##FastQC 0.10.0 >>Basic Statistics pass #Measure Value Filename 8016-8-23.h.sapiens.aligned.bam File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6017304 Filtered Sequences 0 Sequence length 1-100 %GC 38 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.95746766325916 24.0 24.0 31.0 23.0 31.0 2 31.01175124838717 31.0 31.0 32.0 27.0 33.0 3 32.62578998155428 33.0 33.0 34.0 29.0 35.0 4 33.8783892991686 34.0 33.0 36.0 29.0 37.0 5 35.38623132120505 36.0 35.0 37.0 33.0 37.0 6 35.718905566616776 36.0 36.0 38.0 33.0 38.0 7 35.77894712830621 37.0 35.0 37.0 34.0 37.0 8 35.608734438324234 36.0 36.0 37.0 32.0 38.0 9 35.60475855354497 37.0 36.0 37.0 31.0 38.0 10-14 35.990007168251374 37.0 36.0 37.4 32.4 38.0 15-19 36.9922705240124 38.0 37.2 38.6 33.4 39.4 20-24 36.685035143080256 37.4 36.2 38.8 32.8 39.8 25-29 36.346284340511986 37.6 36.2 38.0 32.8 38.2 30-34 36.227458322563486 37.6 35.6 38.2 32.8 38.6 35-39 36.19334063442973 37.8 36.4 38.0 31.6 38.6 40-44 36.139910411875995 37.6 36.0 38.4 32.4 38.8 45-49 36.11352542828688 37.6 36.0 38.2 32.6 38.6 50-54 36.398814560064935 37.6 36.4 38.6 33.6 39.0 55-59 36.13460054910757 37.6 35.8 38.2 33.0 38.6 60-64 36.18030065424634 37.6 36.0 38.2 33.2 38.6 65-69 35.962563235996036 37.4 36.0 38.2 32.4 38.6 70-74 36.00033061635528 37.4 36.0 38.4 32.0 39.0 75-79 35.709587379350396 37.4 36.0 37.6 32.4 38.4 80-84 35.87797753975623 37.2 36.0 38.2 32.6 38.6 85-89 35.5610416677557 37.2 35.8 37.8 31.6 38.2 90-94 35.57558182201492 37.0 35.0 38.2 32.8 38.4 95-99 35.4823242296592 37.4 35.4 37.6 32.0 38.2 100 34.24855802532616 36.0 33.0 38.0 28.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 1 1.0 2 8.0 3 35.0 4 0.0 5 3.0 6 16.0 7 100.0 8 269.0 9 5466.0 10 594.0 11 835.0 12 1090.0 13 1687.0 14 2233.0 15 2857.0 16 3511.0 17 4249.0 18 5210.0 19 6779.0 20 8496.0 21 9527.0 22 10312.0 23 12084.0 24 15378.0 25 20294.0 26 26701.0 27 36873.0 28 51569.0 29 70857.0 30 94800.0 31 123402.0 32 159488.0 33 213159.0 34 312197.0 35 549601.0 36 1653889.0 37 2613726.0 38 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 14.60448370731954 25.97256495138257 29.917010542263917 29.505940799033976 2 18.213601367652398 54.943675164922254 16.748002859201303 10.094720608224046 3 15.036799026943367 41.84599302811311 31.715381600686143 11.401826344257374 4 32.932589129747626 28.11361027466769 21.65120031941972 17.302600276164966 5 17.4044936734032 33.839717339512916 32.040374269216045 16.715414717867848 6 15.537767179314088 32.355971239018324 29.172271303361384 22.933990278306204 7 24.01343218769897 31.3669855101807 28.43317340096492 16.186408901155403 8 16.836720844982562 30.38645659226314 31.033678837151484 21.743143725602813 9 14.884476613070735 33.32142917226826 34.25844877565648 17.535645439004526 10-14 19.70811015186002 29.316720750035763 27.882470004573335 23.09269909353089 15-19 19.288212612344644 29.807424739837064 30.948126958458584 19.956235689359715 20-24 20.363767298340623 29.29230462434706 30.133761124843023 20.210166952469294 25-29 19.714165302012653 29.2370275147136 32.1866607941064 18.862146389167343 30-34 19.551857480111675 30.120048219189314 30.41402300568438 19.914071295014626 35-39 20.488950083523243 29.67131758275338 29.400012736682267 20.439719597041105 40-44 19.25548345245345 30.171540339549598 30.916584551976868 19.656391656020087 45-49 19.52122295804305 31.294816411717708 31.159392164475243 18.024568465764002 50-54 20.116480475366323 30.601383578947583 31.208255919087907 18.073880026598193 55-59 19.181922523583587 32.199195134023235 29.98084393972946 18.638038402663717 60-64 18.782764499620956 30.848070293504605 32.2181141909822 18.151051015892243 65-69 19.483259972246497 33.67646396529946 27.950541344651647 18.889734717802394 70-74 19.30417112464675 30.608111494031604 29.170426414420124 20.91729096690152 75-79 19.23993513160707 32.49597713363723 