FastQCFastQC Report
Wed 20 Mar 2013
8016-8-22.h.sapiens.aligned.bam

Summary

[OK] Basic Statistics

Measure Value
Filename 8016-8-22.h.sapiens.aligned.bam
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 6347270
Filtered Sequences 0
Sequence length 1-100
%GC 43

[WARN] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
* 13800 0.21741630653808647 No Hit
AAAAATAATAAACCGTTCATTTCTCAGGATGTGGTCATAGAATAAAGTTA 12720 0.20040111733075797 No Hit
TAAAGAAACCCACAGAAACACACACACATACAATTAGCAAATGAATTCAA 12712 0.20027507889218515 No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA 12605 0.1985893147762739 No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC 12561 0.19789610336412347 No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT 11799 0.1858909420900639 No Hit
GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG 11744 0.18502442782487588 No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC 11390 0.1794472269180293 No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG 11277 0.17766693397318847 No Hit
CACTCAGTATGAGGAGCCGGCTGTCATCACATAGGGACAGGGCTCTCCCT 10186 0.16047844191282237 No Hit
CATGCCCTCCAGCGGCCCCGGGGACACCAGCAGCTCTGCTGCGGAGCGGG 10159 0.16005306218263915 No Hit
CATGGGGTTGTGCCAATTAATTACCAAACATTGAGCCTGCAGGCTTTGAG 9986 0.15732748094850227 No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG 9960 0.15691785602314065 No Hit
TGAGACCGCACCAGCGACACA 9715 0.15305792884184855 No Hit
TGTGTCGCTGGTGCGGTCTCA 9614 0.1514666935548669 No Hit
AAGACAGGGGAAAGGAAGAAAAGGGAGTGAAGAGGGAATGGGAGAGGGAA 9584 0.15099404941021888 No Hit
GAGACCGCACCAGCGACACC 9283 0.14625185315891714 No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG 9175 0.14455033423818428 No Hit
GATACAACATTCAAGACAGAGGAACTGGATGCCAAGCCAGACACAGACCC 9117 0.14363655555853147 No Hit
CATGTATTTTGAACCGGCACCCCTGTTACCACAGAGTGTGGGAGGAACTG 9091 0.14322693063316985 No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA 9062 0.14277004129334345 No Hit
GGTGTCGCTGGTGCGGTCTC 9017 0.14206107507637142 No Hit
CATGTAATTCAGGCAGTTATGGGAGCCCTAGAGGGCCTGAGAGTTGCTATTGGAC 8106 0.1277084478838934 No Hit
GTCCAATAGCAACTCTCAGGCCCTCTAGGGCTCCCATAACTGCCTGAATTACATG 8041 0.12668438557048936 No Hit
CTTGGGGGAGTGTGCCTCCAGCAGAGAAGTAATTCTGGCCTGGCAACCTA 7924 0.12484107340636211 No Hit
AGGACAACAGCTGATCCATACATTTGCTGACAGGTGTACCTGCGTTTCCC 7870 0.12399031394599568 No Hit
CATGTAGACTTACCTTTCCTCATAGAGCTATCCTGGTTAATAACAGGCCA 7704 0.12137501634561 No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT 7696 0.1212489779070372 No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT 7653 0.12057152129970837 No Hit
ACACTTACACCAAGGAATCTCCCAGGCATTATCAAAGCTACAGGAGACAA 7649 0.12050850208042198 No Hit
GAGAACTGGTAGGAGCCGGCCGAGGCGCCGTAGTACAGGTGGTAGGAGGG 7609 0.11987830988755795 No Hit
TAACTGTGGAAAAACTAAGTTGTCACCAGCGGTACCTCATAGCATAACTT 7565 0.11918509847540754 No Hit
AGCAACAAGAACAATACAAACCTCATTTCCTTTAGTCCTTCTGCCTCTAT 7562 0.11913783406094272 No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA 7419 0.11688489697145386 No Hit
GACGCGACCGGACCGAGCGCCAAACGCGGCAGCCCAATCGCCATCGCTTT 7397 0.11653829126537865 No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC 7381 0.11628621438823306 No Hit
CACCAAAAACCCCAAGACAGAAATCTTAGGTATTCAGTTTCTTTTTCACA 7089 0.11168581138032571 No Hit
CGAGCCCGGCGGGCCCTGTGATTGGACGGGCGCCCGCCTCGCGTCCCGCC 7041 0.11092958074888888 No Hit
