FastQCFastQC Report
Wed 20 Mar 2013
8016-8-24.h.sapiens.aligned.bam

Summary

[OK] Basic Statistics

Measure Value
Filename 8016-8-24.h.sapiens.aligned.bam
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 6198948
Filtered Sequences 0
Sequence length 1-100
%GC 44

[WARN] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC 14764 0.23816944423473144 No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG 14686 0.23691116621723557 No Hit
TAAAGAAACCCACAGAAACACACACACATACAATTAGCAAATGAATTCAA 13666 0.22045676137305878 No Hit
AAAAATAATAAACCGTTCATTTCTCAGGATGTGGTCATAGAATAAAGTTA 13653 0.2202470483701428 No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC 13348 0.21532685868634482 No Hit
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA 13346 0.2152945951474347 No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT 12911 0.20827727543447694 No Hit
GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG 12819 0.20679315264461 No Hit
* 12743 0.20556713816602432 No Hit
CATGGGGTTGTGCCAATTAATTACCAAACATTGAGCCTGCAGGCTTTGAG 12464 0.20106637448805828 No Hit
AAGACAGGGGAAAGGAAGAAAAGGGAGTGAAGAGGGAATGGGAGAGGGAA 12111 0.19537185987041672 No Hit
GATACAACATTCAAGACAGAGGAACTGGATGCCAAGCCAGACACAGACCC 11454 0.18477328733843226 No Hit
CATGTATTTTGAACCGGCACCCCTGTTACCACAGAGTGTGGGAGGAACTG 11404 0.18396669886567849 No Hit
TGAGACCGCACCAGCGACACA 10785 0.1739811335729869 No Hit
TGTGTCGCTGGTGCGGTCTCA 10643 0.17169042231036621 No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG 10448 0.16854472726662653 No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA 10354 0.16702834093784946 No Hit
CATGTAGACTTACCTTTCCTCATAGAGCTATCCTGGTTAATAACAGGCCA 10094 0.16283408087952989 No Hit
ACACTTACACCAAGGAATCTCCCAGGCATTATCAAAGCTACAGGAGACAA 9998 0.16128543101184264 No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT 9985 0.16107571800892667 No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT 9929 0.16017233891944246 No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC 9446 0.1523806942726411 No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA 9437 0.15223550834754543 No Hit
CACTCAGTATGAGGAGCCGGCTGTCATCACATAGGGACAGGGCTCTCCCT 8963 0.14458904962583974 No Hit
CATGGAGGGGAGCACTCAGCACCTCCCTCACCCCACACCCTTGGCTGCTC 8877 0.14320171745270327 No Hit
GCCTCAGCCTTCCTTCGCAGAAAGTTGAGCAGGTCGCCATAGCAACAGTA 8814 0.14218541597703352 No Hit
CATGTAATTCAGGCAGTTATGGGAGCCCTAGAGGGCCTGAGAGTTGCTATTGGAC 8697 0.14029799895078973 No Hit
GTCCAATAGCAACTCTCAGGCCCTCTAGGGCTCCCATAACTGCCTGAATTACATG 8655 0.13962046463367656 No Hit
ACCTATGTTACACCATCTTCAGTGCCAGTCTTGGGCAAAATTGTGCAAGA 8404 0.13557139050045267 No Hit
AGGACAACAGCTGATCCATACATTTGCTGACAGGTGTACCTGCGTTTCCC 8341 0.13455508902478291 No Hit
TAAAACTGCATCAAGTCATGGGGCATGTGGAAGGTAGGGAGGCAAGATGA 8287 0.13368397347420885 No Hit
TGAGTAGGGGGAGCAAATCGTGCCTTGTCATTTTATTTGGAGGTTTCCTG 8270 0.13340973339347256 No Hit