29.946813212321043 18.317274522434648 80-84 18.45503347930405 31.76307196016418 31.766807173181714 18.01508738735006 85-89 19.750740169991538 30.598681411561024 29.580932289474486 20.069646128972956 90-94 18.43111232415589 29.79374447969856 31.279041997884754 20.496101198260796 95-99 17.411842625619602 29.444520808296442 33.259682317843414 19.88395424824054 100 18.654598311400473 35.7308580160487 27.959609233122833 17.654934439427993 >>END_MODULE >>Per base GC content fail #Base %GC 1 44.11042450635352 2 28.308321975876442 3 26.438625371200747 4 50.23518940591259 5 34.119908391271046 6 38.47175745762029 7 40.19984108885438 8 38.57986457058538 9 32.420122052075264 10-14 42.80080924539091 15-19 39.24444830170436 20-24 40.57393425080992 25-29 38.576311691179995 30-34 39.4659287751263 35-39 40.92866968056435 40-44 38.91187510847354 45-49 37.54579142380705 50-54 38.19036050196451 55-59 37.81996092624731 60-64 36.9338155155132 65-69 38.3729946900489 70-74 40.22146209154827 75-79 37.55720965404172 80-84 36.470120866654106 85-89 39.820386298964486 90-94 38.927213522416686 95-99 37.29579687386014 100 36.309532750828474 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5365.0 1 5360.5 2 5358.5 3 5385.5 4 5428.0 5 5482.5 6 5544.0 7 5606.5 8 5684.5 9 5779.5 10 5885.5 11 5988.0 12 6039.5 13 6158.5 14 6315.5 15 6929.5 16 7621.0 17 9152.0 18 9728.5 19 11222.0 20 12233.0 21 11554.0 22 28496.0 23 41059.5 24 126775.5 25 203210.5 26 136255.5 27 170395.0 28 197076.0 29 140963.5 30 185851.0 31 258768.0 32 252171.5 33 286681.0 34 306824.0 35 275404.5 36 233919.5 37 156817.0 38 217308.0 39 368911.0 40 359326.0 41 299455.5 42 245961.5 43 181251.5 44 183837.5 45 170599.0 46 164587.0 47 142054.5 48 127551.5 49 122821.0 50 117470.0 51 122283.0 52 110042.5 53 82943.5 54 80229.0 55 97798.0 56 131746.0 57 144268.0 58 98791.0 59 54907.5 60 49327.5 61 55631.0 62 75164.5 63 88777.0 64 79723.0 65 50150.5 66 24346.0 67 21199.0 68 16819.0 69 14012.0 70 13426.5 71 11988.0 72 11029.0 73 9028.5 74 7277.0 75 9828.5 76 11083.0 77 8409.5 78 6433.0 79 6492.0 80 5755.0 81 5352.0 82 6319.5 83 6227.5 84 5066.0 85 4023.0 86 3349.0 87 2623.0 88 1998.5 89 1753.0 90 1379.0 91 1037.0 92 751.5 93 473.5 94 388.5 95 307.0 96 294.0 97 317.0 98 340.5 99 342.0 100 340.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0551908296472972 2 0.00811715719190939 3 0.0019794763237165718 4 0.007419303430546171 5 0.004742289038024173 6 0.001015231974681113 7 4.3290778498069726E-4 8 1.665263127394128E-4 9 3.3313872479493225E-5 10-14 0.001386748068778304 15-19 0.017902319733159245 20-24 0.01612635667079105 25-29 0.008671564122198647 30-34 0.008203792040177203 35-39 0.005665306655245517 40-44 0.013382710921907267 45-49 0.012559987955549924 50-54 0.011541849102684116 55-59 0.011979457614522224 60-64 0.05776134804457869 65-69 0.045924313531506264 70-74 0.02899705715000133 75-79 0.07740237379484483 80-84 0.06934442562055705 85-89 0.015845088420136776 90-94 0.007618857012248165 95-99 0.01029270242023626 100 0.008855984333880752 >>END_MODULE >>Sequence Length Distribution warn #Length Count 0-1 5347.0 2-3 611.0 4-5 2867.0 6-7 3422.0 8-9 2464.0 10-11 3114.0 12-13 2918.0 14-15 6012.0 16-17 5163.0 18-19 6479.0 20-21 7845.0 22-23 9547.0 24-25 9455.0 26-27 8342.0 28-29 7136.0 30-31 4174.0 32-33 7359.0 34-35 2530.0 36-37 3668.0 38-39 11987.0 40-41 101717.0 42-43 104139.0 44-45 82566.0 46-47 37971.0 48-49 2372.0 50-51 16008.0 52-53 1899.0 54-55 18434.0 56-57 11150.0 58-59 19450.0 60-61 2167.0 62-63 2806.0 64-65 4424.0 66-67 3273.0 68-69 2247.0 70-71 22678.0 72-73 45574.0 74-75 7837.0 76-77 114776.0 78-79 4593.0 80-81 7172.0 82-83 8504.0 84-85 2145.0 86-87 729.0 88-89 1952.0 90-91 5286.0 92-93 1494.0 94-95 2083.0 96-97 144941.0 98-99 0.0 100-101 5126477.