ACCTATGTTACACCATCTTCAGTGCCAGTCTTGGGCAAAATTGTGCAAGA 6947 0.10944862909565846 No Hit
TAAAACTGCATCAAGTCATGGGGCATGTGGAAGGTAGGGAGGCAAGATGA 6911 0.10888145612208083 No Hit
TAATCTACCTGAAGTCAATGAATGCAATTTTTCACACACACACACACACA 6608 0.10410775026113589 No Hit
ACAGATTTTGGGGTTGTGTTGTCACCCAAGAGATTGTTGTTTGCCATACT 6509 0.10254802458379744 No Hit
GCCTCAGCCTTCCTTCGCAGAAAGTTGAGCAGGTCGCCATAGCAACAGTA 6485 0.10216990926807903 No Hit
CATGGAGGGGAGCACTCAGCACCTCCCTCACCCCACACCCTTGGCTGCTC 6476 0.10202811602468462 No Hit
AACTTTATAAACCAACTTCTGCTAGCTTCCAACTTCTCTTCTGCAGCTTC 6461 0.10179179395236061 No Hit
TAATCCAGAGCAAGGCCTTGACTCCCTTCAGTTCTATGAAGAGAATGAGG 6443 0.1015082074655718 No Hit
CATGGGTATTATTTCTTTGCTTTTTTTGTGTGGTGGGGGGCTTTATTTGC 6402 0.1008622604678862 No Hit
GAAGCAGTAGTTTCACTTCTAGCTGGTCTCCCTCTGCAGCCTGAAGAAGG 6398 0.10079924124859979 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 3658100 3.4550054 6.362892 2
TTTTT 3342475 3.4046745 4.38024 60-64
GGGGG 895450 3.1217668 8.963015 4
CCCAG 1175040 3.004221 4.346629 7
GGAGG 1022475 2.7452292 5.062085 4
CCAGC 1044800 2.6712368 9.216906 5
CCTGG 981555 2.5896373 5.9012003 9
CCTCC 1011090 2.5819604 9.236376 6
CCCCA 1019050 2.563259 5.7155294 1
CAGCC 995385 2.5448973 6.624971 6
TGGGG 884385 2.4106276 11.5896 3
CTCCC 901170 2.3012643 5.0347867 3
GCTGG 839570 2.2514558 8.67886 7
GAGGG 791415 2.1248593 6.3107176 9
GGGGA 777680 2.0879824 5.279841 7
AAGAA 1593025 1.9536598 5.2010403 2
TCCAG 947110 1.924383 5.9509187 4
GCCAG 739520 1.9218141 7.3953094 4
CCCAC 762470 1.9178725 6.095506 9
TGGCT 905735 1.899054 6.138534 3
CACCA 960650 1.8915211 6.518621 8
TGGTG 873160 1.8608538 6.0162234 9
TGTTT 1422330 1.8530114 5.4625397 3
GCCCA 712135 1.8207133 5.3472934 4
TGCAG 878395 1.8141081 6.148074 3
GGCTC 682285 1.800073 6.9740887 1
TCCTC 884750 1.7955283 5.880545 5
AGGGG 630765 1.6935323 6.6231766 6
CCAGA 839825 1.680804 5.0383167 8
TGCCC 597825 1.5517298 11.572346 3
GCTCC 588260 1.5269027 7.097428 2
TAAAA 1590940 1.5254917 7.928255 1
GATGG 715010 1.5009551 5.413686 1
CTGGT 713945 1.496928 5.428154 8
ATGGA 899305 1.4538829 9.977287 2
CATGG 697590 1.4407002 41.49562 1
GCAGT 633440 1.3082142 5.9145555 4
ATGTG 795240 1.3052192 7.576537 2
TGCTC 621605 1.2822373 5.203959 1
TGGGT 601365 1.281612 5.8873267 3
ATGAA 995285 1.2391866 8.802507 2
ATGGC 592920 1.2245301 10.763666 2
ATGGG 580705 1.219021 14.835485 2
TGAGA 751760 1.2153506 5.613774 1
ATGCC 591020 1.2008625 12.579111 2
GAGAC 552935 1.1248239 5.610616 2
CATGT 686210 1.1080518 32.694767 1
ATGTT 837020 1.0741149 8.226774 2
CATGA 670135 1.0658656 29.218246 1
GTGTC 499010 1.046274 5.7215037 2
TAAAG 830000 1.0333974 6.448368 1
ATGCT 624615 1.0085918 7.7595754 2
ATGGT 607570 0.99719846 7.27506 2
CACTC 488980 0.9774621 5.458456 1
AGACC 485795 0.9722575 5.337412 3
ATGAC 601975 0.95745564 6.8419905 2
ATGAT 754885 0.9541856 6.438855 2
ATGTA 745380 0.94217104 9.070213 2
CATGC 459745 0.9341318 33.169487 1
GCGCC 264920 0.8794824 5.1139574 8
CGCTG 314815 0.83057666 6.636831 6
TAAGA 652525 0.81243086 5.0938873 1
TAAGT 597865 0.75571007 6.2574577 1
ATGCA 470735 0.7487152 9.086257 2
TAACT 591875 0.73603725 5.2787566 1
TAAAC 561475 0.68776083 5.230273 1
TGCCG 213525 0.5633431 6.4337907 5
GTCGC 131745 0.3475829 6.050659 4
Produced by FastQC (version 0.10.0)