##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	8016-8-26.h.sapiens.aligned.bam	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4677506	
Filtered Sequences	0	
Sequence length	1-100	
%GC	43	
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.98822000442116	24.0	24.0	31.0	23.0	31.0
2	31.010820192042253	31.0	31.0	32.0	27.0	33.0
3	32.67333581575309	33.0	33.0	34.0	29.0	35.0
4	33.89633990142407	34.0	33.0	36.0	29.0	37.0
5	35.42969663858494	36.0	35.0	37.0	33.0	37.0
6	35.75295552088024	36.0	36.0	38.0	33.0	38.0
7	35.770479658008576	37.0	35.0	37.0	34.0	37.0
8	35.61674768276815	36.0	36.0	37.0	32.0	38.0
9	35.63873838677729	37.0	36.0	37.0	31.0	38.0
10-14	36.008344429601635	37.0	36.0	37.4	32.4	38.0
15-19	36.999040881371435	38.0	37.2	38.4	33.4	39.4
20-24	36.623851978513926	37.4	36.0	38.8	32.8	39.8
25-29	36.281718275019266	37.6	36.2	38.0	32.8	38.2
30-34	36.126789030545595	37.6	35.6	38.2	32.6	38.6
35-39	36.16124209820556	37.8	36.4	38.0	31.6	38.6
40-44	36.081695499348974	37.4	36.0	38.4	32.4	38.8
45-49	36.03534797477495	37.6	35.8	38.2	32.0	38.6
50-54	36.25207725066748	37.6	36.2	38.4	33.0	39.0
55-59	35.97002561760614	37.6	35.8	38.2	32.4	38.6
60-64	35.97347999806942	37.6	35.6	38.2	32.6	38.6
65-69	35.78682462853915	37.4	36.0	38.2	31.6	38.6
70-74	35.84271691448048	37.4	36.0	38.4	32.0	39.0
75-79	35.56056096259424	37.4	35.4	37.6	31.6	38.4
80-84	35.66705619480368	37.2	35.8	38.2	31.2	38.6
85-89	35.37506464792208	37.2	35.4	37.6	31.0	38.2
90-94	35.388063606497084	37.0	35.0	38.2	30.8	38.4
95-99	35.214253177108766	37.4	35.2	37.6	31.0	38.2
100	34.12419738388834	36.0	33.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
1	3.0
2	9.0
3	52.0
4	0.0
5	6.0
6	43.0
7	148.0
8	173.0
9	4579.0
10	433.0
11	567.0
12	1004.0
13	1964.0
14	2661.0
15	3108.0
16	3405.0
17	4109.0
18	5376.0
19	6609.0
20	7845.0
21	8280.0
22	8540.0
23	9682.0
24	12380.0
25	16393.0
26	21978.0
27	29944.0
28	40776.0
29	53806.0
30	70367.0
31	92122.0
32	120061.0
33	163251.0
34	242022.0
35	439065.0
36	1343405.0
37	1963332.0
38	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.2051746319456	18.78644120196741	20.706963332547584	39.3014208335394
2	19.122239274537304	48.7669792226898	18.274243887008996	13.8365376157639
3	17.887871075877147	34.91597264488427	31.573625374617677	15.622530904620904
4	33.757307851344024	24.935107906647843	21.564892521400264	19.742691720607862
5	20.376954464323756	27.884615178758565	27.065138712828702	24.673291644088977
6	19.941416919114104	29.564948139690607	26.654059819496958	23.83957512169833
7	21.324144247261437	28.579536984522168	27.854608136612015	22.241710631604377
8	21.338355875084446	27.8726435684578	29.311615916491068	21.47738463996669
9	20.933791550961004	28.97306918350802	27.559391408740762	22.533747856790214
10-14	21.491750210958028	27.9075902308762	27.34913941376244	23.25152014440333
15-19	20.964762509987462	27.937194139021177	27.715898521437964	23.382144829553397
20-24	21.45158466125538	26.980559752916715	28.123499896430175	23.444355689397728
25-29	21.60896495799348	27.58013849029425	28.437077185384634	22.373819366327634
30-34	21.952765288498163	28.019179816067798	27.897361204853315	22.130693690580728
35-39	22.014296589782514	26.702221697300484	29.03585415854876	22.247627554368247
40-44	22.009665519688447	27.91953527852674	27.90653428766951	22.1642649141153
45-49	21.889730485962136	28.453025820185186	28.144430971538238	21.512812722314443