CTTGGGGGAGTGTGCCTCCAGCAGAGAAGTAATTCTGGCCTGGCAACCTA 8269 0.1333936016240175 No Hit
GCCCTCCTCCTGCCACGGGCCTGCTCCCCTCCTTCTCTCATGGGGGTCTG 8113 0.13087704558902574 No Hit
GAGGGAGAGGCCGGAGCTGCCCAGGACAGGCTCTGGGTCCTGCCCTTGCA 7841 0.12648920429724528 No Hit
CATGGCTCCACTCTCCACCGTCTCCACGCACACCTTCTCCAGCATCTGGG 7758 0.125150267432474 No Hit
TAATCCAGAGCAAGGCCTTGACTCCCTTCAGTTCTATGAAGAGAATGAGG 7682 0.1239242529538883 No Hit
AACTTTATAAACCAACTTCTGCTAGCTTCCAACTTCTCTTCTGCAGCTTC 7670 0.1237306717204274 No Hit
CACCAAAAACCCCAAGACAGAAATCTTAGGTATTCAGTTTCTTTTTCACA 7412 0.119568675201018 No Hit
ACAGATTTTGGGGTTGTGTTGTCACCCAAGAGATTGTTGTTTGCCATACT 7399 0.11935896219810199 No Hit
GACGCGACCGGACCGAGCGCCAAACGCGGCAGCCCAATCGCCATCGCTTT 7344 0.11847171487807287 No Hit
CACACTGTCATTAGTTGCAGCATCTCCTGTAGCAATTGGGCTAAATCTGT 7191 0.11600355415144635 No Hit
CATGGAATATATATTCTCCTGGCTGGTGCTTATGATTGCAAAACTTGGAT 7174 0.11572931407071008 No Hit
CGAGCCCGGCGGGCCCTGTGATTGGACGGGCGCCCGCCTCGCGTCCCGCC 7098 0.11450329959212435 No Hit
CAACACCCAACACTTATAAGCGGAAGAACACAGAAACAGCTCTAGACAAC 6843 0.11038969838108015 No Hit
TGATCCCTTTTTCCTTTTCTCTTTCCTTGACTCCCGCTTATTCTCCTTTT 6789 0.10951858283050608 No Hit
GACTGAAAGAGGGAGAAATAAAGACAAGGCCCCCGAGGAGCTGTCCAAAG 6705 0.10816351419627974 No Hit
AAACCTGGAGTTTCTTATGTTGTCCCAACCAAGGCCGACAAAAGGAGATC 6631 0.10696976325660418 No Hit
CATGATATACATACTCTCTGTAAAGTTACTCTTGGTTGCTTGATAGGTAG 6623 0.10684070910096358 No Hit
GTTTTTATACACTAAGGACACTCCCAGTGATGAAAATGAATTCCCCTCCA 6604 0.10653420548131715 No Hit
AGACTCTTACTCTTCTCAATCTTGCAGAGCTGAGCTTTGGCCTTTTTTAG 6598 0.1064374148645867 No Hit
TAAACATTCCACTTCCTCCATAGGCTCCATGTTGGCTCAGGCAGACAAGC 6576 0.10608251593657504 No Hit
CAGCTGGGTGGCCCCAGGAGAGGCGAGGCCCTGAGAGAAAGGCTTTCTAC 6538 0.10546950869728218 No Hit
CATGGTCACTCTCCCCAAAATATTATATTTTTTCTATAAAAAGAAAAAAA 6510 0.10501781915254008 No Hit
TAAATATAGCCCCAAAATGGTTGCTATAATAATCCCCATTTCATACTGGG 6442 0.10392085882959495 No Hit
TGAGATTTTTTTGTGTATGTTTTTGACTCTTTTGAGTGGTAATCATATGT 6418 0.10353369636267315 No Hit
TAAACATGTCTGCAGAAGAGCAGCTGTTGCCCTTGGCCGAAAACGAGCTG 6340 0.10227541834517727 No Hit
CAGGCTGGGTCCCAGCATGGCCTCAGCCTTCCTTCGCAGAAAGTTGAGCA 6329 0.10209796888117145 No Hit
ATCCCCTCTTGCCATCTGTTTTTCAGAGGACCTACTAACTATGAAGAGCC 6329 0.10209796888117145 No Hit
CTGAAATTATAAGTCCTCCATCACCAGACCGACCTCCTCATTCACAAACC 6310 0.10179146526152502 No Hit
AGCACTCAGCACCTCCCTCACCCCACACCCTTGGCTGCTCTAGGCCCTGT 6303 0.10167854287533948 No Hit
GTCGACAGCCAGGGGATCTCTGTGTTGGAGAATAACTGGGTGTTCGAATC 6276 0.10124298510005246 No Hit
GACAACTTGGCCACCGACAGCTGAAGGGCTTTTCACCTGTTGACACAATTGCCAGTCA 6254 0.10088808617204081 No Hit
TGACTGGCAATTGTGTCAACAGGTGAAAAGCCCTTCAGCTGTCGGTGGCCAAGTTGTC 6217 0.10029121070220302 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 3428110 3.4101398 6.3175015 2
TTTTT 3079605 3.3279376 4.2030964 60-64
