FastQCFastQC Report
Wed 20 Mar 2013
8016-8-26.h.sapiens.aligned.bam

Summary

[OK] Basic Statistics

Measure Value
Filename 8016-8-26.h.sapiens.aligned.bam
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 4677506
Filtered Sequences 0
Sequence length 1-100
%GC 43

[WARN] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
AGATTGTTGAGGGATTGCGAGCTGCAGCCAGAAGGAATGGAGATGTTATA 11525 0.2463919875249759 No Hit
GAGGAAAGGCAGATAGTAATCCTTAAAGGCTGAGGATTGGAACCCAGATC 11406 0.2438478967210304 No Hit
GATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACAC 8540 0.1825759282831492 No Hit
AGACTCCTCACCTTTGACATAGAGGTGGATGGCGGCGCTGCCTCCCAGGG 8517 0.18208421325381516 No Hit
CACTCAGTATGAGGAGCCGGCTGTCATCACATAGGGACAGGGCTCTCCCT 8364 0.17881323936302806 No Hit
CATGCCAGCTACGATTACGAAACCAATCAGCAAAGGAGTGAACAGGGTGT 8139 0.17400298364128236 No Hit
GGCTGTGAGTTGGGAGGTGGGGTCAGTTTGGGACTGAGTGGCTGTGGTAG 8025 0.17156578740893116 No Hit
CATGCCCTCCAGCGGCCCCGGGGACACCAGCAGCTCTGCTGCGGAGCGGG 8010 0.1712451036941481 No Hit
GATACAACATTCAAGACAGAGGAACTGGATGCCAAGCCAGACACAGACCC 7256 0.1551254022977202 No Hit
CATGTATTTTGAACCGGCACCCCTGTTACCACAGAGTGTGGGAGGAACTG 7165 0.15317992109470305 No Hit
TAAAGAAACCCACAGAAACACACACACATACAATTAGCAAATGAATTCAA 6972 0.14905379063116114 No Hit
AAAAATAATAAACCGTTCATTTCTCAGGATGTGGTCATAGAATAAAGTTA 6951 0.14860483343046488 No Hit
CCACCGAGCAAACGCATCTTGCTTTTCACATCTCTCCTCCTACAGCCTTA 6721 0.14368768313712477 No Hit
CATGGGGTTGTGCCAATTAATTACCAAACATTGAGCCTGCAGGCTTTGAG 6695 0.14313183136483418 No Hit
CATGAATCCAGAAGCACTTAACACTGGTGGTGGTGATTTGTCACATATGG 6659 0.14236219044935486 No Hit
AAGACAGGGGAAAGGAAGAAAAGGGAGTGAAGAGGGAATGGGAGAGGGAA 6560 0.14024567793178672 No Hit
CATGCATCTAAATCTTCTCTGGAGATTATCTCCCTACTGTGTAGGTTAAG 6219 0.1329554681490521 No Hit
GATGGCACACACACACAACACAGGGAGCTGGAGCCTCAGTTTCACGCTGA 6145 0.1313734284894557 No Hit
GACGCGACCGGACCGAGCGCCAAACGCGGCAGCCCAATCGCCATCGCTTT 5973 0.1276962552266101 No Hit
CATGTAGACTTACCTTTCCTCATAGAGCTATCCTGGTTAATAACAGGCCA 5774 0.12344185127715496 No Hit
ACACTTACACCAAGGAATCTCCCAGGCATTATCAAAGCTACAGGAGACAA 5766 0.123270819962604 No Hit
CATGGTAAAGGGTTTGTGCTAACTGACCTCTTCTTTCCTTGCAGGGCTAA 5759 0.12312116756237192 No Hit
GTGCTAATGGACCAGGGTCACAGTTTCAAAACTTGAACAATCCAGTTAGC 5749 0.12290737841918321 No Hit
AGCAACAAGAACAATACAAACCTCATTTCCTTTAGTCCTTCTGCCTCTAT 5714 0.12215911641802277 No Hit
CGAGCCCGGCGGGCCCTGTGATTGGACGGGCGCCCGCCTCGCGTCCCGCC 5714 0.12215911641802277 No Hit
TAACTGTGGAAAAACTAAGTTGTCACCAGCGGTACCTCATAGCATAACTT 5710 0.12207360076074729 No Hit
CATGTGTGCTTACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACT 5588 0.11946537321384516 No Hit
TTTGCCCAAGACTGGACAGCGGGCACACTTACCAGTGTGCTTCCTGCTGT 5527 0.11816125944039409 No Hit
AGCCACCTCACCCACCCGGAATCTACATTTTCAATCAGTAAAAGTCACCA 5305 0.11341514046160496 No Hit
TAAATATAGCCCCAAAATGGTTGCTATAATAATCCCCATTTCATACTGGG 5303 0.11337238263296723 No Hit
CATGGTCACTCTCCCCAAAATATTATATTTTTTCTATAAAAAGAAAAAAA 5275 0.11277377303203888 No Hit
CATGTCATCTCAAAGGTCCCCAGGATTCGAACACCCAGTTATTCTCCAAC 5239 0.11200413211655955 No Hit
CATGGAATATATATTCTCCTGGCTGGTGCTTATGATTGCAAAACTTGGAT 5151 0.11012278765649901 No Hit