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Duplicate Percentage 97.64628575815924 #Duplication Level Relative count 1 100.0 2 11.161477088187532 3 5.278542681635247 4 3.216126526865777 5 2.2827084657205394 6 1.7771658546918025 7 1.3860057897338134 8 1.1777165854691802 9 0.9828426180893879 10++ 12.71058391583704 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ACCTATGTTACACCATCTTCAGTGCCAGTCTTGGGCAAAATTGTGCAAGA 83215 1.3829283014452984 No Hit TAAAACTGCATCAAGTCATGGGGCATGTGGAAGGTAGGGAGGCAAGATGA 81671 1.3572689696249352 No Hit CACCAAAAACCCCAAGACAGAAATCTTAGGTATTCAGTTTCTTTTTCACA 77960 1.2955968320696445 No Hit ATACTTGTAATTCCCCTAACCATAAGATTTACTGCTGCTGTGGATATCTC 74115 1.2316977835921203 No Hit TAAAAATTTACAGATTGTGCTGAGCTTGACAAATAAGTGTATCCTTATAT 73715 1.2250502883018706 No Hit CATGGTCACTCTCCCCAAAATATTATATTTTTTCTATAAAAAGAAAAAAA 68435 1.1373033504705763 No Hit TAAATATAGCCCCAAAATGGTTGCTATAATAATCCCCATTTCATACTGGG 68048 1.1308718987772597 No Hit CTGAGGAAATAAGATGTAGGGCATTTCTGATGTGACTCAGTGGGAAAACT 60812 1.0106187089766447 No Hit AAAGAAGTCATCTCAAACTCTTAGTTTTTTTTTTTTACAACTATGTGATT 59865 0.9948807638769788 No Hit AAGGAATAAACTTGATTATATTGTTTTTTTATTTGGCATAACTGTGATTC 52554 0.8733811687094419 No Hit TAATGTGTACAGTAATTGTCCTAAAAGAATCACAGTTATGCCAAATAAAA 52047 0.8649554684290507 No Hit AAAATGACAGTGGAAGTTTTTTTTTCCTCTAAGTGCCAGTATTCCCAGAG 40524 0.6734577478551856 No Hit AGTTATGATTTTGCAGAAAACAGATCTGTATTTATTTCAGTGTTACTTAC 36574 0.607813731863971 No Hit GTAGACACAAAACAGGCTCAGGACTTAGCAAGAAGTTATGGAATTCCTTT 36190 0.6014321363853313 No Hit ATTTTCACACAGCCAGGAGTCTTTTCTTCTTTGCTGATTTTTTTCAATCT 35989 0.5980917700019809 No Hit TAAAGAAGTTGTGTTTTACAATGCAGAGAGTGGAGGATGCTTTTTATACA 35160 0.5843148360129387 No Hit TAAAAAAATGCCCATCTACATCAAAAATTCAAGAGGCCTAAATATCCCCT 32884 0.5464905878114186 No Hit AGACTTACACAGTACCTCGTTTCTACACAGAGAAAGAAATGGCCATACTT 31588 0.5249527030710099 No Hit TAACATGCCCCACAAAGTTTCTATGTATATATTAGGACAAAATTGTGCAA 31058 0.5161447718114291 No Hit ACTGAGTCACACTGCATAGGAATTTAGAACCTAACTTTTATAGGTTATCA 31025 0.5155963534499836 No Hit GATTTCTGTCTTGGGGTTTTTGGTGCATGCAGTTGATTACTTCTTATTTT 30097 0.5001741643766046 No Hit TAGATAAAACACAGAATAGGGATGATTCAAAAGCTTCATTAATTTGTTTC 30054 0.4994595586329027 No Hit TGAAGATTTCCATTTGTCAAAAAGTAATGATTTCTTGATAATTGTGTAGT 24741 0.4111642024401626 No Hit TAAGGTAAAAGCTAACAGTCTGCATGGAGCAGGAAAAAAATTAGGTAATG 24724 0.4108816838903269 No Hit ACCTTATGTGTGACATGTTCTAATATAGTCACATTTTCATTATTTTTATT 23939 0.39783597438321217 No Hit TAAAACAAGATTTACCTCTATTGTTGGATCATATTCGTCCACAAAATGAT 23876 0.39678899387499783 No Hit CTATCCAGTATTAACACAGAAGTTACTAAATATAAATTCAGCTTTAAGGT 23752 0.39472827033502045 No Hit CATGCTACTAAATTTTTATAATAATTGAAAAGATTTTAACAAGTATAAAA 23373 0.388429768547509 No Hit TGTGTCGCTGGTGCGGTCTCACTGCAGCCTGCAGAGCCACA 23272 0.38675127598672093 No Hit AAAGTTATACTATGAAAGAGCAGTCTGACACAGGGAGACTACATTTAATT 22966 0.3816659420896801 No Hit AGAATCATTTTGTGGACGAATATGATCCAACAATAGAGGTAAATCTTGTT 22907 0.3806854365343682 No Hit GAGAAACCTTTATCTGTATCAAAGAATGGTCCTGCACCAGTAATATGCAT 22734 0.37781039482133527 No Hit TGTGGCTCTGCAGGCTGCAGTGAGACCGCACCAGCGACACA 22605 0.3756665775902298 No Hit CTTATTATATTCAATTTAAACCCACCTATAATGGTGAATATCTTCAAATG 20531 0.3411993145102857 No Hit CATGCAGACTGTTAGCTTTTACCTTAAATGCTTATTTTAAAATGACAGTG 20333 0.33790880434161213 No Hit GTGCAATGAGGGACCAGTACATGAGGACTGGGGAGGGCTTTCTTTGTGTA 20173 0.33524980622551226 No Hit CAGAACTCATTTATTCAGCAAATATTTATTGAGTGCCTACCAGATGCCAG 19364 0.32180524700098245 No Hit CCTTAGTAAGATATTACAGACCACACTAGCACTACCTAAGGACCGGGATT 19319 0.3210574037808294 No Hit AGGCATACTAGTACAAGTGGTAATTTTTGTACATTACACTAAATTATTAG 19152 0.31828207449715024 No Hit TGTGTCGCTGGTGCGGTCTCAGCTTGGCGCAATCATGACACA 18003 0.2991871442759083 No Hit TGTGTCATGATTGCGCCAAGCTGAGACCGCACCAGCGACACA 17700 0.2941516665935442 No Hit TAAGAAATAGTACTAGTAAGAAATTGGCACTCAAAGGAAAAATGCAAAAG 17501 0.29084453768664503 No Hit GGCCCATCTCTCCCCCCACACCCCCACAGAGCTAACTGGGTTACAGTGTT 17270 0.2870056091565259 No Hit