GGGGG 878630 2.9946337 8.673318 4
GGAGG 1044585 2.7830267 5.335466 4
CCAGG 1042975 2.679488 5.197423 5
CCAGC 1043245 2.6318817 8.617593 5
CCTGG 1004525 2.623803 5.6777234 9
CCTCC 1029705 2.593508 9.488714 6
CCCCA 1033855 2.5611904 5.712842 1
CAGCC 998405 2.51876 6.2055593 6
TGGGG 896820 2.4292457 11.717546 3
CTCCC 901350 2.2702215 5.311533 3
AGCAG 1097620 2.244728 5.0248737 5
GCTGG 831075 2.2105916 8.096527 7
GAGGG 787770 2.0988095 6.599841 9
CTCCT 1009430 2.0576775 6.240259 4
GGGGA 770130 2.0518124 6.4604273 7
CTCCA 1000725 2.0064268 5.7075567 6
TCCAG 970130 1.9807805 6.2682366 7
AAGAA 1548190 1.9701793 5.2279854 2
CCCTG 751805 1.9283143 5.1928134 1
GCCAG 746190 1.917023 7.7880034 4
TGGCT 903380 1.9097095 7.3186803 3
CCCAC 768100 1.90283 6.0848346 9
CACCA 961150 1.8954275 5.8161135 8
TCCTC 927390 1.8904426 6.9166093 5
TGCAG 897090 1.8652639 5.968518 3
TGGTG 864000 1.8599806 5.4632506 9
TGTTT 1327490 1.8050239 5.4433503 3
CACAC 910340 1.795228 5.0742764 8
GCCCA 711135 1.79404 6.622166 4
TGCCA 878140 1.7929584 5.038635 3
GGCTC 683945 1.7864532 7.9010115 4
AGGGG 654730 1.7443588 8.503304 6
GGCCC 515550 1.6638527 5.4584756 1
GAAAG 1000950 1.6295133 5.189895 5
TGCCC 612925 1.5720992 11.825932 3
GCTCC 606010 1.5543628 7.704817 2
ATGGA 926940 1.5342274 10.711274 2
GATGG 714140 1.512117 6.261437 1
TAAAA 1491310 1.5082659 8.27185 1
CATGG 701700 1.4590015 42.580864 1
GGGGT 506360 1.371594 5.877875 4
TGCTC 650415 1.3501732 5.6423078 1
GCAGT 640535 1.3318249 5.4559455 4
TGGGT 603390 1.2989509 6.4585233 3
ATGTG 752480 1.2662679 8.507313 2
CTCAC 624025 1.2511533 5.0431023 7
ATGAA 965860 1.2496495 9.800461 2
TGAGA 753660 1.2474225 6.267607 1
ATGGG 583735 1.2359983 14.946752 2
AGACA 763480 1.2205212 5.2073774 2
ATGGC 586385 1.2192342 11.517153 2
ATGCC 591405 1.2075119 12.1077795 2
GGAGT 556095 1.1774733 5.2360616 7
GTCTT 669495 1.124793 5.0217743 9
GAGAC 548500 1.12173 6.1937995 2
CATGT 657110 1.0858524 33.144 1
CATGA 665270 1.0812799 31.075525 1
ATGTT 783835 1.0482956 8.059795 2
TAAAG 796525 1.0305605 6.1740746 1
ATGAT 773300 1.0172193 7.1545305 2
GTGTC 476550 1.0074077 5.402633 2
ATGCT 608430 1.0054104 8.147655 2
AGACT 610600 0.9924235 5.4251885 1
AGACC 492790 0.9896366 5.7291193 3
ATGGT 587235 0.9881948 7.749104 2
ATGAC 604915 0.98318344 7.015697 2
CACTC 485600 0.97361493 5.08052 1
ATGTA 688890 0.9061841 9.189401 2
CATGC 438360 0.8950294 32.691635 1
GCGCC 263785 0.85132265 5.6776032 8
CGCTG 319135 0.8335754 5.981787 6
TAACT 582995 0.75306684 5.503041 1
TAAGT 560055 0.7367112 6.2802434 1
ATGCA 445080 0.72339964 9.045281 2
TAAAC 544535 0.69183415 5.6122007 1
TAAGG 390505 0.64634544 5.136738 1
TGCCG 206700 0.539897 7.837444 5
CCGTC 173985 0.44625637 5.6695867 7
CGTCT 206025 0.42767993 5.063709 8
GCCGT 159620 0.41692483 5.7334085 6
GTCGC 129795 0.33902243 5.25273 4
Produced by FastQC (version 0.10.0)