50-54	22.102648449048303	28.027605691598197	27.922950755403207	21.9467951039503
55-59	21.74873895552055	28.550519464086165	27.724962075956128	21.97577950443716
60-64	21.91502740744034	28.154816829988448	28.53144874281906	21.398707019752152
65-69	22.015201998609008	28.9361056493966	28.137762857624676	20.91092949436972
70-74	21.842815176403573	28.654426141787138	27.79766774466861	21.705090937140685
75-79	22.434892146196994	28.62678478053719	27.80816128197856	21.130161791287254
80-84	21.41311654525115	28.632351910048747	28.03104875991237	21.923482784787733
85-89	21.789661525248288	28.364866594756645	28.301552070190983	21.543919809804084
90-94	21.813886595500577	27.90015715847823	28.367663353776866	21.91829289224433
95-99	21.92719200204646	27.379195110350153	28.567858088979705	22.125754798623685
100	22.93255731242253	27.345119108630776	27.484407081613664	22.237916497333025
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	60.506595465485006
2	32.95877689030121
3	33.51040198049805
4	53.499999571951896
5	45.05024610841273
6	43.780992040812436
7	43.56585487886582
8	42.81574051505113
9	43.46753940775122
10-14	44.74327035536136
15-19	44.346907339540856
20-24	44.895940350653106
25-29	43.982784324321116
30-34	44.08345897907889
35-39	44.26192414415076
40-44	44.17393043380375
45-49	43.402543208276576
50-54	44.0494435529986
55-59	43.72451845995771
60-64	43.31373442719249
65-69	42.926131492978726
70-74	43.54790611354426
75-79	43.56505393748425
80-84	43.33659933003888
85-89	43.33358133505237
90-94	43.7321794877449
95-99	44.05294680067014
100	45.170473809755556
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4532.0
1	4536.0
2	4541.5
3	4537.0
4	4533.5
5	4541.5
6	4550.5
7	4569.0
8	4590.0
9	4601.5
10	4623.0
11	4660.5
12	4675.0
13	4740.5
14	4790.5
15	5158.0
16	5388.0
17	5238.0
18	5630.0
19	6658.0
20	8047.0
21	9475.5
22	13436.5
23	19875.5
24	31559.0
25	43952.5
26	56050.0
27	69355.5
28	72429.0
29	83754.5
30	108024.5
31	119235.0
32	125253.0
33	140236.5
34	159447.0
35	159660.5
36	150042.0
37	142630.5
38	158717.0
39	176818.0
40	181671.0
41	187035.0
42	172942.5
43	182477.0
44	191860.0
45	174882.0
46	155985.5
47	137149.5
48	133454.0
49	139721.0
50	141620.5
51	146359.0
52	122647.0
53	79639.5
54	70195.5
55	68985.0
56	54098.0
57	45494.0
58	60184.5
59	57478.5
60	56049.5
61	70578.5
62	62286.0
63	54961.5
64	51350.0
65	47143.5
66	47032.0
67	42825.5
68	30904.0
69	26426.0
70	24654.5
71	20273.0
72	25236.5
73	18695.0
74	8892.0
75	10468.5
76	10381.0
77	9699.5
78	8291.5
79	8196.0
80	7096.0
81	5250.0
82	4109.0
83	4244.5
84	4220.0
85	3000.5
86	4574.5
87	4265.5
88	2590.5
89	2742.0
90	2816.0
91	3008.0
92	2658.0
93	2295.5
94	2276.5
95	2275.5
96	2263.5
97	2253.0
98	2254.0
99	2259.5
100	2259.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05443071585584284
2	0.0081533427635338
3	0.002054416353154171
4	0.007939661992966444
5	0.004688375056303317
6	8.56533066780243E-4
7	3.6411231879789817E-4
8	1.499306570711046E-4
9	2.1418706584281753E-5
10-14	0.0014907564106595252
15-19	0.01885837433701697
20-24	0.017373862899781885
25-29	0.008902265510455904
30-34	0.008763563195099631
35-39	0.005689835530612671
40-44	0.01383021107993668
45-49	0.012682378111318195
50-54	0.011436083238626245
55-59	0.011644866480881404
60-64	0.056165352478849455
65-69	0.04540869250231885
70-74	0.028924589589046906
75-79	0.07548820103922951
80-84	0.06601016320411418
85-89	0.015313294929930117
90-94	0.006871442245527926
95-99	0.009583898104177473
