5	20.279956505842986	28.004372167657905	26.769288524197492	24.946382802301613
6	19.816321314892445	29.67079166882013	26.520575935585544	23.992311080701885
7	21.9856571063719	28.521253455597307	27.55064163121116	21.942447806819636
8	21.09334258323847	27.920905086134994	29.206116768663954	21.77963556196258
9	20.990245062899668	29.369854979301284	27.48452578971777	22.155374168081273
10-14	21.776858918477114	27.94600167973627	27.210566596532132	23.066572805254484
15-19	21.044792793454256	27.701587508552773	27.890584006425733	23.363035691567234
20-24	21.382800738020308	27.148856708700787	28.15767237593045	23.31067017734846
25-29	21.54038516044448	27.486746602906077	28.600424884318947	22.372443352330492
30-34	22.05665000079166	27.873831396780325	27.878606994641906	22.19091160778611
35-39	21.95899165719434	26.748025786813002	29.105695237924646	22.187287318068016
40-44	22.084669493631615	27.89090984724623	27.6970522803347	22.327368378787458
45-49	21.6812221602873	28.484394880188784	28.339436889822846	21.494946069701072
50-54	22.14430510936189	27.885809627445724	28.022508958491343	21.947376304701038
55-59	21.65642622318141	28.660094747806085	27.7542191847534	21.929259844259104
60-64	21.94033575448396	28.156457010231755	28.55162041936778	21.351586815916505
65-69	21.972698214845128	29.026497527299718	28.185718265084382	20.815085992770772
70-74	21.79316763478836	28.631704697117925	27.797363028523804	21.777764639569913
75-79	22.36898537733783	28.59370019553566	27.796838851971263	21.240475575155255
80-84	21.62069228373042	28.56759470675559	27.998712740940633	21.81300026857335
85-89	21.751998217466383	28.271271862190588	28.302615650699497	21.674114269643535
90-94	21.688425452010083	28.03857517079881	28.40362609849376	21.869373278697342
95-99	21.779830309734482	27.421528636526034	28.648607734192122	22.15003331954736
100	23.186038860119027	27.284144734380796	27.198954005397802	22.330862400102376
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	59.12564278078448
2	31.794175016754934
3	33.92881146307773
4	53.509845855110946
5	45.226339308144595
6	43.80863239559433
7	43.92810491319153
8	42.87297814520105
9	43.14561923098094
10-14	44.8434317237316
15-19	44.4078284850215
20-24	44.69347091536876
25-29	43.91282851277498
30-34	44.24756160857777
35-39	44.14627897526236
40-44	44.41203787241907
45-49	43.17616822998837
50-54	44.091681414062926
55-59	43.58568606744051
60-64	43.291922570400466
65-69	42.7877842076159
70-74	43.57093227435827
75-79	43.60946095249309
80-84	43.43369255230377
85-89	43.42611248710992
90-94	43.557798730707425
95-99	43.92986362928185
100	45.5169012602214
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	15586.0
1	15585.5
2	15588.0
3	15592.5
4	15599.0
5	15613.0
6	15643.5
7	15672.5
8	15684.5
9	15689.0
10	15724.0
11	15781.0
12	15807.0
13	15860.5
14	15952.5
15	16698.5
16	17216.5
17	17073.0
18	17629.0
19	18739.0
20	20013.5
21	22208.0
22	27415.5
23	35733.5
24	51168.5
25	64606.0
26	81947.5
27	101378.5