CACACTGTCATTAGTTGCAGCATCTCCTGTAGCAATTGGGCTAAATCTGT 5101 0.10905384194055551 No Hit
CATGGGCTCGGCCACGCGCTACCACACCTACCTGCCGCCGCCCTACCCCG 5052 0.10800627513893088 No Hit
GAGAACTGGTAGGAGCCGGCCGAGGCGCCGTAGTACAGGTGGTAGGAGGG 4924 0.10526977410611552 No Hit
GAAGCAGTAGTTTCACTTCTAGCTGGTCTCCCTCTGCAGCCTGAAGAAGG 4852 0.10373049227515689 No Hit
CATGGGTATTATTTCTTTGCTTTTTTTGTGTGGTGGGGGGCTTTATTTGC 4849 0.10366635553220027 No Hit
AGTTTGTTTGTTCATCCAGCAAATGTTTATGAGTGATGACCATGTGCCAG 4836 0.10338842964605498 No Hit
GTCCAAGTACAATCCCCCAAACATTAGTAAACTACTATGTGGCGCCAAGG 4832 0.10330291398877949 No Hit
AACTTTATAAACCAACTTCTGCTAGCTTCCAACTTCTCTTCTGCAGCTTC 4806 0.10274706221648888 No Hit
ACCTATGTTACACCATCTTCAGTGCCAGTCTTGGGCAAAATTGTGCAAGA 4777 0.10212707370124165 No Hit
TAATCCAGAGCAAGGCCTTGACTCCCTTCAGTTCTATGAAGAGAATGAGG 4763 0.10182776890077747 No Hit
GCCTCAGCCTTCCTTCGCAGAAAGTTGAGCAGGTCGCCATAGCAACAGTA 4699 0.1004595183843698 No Hit
TAAAACTGCATCAAGTCATGGGGCATGTGGAAGGTAGGGAGGCAAGATGA 4697 0.10041676055573205 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 2544015 3.2383292 5.5620093 2
TTTTT 2384830 3.231828 3.9432147 75-79
GGGGG 658410 3.0398018 7.8864846 4
GGAGG 785755 2.8036678 5.216853 4
CCCCA 783255 2.6409934 5.133613 1
CCAGC 765155 2.6167204 7.664691 5
CAGCC 755120 2.5824027 5.6900196 6
CCTCC 752100 2.5678973 9.449757 6
TGGGG 667475 2.4116387 9.635118 3
GGCAG 614905 2.1632345 5.464337 8
GAGGG 599360 2.1385882 7.218035 9
GGGGA 579210 2.0666902 5.134144 7
CCCTC 590405 2.0158215 5.097746 5
CTCCT 739915 2.0051923 5.108732 4
GCCCC 457470 1.9958885 5.005178 9
CTCCA 745580 1.9954026 5.1152844 6
GCCAG 573580 1.9895083 6.920629 4
GAGGA 702585 1.9374421 5.370381 1
TCCAG 713290 1.9361825 5.3976016 7
CCCAC 571245 1.9261342 5.419936 9
TCCTC 691520 1.8740404 5.79071 5
TGGCT 667495 1.8608437 6.220854 3
TGCAG 666605 1.8352385 5.333735 3
GCCCA 535360 1.8308545 5.09664 4
TGTTT 1045615 1.8106489 5.6046844 3
GGCTC 504815 1.7730538 6.371505 4
AGGGG 488645 1.7435437 6.715726 6
GAGCC 500470 1.7359204 5.3935933 2
TGCCC 453380 1.5700309 11.154391 3
TAAAA 1211155 1.5611295 7.053894 1
GCTCC 448165 1.5519717 7.0581236 2
ATGGA 690165 1.4893993 10.33417 2
GATGG 530990 1.4827027 5.431476 1
CATGG 531715 1.4638711 40.595974 1
GGGGT 372120 1.3444983 5.0581555 4
ATGTG 590475 1.2903206 8.119199 2
TGGGT 453415 1.2820398 5.9049945 3
ATGAA 740430 1.2349026 8.680352 2
ATGCC 447605 1.2149967 11.11997 2
ATGGG 434490 1.2132422 13.802574 2
TGAGA 556750 1.2014853 6.113181 1
ATGGC 434570 1.1964201 10.279702 2
CATGT 509080 1.0968275 32.56657 1
GAGAC 400470 1.0888199 5.161602 2
CATGA 504830 1.0741369 28.494284 1
ATGTT 624410 1.0678109 8.32599 2
TAAAG 616965 1.0289855 5.3909307 1
ATGCT 463230 0.9980424 7.6077805 2
ATGGT 456035 0.996539 7.353237 2
ATGAG 461260 0.9954146 5.136625 2
ATGAT 571690 0.96548873 6.0468082 2
ATGAC 452895 0.9636336 7.0195823 2
ATGTA 551765 0.9318387 8.55532 2
GCGCC 205595 0.90976673 5.1616716 8
CATGC 332500 0.9025511 30.070068 1
ATGCA 362255 0.7707771 8.190847 2
TAAGT 448025 0.7566393 5.9111114 1
TGCCG 155815 0.54726654 6.8719544 5
Produced by FastQC (version 0.10.0)