ATGCCTAGAAGAATCATCATCAGGAAGCCCATAAATTTGTGTTCCCTCAA 16398 0.2725140694237818 No Hit CATGCAGTTGATTACTTCTTATTTTTCTTACCAATTGTGAATGTTGGTGT 16393 0.2724309757326537 No Hit CAGACTGTTAGCTTTTACCTTAAATGCTTATTTTAAAATGACAGTGGAAG 16222 0.26958917149607203 No Hit TGTGTCGCTGGTGCGGTCTCACGAGACTGCCCTTAACCTACACA 16195 0.2691404655639802 No Hit CATGTTCTAATATAGTCACATTTTCATTATTTTTATTATAAGGCCTGCTG 16075 0.26714621697690527 No Hit TGTGTAGGTTAAGGGCAGTCTCGTGAGACCGCACCAGCGACACA 15933 0.26478635614886664 No Hit AAAGATTATTTGGGCCAGTTATAGCTTATTAGGTGTTGAAGAGACCAAGG 15628 0.2597176409900514 No Hit GGATCTCTCTCACCAATGTATAAAAAGCATCCTCCACTCTCTGCATTGTA 15433 0.2564769870360547 No Hit AAATAAATGAAGTGAGATGGCATGGTGAGGTGAAAGTATCACTGGACTAG 15232 0.25313662065270426 No Hit GCTTTCCCAGTAAATTACTCTTACCAATGCAACAGACTTTAAAGAAGTTG 15186 0.25237215869432555 No Hit TAAATTCTCCTTCCACTGGATAGGGTTCTGTCTATTCATACCAGGTTTGA 15087 0.2507269036099888 No Hit AATGACATAACAGTTATGATTTTGCAGAAAACAGATCTGTATTTATTTCA 14984 0.24901517357274952 No Hit TAACGTGAAGAAATGGATAGTAAGTGATGTCCTCAAAATCAGAGTCCTAA 14941 0.24830056782904772 No Hit ATACTGGATAGCATGAATTCTGCATTGAGAAACTGAATAGCTGTCATAAA 14752 0.24515962630440477 No Hit TAATCTAGTTATGACTATTCTTCAAGAACTCATGTGAGTATCTTTCTTTA 14583 0.2423510595442743 No Hit TAACACCCTACCTAAACAGTGTTCCAAAGATCTGTAGTTTCACATAGCAA 14553 0.2418524973975056 No Hit ATAGTTTTATCTGGGTACAAATAAACAGGTGCCTGAACTAGTTCACAGAC 14514 0.24120436660670627 No Hit CTACTTTATGTAAATCACTTCATTGTTTTAAAGGAATAAACTTGATTATATTGTTTTTTTATTTGGCATAAC 13492 0.22422001614011855 No Hit GTTATGCCAAATAAAAAAACAATATAATCAAGTTTATTCCTTTAAAACAATGAAGTGATTTACATAAAGTAG 13306 0.2211289308301525 No Hit CATGTACTGGTCCCTCATTGCACTGTACTCCTCTTGACCTGCTGTGTCGA 13229 0.21984928798677947 No Hit AAATTGAATTTTTTGTTGTTGAGTTGTATATAACACCTTTTTTGAAGTAA 12962 0.21541208488053787 No Hit CATGAGTTCTTGAAGAATAGTCATAACTAGATTAAGATCTGTGTTTTAGT 12955 0.21529575371295848 No Hit AGTAGGATTTTTCAAACCTGGTATGAATAGACAGAACCCTATCCAGTGGA 12641 0.21007746991011256 No Hit TAACAGGAAAAGCTATTAGGAGTCTTTATAGTAATTTATCTAATGTGAAA 12585 0.20914682056947764 No Hit GTTACCAGGAGTAGTCCTAGTTATAGATTACCTAAGGAATTCTTTCAGCA 12505 0.2078173215114277 No Hit CCCCACAAAGTTTCTATGTATATATTAGGACAAAATTGTGCAATGGTGAC 12133 0.20163515089149558 No Hit CATGTTTGTGGAAGAATATAGCAGACGTATATTGTATCATTTGAGTGAAT 12129 0.2015686759385931 No Hit TTAGCAAGAAGTTATGGAATTCCTTTTATTGAAACATCAGCAAAGACAAG 11617 0.19305988196707363 No Hit TAAGAAACAAAAGCAATGCTCTTGATTTGTCAGCAGGACCACCACAGAGT 11530 0.19161405174144433 No Hit GAATTAGCTGTATCGTCAAGGCACTCTTGCCTACGCCACCAGCTCCAACT 11513 0.19133153319160873 No Hit GTATAGCATAATTGAGAGAAAAACTGATATATTAAATGACATAACAGTTA 11398 0.18942037829566197 No Hit CAAAAGTATTTTCAACATGAAAACACAAGACAGTGGAATTGGAAACTTTC 10358 0.17213689054101305 No Hit CCCATCTCTCCCCCCACACCCCCACAGAGCTAACTGGGTTACAGTGTTTT 10250 0.17034206681264566 No Hit TAATTTGTTTCACACCAACATTCACAATTGGTAAGAAAAATAAGAAGTAA 10237 0.17012602321571255 No Hit ACTATTTTTGTATAGTGTAAACTGAAACATGCACATTTTGTACGTTGTGC 10132 0.16838105570202203 No Hit CTGTGAAACTCTCTATGAAAGCTCAAAGGTTCACACAGGGCCTGGCCTTG 9427 0.15666484525295715 No Hit TGAGCCTCTGGCCCCGCCGCCGCCTTCAGTGCCTGCGCCGCGCTCGCTCC 8994 0.14946893160126196 No Hit AACCCACCTATAATGGTGAATATCTTCAAATGATTTAGTATTATTTATGG 8723 0.14496525354211787 No Hit AGTTACAGTTTGCACAAGTTCATCTCATTTGTATTCCATTGATTTTTTTT 8489 0.14107646879732186 No Hit TAAGGTAACTGCTGGGTTCTAAAAAACATTACTACACAATTATCAAGAAA 8398 0.13956416361879007 No Hit AGATATTCATATTGACCCAAATGTGTAATATTCCAGTTTTCTCTGCATAA 8367 0.13904898273379573 No Hit GAAATCATAGTGATTTTTACATAGAAGTTTCCTTGTCTGTGAACTAGTTC 8274 0.1375034400788127 No Hit GTGTAACTTTACATTCATCAGGGATGACAAACTATAGGACATGATGCCTA 8226 0.13670574064398278 No Hit GGCGGCGGCCGCGGCGGCGGAGGCAGCAGCGGCGGCGGCAGTGGCGGCGG 8168 0.13574185382689657 No Hit CATTTTTTAATTTTCACACAGCCAGGAGTCTTTTCTTCTTTGCTGATTTT 8109 0.13476134827158476 No Hit CAGAGAGTGGAGGATGCTTTTTATACATTGGTGAGAGAGATCCGACAATA 7894 0.13118831955307558 