100	0.00818731280170536
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
0-1	4576.0
2-3	187.0
4-5	2754.0
6-7	1164.0
8-9	9.0
10-11	42.0
12-13	53.0
14-15	117.0
16-17	103.0
18-19	857.0
20-21	14532.0
22-23	1018.0
24-25	1289.0
26-27	652.0
28-29	594.0
30-31	871.0
32-33	586.0
34-35	899.0
36-37	661.0
38-39	565.0
40-41	1261.0
42-43	787.0
44-45	808.0
46-47	964.0
48-49	754.0
50-51	10915.0
52-53	1610.0
54-55	13658.0
56-57	12704.0
58-59	31344.0
60-61	1578.0
62-63	7868.0
64-65	3998.0
66-67	5307.0
68-69	4888.0
70-71	21150.0
72-73	15664.0
74-75	13778.0
76-77	12480.0
78-79	4286.0
80-81	8318.0
82-83	9440.0
84-85	5703.0
86-87	2555.0
88-89	3750.0
90-91	8125.0
92-93	2495.0
94-95	2920.0
96-97	100892.0
98-99	0.0
100-101	4335977.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	97.06798252740354
#Duplication Level	Relative count
1	100.0
2	12.090123591818879
3	6.498231798461482
4	3.863064639615006
5	2.2385066899996353
6	1.2913339895730795
7	0.7911334718728353
8	0.488534033322396
9	0.33541142586313755
10++	7.743629005796785
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA	11525	0.2463919875249759	No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC	11406	0.2438478967210304	No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC	8540	0.1825759282831492	No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG	8517	0.18208421325381516	No Hit
CACTCAGTATGAGGAGCCGGCTGTCATCACATAGGGACAGGGCTCTCCCT	8364	0.17881323936302806	No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT	8139	0.17400298364128236	No Hit
GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG	8025	0.17156578740893116	No Hit
CATGCCCTCCAGCGGCCCCGGGGACACCAGCAGCTCTGCTGCGGAGCGGG	8010	0.1712451036941481	No Hit
GATACAACATTCAAGACAGAGGAACTGGATGCCAAGCCAGACACAGACCC	7256	0.1551254022977202	No Hit
CATGTATTTTGAACCGGCACCCCTGTTACCACAGAGTGTGGGAGGAACTG	7165	0.15317992109470305	No Hit
TAAAGAAACCCACAGAAACACACACACATACAATTAGCAAATGAATTCAA	6972	0.14905379063116114	No Hit
AAAAATAATAAACCGTTCATTTCTCAGGATGTGGTCATAGAATAAAGTTA	6951	0.14860483343046488	No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA	6721	0.14368768313712477	No Hit
CATGGGGTTGTGCCAATTAATTACCAAACATTGAGCCTGCAGGCTTTGAG	6695	0.14313183136483418	No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG	6659	0.14236219044935486	No Hit
AAGACAGGGGAAAGGAAGAAAAGGGAGTGAAGAGGGAATGGGAGAGGGAA	6560	0.14024567793178672	No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG	6219	0.1329554681490521	No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA	6145	0.1313734284894557	No Hit
GACGCGACCGGACCGAGCGCCAAACGCGGCAGCCCAATCGCCATCGCTTT	5973	0.1276962552266101	No Hit
CATGTAGACTTACCTTTCCTCATAGAGCTATCCTGGTTAATAACAGGCCA	5774	0.12344185127715496	No Hit
ACACTTACACCAAGGAATCTCCCAGGCATTATCAAAGCTACAGGAGACAA	5766	0.123270819962604	No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA	5759	0.12312116756237192	No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC	5749	0.12290737841918321	No Hit
AGCAACAAGAACAATACAAACCTCATTTCCTTTAGTCCTTCTGCCTCTAT	5714	0.12215911641802277	No Hit
CGAGCCCGGCGGGCCCTGTGATTGGACGGGCGCCCGCCTCGCGTCCCGCC	5714	0.12215911641802277	No Hit
TAACTGTGGAAAAACTAAGTTGTCACCAGCGGTACCTCATAGCATAACTT	5710	0.12207360076074729	No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT	5588	0.11946537321384516	No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT	5527	0.11816125944039409	No Hit
AGCCACCTCACCCACCCGGAATCTACATTTTCAATCAGTAAAAGTCACCA	5305	0.11341514046160496	No Hit