28	107392.0
29	128537.5
30	162423.5
31	176525.0
32	183024.5
33	199977.5
34	221588.0
35	222605.0
36	213327.5
37	204130.5
38	227654.5
39	251595.5
40	249082.5
41	261421.0
42	251238.5
43	252918.0
44	262698.5
45	243581.5
46	220123.0
47	195014.0
48	182579.0
49	197834.5
50	206470.5
51	206597.0
52	175271.5
53	113305.5
54	101234.0
55	104886.0
56	84452.0
57	72879.0
58	92408.5
59	90979.0
60	90201.5
61	112731.0
62	102379.5
63	90052.5
64	81488.5
65	71040.5
66	68949.5
67	62876.0
68	54110.0
69	52373.5
70	50126.5
71	43363.0
72	47486.5
73	33697.0
74	14910.0
75	17958.0
76	18349.5
77	17656.5
78	15146.0
79	12469.5
80	10747.0
81	9807.5
82	8473.5
83	8264.0
84	8030.5
85	6564.0
86	9187.0
87	8871.5
88	6246.0
89	6307.0
90	6533.5
91	6892.0
92	5622.0
93	4310.0
94	4216.0
95	4197.0
96	4187.0
97	4182.5
98	4183.5
99	4183.0
100	4176.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.052888879786112765
2	0.007912851861304922
3	0.0019743929136669057
4	0.007392493712431363
5	0.005103129830100535
6	7.744493665004182E-4
7	3.3213480117936324E-4
8	2.372576057670681E-4
9	4.745232425438558E-5
10-14	0.001436246262200738
15-19	0.019181115100549605
20-24	0.01748558999186097
25-29	0.00816501678083719
30-34	0.007816625992329322
35-39	0.005171271152376849
40-44	0.013297549200932042
45-49	0.011568245012852925
50-54	0.01049482467691949
55-59	0.010682299354993832
60-64	0.054809457453212015
65-69	0.044671314043204374
70-74	0.028494243943076206
75-79	0.07463382778244239
80-84	0.06538500127864753
85-89	0.015295928674785735
90-94	0.006718988003199219
95-99	0.009189802961517493
100	0.007237104319187766
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
0-1	15798.0
2-3	726.0
4-5	3670.0
6-7	4834.0
8-9	117.0
10-11	55.0
12-13	146.0
14-15	425.0
16-17	491.0
18-19	4180.0
20-21	48751.0
22-23	5670.0
24-25	9643.0
26-27	3622.0
28-29	3482.0
30-31	5634.0
32-33	3986.0
34-35	3839.0
36-37	3240.0
38-39	2942.0
40-41	4235.0
42-43	3307.0
44-45	4406.0
46-47	9373.0
48-49	2935.0
50-51	22349.0
52-53	4862.0
54-55	25730.0
56-57	19797.0
58-59	49558.0
60-61	3645.0
62-63	12119.0
64-65	7430.0
66-67	9083.0
68-69	7353.0
70-71	32849.0
72-73	26770.0
74-75	17584.0
76-77	20688.0
78-79	7907.0
80-81	13907.0
82-83	18947.0
84-85	9300.0
86-87	3788.0
88-89	7562.0
90-91	15469.0
92-93	2602.0
94-95	5136.0
96-97	136808.0
98-99	0.0
100-101	5720520.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	97.1493062408277
#Duplication Level	Relative count
1	100.0
2	6.73209815997809
3	2.071855753491838
4	1.1406989408250483
5	0.8630078530530098
6	0.8114184537192135
7	0.875745976345305
8	0.8891210058022152
9	1.0037641154328734
10++	12.993522664305868
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
*	13800	0.21741630653808647	No Hit
AAAAATAATAAACCGTTCATTTCTCAGGATGTGGTCATAGAATAAAGTTA	12720	0.20040111733075797	No Hit
TAAAGAAACCCACAGAAACACACACACATACAATTAGCAAATGAATTCAA	12712	0.20027507889218515	No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA	12605	0.1985893147762739	No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC	12561	0.19789610336412347	No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT	11799	0.1858909420900639	No Hit
GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG	11744	0.18502442782487588	No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC	11390	0.1794472269180293	No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG	11277	0.17766693397318847	No Hit
CACTCAGTATGAGGAGCCGGCTGTCATCACATAGGGACAGGGCTCTCCCT	10186	0.16047844191282237	No Hit
CATGCCCTCCAGCGGCCCCGGGGACACCAGCAGCTCTGCTGCGGAGCGGG	10159	0.16005306218263915	No Hit
CATGGGGTTGTGCCAATTAATTACCAAACATTGAGCCTGCAGGCTTTGAG	9986	0.15732748094850227	No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG	9960	0.15691785602314065	No Hit
TGAGACCGCACCAGCGACACA	9715	0.15305792884184855	No Hit
TGTGTCGCTGGTGCGGTCTCA	9614	0.1514666935548669	No Hit
AAGACAGGGGAAAGGAAGAAAAGGGAGTGAAGAGGGAATGGGAGAGGGAA	9584	0.15099404941021888	No Hit
GAGACCGCACCAGCGACACC	9283	0.14625185315891714	No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG	9175	0.14455033423818428	No Hit
GATACAACATTCAAGACAGAGGAACTGGATGCCAAGCCAGACACAGACCC	9117	0.14363655555853147	No Hit
CATGTATTTTGAACCGGCACCCCTGTTACCACAGAGTGTGGGAGGAACTG	9091	0.14322693063316985	No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA	9062	0.14277004129334345	No Hit
GGTGTCGCTGGTGCGGTCTC	9017	0.14206107507637142	No Hit
CATGTAATTCAGGCAGTTATGGGAGCCCTAGAGGGCCTGAGAGTTGCTATTGGAC	8106	0.1277084478838934	No Hit
GTCCAATAGCAACTCTCAGGCCCTCTAGGGCTCCCATAACTGCCTGAATTACATG	8041	0.12668438557048936	No Hit
CTTGGGGGAGTGTGCCTCCAGCAGAGAAGTAATTCTGGCCTGGCAACCTA	7924	0.12484107340636211	No Hit
AGGACAACAGCTGATCCATACATTTGCTGACAGGTGTACCTGCGTTTCCC	7870	0.12399031394599568	No Hit
CATGTAGACTTACCTTTCCTCATAGAGCTATCCTGGTTAATAACAGGCCA	7704	0.12137501634561	No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT	7696	0.1212489779070372	No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT	7653	0.12057152129970837	No Hit
ACACTTACACCAAGGAATCTCCCAGGCATTATCAAAGCTACAGGAGACAA	7649	0.12050850208042198	No Hit
GAGAACTGGTAGGAGCCGGCCGAGGCGCCGTAGTACAGGTGGTAGGAGGG	7609	0.11987830988755795	No Hit
TAACTGTGGAAAAACTAAGTTGTCACCAGCGGTACCTCATAGCATAACTT	7565	0.11918509847540754	No Hit
AGCAACAAGAACAATACAAACCTCATTTCCTTTAGTCCTTCTGCCTCTAT	7562	0.11913783406094272	No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA	7419	0.11688489697145386	No Hit
GACGCGACCGGACCGAGCGCCAAACGCGGCAGCCCAATCGCCATCGCTTT	7397	0.11653829126537865	No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC	7381	0.11628621438823306	No Hit
CACCAAAAACCCCAAGACAGAAATCTTAGGTATTCAGTTTCTTTTTCACA	7089	0.11168581138032571	No Hit
CGAGCCCGGCGGGCCCTGTGATTGGACGGGCGCCCGCCTCGCGTCCCGCC	7041	0.11092958074888888	No Hit
ACCTATGTTACACCATCTTCAGTGCCAGTCTTGGGCAAAATTGTGCAAGA	6947	0.10944862909565846	No Hit
TAAAACTGCATCAAGTCATGGGGCATGTGGAAGGTAGGGAGGCAAGATGA	6911	0.10888145612208083	No Hit
TAATCTACCTGAAGTCAATGAATGCAATTTTTCACACACACACACACACA	6608	0.10410775026113589	No Hit