No Hit AAATGACAGTGGAAGTTTTTTTTTCCTCTAAGTGCCAGTATTCCCAGAGT 7779 0.12927716465712882 No Hit ATTACTAATTTCAGTTGAGACCTTCTAATTGGTTTTTACTGAAACATTGA 7765 0.1290445023219701 No Hit CTTTTGAATCATCCCTATTCTGTGTTTTATCTAGTCACATAAATGGATTA 7706 0.1280639967666583 No Hit AAAACACAAGACAGTGGAATTGGAAACTTTCGGATAAAACACTGTAACCC 7703 0.1280141405519814 No Hit TAAAGAAACCCACAGAAACACACACACATACAATTAGCAAATGAATTCAA 7654 0.12719982237892585 No Hit AAGATTTCTGGTTACTAAAACAATGGAATGTATTACTGTTACCAGGAGTA 7647 0.1270834912113465 No Hit TAAGCAAACACGCCTTTACATAATATCCACAGCACCTTTTAGAAATAAAG 7620 0.12663478527925465 No Hit TAAGGACTCTGAAGATGTACCTATGGTCCTAGTAGGAAATAAATGTGATT 7617 0.12658492906457774 No Hit AAAAATAATAAACCGTTCATTTCTCAGGATGTGGTCATAGAATAAAGTTA 7612 0.12650183537344964 No Hit AATGTTTATAGGAGTATGTGCTGTGAAGTGATCTAAAATTTGTAATATTT 7570 0.12580384836797343 No Hit TAACTGTGGAAAAACTAAGTTGTCACCAGCGGTACCTCATAGCATAACTT 7502 0.124673774168631 No Hit AGCAACAAGAACAATACAAACCTCATTTCCTTTAGTCCTTCTGCCTCTAT 7492 0.12450758678637476 No Hit TAGTCCAGTGATACTTTCACCTCACCATGCCATCTCACTTCATTTATTTT 7459 0.12395916842492917 No Hit GTTCTCTTAACACTGGTTAAATTAACATTGCATAAACACTTTTCAAGTCT 7426 0.12341075006348358 No Hit TAGAACAGTAGACACAAAACAGGCTCAGGACTTAGCAAGAAGTTATGGAA 7418 0.12327780015767859 No Hit GAGCAGGAAAAAAATTAGGTAATGCTAAAACAAATGCTAATAATTTAGTG 7121 0.11834203490466827 No Hit CAAGAGGTGAAGTTTATATTTGAATATCCATTCTCGTTTTAGGACTCTTC 7087 0.11777699780499706 No Hit CATGAAGCCATCGTATATATTCACATTTTAATACTTTTTATGTATTTCAG 7031 0.11684634846436211 No Hit CATCAAGTCATGGGGCATGTGGAAGGTAGGGAGGCAAGATGACACTAATA 6923 0.11505152473599471 No Hit CATGTAGACTTACCTTTCCTCATAGAGCTATCCTGGTTAATAACAGGCCA 6904 0.11473576870970788 No Hit ACACTTACACCAAGGAATCTCCCAGGCATTATCAAAGCTACAGGAGACAA 6870 0.11417073161003666 No Hit GTGTTTCTCCCTTCTCAGGATTCCTACAGGAAGCAAGTAGTAATTGATGG 6869 0.11415411287181101 No Hit TGTACTCCTCTTGACCTGCTGTGTCGAGAATATCCAAGAGACAGGTTTCT 6856 0.1139380692748779 No Hit AGACTGCTCTTTCATAGTATAACTTTAAATCTTTTCTTCAACTTGAGTCT 6778 0.11264180769327925 No Hit GATTAAGATCTGTGTTTTAGTTTAATAGTTTGAAGTGCCTGTTTGGGATA 6721 0.11169453961441868 No Hit GATACAACATTCAAGACAGAGGAACTGGATGCCAAGCCAGACACAGACCC 6709 0.11149511475571121 No Hit TAGTAAGAAATTGGCACTCAAAGGAAAAATGCAAAAGTATTTTCAACATG 6681 0.11102979008539374 No Hit CATGTATTTTGAACCGGCACCCCTGTTACCACAGAGTGTGGGAGGAACTG 6639 0.11033180307991751 No Hit TAATGTTTGATATGACCAACATTCCTAGGTCAGCGCAACCAAATGATGGA 6249 0.10385049517192416 No Hit ATCCTTCACAAAGTCTTTGAGATATATTTTTATCAAATATTTAGCATGGATCCCGGTACACTTTCAATACTTA 6191 0.10288660835483798 No Hit CATTTTTCCTTTGAGTGCCAATTTCTTACTAGTACTATTTCTTAATGTAA 6150 0.1022052400875874 No Hit TAAGTATTGAAAGTGTACCGGGATCCATGCTAAATATTTGATAAAAATATATCTCAAAGACTTTGTGAAGGAT 6148 0.10217200261113615 No Hit CATGTTTCAGTTTACACTATACAAAAATAGTTAAAATACATTCCAGGTAA 6081 0.10105854715001936 No Hit >>END_MODULE >>Kmer Content fail #Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position CCCCA 878865 3.6951602 20.527653 10-14 GGGGG 505860 3.4914935 6.5340285 90-94 TTTTT 4609560 3.352548 9.925144 25-29 AAAAA 4837490 3.1155078 9.569458 80-84 CTGGG 707090 3.0951996 9.0661 75-79 TCCCC 707680 3.0486624 14.022368 10-14 CCCAG 699035 2.9547713 7.969007 6 TGCTG 989575 2.7619107 7.5743194 30-34 GGAGG 639005 2.7445474 8.983231 35-39 CACCA 1034275 2.720486 20.536446 1 CAGTG 998720 2.720466 11.442167 8 CCAGC 635080 2.684438 10.907279 30-34 TGGGG 602315 2.6506448 8.110422 15-19 CTCCC 604005 2.6020339 7.5876884 10-14 CCCCC 384110 2.5814712 8.6940775 10-14 GCCAG 607425 2.581258 8.710577 20-24 CACAG 975005 2.5782864 17.711676 8 CTGTG 907985 2.5341923 6.760067 35-39 CAGCA 947720 2.5061347 10.36139 65-69 CTGCA 923970 2.5034769 21.346663 6 GCCTG 572500 2.4927323 7.5038466 70-74 GCTGG 561870 2.4595168 65.84698 7 TGCAG 902340 2.4579313 13.423809 3 TGGTG 867270 2.4334874 40.03769 9 GGGAG 564085 2.4227636 8.704925 35-39 CACTG 886270 2.4013295 