TAAATATAGCCCCAAAATGGTTGCTATAATAATCCCCATTTCATACTGGG	5303	0.11337238263296723	No Hit
CATGGTCACTCTCCCCAAAATATTATATTTTTTCTATAAAAAGAAAAAAA	5275	0.11277377303203888	No Hit
CATGTCATCTCAAAGGTCCCCAGGATTCGAACACCCAGTTATTCTCCAAC	5239	0.11200413211655955	No Hit
CATGGAATATATATTCTCCTGGCTGGTGCTTATGATTGCAAAACTTGGAT	5151	0.11012278765649901	No Hit
CACACTGTCATTAGTTGCAGCATCTCCTGTAGCAATTGGGCTAAATCTGT	5101	0.10905384194055551	No Hit
CATGGGCTCGGCCACGCGCTACCACACCTACCTGCCGCCGCCCTACCCCG	5052	0.10800627513893088	No Hit
GAGAACTGGTAGGAGCCGGCCGAGGCGCCGTAGTACAGGTGGTAGGAGGG	4924	0.10526977410611552	No Hit
GAAGCAGTAGTTTCACTTCTAGCTGGTCTCCCTCTGCAGCCTGAAGAAGG	4852	0.10373049227515689	No Hit
CATGGGTATTATTTCTTTGCTTTTTTTGTGTGGTGGGGGGCTTTATTTGC	4849	0.10366635553220027	No Hit
AGTTTGTTTGTTCATCCAGCAAATGTTTATGAGTGATGACCATGTGCCAG	4836	0.10338842964605498	No Hit
GTCCAAGTACAATCCCCCAAACATTAGTAAACTACTATGTGGCGCCAAGG	4832	0.10330291398877949	No Hit
AACTTTATAAACCAACTTCTGCTAGCTTCCAACTTCTCTTCTGCAGCTTC	4806	0.10274706221648888	No Hit
ACCTATGTTACACCATCTTCAGTGCCAGTCTTGGGCAAAATTGTGCAAGA	4777	0.10212707370124165	No Hit
TAATCCAGAGCAAGGCCTTGACTCCCTTCAGTTCTATGAAGAGAATGAGG	4763	0.10182776890077747	No Hit
GCCTCAGCCTTCCTTCGCAGAAAGTTGAGCAGGTCGCCATAGCAACAGTA	4699	0.1004595183843698	No Hit
TAAAACTGCATCAAGTCATGGGGCATGTGGAAGGTAGGGAGGCAAGATGA	4697	0.10041676055573205	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	2544015	3.2383292	5.5620093	2
TTTTT	2384830	3.231828	3.9432147	75-79
GGGGG	658410	3.0398018	7.8864846	4
GGAGG	785755	2.8036678	5.216853	4
CCCCA	783255	2.6409934	5.133613	1
CCAGC	765155	2.6167204	7.664691	5
CAGCC	755120	2.5824027	5.6900196	6
CCTCC	752100	2.5678973	9.449757	6
TGGGG	667475	2.4116387	9.635118	3
GGCAG	614905	2.1632345	5.464337	8
GAGGG	599360	2.1385882	7.218035	9
GGGGA	579210	2.0666902	5.134144	7
CCCTC	590405	2.0158215	5.097746	5
CTCCT	739915	2.0051923	5.108732	4
GCCCC	457470	1.9958885	5.005178	9
CTCCA	745580	1.9954026	5.1152844	6
GCCAG	573580	1.9895083	6.920629	4
GAGGA	702585	1.9374421	5.370381	1
TCCAG	713290	1.9361825	5.3976016	7
CCCAC	571245	1.9261342	5.419936	9
TCCTC	691520	1.8740404	5.79071	5
TGGCT	667495	1.8608437	6.220854	3
TGCAG	666605	1.8352385	5.333735	3
GCCCA	535360	1.8308545	5.09664	4
TGTTT	1045615	1.8106489	5.6046844	3
GGCTC	504815	1.7730538	6.371505	4
AGGGG	488645	1.7435437	6.715726	6
GAGCC	500470	1.7359204	5.3935933	2
TGCCC	453380	1.5700309	11.154391	3
TAAAA	1211155	1.5611295	7.053894	1
GCTCC	448165	1.5519717	7.0581236	2
ATGGA	690165	1.4893993	10.33417	2
GATGG	530990	1.4827027	5.431476	1
CATGG	531715	1.4638711	40.595974	1
GGGGT	372120	1.3444983	5.0581555	4
ATGTG	590475	1.2903206	8.119199	2
TGGGT	453415	1.2820398	5.9049945	3
ATGAA	740430	1.2349026	8.680352	2
ATGCC	447605	1.2149967	11.11997	2
ATGGG	434490	1.2132422	13.802574	2
TGAGA	556750	1.2014853	6.113181	1
ATGGC	434570	1.1964201	10.279702	2
CATGT	509080	1.0968275	32.56657	1
GAGAC	400470	1.0888199	5.161602	2
CATGA	504830	1.0741369	28.494284	1
ATGTT	624410	1.0678109	8.32599	2
TAAAG	616965	1.0289855	5.3909307	1
ATGCT	463230	0.9980424	7.6077805	2
ATGGT	456035	0.996539	7.353237	2
ATGAG	461260	0.9954146	5.136625	2
ATGAT	571690	0.96548873	6.0468082	2
ATGAC	452895	0.9636336	7.0195823	2
ATGTA	551765	0.9318387	8.55532	2
GCGCC	205595	0.90976673	5.1616716	8
CATGC	332500	0.9025511	30.070068	1
ATGCA	362255	0.7707771	8.190847	2
TAAGT	448025	0.7566393	5.9111114	1
TGCCG	155815	0.54726654	6.8719544	5
>>END_MODULE