ACAGATTTTGGGGTTGTGTTGTCACCCAAGAGATTGTTGTTTGCCATACT	6509	0.10254802458379744	No Hit
GCCTCAGCCTTCCTTCGCAGAAAGTTGAGCAGGTCGCCATAGCAACAGTA	6485	0.10216990926807903	No Hit
CATGGAGGGGAGCACTCAGCACCTCCCTCACCCCACACCCTTGGCTGCTC	6476	0.10202811602468462	No Hit
AACTTTATAAACCAACTTCTGCTAGCTTCCAACTTCTCTTCTGCAGCTTC	6461	0.10179179395236061	No Hit
TAATCCAGAGCAAGGCCTTGACTCCCTTCAGTTCTATGAAGAGAATGAGG	6443	0.1015082074655718	No Hit
CATGGGTATTATTTCTTTGCTTTTTTTGTGTGGTGGGGGGCTTTATTTGC	6402	0.1008622604678862	No Hit
GAAGCAGTAGTTTCACTTCTAGCTGGTCTCCCTCTGCAGCCTGAAGAAGG	6398	0.10079924124859979	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	3658100	3.4550054	6.362892	2
TTTTT	3342475	3.4046745	4.38024	60-64
GGGGG	895450	3.1217668	8.963015	4
CCCAG	1175040	3.004221	4.346629	7
GGAGG	1022475	2.7452292	5.062085	4
CCAGC	1044800	2.6712368	9.216906	5
CCTGG	981555	2.5896373	5.9012003	9
CCTCC	1011090	2.5819604	9.236376	6
CCCCA	1019050	2.563259	5.7155294	1
CAGCC	995385	2.5448973	6.624971	6
TGGGG	884385	2.4106276	11.5896	3
CTCCC	901170	2.3012643	5.0347867	3
GCTGG	839570	2.2514558	8.67886	7
GAGGG	791415	2.1248593	6.3107176	9
GGGGA	777680	2.0879824	5.279841	7
AAGAA	1593025	1.9536598	5.2010403	2
TCCAG	947110	1.924383	5.9509187	4
GCCAG	739520	1.9218141	7.3953094	4
CCCAC	762470	1.9178725	6.095506	9
TGGCT	905735	1.899054	6.138534	3
CACCA	960650	1.8915211	6.518621	8
TGGTG	873160	1.8608538	6.0162234	9
TGTTT	1422330	1.8530114	5.4625397	3
GCCCA	712135	1.8207133	5.3472934	4
TGCAG	878395	1.8141081	6.148074	3
GGCTC	682285	1.800073	6.9740887	1
TCCTC	884750	1.7955283	5.880545	5
AGGGG	630765	1.6935323	6.6231766	6
CCAGA	839825	1.680804	5.0383167	8
TGCCC	597825	1.5517298	11.572346	3
GCTCC	588260	1.5269027	7.097428	2
TAAAA	1590940	1.5254917	7.928255	1
GATGG	715010	1.5009551	5.413686	1
CTGGT	713945	1.496928	5.428154	8
ATGGA	899305	1.4538829	9.977287	2
CATGG	697590	1.4407002	41.49562	1
GCAGT	633440	1.3082142	5.9145555	4
ATGTG	795240	1.3052192	7.576537	2
TGCTC	621605	1.2822373	5.203959	1
TGGGT	601365	1.281612	5.8873267	3
ATGAA	995285	1.2391866	8.802507	2
ATGGC	592920	1.2245301	10.763666	2
ATGGG	580705	1.219021	14.835485	2
TGAGA	751760	1.2153506	5.613774	1
ATGCC	591020	1.2008625	12.579111	2
GAGAC	552935	1.1248239	5.610616	2
CATGT	686210	1.1080518	32.694767	1
ATGTT	837020	1.0741149	8.226774	2
CATGA	670135	1.0658656	29.218246	1
GTGTC	499010	1.046274	5.7215037	2
TAAAG	830000	1.0333974	6.448368	1
ATGCT	624615	1.0085918	7.7595754	2
ATGGT	607570	0.99719846	7.27506	2
CACTC	488980	0.9774621	5.458456	1
AGACC	485795	0.9722575	5.337412	3
ATGAC	601975	0.95745564	6.8419905	2
ATGAT	754885	0.9541856	6.438855	2
ATGTA	745380	0.94217104	9.070213	2
CATGC	459745	0.9341318	33.169487	1
GCGCC	264920	0.8794824	5.1139574	8
CGCTG	314815	0.83057666	6.636831	6
TAAGA	652525	0.81243086	5.0938873	1
TAAGT	597865	0.75571007	6.2574577	1
ATGCA	470735	0.7487152	9.086257	2
TAACT	591875	0.73603725	5.2787566	1
TAAAC	561475	0.68776083	5.230273	1
TGCCG	213525	0.5633431	6.4337907	5
GTCGC	131745	0.3475829	6.050659	4
>>END_MODULE