7.3416204 60-64 CAGGC 563380 2.3940883 8.301651 45-49 CTGCT 857015 2.3792248 5.5650053 35-39 TGTGG 828055 2.3234534 11.389441 1 GGGGA 538485 2.3128107 7.673064 90-94 CCAGG 537265 2.2831128 8.125336 5 CCCAC 542160 2.2794948 16.009426 9 GCCCC 334385 2.2592914 44.885315 9 TGGAA 1321320 2.2516816 6.1114635 50-54 CCAGT 824315 2.233464 8.172471 5 TGTGT 1233885 2.2074778 36.707264 1 GGGCA 516270 2.2056139 8.477964 30-34 CAGGA 824530 2.192021 5.5323267 4 ACACA 1326615 2.1830053 11.610903 7 GTGCC 498855 2.172073 8.178448 20-24 CTGCC 500980 2.169735 8.046621 50-54 GGAAG 808725 2.1614883 5.5274367 60-64 CCTCC 501565 2.1607258 7.0927334 85-89 TGCCT 771205 2.1410012 5.158435 2 ACTGC 788565 2.1365998 21.342617 5 GTGGA 776200 2.125627 6.1263328 40-44 GGGGC 306305 2.1029122 11.718353 20-24 AGGCA 790005 2.1002357 6.271766 50-54 TTCCC 751110 2.0741334 5.636765 60-64 CCTGG 473735 2.062698 5.0200896 9 CCACA 783475 2.0607991 5.6907115 60-64 ACTGG 748840 2.0398047 6.8680024 85-89 TGCCA 748930 2.0292096 5.2781115 20-24 CCCTC 465350 2.0047126 7.2419615 85-89 TCTCC 717595 1.9815843 6.2955737 6 ACAGG 739715 1.9665391 5.8124013 45-49 AGCAG 739470 1.965888 6.2552958 65-69 TTCCT 1102600 1.951695 5.0649004 90-94 GCTGC 445640 1.9403687 8.295408 30-34 GCAGC 455995 1.9377549 6.773158 65-69 CTGGC 444660 1.9361017 10.297693 85-89 AAAAC 1875080 1.9303194 12.048296 2 AAGAA 1862010 1.9271041 13.441431 2 GGAAA 1153350 1.9182214 10.263035 5 GTGGG 434570 1.912439 6.8878083 40-44 ATGGA 1120145 1.9088562 7.7433925 50-54 GAGGG 441495 1.8962356 11.165205 8 AGGCC 444370 1.8883543 7.023052 85-89 CTCTG 679560 1.8865782 11.244279 6 CACAC 712755 1.8747818 10.244863 6 ACCAG 706060 1.867093 7.6612563 30-34 TTTCC 1054410 1.8663946 5.7815638 7 CTCTC 673910 1.8609515 20.611248 9 TGCCC 429295 1.8592687 16.657078 9 TCTTC 1045785 1.8511276 7.5678387 90-94 GGTGC 421060 1.8431385 12.849112 10-14 GGCGG 267610 1.8372549 6.5148325 1 CAAGA 1109255 1.8350804 5.4301534 6 TCTGC 658805 1.8289587 9.245304 7 GAAGT 1068745 1.8212646 14.9576 4 TTGTG 1012310 1.8110698 6.6025286 9 GGCCA 425745 1.8092072 7.435917 85-89 TCACA 1072645 1.8085365 5.8319197 5 AGGAA 1087270 1.8083189 9.9540615 4 TCCCA 665190 1.792741 5.707807 5 CTTTT 1572330 1.7840146 5.772491 95-96 GGGAA 666650 1.7817631 6.0005174 80-84 CCCCT 412915 1.7788244 7.386642 10-14 CTTCC 642200 1.7733867 13.460383 95-96 CTGAG 650765 1.772653 16.09279 1 AGAAG 1065760 1.772544 14.564077 3 AGTGG 646945 1.7716616 11.433412 9 CAAAA 1719030 1.7696719 8.399532 4 AGAGG 660505 1.7653391 11.582834 95-96 AAATG 1662210 1.7626678 6.263874 2 AGGGG 409480 1.75873 6.8033695 85-89 GCGGC 257525 1.7586224 5.968995 5 CTTCT 993235 1.7581097 5.879356 90-94 TGAAG 1031100 1.7571132 7.396127 50-54 CCCAA 667135 1.7547863 11.271305 10-14 GACAG 659820 1.7541378 12.476303 6 AGGAG 652250 1.7432759 5.1642084 75-79 GCAGT 638040 1.7379906 7.8736167 4 CTCCA 640655 1.7266171 5.4403496 45-49 GGCCC 253760 1.7237033 13.3838625 1 CTGGT 617560 1.723614 42.785324 8 AGTTT 1547455 1.7227619 5.3611283 55-59 TGGCA 630305 1.7169209 6.0154343 85-89 ATGCC 632725 1.7143548 9.090932 8 TGGGC 391630 1.7143122 7.7038016 30-34 TGTGA 976400 1.7048575 5.4250503 40-44 CCAAA 1031890 1.698022 12.471082 3 TTTTG 1485400 1.6943847 5.6385474 9 TCTTT 1490100 1.6907139 5.2810283 80-84 GGCAC 397650 1.6898172 9.133975 85-89 TTCCA 977215 1.6881953 8.718408 95-96 GTCCT 604420 1.6779766 5.244258 65-69 AGGAC 630690 1.6766951 5.8690057 85-89 GGCAA 628250 1.6702086 5.662266 65-69 GACAC 631595 1.6701791 9.350933 4 GCACC 394825 1.668897 9.295501 30-34 TGGGA 607065 1.6624501 5.6067905 75-79 GCAGA 624305 1.6597208 5.4533277 4 CATGG 608600 1.6577975 42.063934 1 AAAGA 1600745 1.6567056 10.105279 1 AGCCC 391765 1.6559628 27.804684 8 GGCCT 379825 1.6538025 5.625154 80-84 GCCAC 386620 1.6342151 5.4763103 35-39 TCTTG 916655 1.6312245 6.897902 9 GTGGC 371015 1.6240726 17.145844 2 GAAGA 974925 1.6214696 6.4176416 60-64 TTCTG 910770 1.6207519 5.9425898 4 GCCCT 374185 1.620588 8.201477 80-84 CTCTT 915210 1.6199988 5.1301374 90-94 GGCTC 371820 1.6189477 15.012573 4 GTGTG 576720 1.6182283 6.881969 8 TGGCC 365800 1.592736 5.6118045 3 TGTTT 1395890 1.5922812 5.4899454 3 GGTGA 581380 1.5921116 9.109431 50-54 CACAA 964270 1.58675 6.1646557 6 TCTGT 890550 1.5847696 5.309844 5 AGGGA 590180 1.5773809 6.2690268 9 GAGGA 589850 1.5764989 15.924182 3 TCTGG 563180 1.5718392 5.9067307 75-79 CTCAG 578940 1.5686255 5.579722 85-89 GGCAT 575475 1.5675666 7.7969728 20-24 TGTGC 561575 1.5673597 7.114177 40-44 GAGTC 574645 1.5653057 9.173622 4 AGGTG 571525 1.5651236 6.871877 50-54 GCCCA 368555 1.5578557 8.83371 2 TTGGG 553975 1.5544077 5.0506444 30-34 GAGGC 361735 1.5454081 8.099485 35-39 AACTG 905850 1.5354702 14.0836525 4 AAAAT 2313510 1.5266571 5.645717 3 AAGGC 573635 1.5250139 5.0927744 85-89 GGAAT 888470 1.5140553 9.0546665 3 AAACT 1431005 1.509426 9.421398 3 GTCAC 555735 1.505752 17.789581 5 TCCAG 553660 1.5001298 10.926673 4 TAAAA 2272175 1.4993805 16.445513 1 CCTTC 541910 1.4964436 5.770154 9 ACTCT 863145 1.4911329 12.719138 8 CCAAG 563020 1.4888406 5.35604 10-14 GAGAC 557985 1.4834082 6.8395534 20-24 CTTGG 529890 1.4789267 5.4702992 30-34 GAGAG 549330 1.4682004 5.692335 55-59 AGGGC 341595 1.4593657 5.6017237 15-19 TGACA 860395 1.4584212 7.831681 5 GAAGG 541990 1.4485828 5.225508 30-34 GTGTC 518445 1.4469836 51.306023 2 TTAGG 828150 1.4460036 5.7040753 80-84 CTGTC 518905 1.4405718 8.333496 6 ATGTG 824900 1.4403288 9.481931 3 GTCTC 517780 1.4374486 9.72685 15-19 ACCCC 340610 1.4320838 31.516657 9 TCTCA 828065 1.4305302 5.6949954 15-19 AAGGA 854820 1.4217141 8.721513 1 GACTG 515955 1.4054371 5.4099483 7 GCAAG 527055 1.4011807 5.385372 45-49 CTCCT 505010 1.3945469 7.2755904 7 GTCTT 782130 1.3918319 5.191348 8 CCTTT 780145 1.3809224 7.692561 95-96 TGGCT 494580 1.380376 10.07791 3 ACAGT 811870 1.3761684 14.0890045 9 GGTCT 488845 1.3643696 8.667093 15-19 CATCT 789520 1.3639414 13.048648 9 GAGCC 319850 1.3592055 5.128892 2 CTTGT 760510 1.3533581 12.512528 4 TGAGG 492870 1.3497266 15.784133 2 GCCGC 198480 1.3482057 5.8431225 8 AGTCA 793430 1.3449116 13.526325 6 TTTGA 1200860 1.3369023 8.7452965 95-96 ACAGC 503535 1.3315393 9.362272 9 CCATC 491500 1.3246324 6.543027 10-14 AGACA 797470 1.3192835 6.0726123 3 AAGTT 1212040 1.3169304 5.133332 55-59 CAGTC 485525 1.3155195 5.3899918 25-29 ACACT 777840 1.3114794 6.264224 9 CATCA 776180 1.3086808 13.407705 9 CCTAA 774505 1.3058566 5.335547 95-96 CACCC 309565 1.3015561 6.3623743 4 ACAAG 780780 1.2916726 5.9546475 5 GCTCC 297805 1.2897879 7.061779 95-96 ACTGA 756815 1.2828469 5.231443 1 TAGGG 467980 1.2815652 5.027365 15-19 CAGCG 300585 1.2773387 8.886515 30-34 ACCAA 775705 1.2764578 12.559933 2 TTCAC 736430 1.2722254 5.9043384 4 TGTCT 705915 1.2562041 6.0561376 7 GTGCT 449885 1.255632 5.2316365 15-19 CACAT 742255 1.2514813 5.1558228 70-74 AGTCC 461810 1.2512642 5.538075 2 GTAGG 455605 1.2476763 10.59246 4 GCACA 470915 1.2452797 5.2729034 90-94 AGGTA 728845 1.2420359 5.9722595 3 GTCTG 443085 1.2366533 5.130474 50-54 ATTTC 1110195 1.2293985 5.0663075 95-96 AATGC 719940 1.2203416 5.7342744 7 GGACT 446030 1.2149645 6.736516 85-89 ATGGT 693835 1.2114807 13.987197 2 ACACC 459935 1.2097814 5.2428145 10-14 CAGTA 707035 1.1984669 5.276778 6 CCTAC 444600 1.1982331 5.2078547 85-89 TTACA 1103140 1.1922371 16.46947 8 TGGTC 426640 1.1907551 17.955795 3 CGCTG 273350 1.1901981 60.81489 6 AAATA 1770425 1.1682819 5.120757 2 CATGC 430020 1.1651299 34.468582 1 GAAAT 1095350 1.1615487 6.5464664 6 ATGGC 426320 1.1612754 5.5970793 2 ATGAC 683950 1.1593363 7.263389 2 GACTC 426895 1.1566628 6.3277946 85-89 GCTCT 416540 1.1563885 8.759951 5 CACTC 427935 1.1533196 18.233343 7 CATGA 680155 1.1529037 22.329014 1 ATCCA 683625 1.1526281 5.368538 3 ACCTA 681940 1.1497871 13.10854 1 AGTTG 650430 1.135693 6.670538 7 AACCC 431565 1.1351591 19.778275 8 AACAC 689385 1.1344144 5.516863 2 AAACC 688835 1.1335094 13.054113 7 CGGCG 165920 1.1330575 5.6842246 3 CAGAC 428235 1.1324173 5.3991866 5 ACTTG 651115 1.1308478 12.420588 3 TGTCA 644090 1.1186469 5.9030237 3 TACAG 658140 1.1155869 13.457174 9 GTGCG 254660 1.1147429 12.977927 10-14 TACAC 653750 1.1022573 13.453302 9 TGCAT 633705 1.1006104 13.4978485 7 GTGCA 403610 1.0994145 5.3168497 1 GAGGT 400610 1.0970721 5.1200395 45-49 GCGGT 250350 1.0958763 13.337503 10-14 CGCAC 259080 1.0951127 8.3701515 25-29 TGCGG 249815 1.0935345 13.452884 10-14 CCGCA 258490 1.0926188 10.239473 25-29 CATGT 628855 1.0921869 28.101295 1 TTTAC 974075 1.078663 8.599716 7 GCATG 395765 1.0780451 6.770065 20-24 ATGGG 391975 1.0734252 7.645593 2 GTTTG 598195 1.0701988 7.2087274 95-96 GTTGT 593310 1.0614593 6.4371886 8 AAATT 1568645 1.0606111 5.2454367 4 GACCG 246385 1.0470154 9.097195 25-29 AATTC 968765 1.0470091 7.688823 9 ATTTA 1501755 1.0403798 5.3276234 6 AAGGT 607950 1.0360168 6.477032 2 GAATA 971760 1.0304894 5.6830387 4 GCTCA 375935 1.0185877 5.025193 85-89 TCATG 585410 1.0167322 5.9589114 5 ACCGC 240500 1.0165763 10.45903 25-29 AACTT 939540 1.0154238 6.0296664 9 CGGTC 232925 1.0141827 13.027664 10-14 GCTTG 363315 1.0140147 5.491454 20-24 TGCTA 582835 1.01226 5.035162 3 CTATG 578905 1.0054344 13.593374 3 GTCAT 577965 1.0038018 14.536024 7 ATGCT 576390 1.0010664 7.976006 2 GGTAA 586955 1.000239 6.2155175 4 GCATC 368715 0.99902534 20.91305 8 ATGAA 939675 0.9964653 5.751348 2 TAAAT 1463635 0.98961025 7.0966115 1 TGAGT 563785 0.98440504 8.697468 3 TCATC 567760 0.9808383 13.020401 8 ATGCA 573110 0.97145593 10.3832445 2 ATGTT 870680 0.96931696 9.163438 5 TAGGT 554760 0.9686468 5.475724 5 AATTT 1395515 0.96677935 5.4685535 5 GGTCC 220370 0.95951676 6.4468303 9 AAGTC 564340 0.95659024 13.458889 5 GGGTC 217435 0.951795 6.4016833 45-49 GTCGC 218450 0.95115685 60.577637 4 AGACT 560805 0.9505982 8.226398 1 GTAAT 873970 0.94960374 7.701336 7 AATGT 870730 0.9460834 6.833683 2 TGTAG 538300 0.93990666 8.431004 3 GGTCA 342285 0.9323681 17.48507 4 TAAGG 545340 0.92932224 9.529959 1 GTGTA 526230 0.9188316 8.58786 5 TCACT 530230 0.91600305 11.842061 6 TGTAA 835445 0.90774477 8.1292 6 TGTAC 518680 0.90083647 9.161309 6 CCTAT 520085 0.89847696 13.401256 2 GTACA 522845 0.8862537 9.507748 7 ATACT 816370 0.8823057 9.465665 1 AGAAC 532425 0.8808099 5.6639614 2 CTTAC 509265 0.8797847 6.9310446 4 GCGAC 202660 0.86120546 8.824774 35-39 TAAGA 807025 0.8557985 5.965624 1 TACTT 772470 0.85541135 9.7169895 2 TGTCG 304835 0.8507966 38.948406 3 ATGTA 775880 0.84302497 6.1031785 2 ATAAG 787000 0.83456326 6.379387 9 GTTAC 475865 0.826476 14.048621 7 TTGTA 732935 0.8159672 8.363805 5 TATGT 732855 0.8158781 8.820846 4 GCGCC 118655 0.8059821 6.262455 8 GACTT 457125 0.79392856 5.6648793 2 CGGCC 114880 0.78033984 6.2317553 6 TAATT 1119825 0.7757879 5.4483194 8 CGACA 291345 0.77042776 6.83587 35-39 TAATG 694650 0.75476533 8.045951 1 GGCCG 109890 0.750432 5.7462373 7 ACATG 440450 0.74658924 5.242313 3 TCGCT 266805 0.74069774 38.622795 5 TAAAG 698050 0.7402375 7.2602363 1 ACCCT 267305 0.7204086 5.621638 5 TAGAG 421390 0.71809715 7.2845116 95-96 TAACA 666895 0.70344174 7.8902154 1 TGTTA 591100 0.65806407 10.387573 6 TATAG 605275 0.6576558 6.9655657 5 CAATC 377840 0.6370583 6.9191346 95-96 CTCGT 220605 0.6124383 5.0028605 75-79 TCTCG 213755 0.5934215 5.0184813 75-79 TAGCC 217730 0.5899347 17.146858 7 TAAAC 554195 0.5845656 5.754929 7 ATAGC 342305 0.58022755 11.613951 6 GTAGA 315730 0.53804034 6.1507854 1 GCTAC 195670 0.53016365 6.715567 4 ATCTA 441210 0.47684518 5.6044946 95-96 CCGCG 62905 0.4272918 5.6547976 9 TGCCG 91460 0.3982276 6.8842 5 TAGAC 228670 0.3876094 5.9994283 2 CCGTC 75625 0.32753047 6.008838 7 GCCGT 71810 0.31266916 6.0457983 6 >>END_MODULE